Single spaghetti tagging as a high-retention marking method for Japanese common sea cucumber <Emphasis Type="Italic">Apostichopus japonicus</Emphasis>     

Takumi Fujino  Hideki Sawada  Hiromichi Mitamura  Reiji Masuda  Nobuaki Arai  Yoh Yamashita 《Fisheries Science》2017,83(3):367-372

Populations of sea cucumbers, including the Japanese common sea cucumber Apostichopus japonicus, have been seriously depleted worldwide due to overfishing. Mark–recapture study is an efficient means of collecting ecological data. However, the use of such a method in sea cucumbers is difficult because they lack hard tissues in the body wall. Here we tested the viability of various tagging methods on A. japonicus. First, we applied conventional tags using four different methods [single spaghetti (T-bar) tagging, double spaghetti tagging, ribbon tagging, and Atkins tagging] to ten individuals per method in aquaria for 14 days. Of the methods used, single spaghetti tagging had the highest retention rate. Then we examined the retention rate of single spaghetti tags on ten individual sea cucumbers for up to approximately 6 months in rearing conditions. The single spaghetti tagging method showed a retention rate of 100% over at least 7 days, and 50% of the tags remained embedded after 56 days. The longest duration of tag retention was 174 days, at which time the experiment was terminated. These results indicate that single spaghetti tagging is reliable for both short- and longer-term studies, making it a useful tool for ecological and conservation studies in sea cucumbers.  相似文献   

Spontaneous polyploidization in critically endangered <Emphasis Type="Italic">Acipenser mikadoi</Emphasis>     

Miloš Havelka  He Zhou  Seishi Hagihara  Masaki Ichimura  Takafumi Fujimoto  Etsuro Yamaha  Shinji Adachi  Katsutoshi Arai 《Fisheries Science》2017,83(4):587-595

We investigated the source of spontaneous polyploidization in the critically endangered Acipenser mikadoi. Fourteen sib progeny of A. mikadoi and 11 hybrids between an A. mikadoi female and a Huso dauricus male, all showing atypically high ploidy, were analysed. Parent assignment based on five highly polymorphic microsatellite markers confirmed spontaneous duplication of maternal chromosome sets via retention of the second polar body to be the source of spontaneous polyploidization. To our knowledge, this provides the first evidence of the maternal origin of spontaneous polyploidization in A. mikadoi. Factorial correspondence analysis of the multilocus microsatellite genotypes placed the parent fish of spontaneous polyploids in clearly delineated clusters of A. mikadoi and H. dauricus, and parent fish had mitochondrial control region haplotypes corresponding to their presumed species. Thus, parent fish were confirmed to be of pure genetic origin, and hybridization did not promote the observed spontaneous polyploidization.  相似文献   

Collection,preservation and distribution of Oryza genetic resources by the National Bioresource Project RICE (NBRP-RICE)     

Yutaka Sato  Katsutoshi Tsuda  Yoshiyuki Yamagata  Hiroaki Matsusaka  Hiromi Kajiya-Kanegae  Yuri Yoshida  Ayumi Agata  Kim Nhung Ta  Sae Shimizu-Sato  Toshiya Suzuki  Misuzu Nosaka-Takahashi  Takahiko Kubo  Shoko Kawamoto  Ken-Ichi Nonomura  Hideshi Yasui  Toshihiro Kumamaru 《Breeding Science》2021,71(3):291

Biological resources are the basic infrastructure of bioscience research. Rice (Oryza sativa L.) is a good experimental model for research in cereal crops and monocots and includes important genetic materials used in breeding. The availability of genetic materials, including mutants, is important for rice research. In addition, Oryza species are attractive to researchers for both finding useful genes for breeding and for understanding the mechanism of genome evolution that enables wild plants to adapt to their own habitats. NBRP-RICE contributes to rice research by promoting the usage of genetic materials, especially wild Oryza accessions and mutant lines. Our activity includes collection, preservation and distribution of those materials and the provision of basic information on them, such as morphological and physiological traits and genomic information. In this review paper, we introduce the activities of NBRP-RICE and our database, Oryzabase, which facilitates the access to NBRP-RICE resources and their genomic sequences as well as the current situation of wild Oryza genome sequencing efforts by NBRP-RICE and other institutes.  相似文献   

日本柑橘黄龙病病原体电镜观察及PCR检测   总被引：3，自引：3，他引：0  

王运生  戴良英  荒井啓 《湖南农业大学学报(自然科学版)》2004,30(5):446-449

对日本柑橘黄龙病病原体的形态结构进行了电镜观察,结果发现柑橘黄龙病病菌日本株系绝大多数是球形和短椭圆形,极少观察到棒状形态,直径为100～500nm,外被一层胶状物质包围,厚约10～50nm,这与其他地区发现的柑橘黄龙病病原体形态不同．PCR检测表明,以硅藻土法提取的DNA为模板,利用Ready-To-Go^TM PCR bead法可以得到理想结果。  相似文献   

Effect of shelter acclimation on the post-release survival of hatchery-reared black-spot tuskfish <Emphasis Type="Italic">Choerodon schoenleinii</Emphasis>: laboratory experiments using the reef-resident predator white-streaked grouper <Emphasis Type="Italic">Epinephelus ongus</Emphasis>     

Yuuki Kawabata  Kimio Asami  Masato Kobayashi  Taku Sato  Koichi Okuzawa  Hideaki Yamada  Kenzo Yoseda  Nobuaki Arai 《Fisheries Science》2011,77(1):79-85

A critical component of many releasing projects is the identification and subsequent implementation of optimal release strategies that can decrease post-release predation mortalities. We performed laboratory experiments to investigate whether acclimation to shelters affects the post-release survival of hatchery-reared black-spot tuskfish Choerodon schoenleinii in the presence of a reef-resident predator, the white-streaked grouper Epinephelus ongus. Tuskfish were exposed to groupers under three different experimental conditions/treatments: (1) acclimation of fish to shelters prior to their exposure to groupers; (2) no acclimation of fish to shelters, but with shelters available during their exposure to groupers; (3) fish not acclimated to shelters and no shelters available during their exposure to groupers. Tuskfish that were acclimated to shelters utilized shelters more frequently than did non-acclimated fish, and the survival rate of acclimated fish was higher than those of fish in the other treatments. These results suggest that pre-release acclimation to shelters improves the post-release survival of hatchery-reared black-spot tuskfish.  相似文献