Population variability based on the morphometry and chemical composition of the acorn in Holm oak (Quercus ilex subsp. ballota [Desf.] Samp.)     

José Valero Galván  Jesús J. Jorrín Novo  Augusto Gómez Cabrera  David Ariza  Juan García-Olmo  Rafael Ma Navarro Cerrillo 《European Journal of Forest Research》2012,131(4):893-904

We have studied in this work the natural variability in Holm oak (Quercus ilex subsp. ballota) by analyzing acorn morphometry and chemical composition in 13 populations from the Andalusia region (southern Spain). Acorn morphometry showed statistic significant differences between the populations with a higher acorn weight, length, and diameter in the northern provenances (TJA, GSE, CTH, CHU, APS, and PCO), while the Southern provenances (RMA, SAA, CCO, BCA, and SCA) showed lower value that even decreased with the altitude. Acorn chemical composition also showed statistic significant differences between populations. Morphometry and chemical composition data were subjected to clustering analysis, and two major clusters were detected. This analysis showed that northern populations (CTH, TJA, SCA, CHU, PCO, GSE, and APS) showed a tendency to present values highest in acorn weight (5.42?g vs. 3.21?g); acorn length (37.21?mm vs. 28.73?mm); acorn diameter (16.12?mm vs. 13.79?mm); ash (1.88% vs. 1.55%); protein (4.82% vs. 4.38%); fat (12.55% vs. 9.92%); and oleic acid (66.93 vs. 66.4%). On the contrary, Southern populations (SSA, VJA, CCO, BCA, RG, and RMA) showed a tendency to present values lowest in sugar (10.28% vs. 11.25%); energy (20,076.71?kJ/kg vs. 20,149.26?kJ/kg); palmitic (16.11% vs. 16.39%) and linoleic acids (13.07% vs. 13.11%). The populations studied here seemed to integrate acorn morphology and chemical composition related to environmental conditions of the original provenances. These data have provided biochemical markers that help establish phylogenic associations between populations and also reveal potentially account as an alternative source for dietary nutrition.  相似文献   

Geographically structured and temporally unstable growth responses of Juniperus thurifera to recent climate variability in the Iberian Peninsula     

Lucía DeSoto  Jesús Julio Camarero  José Miguel Olano  Vicente Rozas 《European Journal of Forest Research》2012,131(4):905-917

Geographically structured tree-ring networks are needed to fully understand the spatiotemporal variability in climatic sensitiveness of trees and to study their future responses to global warming. We aim to identify the spatially constrained structure of radial-growth patterns of the Spanish juniper (Juniperus thurifera L.) and to assess whether their climate–growth responses were unstable during the late twentieth century. Tree–ring width chronologies were built for 13?J. thurifera stands in Spain using dendrochronological methods and related to monthly climatic data. Sites were grouped according to their growth patterns using hierarchical cluster analysis. The relationships among geographical, climatic and stand features and their influence on radial growth were evaluated using redundancy analysis. The climate–growth relationships and their temporal stability were assessed using Pearson’s and moving bootstrapped correlations, respectively. Stands formed three geographical groups according to their high-frequency growth variation: North West and Centre, North East and South East. We found that J. thurifera radial-growth patterns depended on geographical and climatic factors, but not on the stand structure, and responded to a northwest–southeast gradient of decreasing rainfall and influence of Atlantic Westerlies and Mediterranean cyclonic activity. The positive response to June precipitation was unstable during the late twentieth century and started earlier in populations from western mesic sites than in eastern xeric sites. This pattern may be related to either decreasing water availability in western than in eastern sites or the resilience of J. thurifera growth from xeric sites in response to the increasing summer aridity.  相似文献   

From points to numbers: a database-driven approach to convert terrestrial LiDAR point clouds to tree volumes   总被引：2，自引：0，他引：2  

Rüdiger Hildebrandt  André Iost 《European Journal of Forest Research》2012,131(6):1857-1867

Data provided by airborne LiDAR (Light Detection and Ranging) techniques are commonly used for measuring and monitoring of forest resources. In the last years, terrestrial laser scanning systems have also gained increasing attention as a ground-based application for individual tree measurements. The database-driven approach described in this article focuses on the calculation of single tree stem volumes from terrestrial laser scan data. A straightforward way of determining the volume of upright cylindrical objects is presented. Three-dimensional point data are split up into cylindrical sections whose diameters are used as basis for volume calculations. Tests conducted with a simple tree stem model show that results produced by the algorithm differ by less than 1?% from results obtained by manual section-wise cubing, which was applied to gather reference data. The method we present offers a perspective on non-destructive techniques of volume data acquisition for forest resource assessments. It is capable of reducing the error of single tree biomass estimations which in turn would improve the reliability of biomass inventories. Also, other tasks such as forest inventories or forest management plans can benefit from more accurate estimations and smaller prediction errors.  相似文献   

