Effect of cytolethal distending toxin of Campylobacter jejuni on adhesion and internalization in cultured cells and in colonization of the chicken gut     

Biswas D  Fernando U  Reiman C  Willson P  Potter A  Allan B 《Avian diseases》2006,50(4):586-593

Campylobacter jejuni produces cytolethal distending toxin (CDT) that causes host cells to arrest during their cell cycle and that is involved in the pathogenesis of inflammatory diarrhea in humans. To assess the role of CDT in adherence and invasion of different cultured host cells (HeLa and HD-11) and in colonization of the chicken intestine, the genes of C jejuni NCTC11168 encoding the toxin subunits (cdtA, cdtB, and cdtC) were inactivated by insertional mutagenesis. No significant difference was found in adhesion of the wild-type C. jejuni and the isogenic mutants to HeLa and HD-11 cells. All of the mutants exhibited a decrease (>10-fold) in the ability to invade HeLa cells, but no significant difference was noticed for HD-11 cells. The ability of mutants to colonize birds either directly or by horizontal transfer was unchanged. These data indicated that although the production of cytotoxin does not play a role in the adherence to either human or avian cells, it may play a role in the invasion, survival, or both of C. jejuni in human cells, which are more susceptible to C. jejuni internalization. The CDT also does not seem to play a role in the colonization of poultry.  相似文献   

Nitrogen transformation and carbon sequestration in wetland paddy field of Bangladesh     

Alam  Md. Ashraful  Rahman  Md. Mizanur  Biswas  Jatish Chandra  Akhter  Sohela  Maniruzzaman  M.  Choudhury  Apurba Kanti  Jahan  M. Abu Hena Sorwar  Miah  Md. Main Uddin  Sen  Ranjit  Kamal  M. Zia Uddin  Mannan  Md. Abdul  Shiragi  Humayun Kabir  Kabir  Wais  Kalra  Naveen 《Paddy and Water Environment》2019,17(4):677-688

Paddy and Water Environment - Rice-based cropping systems in Bangladesh have received little consideration of changing nitrogen (N) and carbon (C) in soil. Therefore, this study was conducted at...  相似文献   

Citric acid loaded nano clay polymer composite for solubilization of Indian rock phosphates: a step towards sustainable and phosphorus secure future     

Trisha Roy  Samar Chandra Datta  Abhijit Sarkar  Siddhartha Sankar Biswas 《Archives of Agronomy and Soil Science》2018,64(11):1564-1581

Non-renewable nature of rock phosphate (RP) reserves coupled with open ended nature of P cycle makes it imperative for maximum utilization of available P resources. In this context, use of Indian RPs from Purulia and Udaipur along with citric acid loaded nanoclay polymer composite (CA-NCPC) as P source to costly diammonium phosphate (DAP) was investigated through an incubation experiment followed by a greenhouse experiment with wheat-rice cropping sequence in a Luvisol (pH 5.14, available P 13.5 mg kg^?1). Soil available P, crop yield parameters and dynamics of soil P fractions were taken to judge the efficacy of CA-NCPC in solubilizing RPs. Application of CA-NCPC and DAP resulted in 82% and 69% increase in available P over control, respectively under incubation study. Direct effect of treatment receiving CA-NCPC + RP on yield and P uptake by wheat was comparable with DAP but residual impact of CA-NCPC + RP (16.7 g pot^?1) was better than DAP (13.8 g pot^?1) in rice. The changes in inorganic P fractions were also significant as inclusion of RP increased calcium-P from 16.1 to 61.5 mg kg^?1. Results indicated potentiality of RPs treated with CA-NCPC as an alternate P source which could prove promising amidst P scarcity.  相似文献   

A Comparison of Diffusion-Conductimetric and Distillation-Titration Methods in Analyzing Ammonium- and Nitrate-Nitrogen in the KCl-Extracts of Georgia Soils     

Uttam K. Saha  Leticia Sonon  Bipul K. Biswas 《Communications in Soil Science and Plant Analysis》2018,49(1):63-75

