全文获取类型
收费全文 | 950篇 |
免费 | 57篇 |
专业分类
林业 | 41篇 |
农学 | 19篇 |
基础科学 | 5篇 |
151篇 | |
综合类 | 124篇 |
农作物 | 72篇 |
水产渔业 | 68篇 |
畜牧兽医 | 446篇 |
园艺 | 26篇 |
植物保护 | 55篇 |
出版年
2023年 | 16篇 |
2022年 | 14篇 |
2021年 | 33篇 |
2020年 | 37篇 |
2019年 | 41篇 |
2018年 | 28篇 |
2017年 | 21篇 |
2016年 | 19篇 |
2015年 | 21篇 |
2014年 | 22篇 |
2013年 | 45篇 |
2012年 | 54篇 |
2011年 | 80篇 |
2010年 | 35篇 |
2009年 | 35篇 |
2008年 | 47篇 |
2007年 | 55篇 |
2006年 | 56篇 |
2005年 | 48篇 |
2004年 | 69篇 |
2003年 | 46篇 |
2002年 | 48篇 |
2001年 | 15篇 |
2000年 | 5篇 |
1999年 | 9篇 |
1998年 | 8篇 |
1997年 | 2篇 |
1996年 | 5篇 |
1995年 | 3篇 |
1994年 | 6篇 |
1993年 | 6篇 |
1992年 | 6篇 |
1991年 | 3篇 |
1990年 | 2篇 |
1989年 | 6篇 |
1988年 | 5篇 |
1987年 | 3篇 |
1984年 | 4篇 |
1983年 | 7篇 |
1980年 | 2篇 |
1979年 | 2篇 |
1978年 | 4篇 |
1977年 | 4篇 |
1976年 | 2篇 |
1975年 | 3篇 |
1973年 | 2篇 |
1972年 | 2篇 |
1971年 | 2篇 |
1966年 | 3篇 |
1936年 | 1篇 |
排序方式: 共有1007条查询结果,搜索用时 46 毫秒
61.
Memory B cells formed in response to microbial antigens provide immunity to later infections; however, the inability to detect rare endogenous antigen-specific cells limits current understanding of this process. Using an antigen-based technique to enrich these cells, we found that immunization with a model protein generated B memory cells that expressed isotype-switched immunoglobulins (swIg) or retained IgM. The more numerous IgM(+) cells were longer lived than the swIg(+) cells. However, swIg(+) memory cells dominated the secondary response because of the capacity to become activated in the presence of neutralizing serum immunoglobulin. Thus, we propose that memory relies on swIg(+) cells until they disappear and serum immunoglobulin falls to a low level, in which case memory resides with durable IgM(+) reserves. 相似文献
62.
Armstead I Donnison I Aubry S Harper J Hörtensteiner S James C Mani J Moffet M Ougham H Roberts L Thomas A Weeden N Thomas H King I 《Science (New York, N.Y.)》2007,315(5808):73
A key gene involved in plant senescence, mutations of which partially disable chlorophyll catabolism and confer stay-green leaf and cotyledon phenotypes, has been identified in Pisum sativum, Arabidopsis thaliana, and Festuca pratensis by using classical and molecular genetics and comparative genomics. A stay-green locus in F. pratensis is syntenically equivalent to a similar stay-green locus on rice chromosome 9. Functional testing in Arabidopsis of a homolog of the rice candidate gene revealed (i) senescence-associated gene expression and (ii) a stay-green phenotype after RNA interference silencing. Genetic mapping in pea demonstrated cosegregation with the yellow/green cotyledon polymorphism (I/i) first reported by Gregor Mendel in 1866. 相似文献
63.
Rosalind Bryce Matthew K. Oliver Llinos Davies Helen Gray Jamie Urquhart Xavier Lambin 《Biological conservation》2011,(1):575-583
Successful eradications of harmful invasive species have been mostly confined to islands while control programs in mainland areas remain small, uncoordinated and vulnerable to recolonisation. To allow the recovery of threatened native species, innovative management strategies are required to remove invasives from large areas. We took an adaptive approach to achieve large scale eradication of invasive American mink in North East Scotland. The project was centred on the Cairngorms National Park (Scotland), with the primary aim of protecting endangered water vole populations. The project was initiated by scientists and supported and implemented through a partnership comprising a government agency, national park authority and local fisheries boards. Capitalising on the convergent interests of a diverse range of local stakeholders, we created a coordinated coalition of trained volunteers to detect and trap mink. Starting in montane headwaters, we systematically moved down river catchments, deploying mink rafts, an effective detection and trapping platform. Volunteers took increasing responsibility for raft monitoring and mink trapping as the project progressed. Within 3 years, the project removed 376 mink from 10570 km2 with the involvement of 186 volunteers. Capture rate within sub-catchments increased with greater connectivity to mink in other sub-catchments and with proximity to the coast where there is more productive habitat. The main factor underpinning the success of this project was functional volunteer participation. The project is a reason for optimism that the tide of invasion can be rolled back on a large scale where the convergent interest of local communities can be harnessed. 相似文献
64.
