FAD-dependent sulfhydryl oxidase activity of augmenter of liver regeneration protein and its activity motif     

PAN Yan  JU Gui-zhi  TONG Ming-hua  LI Ru-bing  YANG Lian-ping  LI Xiu-yi  KONG Xiang-ping 《园艺学报》2006,22(11):2247-2250

AIM:To establish a sensitive and effective method to determine whether augmenter of liver regeneration protein (ALRp) has the activity of sulfhydryl oxidase.METHODS:The cysteine at the position of 65 in CXXC motif contained in ALRp was changed into alanine through site-directed mutation.There were four groups in the experiment:① ALR group;② ALR-FAD group;③Mutated ALR-FAD group;④ Control group.Using GSH as substrate,at different time points,the concentration of thiol in each group was measured.RESULTS:The concentration of thiol groups in the ALR-FAD group was decreased during the time of the experiment.But the thiol in the other groups didn’t show any significant decrease.CONCLUSION:A sensitive and effective method to determine the sulfhydryl oxidase activity of ALRp was established.ALRp is a FAD-dependent sulfhydryl oxidase,and the conserved CXXC motif is indispensable to ALRp.  相似文献   

Proliferation and apoptosis of neuroendocrine cells in ovarian epithelial tumors     

JIANG Li-yan  WANG Zi-neng  ZHONG Xue-yun  ZHENG Pei-er  LI Hong  LUO Xin  XU Jian-ping  WANG Xiao-yu 《园艺学报》2006,22(5):856-859

AIM: The purpose of this study was to observe the morphological features of neuroendocrine cells (NECs)， their proliferation and apoptosis in ovarian epithelial tumors, and to discuss their biological and clinical significance. METHODS: 79 specimens of ovarian epithelial tumor samples were collected, of them 20 benign, 18 boderline, 41 milignant tumors, and 22 normal ovaries were investigated immunohistochemically. Chromogranin A was used to detect NECs and their proliferation and apoptosis were examined by double-label staining of chromogranin A and Ki67 or TUNEL. RESULTS: The positive rate of CgA, distribution and staining intensity in ovarian epithelial tumors were higher than those in normal ovary. NECs showed various shapes with neuronoid protuberances stretching to the neighboring cells or basement membrane. Occasionally, they might touch together. No TUNEL positive coexpression in all NECs was observed by double-label staining, but some NECs were coexpressed with Ki67. CONCLUSION: NECs of ovarian epithelial tumors like cancer cells showed a proliferation, but no apoptosis. Their secretion might promote their neighboring non-NECs to proliferate and prevent them from apoptosis.  相似文献   

Expression of telomerase inhibitor Pinx1 in leukemia cells and its correlation with telomerase activity     

SUN Jie  TAN Ya-min  HUANG He  ZHU Yuan-yuan  LAN Jian-ping  LAI Xiao-yu 《园艺学报》2006,22(9):1725-1728

AIM:To study the expression of telomerase inhibitor Pinx1 in acute leukemia cells and during the differentiation of acute promyelocytic leukemia cells,and to realize its effect on telomerase activity.METHODS:Realtime quantitative PCR with fluorescence probe hybridization was used to measure the expression of Pinx1 and hTERT mRNA in acute leukemia cells and during differentiation of NB4 cells induced by ATRA.The correlations between Pinx1 and hTERT expression were also analyzed.RESULTS:Pinx1 mRNA expression in acute leukemia samples (0.00312,5.42×10-4-0.024) was significantly higher than that in normal bone marrow mononuclear cells (7.89×10-4,0-0.00863,P<0.01).The expression of Pinx1 mRNA had significant positive correlation with hTERT mRNA expression (r=0.296,P<0.05).Pinx1 mRNA expression decreased during differentiation,its expression was positive correlated with hTERT mRNA expression (r=0.900,P<0.05).CONCLUSIONS:As an inhibitor of telomerase,however,Pinx1 also had the same direction of regulation with telomerase activity in acute leukemia cells,suggesting its expression variation may be a subsequent reaction induced by that of hTERT to stabilize telomerase activity.The exact mechanisms remained to be verified.  相似文献   

