钙对枳生长发育及柑橘溃疡病抗性的影响     

肖桂华  文康  韩健  郝晨星  叶蓉春  朱亦赤  萧顺元  邓子牛  马先锋 《中国农业科学》2022,55(19):3767-3778

【背景】柑橘溃疡病是由柑橘黄单胞杆菌柑橘致病变种(Xanthomonas citri subsp. citri,Xcc)引起的细菌性病害,可侵染枝、叶、果,危害几乎所有的柑橘主栽品种。前期对湖南省39个柑橘园的调查结果显示柑橘果园土壤酸化和交换性钙缺乏严重,叶片中均存在钙缺乏现象。钙是植物所需大量元素之一,钙缺乏会造成植物营养失衡,生长势下降,植物免疫水平受影响。然而,钙元素对柑橘溃疡病抗性的影响尚不明确。【目的】分析对柑橘溃疡病敏感的枳(Poncirus trifoliata)在不同钙浓度处理下叶片注射接种Xcc后的致病差异,探讨钙在Xcc侵染枳叶片过程中的作用。【方法】采用沙培法对枳实生苗进行0、0.75、3、30 mmol·L^-1钙浓度处理,分别测定枳生长期的生物量、叶绿素a和b浓度、根系和叶片钙元素含量、观察根系活性氧(ROS)的产生和胼胝质沉积,并分析枳叶片接种Xcc后细胞壁合成相关基因及免疫途径相关基因诱导表达变化特征。【结果】以3 mmol·L^-1钙处理为对照,0、0.75和30 mmol·L^-1钙处理后,...  相似文献   

〗Effects of PPAR-α silence on ET-1 induced myocardial hypertrophy and PI3K/Akt/GSK3β-NFATc4 signal pathway     

LI Rui-fang  LE Kang  GAO Jie  YANG Guo-qing  BAO Ying-xia  LIU Pei-qing 《园艺学报》2009,25(12):2289-2294

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Variability in energy partitioning and resistance parameters for a vineyard in northwest China     

Sien Li  Fusheng Li  Shaozhong Kang 《Agricultural Water Management》2009,96(6):955-962

Grapevines are extensively grown in the semiarid and arid regions, but little information is available on the variability of energy partitioning and resistance parameters for the vineyard. To address this question, an eddy covariance system was applied to measure energy balance over a vineyard in northwest China during 2005-2006. Result indicated that 2-year average Bowen ratio (β) of vineyard was 1.0, canopy resistance (r_c) 289.3 s m⁻¹, aerodynamic resistance (r_a) 9.7 s m⁻¹ and climatological resistance (r_i) 117 s m⁻¹. This implied that the annual energy was split almost equally between sensible heat and latent heat. Compared to the corresponding values in other ecosystems reported by Wilson et al. [Wilson, K.B., Baldocchi, D.D., Aubinet, M., Berbigier, P., Bernhofer, C., Dolman, H., Falge, E., Field, C., Goldstein, A., Granier, A., Grelle, A., Halldor, T., Hollinger, D., Katul, G., Law, B.E., Lindroth, A., Meyers, T., Moncrieff, J., Monson, R., Oechel, W., Tenhunen, J., Valentini, R., Verma, S., Vesala, T., Wofsy, S., 2002. Energy partitioning between latent and sensible heat flux during the warm season at FLUXNET sites. Water Resource Research 38, 1294-1305.], the vineyard had a higher β, r_c and r_i than deciduous forests, corn and soybean, and grassland. Such difference was mainly attributed to (1) serious water stress in 2005, which resulted in a greater r_c up to 364.4 s m⁻¹; (2) sparse canopy with row spacing of 2.9 m and plant spacing of 1.8 m; (3) warm-dry climate and high attitude (1581 m) along with higher r_i and lower psychrometer (54 Pa K⁻¹) in the arid region of northwest China. These characters of vineyard revealed varying process of energy partitioning and surface resistance, and provided a scientific basis in understanding and modeling water and energy balance for the vineyard in the semiarid and arid regions.  相似文献   

