Co-inoculation of potassium solubilizing and nitrogen fixing bacteria on solubilization of waste mica and their effect on growth promotion and nutrient acquisition by a forage crop     

Biraj Bandhu Basak  Dipak Ranjan Biswas 《Biology and Fertility of Soils》2010,46(6):641-648

Waste mica, a potassium-bearing mineral, is a by-product of mica industry; however, its potassium (K)-supplying capacity for crop production is not well understood. A greenhouse trial was made to study the effect of co-inoculation of potassium solubilizing (Bacillus mucilaginosus) and nitrogen (N) fixing (Azotobacter chroococcum A-41) bacteria on solubilization of waste mica (a potassium-bearing mineral) and their effects on growth promotion and nutrient uptake by a forage crop of sudan grass (Sorghum vulgare Pers.) in a Typic Haplustalf. Results revealed that significantly higher biomass accumulation and nutrient acquisition were obtained in all the pots treated with mica and/or bacterial strain as compared to control. Data indicated that co-inoculation of waste mica with B. mucilaginosus and A. chroococcum A-41 resulted in highest biomass production and nutrient acquisition. Co-inoculation of bacterial strains maintained consistently highest amounts of available K and N in soils even at 150 days of crop growth than other treatments. B. mucilaginosus strain was more effective and potent K solubilizer than A. chroococcum A-41. Thus, co-inoculation of potassium solubilizing and nitrogen fixing bacteria to waste mica could be a promising and alternative option for utilizing this potent source as K fertilizer to crops and maintaining greater nutrients availability in soil. Further studies are necessary to see the effects of these bacterial strains on mobilization of potassium-bearing minerals under field conditions.  相似文献   

Evidence of mixed infection of peste des petits ruminants virus and bluetongue virus in a flock of goats as confirmed by detection of antigen, antibody and nucleic acid of both the viruses     

B. Mondal  A. Sen  K. Chand  S. K. Biswas  A. De  K. K. Rajak  S. Chakravarti 《Tropical animal health and production》2009,41(8):1661-1667

A mixed infection with peste des petits ruminants virus (PPRV) and bluetongue virus (BTV) occurred in goats which exhibited symptoms characteristic of PPR. A number of samples were collected from ailing or dead goats for labrotory diagnosis. Antibody to BTV and PPRV was detected in sera samples by competitive ELISA. No PPRV antigen was detected in tissue samples like lung and spleen, however, presence of PPRV antigen in some sera samples was confirmed by sandwich ELISA. All the blood samples collected from the ailing animals were found positive for BTV antigen by a sandwich ELISA. BTV- and PPRV nucleic acids were amplified from the pooled blood and tissue samples respectively by RT-PCR assays. The identity of the amplicons was confirmed by cloning and sequencing. All these tests confirm that the goats were infected with PPRV and BTV simultaneously. Isolation of viruses from the clinical samples is underway.  相似文献   

Comparative analysis of genetic diversity in Citrus germplasm collection using AFLP,SSAP, SAMPL and SSR markers     

Manosh Kumar Biswas  Lijun Chai  Mohamed Hamdy Amar  Xianlong Zhang  Xiu-xin Deng 《Scientia Horticulturae》2011

In this study we evaluate the informativeness and efficiency of Amplified Fragment Length Polymorphism (AFLP), Sequence-Specific Amplified Polymorphism (S-SAP), Selectively Amplified Microsatellite Polymorphic Loci (SAMPL) and Simple Sequence Repeat (SSR) markers for genetic diversity, phylogenetic relationship among the Citrus species and mapping ability of the marker system. The SSR exhibited relatively higher level of polymorphism information content in terms of the expected heterozygosity, than that of the AFLPs, SSAPs and SAMPLs. For each marker system, average level of the discriminating potential was very close to the actual discriminating potential. Similarity matrices showed weak, yet significant correlations when Mantel's test was applied. The highest positive (0.72) correlation was found between the AFLP and SSAP markers. The SSR and SAMPL markers were poorly correlated. The dendrogram topology among the four marker systems had high similarity. Taken together, the SSAP and SAMPL were highly efficient in detecting genetic similarity in Citrus, while the SSR may be more useful for segregation studies and genome mapping in Citrus. The SSAP and SAMPL markers could be useful for Citrus genome mapping in combination with AFLP and SSR markers. To our knowledge, this was the first detail report of a comparison of performances among AFLP, SSR and retrotrasposon based molecular marker technique on a set of samples of Citrus. Our result provides guidance for future efficient use of these molecular methods in genetic analysis of Citrus sp. and its relatives.  相似文献   

