全文获取类型
收费全文 | 8576篇 |
免费 | 565篇 |
国内免费 | 645篇 |
专业分类
林业 | 701篇 |
农学 | 629篇 |
基础科学 | 349篇 |
1416篇 | |
综合类 | 3075篇 |
农作物 | 525篇 |
水产渔业 | 481篇 |
畜牧兽医 | 1542篇 |
园艺 | 521篇 |
植物保护 | 547篇 |
出版年
2024年 | 36篇 |
2023年 | 165篇 |
2022年 | 355篇 |
2021年 | 448篇 |
2020年 | 433篇 |
2019年 | 404篇 |
2018年 | 321篇 |
2017年 | 413篇 |
2016年 | 366篇 |
2015年 | 497篇 |
2014年 | 472篇 |
2013年 | 638篇 |
2012年 | 773篇 |
2011年 | 807篇 |
2010年 | 662篇 |
2009年 | 593篇 |
2008年 | 556篇 |
2007年 | 502篇 |
2006年 | 385篇 |
2005年 | 307篇 |
2004年 | 175篇 |
2003年 | 122篇 |
2002年 | 123篇 |
2001年 | 45篇 |
2000年 | 48篇 |
1999年 | 30篇 |
1998年 | 22篇 |
1997年 | 11篇 |
1996年 | 16篇 |
1995年 | 6篇 |
1994年 | 9篇 |
1993年 | 5篇 |
1992年 | 8篇 |
1991年 | 8篇 |
1990年 | 3篇 |
1989年 | 3篇 |
1988年 | 3篇 |
1987年 | 3篇 |
1986年 | 4篇 |
1985年 | 2篇 |
1984年 | 2篇 |
1982年 | 2篇 |
1981年 | 1篇 |
1962年 | 1篇 |
1956年 | 1篇 |
排序方式: 共有9786条查询结果,搜索用时 15 毫秒
991.
992.
993.
食用菌遗传育种及种质鉴定研究进展 总被引:4,自引:1,他引:3
伴随着科学尤其是分子生物学的持续发展,越来越多的新技术不断涌现并应用于食用菌的育种以及种质鉴定.主要对食用菌育种的遗传学基础、育种技术、种质评价及鉴定的手段进行综述,并针对当前存在的问题,对食用菌育种研究的趋向进行了展望. 相似文献
994.
AgNO_3对梨叶片不定梢再生过程中抗氧化酶活性的影响 总被引:1,自引:0,他引:1
为探讨AgNO3在离体培养物形态发生中的作用,以金水和金花梨组培苗为外植体,研究了不同质量浓度AgNO3对梨叶片再生不定梢过程中抗氧化酶活性的影响。结果表明,0.1mg/LAgNO3能明显提高金水叶片不定梢再生率和再生数;金花叶片不定梢再生有效促进的AgNO3为0.1~0.5mg/L。经AgNO3处理后,2品种抗氧化物酶活性与对照差异显著。在暗培养期间,叶片中过氧化氢酶(CAT)和过氧化物酶(POD)活性均有较大幅度变化,特别是经0.1mg/LAgNO3处理后,金花叶片中POD活性及金水叶片中CAT活性增幅均高于其余3个处理。叶片中O·2产生速率和H2O2含量也增加但上升幅度不及对照。在进入光培养阶段后,添加适当的AgNO3可提高叶片原有的超氧化物歧化酶(SOD)峰值,且H2O2含量和O·2产生速率随之下降,但较高含量AgNO3处理易引起H2O2含量和O·2产生速率回升。虽然品种间抗氧化物酶活性变化不完全一致,但通过比较发现0.1mg/LAgNO3对提高金花和金水叶片抗氧化物酶活性效果最佳。 相似文献
995.
AIM: To investigate the effect of irbesartan on the fatty liver of db/db mice and whether autophagy is involved in the process. METHODS: Male db/db mice (n=24) were randomly divided into model group and irbesartan group, and 12 db/m mice with similar age and weight were selected as normal control group. After 16 weeks of intervention respectively, the fatty liver-related parameters including body weight, liver index, blood lipid, liver function and pathological changes in the liver were observed. The protein levels of p-PI3K, p-Akt, and p-mTOR, as well as Atg-7, beclin-1 and LC3B in the liver tissues were detected by Western blot, and the autophagosomes in the liver were observed under electron microscope. RESULTS: Compared with the model group, the body weight, liver index, blood lipids, alanine and aspartate aminotransferase were decreased in irbesartan group (P<0.05). Moreover, the pathological changes in the liver were significantly ameliorated in irbesartan group than that of model group. Importantly, the protein levels of p-PI3K, p-Akt and p-mTOR were decreased with irbesartan administration, while the expression of Atg-7, beclin-1 and LC3B-Ⅱ was increased(P<0.05), which resulted in a distinct increase in autophagosomes. CONCLUSION: Irbesartan alleviates hepatic steatosis in db/db mice by inhibiting the PI3K/Akt/mTOR signaling pathway and upregulating the protein expression of Atg-7, beclin-1 and LC3B-Ⅱ, thereby inducing autophagy in hepatocytes. 相似文献
996.