Relationship between stable carbon isotope discrimination and water use efficiency under regulated deficit irrigation of pear-jujube tree   总被引：2，自引：0，他引：2  

Ningbo Cui  Taisheng Du  Shaozhong Kang  Fusheng Li  Xiaotao Hu  Mixia Wang  Zhijun Li 《Agricultural Water Management》2009,96(11):1615-1622

To investigate the relationship between stable carbon isotope discrimination (Δ) of different organs and water use efficiency (WUE) under different water deficit levels, severe, moderate and low water deficit levels were treated at bud burst to leafing, flowering to fruit set, fruit growth and fruit maturation stages of field grown pear-jujube tree, and leaf stable carbon isotope discrimination (Δ_L) at different growth stages and fruit stable carbon isotope discrimination (Δ_F) at fruit maturation stage were measured. The results indicated that water deficit had significant effect on Δ_L at different growth stages and Δ_F at fruit maturation stage. As compared with full irrigation, the average Δ_L at different growth stages and Δ_F at fruit maturation stage were decreased by 1.23% and 2.67% for different water deficit levels, respectively. Δ_L and Δ_F among different water deficit treatments had significant difference at the same growth stage (P < 0.05). Under different water deficit conditions, significant relationships between the Δ_L and WUE_i (photosynthesis rate/transpiration rate, P_n/T_r), WUE_n (photosynthesis rate/stomatal conductance of CO_2, P_n/g_s), WUE_y (yield/crop water consumption, Y/ET_c) and yield, or between the Δ_F and WUE_y and yield were found, respectively. There were significantly negative correlations of Δ_L with WUE_i, WUE_n, WUE_y and yield (P < 0.01) at the fruit maturation stage, or Δ_L with WUE_i and WUE_n (P < 0.01) over whole growth stage, respectively. Δ_F was negatively correlated with WUE_y, WUE_n and yield (P < 0.05), but positively correlated with ET_c (P < 0.01) over the whole growth stage. Thus Δ_L or Δ_F can compare WUE_n and WUE_y, so the stable carbon isotope discrimination method can be applied to evaluate the water use efficiency of pear-jujube tree under the regulated deficit irrigation.  相似文献   

金属板材数控渐进成形螺旋线轨迹生成   总被引：2，自引：1，他引：1  

朱虎  扶建辉  姜在宽 《农业机械学报》2009,40(10):223-226

在分析金属板材数控渐进成形特点的基础上,指出现行等高线运动成形方式存在的问题,提出了一种基于螺旋运动的数控渐进成形方式和基于STL数据模型的螺旋线成形加工轨迹的生成算法。利用顶点偏置方法生成STL模型的等距模型,采用一系列水平面和与Z轴平行的平面分别切割该等距模型,求出其交线的交点,并将其连接成螺旋线成形加工轨迹。算法应用实例表明,该算法能够生成无干涉的螺旋线成形加工轨迹。  相似文献   

提辊式拔棉秆阻力分析   总被引：1，自引：0，他引：1  

郭振华  史建新  康秀生 《农机化研究》2009,31(2)

机械化拔棉秆对提高农业生产效率有重要意义.以提辊式拔棉秆机械的工作部件为研究对象,并对提辊建立数学模型,分析其受力和各项功耗,得出各项阻力在总阻力中所占的比例关系;根据各参数与总阻力之间的数学关系建立函数图像,分析得出影响牵引阻力的主要参数与阻力之间的关系;结合试验,进一步得到提辊参数间的优化组合,为提辊式拔棉秆机械的进一步优化设计提供参考.  相似文献   

直线共轭内啮合齿轮泵齿轮接触强度的有限元分析     

袁丹青  康仕彬  丛小青  刘梦仙 《排灌机械》2009,27(2):111-114

针对采用传统经验公式分析计算齿轮泵齿轮接触强度存在计算过程相当复杂、繁琐,误差较大的问题.在分析齿轮受力情况基础上,采用有限元法对直线共轭内啮合齿轮泵齿轮接触强度进行计算,并与经验公式计算的接触强度进行比较分析.结果表明,两种方法计算结果基本吻合,证实了采用有限元法分析计算的可行性与优越性,为直线共轭内啮合齿轮泵齿轮的设计、选材提供了一种新的方法,具有一定的工程应用价值.  相似文献   