Impact of the pinewood nematode, Bursaphelenchus xylophilus, on gross calorific value and chemical composition of Pinus pinaster woody biomass     

Valeria Reva  Luís Fonseca  José L. Lousada  Isabel Abrantes  Domingos X. Viegas 《European Journal of Forest Research》2012,131(4):1025-1033

The pinewood nematode (PWN), Bursaphelenchus xylophilus, the causal agent of the pine wilt disease, has been detected in several regions of Portugal affecting Pinus pinaster, a coniferous species of a great economic value. The nematodes, migrating through resin canals and feeding on parenchyma cells, induce rapid metabolic changes in ray parenchyma cells, cavitation areas, and denaturation and necrosis of parenchyma and cambial cells. To understand how anatomic changes and biochemical incidences of tree defense reactions affect the technological parameters of the wood, the gross calorific value (GCV) and chemical composition of PWN-infected and -uninfected P. pinaster wood were evaluated. The GCV was determined using Parr 6300 Automatic Isoperibol Calorimeter, and chemical composition analysis was performed by determining the contents of carbon, hydrogen, nitrogen, sulfur and Ash on complete and instant oxidation of samples by “flash” combustion. The Student’s t test with Welch correction was used for statistical data analysis. The difference between the GCV and chemical composition for PWN-infected and -uninfected P. pinaster wood was statistically significant for the GCV and for hydrogen and nitrogen contents. The carbon, oxygen, sulfur and Ash contents did not differ statistically. The GCV of PWN-infected wood varied between the highest value of hardwood and the lowest value of softwood. This interdisciplinary study stresses the important technological and economic aspects, namely the impact of PWN on wood properties and the suitability of infected P. pinaster wood for use in the wood-processing and energy industries.  相似文献   

Influence of the stage for anther excision and heterostyly in embryogenesis induction from eggplant anther cultures     

Pablo?Salas  Alba?Rivas-Sendra  Jaime?Prohens  José?M.?Seguí-SimarroEmail author 《Euphytica》2012,184(2):235-250

In this work we address two aspects of eggplant flower biology potentially involved on the efficiency of anther culture: the selection of the best floral stage to extract anthers for culture, and the effect of heterostyly in the identification of suitable buds and anthers. For 12 different accessions, we determined morphological criteria (length ranges) to identify buds and anthers enriched in vacuolate microspores and young bicellular pollen, the stages most responsive to embryogenesis induction. While these microspore/pollen stages were the most responsive when isolated and cultured in liquid medium, we observed that culture of anthers containing these stages is not the best choice. Instead, the highest response was found for younger anthers, containing mostly young and mid microspores. We analyzed eggplant anther walls and found that their particular thickness may be behind this apparent discrepancy, since they may delay the diffusion of inducing factors to the anther locule, reducing their effect over inducible microspores. Thus, the culture of younger anthers would allow for younger microspores to grow up to the inducible stages while factors are entering the locule. We also analyzed the embryogenic response of short and long-styled buds present in Cristal, a heterostylic cultivar. Our results demonstrated that each floral morph produced buds and anthers of different lengths, but equally useful for anther culture, since similar amounts of embryos were produced. The practical application of these results may improve the efficiency of anther culture not only in these cultivars, but in others also presenting thick anther walls and heterostyly.  相似文献   

Immunology. HD6 defensin nanonets     

AJ Ouellette  ME Selsted 《Science (New York, N.Y.)》2012,337(6093):420-421

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A PCR‐based method for discriminating between high molecular weight glutenin subunits Bx7 and Bx7* in Triticum aestivum L.     