Conservation problems typically involve groups with competing objectives and strategies. Taking effective conservation action requires identifying dependencies between competing strategies and determining which action optimally achieves the appropriate conservation goals given those dependencies. We show how several real-world conservation problems can be modeled game-theoretically. Three types of problems drive our analysis: multi-national conservation cooperation, management of common-pool resources, and games against nature. By revealing the underlying structure of these and other problems, game-theoretic models suggest potential solutions that are often invisible to the usual management protocol: decision followed by monitoring, feedback and revised decisions. The kind of adaptive management provided by the game-theoretic approach therefore complements existing adaptive management methodologies. 相似文献
65.
66.
SURGICAL FINDINGS: an adenocarcinoma of the uterine stump with abdominal metastases is described in a 12.5-year-old incompletely ovariohysterectomised domestic shorthair cat. At the time of presentation, the adenocarcinoma had metastasised to the right perirenal lymph node, the abdominal aorta and the right ureter, resulting in the formation of a large cystic structure. This had compressed and displaced surrounding structures, including the abdominal vena cava and the right kidney, and formed multiple adhesions to the body wall and adjacent abdominal structures. Metastatic extension to the aorta had resulted in its regression into a 2 mm diameter non-pulsatile vessel. PRACTICAL RELEVANCE: only one case of uterine adenocarcinoma has previously been reported in an ovariohysterectomised cat. As such, this represents a very unusual and severe complication following an incomplete ovariohysterectomy. Invasion of the tumour tissue into surrounding structures created further complications. 相似文献
67.
Maria Fernanda de M. Costa Adriana K. Carmona Marcio F. M. Alves Timothy M. Ryan Helen M. Davies Garry A. Anderson Ron F. Slocombe 《Journal of veterinary science (Suw?n-si, Korea)》2011,12(1):21-25
Angiotensin-I converting enzyme (ACE) is a key regulator of blood pressure, electrolytes and fluid homeostasis through conversion of angiotensin I into angiotensin II. Recently, a genetic polymorphism of the ACE gene, which accounts for 47% of the variation of ACE activity in blood, has been advocated as a biomarker of athletic aptitude. Different methods of analysis and determination of ACE activity in plasma have been used in human and equine research without a consensus of a "gold standard" method. Different methods have often been used interchangeably or cited as being comparable in the existing literature; however, the actual agreement between assays has not been investigated. Therefore, in this study, we evaluated the level of agreement between three different assays using equine plasma obtained from 29 horses. Two spectrophotometric assays using Furylacryloyl-phenylalanyl-glycyl-glycine as substrate and one fluorimetric assay utilizing o-aminobenzoic acid-FRK-(Dnp)P-OH were employed. The results revealed that the measurements from the different assays were not in agreement, indicating that the methods should not be used interchangeably for measurement of equine ACE activity. Rather, a single method of analysis should be adopted to achieve comparable results and critical appraisal of the literature is needed when attempting to compare results obtained from different assays. 相似文献
68.
Imogen C. Johns BVSc DACVIM Anne Desrochers DVM DACVIM Kathryn L. Wotman DVM DACVIM Raymond W. Sweeney VMD DACVIM 《Journal of Veterinary Emergency and Critical Care》2011,21(3):273-278
Objective – To describe the clinical presentation, case management, and outcome in 2 foals with Rhodococcus equi infection associated with presumptive severe immune‐mediated hemolytic anemia. Series Summary – Two foals diagnosed with R. equi pneumonia on the basis of tracheal wash cultures, thoracic radiographs, and thoracic ultrasonography were concurrently diagnosed with hemolytic anemia. Both foals required whole blood transfusions, and were treated with the antimicrobial combination of rifampin and a macrolide (eg, clarithromycin, erythromycin, or azithromycin). Dexamethasone was used to prevent further hemolysis in both foals, and to treat acute lung injury/acute respiratory distress syndrome in 1 of the foals. Both foals survived, and required prolonged antimicrobial therapy. New or Unique Information Provided – Although extra‐pulmonary disorders are commonly diagnosed in foals infected with R. equi, hemolytic anemia is rarely described. Dexamethasone is considered the treatment of choice for immune‐mediated hemolytic anemia, but may be contra‐indicated in foals with severe bacterial infections. In these foals, a relatively low dose and short duration of dexamethasone was utilized in an attempt to minimize immune suppression, although early discontinuation in 1 foal precipitated a second hemolytic crisis. 相似文献
69.