Senescence of Flag Leaf and Glume in Rice: Role of Grains during Source-Sink Modification by Physical and Chemical Means   总被引：1，自引：0，他引：1  

A. K. Biswas  S. K. Mandal 《Journal of Agronomy and Crop Science》1993,171(1):13-19

Senescence of flag leaf and glume of rice ( Oryza sativa L. cv. Ratna ) at different source sink ratios, through manipulating the plants by physical and chemical means, were analyzed. As judged from the chlorophyll retention capacity, induction of sterility deferred senescence of flag leaf and glume at both grain filling and post grain maturation period. Senescence of the glumes was promoted in the plants whose panicles were darkened. The time needed for achieving maximum 1000 grain dry weight was quickened, the harvest index (HI) was lowered and the flag leaf senescence was delayed by such manipulation. Spraying of urea deferred senescence of the flag leaf as well as increased 1000 grain dry weight and HI. The time taken for achieving maximum 1000 grain dry weight was, however, delayed. Senescence of glumes was promoted by α-HPMS spray, while the same was delayed by PMA spray on the whole plant. Both the chemicals deferred flag leaf senescence and decreased 1000 grain dry weight and HI. Senescence of the flag leaf at post grain filling period was also delayed by the above physical and chemical manipulations compared to that of control, the highest delaying effect was recorded in the flag leaf of sterile plant.
It is concluded that nutrient drainage is the primary cause of nonsequential senescence of the flag leaf of rice (cv. Ratna ). The involvement of senescence signal if any, is perhaps of secondary importance.  相似文献   

Evaluation of microbial indicators for the determination of bacterial groundwater contamination sources     

M. Cimenti  N. Biswas  J. K. Bewtra  A. Hubberstey 《Water, air, and soil pollution》2005,168(1-4):157-169

Several different Microbial source tracking methods (MSTs) can be used to distinguish human from animal fecal contamination in water; In this study, experiments were carried out to test the effectiveness and reliability of three bacteria based approaches (the fecal coliforms to fecal streptococci ratio, antibiotic-resistant Clostridium perfringens, and human bifidobacteria) in a simulated groundwater micro-environment. The methods were evaluated in three phases: initially, the specificity of each indicator was validated on groundwater samples affected by known pollution source; then the variation of performance with time of each method was determined, and finally, the die-off coefficients for pure species of Clostridium perfringens and Bifidobacterium adolescentis were measured. The results indicate that only the determination of human bifidobacteria concentration can be considered reliable in distinguishing human from animal pollution in groundwater at the conditions applied. Nevertheless, human bifidobacteria were detectable only for two weeks after the contamination event. This study also shows that antibiotic resistant Clostridium perfringens detected using the Shahidi-Ferguson medium is not source specific, whereas it confirms that this species can be useful for timing general fecal contamination events.  相似文献   

Soil properties and crop yields on a vertisol in India with application of distillery effluent     

K.M. Hati  A.K. Biswas  K.K. Bandyopadhyay  A.K. Misra 《Soil & Tillage Research》2007,92(1-2):60-68