Araceli Espí  Patricia Giraldo  Marta Rodriguez‐Quijano  José M. Carrillo 《Plant Breeding》2012,131(5):571-573

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Transfer of architectural traits from perennial Helianthus mollis Lam. to sunflower (H. annuus L.) and localisation of introgression     

Catherine Breton  Alain Gil  Julie Wargnier  Hervé Serieys  André Bervillé 《Euphytica》2012,186(2):557-572

Common sunflower (i.e., Helianthus annuus) is occasionally crossed with other Helianthus species to gain favourable agronomic traits. An interspecific cross between H. annuus and Helianthus mollis was used to examine events that occurred during the five first generations. First generation hybrid plants were verified using GISH, and F₁ hybrid (#17) was back-crossed to common sunflower to obtain BC₁ (#4) and BC₂ (#21). Some BC₂ progeny possessed new traits, i.e., compact (Compact plant architecture is due to short inter-node length, short petiole length and short leaf lamina length) architecture without and with corrugated leaves, which were not observed in the parents. These traits were selected for and fixed after the BC₂-S₃ (#18) to produce a H. mollis introgression line (namely HM374). Although the size of the population was small, we tentatively construct a genetic map using data from the BC₂ generation. Two linkage groups were identified carrying H. mollis specific AFLP markers that were associated with compact plant architecture, and one unlinked marker was shown to explain variation for the corrugated leaves. Introgression line HM374 was used to test the inheritance of compact plant architecture in a cross with an elite high oleic line, OL75HO. Microsatellite (SSR) markers were used to genotype F₁ and F₂ offspring’s. Our data suggested that the line HM374 was still heterozygous for introgression after eight generations. HM374 displays new architecture traits (i.e. compact architecture with or without corrugated leaves) and carries H. mollis fragments anchored on LG1 and LG11 of sunflower public map.  相似文献   

Stabilization of coxo-femoral luxation using tenodesis of the deep gluteal muscle. Technique description and reluxation rate in 65 dogs and cats (1995-2008)     

Rochereau P  Bernardé A 《Veterinary and comparative orthopaedics and traumatology》2012,25(1):49-53

This retrospective study documents deep gluteal tenodesis (DGT) used to stabilize coxo- femoral luxation (CFL) in dogs and cats, and to report reluxation rate and clinical outcome after DGT. Medical records (1995-2008) of 65 dogs and cats with traumatic CFL treated by capsulorrhaphy and DGT were reviewed. Animals with radiographic evidence of pre-existing hip dysplasia or articular fractures had been excluded. Reluxation rate and outcome were assessed by clinical examination, performed two and ten weeks postoperatively. Surgical treatment was performed between one and 20 days after the initiating event. No perioperative complications occurred. All hip joints were correctly reduced and stabilized immediately after DGT completion. Except for five patients, placement of the screw was considered correct. In two of these patients, the screws were too long and were protruding into the pelvic canal. In two dogs, the screws were not tightened adequately, and in one dog the screw was too short. Twenty-six dogs and eight cats were re-examined between eight and 13 weeks postoperatively. Re- luxation did not occur in any of them. Outcomes were good in two cases and excellent in 32 cases; all but two had a normal range-of-motion of the reconstructed hip, and were free of lameness and did not show any signs of pain. Traumatic CFL can be stabilized safely and effectively by DGT in dogs and cats. This technique should be considered among other capsular reinforcement techniques in the presence of an intact deep gluteal muscle.  相似文献   

Infectiousness of pigs infected by the Porcine Reproductive and Respiratory Syndrome virus (PRRSV) is time-dependent     

Céline Charpin  Sophie Mahé  André Keranflec’h  Catherine Belloc  Roland Cariolet  Marie-Frédérique Le Potier  Nicolas Rose 《Veterinary research》2012,43(1):69

The time-dependent transmission rate of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and the correlation between infectiousness, virological parameters and antibody responses of the infected pigs were studied in experimental conditions. Seven successive transmission trials involving a total of 77 specific pathogen-free piglets were carried out from 7 to 63 days post-inoculation (dpi). A semi-quantitative real time RT-PCR was developed to assess the evolution of the viral genome load in blood and nasal swabs from inoculated and contact pigs, with time. Virus genome in blood was detectable in inoculated pigs from 7 to 77 dpi, whereas viral genome shedding was detectable from nasal swabs from 2 to 48 dpi. The infectiousness of inoculated pigs, assessed from the frequency of occurrence of infected pigs in susceptible groups in each contact trial, increased from 7 to 14 dpi and then decreased slowly until 42 dpi (3, 7, 2, 1 and 0 pigs infected at 7, 14, 21, 28 and 42 dpi, respectively). These data were used to model the time-dependent infectiousness by a lognormal-like function with a latency period of 1 day and led to an estimated basic reproduction ratio, R₀ of 2.6 [1.8, 3.3]. The evolution of infectiousness was mainly correlated with the time-course of viral genome load in the blood whereas the decrease of infectiousness was strongly related to the increase in total antibodies.  相似文献