The extent of genetic diversity among Vanilla species: Comparative results for RAPD and ISSR     

Praveen C. Verma  Debasis Chakrabarty  Satya Narayan Jena  Devesh K. Mishra  Pradhyumna K. Singh  Samir V. Sawant  Rakesh Tuli 《Industrial Crops and Products》2009,29(2-3):581-589

Vanilla is a large genus of about 110 species in the orchid family (Orchidaceae), including the species Vanilla planifolia from which commercial vanilla flavoring is derived. Since most species of vanilla are considered rare and endangered there is an urgent need to conserve them through genetic analysis and propagation/conservation studies on this crop.The present study investigated the genetic diversity among nine leafy- and leaf-less Vanilla species employing 30 decamer RAPD primers and 10 ISSR primers. The species under study were diverse and displayed a range of variability (0–66% and 0–81% for RAPD and ISSR, respectively). A total of 154 RAPD polymorphic markers (83.24%, h = 0.378) and 93 ISSR polymorphic markers (86.11%, h = 0.363) were used to generate a genetic similarity matrix followed by the cluster analysis. Specific groupings were revealed by each cluster analysis with slight variation between two different markers. Among the nine species studied, V. planifolia, Vanilla aphylla and Vanilla tahitensis revealed very low level of variation within their collections, thus indicating a narrow genetic base. The large genetic distance of Vanilla andamanica from other species suggests its different origin. A close genetic affinity was observed between the pairs V. planifolia, V. tahitensis and Vanilla albida, V. aphylla. These are the first comparative results for RAPD and ISSR reporting inter-relationship among nine cultivated, wild and hybrid Vanilla species.  相似文献   

A NEW ESTROGENIC SUBSTANCE PRESENT IN THE NEUTRAL FRACTION OF HUMAN PREGNANCY URINE     

Dorfman RI 《Science (New York, N.Y.)》1940,92(2399):585-586

In addition to the well-defined phenolic estrogens in human pregnancy urine there appears to be another estrogenic substance which is a neutral non-ketonic compound.  相似文献   

SURFACE MOTION OF WATER INDUCED BY WIND     

Langmuir I 《Science (New York, N.Y.)》1938,87(2250):119-123

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The identification and characterization of a virus causing mosaic in Mertensia virginica     

NIENOW I 《Phytopathology》1948,38(1):62-69

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Experimentally developed telangiectasis and sawdust liver lesions in rats     

ROSENFELD I  BEATH OA 《Journal of the American Veterinary Medical Association》1948,112(854):386-389

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An outbreak of leptospirosis in cattle     

SUTHERLAND AK  MORRILL CC 《Journal of the American Veterinary Medical Association》1948,113(860):468-471

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Relationships between performance traits and the expression of growth hormone, insulin-like growth factor-I, and insulin in pigs selected for growth or leanness     

M. F. W. Te  Pas  C. L. M. Gerritsen  A. H. Visscher  K. H. de  Greef 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2003,120(5):346-357

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SYMPATHETIC MAGIC IN MODERN GUISES     

Dunlap K 《Science (New York, N.Y.)》1929,70(1824):566-568

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Harvest of Channel Catfish with an Electrically Enhanced Seine     

C. Douglas  Minchew  Kenneth K.  McDill Rachel V.  Beecham S. D. Filip To   《Journal of the World Aquaculture Society》2004,35(4):543-545

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Comparative antigenic analysis of extracellular proteins of Bacteroides nodosus isolated from virulent and benign ovine footrot     

W K Yong  L M Gordon 《Veterinary microbiology》1986,12(2):135-145

Antigens in the extracellular protein (ECP) complexes of Bacteroides nodosus, isolated from sheep with either benign or virulent footrot, were studied by immunoelectrophoresis (IEP). Rabbit antisera against ECP from virulent and benign strains, were used in homologous and heterologous crossed IEP. Four precipitin peaks unique to the virulent strain, and five peaks unique to the benign strain were identified. In an attempt to characterize the different antigens in ECP, rabbit antisera were raised against an outer membrane protein (OMP, mol. wt. 35 000 daltons), pili and various proteases of virulent and benign strains of B. nodosus. No precipitin band was observed when ECP from both B. nodosus strains were reacted against anti-OMP and anti-pilus antisera. However, single precipitin bands unique to one protease from the benign strain and one protease from the virulent strain were identified. The results suggest that specific antigens other than proteases or pili are important in determining whether a B. nodosus isolate is virulent or benign.  相似文献