Lynn H. Booth Vanessa J. Heppelthwaite Kathryn O’Halloran 《Journal of Soils and Sediments》2002,2(3):148-154
Objectives and Methods. The sensitivity of two biomarkers, the neutral red retention assay (NRRA) and cytochrome P450 were evaluated in the earthwormAporrectodea caliginosa for their potential to detect exposure to benzo[a]pyrene (BaP). Cytochrome P450 was also evaluated in the earthwormLumbricus rubellus, and measured using the substrate ethoxycoumarin. Optimal assay conditions (pH, and temperature) were determined, followed
by exposure of earthworms to 20 mg/kg BaP (a typical concentration at contaminated sites in New Zealand). Ethoxycoumarin-O-dealkylase
(ECOD) activity was measured after 7, 14, and 21 days exposure. The NRRA was evaluated in earthworms exposed to 0.2, 20, and
100 mg BaP/kg, and biomarker responses were compared with effects on body weight.
Results and Discussion Benzo[a]pyrene failed to induce ECOD activity in either earthworm species, and therefore it is not useful as a biomarker
of BaP exposure and was not evaluated further. In all cases, the NRRA was significantly affected in the absence of any effects
on earthworm body weight, indicating that this assay can detect exposure to BaP at a range of concentrations comparable to
those found at contaminated sites. The NRRA should be linked to reproductive endpoints, then it can be used as an early warning
indicator of adverse effects. Establishing biomarker stability under environmental conditions is an important step in biomarker
development. Therefore, the effects of soil type (sandy soil, silt loam, and a clay soil), moisture content (15–30%), and
temperature (5–20°) on the NRRA were determined. In all cases, there was no effect on the NRRA, indicating that this assay
is very stable under varying environmental conditions.
Conclusion and Outlook In conclusion, cytochrome P450 activity does not appear to be a useful indicator of PAH exposure in eitherA. caliginosa or L.rubellus, and due to the inherently low activity, it is not suitable as a routine biomarker for detecting environmental contamination
by these compounds. In contrast, the NRRA in the earthworm A.caliginosa is a promising indicator of PAH exposure at the concentrations likely to be found in contaminated sites in New Zealand, and
therefore has potential for evaluating these contaminated sites. If the NRRA can be linked to ecologically important life-cycle
endpoints, such as reproduction, then it could be used as an early warning indicator of adverse effects at contaminated sites,
i.e., by measuring biomarker responses in earthworms from a ‘contaminated area’ and comparing these with earthworms from a
matched control area. 相似文献
70.
David J. Anderson Kathryn P. Huyvaert Dana R. Wood Cynthia L. Gillikin Barrie J. Frost Henrik Mouritsen 《Biological conservation》2003,110(3):165-373
Albatross populations worldwide are threatened by incidental takes in longline fishery operations. The recent establishment of the Galápagos Marine Reserve (GMR) is relevant to the longline bycatch issue, as it prohibits industrial longlining in the vicinity of the major nesting site of waved albatrosses (Phoebastria irrorata). However, the legality of the fishing protections is being challenged, highlighting a need for data on use of the GMR by albatrosses. We used satellite tracking over a total of four breeding seasons to determine the distribution of waved albatrosses inside and outside the GMR, and thereby assess the degree of protection that GMR provisions offer to this species. During the incubation period, breeding adults made commuting trips from the nesting island (Isla Española) to the Peruvian upwelling zone, traveling north, east, and south after leaving the nest. During the brooding period, the distribution contracted markedly, and most satellite fixes were within the GMR. During the rearing period, breeders performed both long trips outside the GMR and short trips within. The southeastern portion of the GMR is used throughout the incubation, brooding, and early rearing periods by breeding waved albatrosses. Indirect information from non-breeding adults indicates that they are likely to use the waters of the GMR extensively. 相似文献