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甜瓜果实中乙烯生产量及一些相关性状的遗传分析 总被引：1，自引：1，他引：0
为改进甜瓜货架期,利用成熟果实乙烯释放量及变化速率存在显著差异的3个甜瓜变种为亲本,配制了皇后×9-8(非跃变类型材料间)和皇后×Charentais(非跃变类型与跃变类型材料间)两个组合,对各组合F1,F2及回交世代BC果实中乙烯的生成变化及果实脱落性、外果皮迅速黄化等相关性状的遗传变化进行了分析。结果表明,甜瓜果实中乙烯生成量为不完全显性遗传,受两对以上基因控制。在皇后×Charentais组合的F1中,内源乙烯含量在采收1d后表现出急剧升高,同时伴随着外果皮迅速黄化及果柄脱落的发生,根据这些性状在F2及分别与父母本回交世代中的不同分离比率指出,乙烯的跃变类型、外果皮迅速黄化及果柄脱落在甜瓜果实中为显性遗传。 相似文献
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SIQIN Mari NAKAI Tomohiro HAGI Shinichi KATO Ali Mohammed PITIA Mai KOTANI Yuki ODANAKA Yasushi SUGAWARA Koh‐Ichi HAMANO Keiichiro YOGO Yoshio NAGURA Masaru FUJITA Hiroshi SASADA Eimei SATO Tetsuya KOHSAKA 《Animal Science Journal》2010,81(6):681-686
Relaxin‐like factor (RLF), also known as insulin‐like factor 3 (INSL3), is produced by testicular Leydig cells, but its specific receptor LGR8 (leucine‐rich repeat family of G‐protein‐coupled receptor 8) has not been identified in goats. This study aimed to identify complementary DNA (cDNA) sequences of goat LGR8, and characterize the expression of both RLF and LGR8 in goat testes by RT‐PCR and immunohistochemistry. Testes were collected from immature (3‐month‐old) and mature (24‐month‐old) Saanen goats, and partial cDNA sequences of the goat homologue of human LGR8 were identified. The sequence encoded a reduced peptide sequence of 167 amino acids, which corresponded to transmembrane regions 2 through 5, followed by the beginning of intracellular loop 3 of human LGR8. Expression of both LGR8 and RLF genes was drastically increased in mature testes compared with immature ones. Although RLF protein was restricted to Leydig cells, LGR8 protein was detected in both Leydig cells and seminiferous epithelial cells (possibly germ cells and Sertoli cells). These results reveal a possible existence of the RLF‐LGR8 ligand‐receptor system within the goat testis, suggesting that RLF may play a role in testicular function through LGR8 on Leydig cells and seminiferous epithelial cells in an autocrine and/or paracrine manner. 相似文献
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Ayano SATO Toshihide KATO Motoshi TAJIMA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(6):940
Flexural and hyperextension deformities are congenital problems in calves. We, therefore, aimed to investigate the distal limb conformation in 1 day- and 28-day-old female Holstein-Friesian (HF) calves (n=21), male Japanese Black (JB) calves (n=15), and female JB calves (n=15). The claw angle of the forelimb dorsal claw wall in a standing position and recorded other parameters, including body weight, withers height, circumference of forelimbs, and flexor tendon thickness in the forelimbs, were measured and compared these between the three groups. At 1 day old, the mean claw angles were 51.1° in female HF calves, 47.0° in male JB calves, and 41.8° in female JB calves; the 95% confidence intervals (CIs) of the claw angles showed large distributions in all three groups. One female HF and one male JB calves showed mild flexural deformity, whereas four JB calves showed hyperextension deformity. At 28 days old, the mean claw angles were 51.7° in female HF calves, 51.2° in male JB calves, and 48.4° in female JB calves; the 95% CIs of the claw angles showed smaller distributions than those at 1 day old in all groups. For all groups, the limb deformities had improved without treatment at 28 days old. As a feature of the breed, female JB calves were apt to show hyperextended deformities inversely proportional to the body weight. These limb deformities healed spontaneously and were thought to be physiological. 相似文献
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ABSTRACT: Heating temperatures of 30–40°C and KCl concentrations of 0.1–0.5 M altered the denaturation mode of carp myofibrils. In 0.1 M KCl medium, heating temperature affected the denaturation of rod more significantly than of subfragment-1 (S-1), and a slow decrease in solubility at 30°C was accompanied by a slow denaturation of rod. KCl concentration at heating altered the denaturation mode differently at 30°C and 40°C. Increased KCl concentrations for heating reduced the rod denaturation rate at 40°C, but it was increased at 30°C. At concentrations above 0.3*Τ*M KCl, the denaturation rate for rod became identical to that for S-1 at both temperatures. Upon heating of chymotryptic digest of myofibrils, S-1 denaturation was similarly detected as in intact myofibrils, whereas practically no rod denaturation was detected. Thus, it was concluded that myosin structure connecting S-1 and rod has an important role in the denaturation process. 相似文献
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ABSTRACT: We examined the stomach contents of 26 Baird's beaked whales caught off the coast of Japan by small-type coastal whalers. The main prey for these whales was rat-tails and hakes in the western North Pacific. Pollock and squids were also important food in the whales collected from the southern Sea of Okhotsk. The prey species found in the stomachs of the whales were almost identical to those caught in bottom-trawl nets at depths greater than about 1000 m in the western North Pacific, which suggests that the Baird's beaked whale forages for prey at depths of about 1000 m or more. Baird's beaked whales in the western North Pacific migrate to waters of 1000–3000 m in depth, where demersal fish are abundant. This implies that Baird's beaked whales migrate to waters where demersal fish, especially rat-tails and hakes, are abundant. Although there is limited information on the feeding habits of ziphiid whales, they are generally thought to prefer squid. The present data suggest that demersal fish are also important prey for ziphiid whales. 相似文献
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ABSTRACT: Sequence variability of the control region of mitochondrial DNA in five Sebastes species ( S. thompsoni , S. joyneri , S. inermis , S. schlegeli and S. owstoni ) was investigated. Of 324 nucleotide sites in the control region of S. thompsoni , 56 sites (17.3%) varied, and all 20 individuals had different haplotypes. The other four species had between five and 17 sites (1.5–5.9%), which was fewer than that observed in S. thompsoni . The nucleotide diversity of S. thompsoni was highest (3.45%) among the five species, and that of S. schlegeli was the lowest (0.19%). The results demonstrated that sequence variability exists in the control region of the five Sebastes fishes investigated, and that sequence data in the control region may be suitable for stock structure analysis. Also, the extent of genetic variablitiy in the control region differed among these species. In particular, the mitochondrial DNA control region in S. thompsoni was characterized by high sequence variability, which may indicate a large effective population size. 相似文献
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