Compensatory growth and body composition of juvenile black rockfish <Emphasis Type="Italic">Sebastes schlegeli</Emphasis> following feed deprivation   总被引：1，自引：0，他引：1  

Sung-Yong Oh  Choong Hwan Noh  Rae-Seon Kang  Chong-Kwan Kim  Sung Hwoan Cho  Jae-Yoon Jo 《Fisheries Science》2008,74(4):846-852

ABSTRACT:   Compensatory growth, feeding rate, feed efficiency and chemical composition of juvenile black rockfish (mean weight 1.43 g) were investigated for 35 days after a 14-day feed deprivation treatment under four feeding conditions: one group continuously fed (control) and the other three groups fasted for 5 days (F5), 10 days (F10) and 14 days (F14). All fasted fish were re-fed from day 15. Only F5 achieved the same body weight as the control, indicating that complete compensation occurred in F5. The specific growth rate (SGR) of F5 was the highest at day 21 and then decreased thereafter, showing higher values than the control at days 21, 28 and 42. In contrast, although SGRs of F10 and F14 were higher than that of the control during the whole refeeding period except day 21, they did not catch up the control in body mass, indicating that only partial compensation occurred in F10 and F14. The feeding rate (FR) of all groups except F14 changed in a pattern similar to SGR (Spearman's rank correlation, r _s > 0.9), suggesting that SGR varied depending on FR. Similar feeding efficiencies (FEs) were found in the four groups and they did not vary significantly during the whole refeeding period, suggesting that FE was not the factor affecting SGR. At day 14, the ratios of lipid to lean body mass in F10 and F14 were lower than those in the control and F5, and there was no difference between the control and F5. At day 49, however, only F14 showed a lower value than the other three groups, and there was no difference among the three groups. These results indicate that juvenile black rockfish fasted for 5–14 days can exhibit compensatory growth after refeeding, but timing and degree vary depending on the duration of feed deprivation.  相似文献   

The activation effect of maize pollen polysaccharides on human thoracic cavity macrophages     

JIN Li-qin  LU Jian-xin  YU Kang  YUAN Qian  XIE Ke-jian  WANG Kai-fa  ZHANG Yu-lan 《园艺学报》2000,16(8):726-729

AIM: To study the activation effects of maize pollen polysaccharides(PPM) on human thoracic cavity macrophage (hTMΦ). METHODS: Activities of lactate dehydrogenase(LDH) and acid phosphatase (ACP) in the hTMΦ were detected by automatic biochemical analyzer, and the level of tumor necrosis factor alpha(TNF-α) and interleukin-6(IL-6) in the hTMΦ was analyzed by radioimmunoassay, after hTMΦ were cultured with 0.312, 0.625, 1.250, 2.500, 5.000 mg/mL PPM-RPMI 1640 for 24 and 48 hours in vitro. RESULTS: The activities of LDH and ACP increased in the hTMΦ induced by PPM, and the levels of TNF-α and IL-6 in the hTMΦ induced by PPM increased markedly too. And the induced expression effect of TNF-α and IL-6 is associated with the concentration of PPM, and time for PPM inducing. CONCLUSION: PPM can induce cytokines secretion in hTMΦ, and activate hTMΦ in vitro.  相似文献   

性控奶山羊精液X和Y精子分离比例检测方法的建立     

何琪富  王宇飞  张康  康健  张涌  权富生 《畜牧兽医学报》2021,52(1):107-115

旨在建立可以定量计算奶山羊精液中X、Y精子数量的双重TaqMan荧光定量PCR方法,用以检测经过分离的奶山羊精液X和Y精子的数量和比例,为性控技术的开发和生产应用提供技术支撑.本研究选择X、Y染色体中特异基因F9及ZFY片段设计引物,建立标准曲线,优化荧光定量PCR反应体系和条件.通过对阳性标准品梯度稀释以及对60支已...  相似文献