杏(Prunus armeniaca)自交不亲和强度及其授粉受精相关特性   总被引：6，自引：0，他引：6  

冯建荣  陈学森  孔宁  束怀瑞 《果树学报》2006,23(5):690-695

为了解杏自交不亲和性强度与授粉受精相关特性的关系,以自交亲和(Self-compatibility:SC)品种凯特(Prunus armeniaca L．cv．Katy)和自交不亲和(Self-incompatibility:SI)品种新世纪(P.armeniaca L.cv．Xinshiji)及凯特×新世纪杂种群体为试材,荧光显微镜观察自交亲和与自交不亲和杏花粉管生长动态。结果表明,授粉后初期,自交亲和性与自交不亲和性的杏花粉都能正常萌发、生长,但是在花粉管生长延伸到花柱1／2以后,自交亲和性的花粉管能顺利进入子房,而自交不亲和性的花粉管多数顶端膨大呈球形,停止向下生长,只有极个别能正常生长到达子房;杂种后代的可溶性蛋白含量和RNA酶比活力,与亲本相比无明显的趋中变异表现,而且在自交亲和与自交不亲和杏之间无显著性差异。  相似文献   

实时荧光定量PCR检测PrP基因在金黄地鼠脑组织的动态表达     

宁章勇  赵德明  杨建民  周向梅  于博  孔小明  尹晓敏 《中国兽医杂志》2007,43(9):3-6

金黄地鼠是研究动物传染性海绵状脑病的理想模型动物之一,其脑组织内朊蛋白基因动态表达数据的测定对探讨该类疾病的发生、发展和分子致病机理具有重要意义。我们利用实时荧光定量RT-PCR技术,对不同年龄金黄地鼠大脑、小脑、丘脑和脑干PrP基因的表达进行了定量。结果发现,脑的四个检测部位都呈现高的表达量,但是同一年龄段不同组织每纳克总RNA中朊蛋白基因的表达量和每毫克组织中朊蛋白基因的表达量有显著的差别,不同组织在不同年龄出现表达高峰。本研究的结果对于探讨朊蛋白的基本功能和脑组织在传染性海绵状脑病病理发生中的作用,提供了基础数据。  相似文献   

应用PCR检测鸭肠炎病毒弱毒在鸡胚体内的分布   总被引：2，自引：0，他引：2  

陈淑红  刘怀然  韩宗玺  邵昱昊  刘胜旺  孔宪刚 《中国预防兽医学报》2007,29(1):17-21

根据GenBank已发表的鸭肠炎病毒（DEV）UL6基因的部分核苷酸序列，设计并合成一对引物．以鸭肠炎病毒中国商品化疫苗毒蚀斑纯化株（DEV Clone-03）DNA为模板，经PCR扩增出预期516bp的目的片段。将此片段插入克隆载体质粒pMD18-T，并进行序列测定，序列比较结果显示，此段序列与参考序列完全一致。用PCR检测DEV Clone-03在鸡胚体内的分布情况显示，接毒死亡鸡胚的尿囊膜、心、肝、脾、肺、肾和肌肉皮肤内均存在病毒。PCR敏感试验表明此方法可以检测到100μL尿囊液中提取的10^-3稀释的病毒DNA，相当于0．2个EID50病毒量的DNA。  相似文献   