Soil mineral (or inorganic) nitrogen (SMN), which primarily exists as exchangeable and soluble ammonium (NH4⁺) and the nitrate (NO₃^?) ions, represents readily available nitrogen for plant growth. Over the years a 2M potassium chloride (KCl) solution has become the extraction solution of choice for extracting SMN. In the research and service laboratories, either distillation-titration method (DTM) or colorimetric method (CM) is virtually the standard to measure NH₄⁺- and NO₃^?-N in the 2M KCl soil extracts. However, being a time-consuming and labor intensive method, DTM generally has a very low throughput. Likewise, CM is affected by interferences from pH variation, color, turbidity, presence of organic species, and some other constituents in the extracts. In contrast, diffusion conductivity method (DCM) is a less expensive and high throughput one, which is also relatively free from common interferences. In this study, we, therefore, compared the extraction efficiency of various KCl concentrations and performance of diffusion conductivity method (DCM) with DTM in measuring NH₄⁺-N and NO₃^?-N in KCl extracts of 32 agricultural soils of Georgia. A 0.2M KCl solution extracted statistically similar amounts of NH₄⁺-N and NO₃^?-N as did 2M KCl, suggesting that a 10-fold dilute KCl solution than the standard 2M KCl might be good enough to extract and estimate the most of SMN. For the analyses of NH₄⁺- and NO₃^?-N in the KCl extracts, the DCM produced results statistically similar to those produced by DTM. The deviation between the results given by DCM and DTM was no more than ±10%. Thus, DCM appears to be an attractive alternative to the labor intensive and time-consuming DTM for measuring NH₄⁺- and NO₃^?-N in the KCl extract of soils in the research and service laboratories.  相似文献   

Nutrient transformations in soil amended with rock phosphate enriched composts for improving productivity of wheat-green gram sequence     

P. C. Moharana  D. R. Biswas 《Journal of plant nutrition》2017,40(10):1485-1506

The present study focuses on the transformations of nitrogen (N), phosphorus (P), and sulfur (S) in soil amended with rock phosphate (RP) enriched composts prepared using different crop residues under wheat-green gram sequence. Data emanated from the field study revealed that significant buildup in available pools of potassium permanganate (KMnO₄-N), Olsen-P, and calcium chloride (CaCl₂-S) as well as different fractions of N, P, and S were maintained higher under RP enriched composts treated plot than control. The total N, P, and S varied from 979 to 1776, 604 to 1165, and 273 to 464 kg ha^?1, respectively in different treatments. The yield of wheat and green gram was significantly correlated with different fractions of N, P, and S in soil. Results conclude that RP enriched compost could be an alternative and cost-effective option for mitigating the shortage of chemical fertilizers for crop production.  相似文献   

Morphological identification of foodborne pathogens colonizing rice grains in south Asia     

Reddy KR  Farhana NI  Wardah AR  Salleh B 《Pakistan journal of biological sciences: PJBS》2010,13(16):794-801

The aim of this study was to identify the foodborne pathogens mainly, Aspergillus sp. colonizing rice grains using cultural and microscopic methods. Four differential media (Czapek Dox Agar (CZA), Czapek Yeast Agar (CYA), Malt Extract Agar (MEA) and Czapek yeast 20% sucrose agar (CYA20S)) were used for differentiation of five Aspergillus sp., colonizing rice grains comparing with standard cultures. We studied macroscopic (colony color and diameter, conidia color, exudates, sclerotia and colony texture) and microscopic (conidiophore color, length and breadth, conidia size, shape and surface texture, vesicle diameter and phialides length and breadth) characteristics for identification of 110 isolates of Aspergillus sp. isolated from 65 rice grain samples collected from various countries in South Asia (Cambodia, India, Indonesia, Malaysia and Thailand). According to morphological characters, all these isolates were belonging to Aspergillus flavus (45), A. fumigatus (8), A. ochraceus (7), A. niger (42) and A. tamarii (8). This is the first report on identification of large number of Aspergillus strains isolated from rice grains in South Asia.  相似文献   

Azide derivatives of soybean oil and fatty esters     

Biswas A  Sharma BK  Willett JL  Advaryu A  Erhan SZ  Cheng HN 《Journal of agricultural and food chemistry》2008,56(14):5611-5616

An environmentally friendly water-based pathway to form the azide derivatives of soybean oil and fatty esters is reported. This entails first the formation of epoxides and then the azidization of the epoxides. The azidization reaction is carried out at high yields in water with only a small amount of an ionic liquid as a catalyst. The distribution of azide and alcohol functionalities on the fatty acid moiety is approximately random. This reaction has been applied to methyl oleate, methyl linoleate, soybean oil, and methyl soyate. The resulting structures have been studied by NMR.  相似文献   

