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Ultraperformance liquid chromatography coupled with hybrid quadrupole/ion mobility/orthogonal acceleration time-of-flight (oa-TOF) mass spectrometry (UPLC-IM-MS) was used to study the isomeric transformations of trans-5-caffeoylquinic acid, an extremely active compound present in multiple vegetables, fruits, and beverages. The UPLC/oa-TOF MS results proved that in phosphate buffer (pH 7.4), plasma, or urine sample, trans-5-caffeoylquinic acid first isomerizes to trans-4-caffeoylquinic acid and then to trans-3-caffeoylquinic acid by intramolecular acyl migration. When exposed to UV light, trans-3-, -4-, and -5-caffeoylquinic acids undergo cis/trans isomerization to form cis isomers. The isomerization was solely dependent on the pH of the matrix, as well as the incubation temperature, and was independent of metabolic enzymes. UPLC-IM-MS results revealed that a reversible cis/trans isomerization of caffeoylquinic acids could also be induced by the electric field in an electrospray source. Thus, understanding the possible role of electric field-induced isomerization of caffeoylquinic acids may help lessen the confusion between gas phase phenomena and liquid state chemistry when applying IM-MS analysis. The comprehensive understanding of caffeoylquinic acid isomerization transformations is crucial for the appropriate handling of samples and interpretation of experimental data.  相似文献   
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We studied in growing pigs the effects of exposure to dietary chlortetracycline on bone mineral density (BMD) and bone color. Pigs were randomly allocated to a drug-free diet (n = 48) or a diet fortified with 800 ppm of chlortetracycline, starting either at 28- or 84-d of age, and for either a 28- or 56-d duration (n = 16 pigs/group). The lumbar vertebral discoloration and BMD of randomly chosen pigs were evaluated at 28-d intervals up to 168-d of age. The odds of bone discoloration increased with dosing duration and age at treatment onset, and decreased with the withdrawal time and age at treatment onset interaction (p ? 0.001). The measured trabecular BMD linearly increased with age and squared treatment duration (p ? 0.005). Therefore, TC-induced bone discoloration is reversible, and may be prevented with proper dosing regimen design. Moreover, TC induces a persistent increase on BMD that could be detected with quantitative computed tomography.  相似文献   
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The single chromosome substitution lines of chromosome 3B of the Czech alternative wheat variety Česká Přesívka (CP 3B) into two spring varieties Zlatka and Sandra, revealed clear differences in flowering time compared to the recipient varieties. To map this gene(s), recombinant substitution lines for chromosome 3B were produced from crosses of the substitution lines with their recipient parents and genetic maps developed using SSR markers. Two populations were mapped, Sandra//Sandra 3B/Sandra (CP 3B) and Zlatka//Zlatka/Zlatka (CP 3B). Combining the genotype data with phenotype data on flowering time in five independent experiments under natural long day or controlled short day conditions revealed a single flowering time QTL. This gene had an additive effect of 1–6 days, depending on environment and genetic background, and was mapped in both populations to a position in the region of marker Xbarc164 near the centromere on the long arm of 3B. Comparisons of the genetic maps with other 3B maps developed by the authors indicated that the QTL may be homologous to a QTL segregating in UK germplasm.  相似文献   
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Anthropogenic radioisotopes with long physicalhalf-lives derived from atmospheric fallout remain inthe environment for decades after deposition. Process- and field-based studies within uplandcatchments show that radiocaesium is transported insolution as well as in particulate form. Catchmenthydrology is therefore an important control onradiocaesium transport and natural processes of soildevelopment. The topographic index, from thehydrological model TOPMODEL, has been used as a basisfor the development of a simple model for predictingradiocaesium redistribution in temperate uplandcatchments. The model is particularly suited topredicting 137Cs redistribution within uplandenvironments as it is based on topography, which isreadily calculated from digital terrain models withingeographical information systems. A conceptual modelof radiocaesium losses on hillslopes and re-depositionon the valley floor was calibrated with atmosphericweapons testing 137Cs inventories from soil coredata for the Raithburn catchment, Renfrewshire, U.K. The model fitted the observations well and showed thatin this catchment a topographic index value of about5.0, for 10 m grid cells, forms the threshold between137Cs loss and accumulation. The resultsindicated that about 20% of the total atmosphericweapons testing 137Cs deposited in the catchmenthad been transported out of the catchment over theca. 30 yr period since deposition.  相似文献   
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Objective – To present a simplified quantitative approach to acid‐base analysis and to demonstrate its clinical utility. Data Sources – Original research articles and textbooks. Data Synthesis – A simplified quantitative approach to acid‐base analysis is presented, which is derived from the Fencl‐Stewart approach and calculates the magnitude of the effect on the standardized base excess (SBE) of 5 separate variables: (1) a free water effect (marked by sodium concentration), (2) an effect marked by the chloride concentration, (3) an albumin effect, (4) a lactate effect, and (5) a phosphate effect. Six clinical cases with acid‐base abnormalities are presented in which the quantitative approach provides information that is not apparent from the traditional approach. Conclusion – This simplified quantitative approach provides a comprehensive evaluation of complex acid‐base disorders, identifies individual processes and their relative influence on SBE, and aids in the development of an appropriate therapeutic plan.  相似文献   
89.
A better understanding of imaging characteristics of equine stifle osteoarthritis (OA) may allow earlier detection and improve prognosis. Objectives of this ex vivo, prospective, methods comparison study were to (1) describe the location and severity of naturally acquired OA lesions in the equine stifle using ultrasound (US), radiography (XR), computed tomography (CT), and macroscopic evaluation (ME); (2) compare the diagnostic performance of each imaging modality with ME; and (3) describe subchondral bone mineral density (BMD) in equine stifle joints with OA using CT. Radiographic, CT, and US evaluations were performed on 23 equine cadaver stifles and compared with ME. Significant associations were found between osteophyte global scores for all imaging modalities (CT, P ? 0.0001; XR, P = 0.005; US, P = 0.04) vs. ME osteophyte global scores. Osteophytes were detected most frequently in the medial femorotibial (MFT) joint. A specific pattern of osteophytes was observed, with a long ridge of new bone at the insertion of the MFT joint capsule cranially on the medial femoral condyle. A novel caudo‐10°proximo‐5°lateral‐cranio‐disto‐medial oblique radiographic projection was helpful for detection of intercondylar osteophytes. Multiplanar CT reformatted images were helpful for characterizing all osteophytes. Osteophyte grades at most sites did not differ among modalities. Low sensitivity/specificity for subchondral bone sclerosis and flattening of femoral condyles suggested that these signs may not be reliable radiographic and CT indicators of equine stifle OA. Equine stifle OA was associated with a decrease in BMD and specific sites of focal subchondral bone resorption/cyst formation were found in some specimens.  相似文献   
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