口蹄疫病毒WFL株基因组全长cDNA的克隆及序列分析   总被引：1，自引：0，他引：1  

任林柱  王兴龙  李莉  李晓艳  段小宇  梅英武  刘锴  王学理 《中国预防兽医学报》2007,29(1):22-26

根据口蹄疫病毒基因组的结构特点以及GenBank上公布的全序列，用DNAMAN分别设计了涵盖整个基因组序列的3对引物，从接种口蹄疫病毒WFL株的细胞培养液中提取了病毒基因组RNA，采用RT-PCR方法和RACE法分别扩增了3条基因片段，并将扩增片段分别与T载体连接，在体外分别进行了5-半分子和3’半分子的构建。最后将5’半分子和3’半分子连接成基因组全长cDNA分子。经PCR鉴定、酶切鉴定及全长cDNA测序，证实成功构建了口蹄疫病毒wFL株基因组全长eDNA分子。序列分析结果表明，供试口蹄疫病毒基因组全长为8155nt，5＇UTR长1059nt；具有一个大的读码框，其核苷酸长度为6969nt。包括201aa的前导蛋白基因和2122aa的聚合蛋白基因；3＇UTR长127nt，包括34nt的polv（A）。  相似文献   

黑木耳胞外酶活变化与栽培性状比较的研究   总被引：1，自引：1，他引：1  

韩增华  张丕奇  孔祥辉  马庆芳  戴肖东  张介驰 《食用菌学报》2007,14(4):41-46

研究了黑木耳10个菌株胞外酶活性及与栽培性状和产量的关系,结果表明,胞外漆酶、多酚氧化酶活性的变化趋势大致相似,酶活与栽培产量呈正相关,相关系数为0.823(7号菌株除外),酶活性相对较高的菌株(3、4、8号),栽培中产量相对较高。纤维素酶活性高峰时间出现较漆酶、多酚氧化酶早,且酶活性高峰出现早的菌株菌丝长速较快。蛋白酶活与栽培产量呈一定负相关性,相关系数为-0.700(6号菌株除外),蛋白酶活最低的3号菌株,栽培产量最高;而活性较高的5、9号菌株栽培产量较低。  相似文献   

叶面喷施硼酸对苹果果实硼和钙含量的影响   总被引：2，自引：0，他引：2  

魏宗梅  许雪峰  李天忠  王忆  孔瑾  韩振海 《园艺学报》2007,34(5):1111-1116

 以‘富士’苹果为试材，通过幼果期、果实膨大期和果实成熟期叶面喷施硼酸，研究硼对果实硼和钙含量的影响。结果表明：从叶面喷硼对果实吸收硼的短期效果来看，果实发育的不同时期叶面喷施硼酸均促进果实对硼、钙的吸收，并且处理的效果顺序是：幼果期>果实膨大期>果实成熟期；在果实采收时硼含量为：果实膨大期>果实成熟期>幼果期。不同时期叶面喷施硼酸均提高了果肉细胞中水溶态硼、半束缚态硼和束缚态硼的绝对含量，从3种形态硼所占的比例来看，随着果实不断发育，水溶态硼和半束缚态硼比例升高，而束缚态硼比例下降。  相似文献   

Renoprotective effects of sesamin on renal hypertensive and hyperlipidemic rats     

XU Chao-yang  YANG Jie-ren  GUO Li-qun  KONG Xiang 《园艺学报》2007,23(5):913-915

AIM: To investigate the effects of sesamin on progression of renal injury in renal hypertensive and hyperlipidemic rats (RHHR). METHODS: RHHR was induced by 2K1C and high lipid baitvessel. After 7 weeks of intragastric administration with sesamin, the contents of serum creatinine (Scr), blood urea nitrogen (BUN), 24 h urinary protein excretion (UPE) were measured. In addition, the activity of total antioxidative capacity (T-AOC), superoxide dismutase (SOD), the concentrations of malondialdehyde (MDA), hydrogen peroxide (H2O2), and the nitric oxide synthase (NOS) and nitric oxide (NO) levels in renal homogenate were measured. RESULTS: Compared with the model group, seasamin (in 100 mg·kg-1 and 33 mg·kg-1 groups) evidently decreased the contents of Scr, BUN, UP and the concentration of MDA, iNOS, H2O2 in renal tissure. It also improved the levels of NO, cNOS and activity of SOD, T-AOC in renal tissure. CONCLUSION: Sesamin ameliorates hypertensive and hyperlipidemic-induced renal injury, probably by enhancing antioxidative activity, scavenging hydroxyl radical and restraining iNOS level.  相似文献