Empirical modelling of tensile strength of woven fabrics     

Abhijit Majumdar  Anindya Ghosh  Shib Sankar Saha  Ayan Roy  Subir Barman  Dhrubajyoti Panigrahi  Anjan Biswas 《Fibers and Polymers》2008,9(2):240-245

Aesthetic properties of fabrics have been considered as the most important fabric attribute for years. However, recently there has been a paradigm shift in the domain of textile material applications and consequently more emphasis is now being given on the mechanical and functional properties of fabrics rather than its aesthetic appeal. Moreover, in certain woven fabrics used for technical applications, strength is a decisive quality parameter. In this work, tensile strength of plain woven fabrics has been predicted by using two empirical modelling methods namely artificial neural network (ANN) and linear regression. Warp yarn strength, warp yarn elongation, ends per inch (EPI), picks per inch (PPI) and weft count (Ne) were used as input parameters. Both the models were able to predict the fabric strength with reasonably good precision although ANN model demonstrated higher prediction accuracy and generalization ability than the regression model. The warp yarn strength and EPI were found to be the two most significant factors influencing fabric strength in warp direction.  相似文献   

Reproduction,growth and stress response in adult red sea bream,Pagrus major (Temminck & Schlegel) exposed to different photoperiods at spawning season     

Amal Biswas  Manabu Seoka  Hiroyuki Inagaki  Kenji Takii 《Aquaculture Research》2010,41(4):519-527

Adult red sea bream, Pagrus major (body weight, 1.0–2.0 kg) was exposed to three photoperiods [12 h light:12 h dark (12L:12D), 16 h light:8 h dark (16L:8D) and 24 h light:0 h dark (24L:0D)] from 2 months before spawning till the end of the spawning season to investigate growth, spawning and stress response. During the spawning season, tanks were checked every morning for spawned eggs. The growth performance in fish under 24L:0D was stimulated with significantly higher feed intake than those under other photoperiods (P<0.05). The number of eggs and gonadal histology confirmed that three and five females out of six in each of duplicate tanks of the 16L:8D treatment spawned. In contrast, only two out of six females in one tank of the 24L:0D treatment spawned, and no spawns were observed in the 12L:12D treatment. At the end of the spawning period, both 17β‐estradiol and testosterone levels were significantly higher in fish exposed to 16L:8D followed by 12L:12D and 24L:0D photoperiods (P<0.05). Photoperiod manipulation did not cause significant stress response in fish (P>0.05). The results suggest that stimulating the growth performance of red sea bream at reproductive stage with a 24L:0D photoperiod is possible if the fish are subjected to this photoperiod long before the onset of the spawning season.  相似文献   

Effect of rotifer enrichment with herbal extracts on growth and resistance of red sea bream,Pagrus major (Temminck & Schlegel) larvae against Vibrio anguillarum     

Osamu Takaoka  Seung‐Cheol Ji  Katsuya Ishimaru  Si‐Woo Lee  Gwan‐Sik Jeong  Junichi Ito  Amal Biswas  Kenji Takii 《Aquaculture Research》2011,42(12):1824-1829

The effects of the dietary medicinal herbs Massa medicata, Crataegi fructus (Cf), Artemisia capillaries, Cnidium officinale and their mixture (HM), on growth and resistance against Vibrio anguillarum were examined using larval red sea bream, Pagrus major. The methanol extracts of Cf and HM inhibited the proliferation of seven infectious bacterial strains including the genera Aeromonas, Edwasiella, Photobavterium, Pseudomonas and Vibrio. Rotifers enriched with emulsified bonito oil containing extracts of Cf and HM lowered the bacterial counts in their body on TCBS medium for the genus Vibrio in comparison with rotifers enriched by emulsified bonito oil alone. Although statistically not significant, larvae fed rotifer fortified with Cf from 3 to 20 days after hatching showed the best growth and final body weight followed by those given rotifer with HM and control enrichment. However, final body length of larvae fed rotifer with Cf was significantly larger than larvae fed on rotifers enriched with other herb extracts. After a challenge test with V. anguillarum, survival of larvae fed rotifer with Cf and HM was significantly higher than for the control treatment. These results revealed that Cf and HM in rotifer is useful to promote growth and resistance against V. anguillarum in red sea bream larvae, providing a new technology for mass production of disease‐resistant fry and fingerlings.  相似文献