Distillery effluent, a foul smelling, dark coloured by-product of distillery industries, is usually applied as irrigation water or as an amendment to arable land in some areas which are in the vicinity of the distillery industries. A field experiment on soybean–wheat system was conducted for 3 consecutive years in a Vertisol of central India to evaluate the effect of distillery effluent (DE) as an amendment on soil properties and crop productivity. The treatments were control (no fertilizer or manure or DE, T₁), 100% NPK + FYM @ 4 Mg ha⁻¹ to soybean (T₂) and four graded levels of DE, viz.: 2.5 cm DE to soybean and wheat on residual nutrition (T₃), 2.5 cm DE to soybean and 1.25 cm to wheat (T₄), 5 cm DE to soybean and wheat on residual nutrition (T₅), 5 cm DE to soybean and 2.5 cm to wheat (T₆). The organic carbon, microbial biomass carbon and electrical conductivity (EC) of the surface (0–10 cm) soil increased significantly with application of DE compared to T₁ and T₂, but the soil pH was not affected. The EC increased from 0.47 dS m⁻¹ and 0.58 dS m⁻¹, respectively, in T₁ and T₂ to 1.52 dS m⁻¹ in T₆, where highest dose of DE was applied. This indicated a slight build-up of salinity with DE application. The application of DE showed a significant improvement in the physical properties of the soil. The mean weight diameter (MWD), saturated hydraulic conductivity, water retention at field capacity and available water content were significantly (P < 0.05) higher, while bulk density (BD) and penetration resistance of the surface soil were significantly lower (P < 0.05) in all DE treated plots except in T₃ than those in T₁ and T₂. The fractions of WSA of more than 1 mm diameter in T₆, T₅ and T₄ were, respectively, 141%, 107% and 116% more than the control. The MWD showed a positive linear relationship with the organic carbon (r = 0.84^**) and microbial biomass carbon (r = 0.90^**) of the soil. A significant (P < 0.01) negative linear relationship (r = 0.70^**) was found between soil organic carbon and BD. Except T₃, all the DE treated plots recorded significantly higher total and microporosity of the soil than control. Water retention at permanent wilting point and macroporosity of the soil were not affected by treatment. The seed yield of soybean in all the DE treatments was similar with T₂ (1.86 Mg ha⁻¹) but significantly more than control (1.28 Mg ha⁻¹). The DE application levels have not affected the seed yield of soybean. In wheat highest grain yield was recorded in T₂ (3.47 Mg ha⁻¹), which was similar with T₄ (3.16 Mg ha⁻¹), T₅ (3.22 Mg ha⁻¹) and T₆ (3.46 Mg ha⁻¹). DE application up to T₄ level was found suitable from productivity, salinity and sustainability point of view. The study showed that judicious application of DE as an amendment to the agricultural field could be considered as a viable option for safe disposal of this industrial waste.  相似文献   

Prediction of the movement of perchlor0ethylene in soil columns     

N. Biswas  Richard G. Zytner  J. A. McCorquodale  J. K. Bewtra 《Water, air, and soil pollution》1991,60(3-4):361-380

Many toxic volatile organic compounds (VOCs) are being released to the soil through improper waste disposal practices, industrial impoundments, landfills and accidental spills during transport and handling. Once released, the VOCs migrate toward the groundwater and cause contamination. One of the most frequently found VOCs in groundwater supplies is perchloroethylene (PCE). In this study an attempt was made to investigate the transport of PCE in two types of soil. PCE spills were created on soil columns in the laboratory and column breakthrough theory was used to analyze the data. Empirical equations were used to calibrate a contaminant transport model. The programs for this simple model have been prepared for use with IBM microcomputers and are capable to predicting the breakthrough time and concentration of PCE at various depths in the profile.  相似文献   

Field-level comparison of nitrogen rates and application methods on maize yield,grain quality and nitrogen use efficiency in a humid environment     

B. L. Ma  D. K. Biswas 《Journal of plant nutrition》2016,39(5):727-741

Appropriate nitrogen (N) management practices are of critical importance in improving N use efficiency (NUE), maize (Zea mays) yield and environmental quality. A six-year (2005–2010) on-farm trial was conducted in Ottawa, Canada to assess the effects of N rates and application methods on grain yield and NUE. In four out of the six-year study, grain yield increased by 60–77 kg ha^?1 by sidedress, compared to 49–66 kg ha^?1 for each kg N ha^?1 applied at preplant. Grain yield response to N between the two strategies was similar in the other growing seasons. Sidedress strategy required 15 kg N ha^?1 less of the maximum economic rate of N (MERN) than preplant application. Our results indicate that sidedress application of 90–120 kg N ha^?1 with a starter of 30 kg N ha^?1 resulted in greater yield, grain quality and NUE than preplant N application in this cool, humid and short growing-season region.  相似文献   

Effect of biochar on soil properties and infiltration in a light salinized soil: Experiments and simulations     

Yi Li  Chuncheng Liu  Zhijie Liang  Xiaofang Wang  Xiangyang Fan  De Li Liu  Asim Biswas 《European Journal of Soil Science》2022,73(4):e13279

  相似文献   

Radar detection of cloud-seeding effects   总被引：1，自引：0，他引：1  

Hobbs PV  Lyons JH  Locatelli JD  Biswas KR  Radke LF  Weiss RR  Rangno AL 《Science (New York, N.Y.)》1981,213(4513):1250-1252

The effects on precipitation of artificially seeding clouds with Dry Ice have been monitored from cloud to ground with a radar that has a wavelength of 8.6 millimeters.  相似文献