LUO Hui-juan WANG Ning ZHOU Juan TANG Xiao-mei CHEN Yu-yi LUO Wen-bin XIAO Xiao-min 《园艺学报》2018,34(11):2079-2084
AIM: To evaluate the effects of transplantation of Wharton jelly-derived mesenchymal stem cells (WJMSCs) on SD rats with previous cesarean scar defect (PCSD). METHODS: SD rats (n=50) were randomly divided into 3 groups:10 in normal operation group (N-O group), 20 in model control group (M-C group) and 20 in model treatment group (M-T group). On the 19th day of pregnancy, thread was used for continuous suture after the caesarean section (C-S) in N-O group. After C-S, LPS (1 g/L, 1 mL/kg) was injected in uterine muscle walls and electrocoagulation (power=10 W) was used in bilateral uterine incision with interrupted suture in both M-C and M-T groups. The rats in M-T group was further divided into A and B subgroups, with 10 rats each. The rats in group A were treated by intravenous injection and vaginal perfusion, both with 0.5 mL of 1×109/L WJMSCs, while the rats in group B were treated by vaginal perfusion with 1 mL of WJMSCs, once a week, totally 3 times. The rats in M-C group were also divided into A and B subgroups (10 rats per group) and treated by the same way as M-T group, only except that saline was used instead of WJMSCs. At the 1st and 4th weeks after the treatment was finished, 6 and 4 rats were executed in every group, respectively. The uterine specimens with Masson and immunohistochemical staining were observed for determining the expression of actin and cytokeratin to reveal the repair of endometrial epithelium. RESULTS: The thickness of uterine muscle and volume density of actin in M-C group were significantly lower than those in N-O group, but the muscle collagen volume density and defect rate of endometrium were higher than those in N-O group. At the 1st and 4th weeks after treatment with WJMSCs, the muscular thickness and muscular actin concentration in M-T group (A and B) were significantly higher than those in M-C group, while the number of muscular collagen fibers were significantly lower than that in M-C group. The endometrial insufficiency in M-C group was higher than that in the other groups (P<0.05). CONCLUSION: WJMSCs promote the repair of the structure and function of myometrium in PCSD rats. No obvious difference between the effects of intravenous injection combined with vaginal perfusion, and vaginal perfusion alone on the repairing is observed. 相似文献
997.
以17%多福悬浮剂和20%福克悬浮剂2种种衣剂为供试材料,以辣椒为试验对象,进行温室大棚栽培试验,确认种衣剂使用的安全性,研究其对辣椒出苗、生长、果实品质及其根际土壤微生态的影响。结果表明:多福和福克对辣椒出苗和苗期生长均无显著影响,而多福影响辣椒现蕾期和开花期株高的生长,分别比对照减少4.60%和9.12%;多福减少开花期叶绿素含量,而福克无显著影响,多福和福克均显著影响辣椒根系活力,福克对根系活力的影响更为显著,在苗期、现蕾期和开花期分别比对照降低33.72%、78.05%和55.88%;多福和福克均降低辣椒品质相关指标中的硝态氮含量,分别比对照降低35.09%和27.94%;多福和福克不同程度影响辣椒根际土壤微生态,苗期和现蕾期对辣椒根际土壤脲酶、磷酸酶和过氧化氢酶活性的影响比较明显,多福和福克不同程度减少辣椒根际土壤细菌数量,而增加真菌数量;多福减少辣椒根际土壤速效磷的含量,而福克没有显著影响。 相似文献
998.
以分离自新疆古尔班通古特沙漠土壤的放线菌为试材,采用琼脂块法和生长速率法,研究了供试放线菌对草莓灰霉病菌的抑菌活性;采用形态学特征、生理生化特性、16SrDNA序列分析等方法对筛选出的高活性拮抗菌株进行种类鉴定。结果表明:从141株放线菌中筛选出1株对草莓灰霉病菌的抑菌圈直径为20.67mm的高活性拮抗菌株XJ93。该菌株无菌发酵滤液对多种植物病原真菌具有明显抑制作用,抑菌率为25.00%~71.67%;80℃处理60min其抑菌率未见明显变化,在pH 5~9范围内其抑菌活性稳定,在可见光和紫外线照射150min内亦保持良好的抑菌活性。拮抗菌株XJ93经鉴定为唐德链霉菌(Streptomyces tendae)。拮抗放线菌XJ93具有广谱抑菌活性,对环境有良好的稳定性,可作为防治果蔬草莓灰霉病的生物农药潜力菌株进行开发应用。 相似文献
999.
1000.