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101.
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103.
Arash Minai-Tehrani Young-Chan Park Soon-Kyung Hwang Jung-Taek Kwon Seung-Hee Chang Sung-Jin Park Kyeong-Nam Yu Ji-Eun Kim Ji-Young Shin Ji-Hye Kim Bitna Kang Seong-Ho Hong Myung-Haing Cho 《Journal of veterinary science (Suw?n-si, Korea)》2011,12(4):309-317
Conventional lung cancer therapies are associated with poor survival rates; therefore, new approaches such as gene therapy are required for treating cancer. Gene therapies for treating lung cancer patients can involve several approaches. Among these, aerosol gene delivery is a potentially more effective approach. In this study, Akt1 kinase-deficient (KD) and wild-type (WT) Akt1 were delivered to the lungs of CMV-LucR-cMyc-IRES-LucF dual reporter mice through a nose only inhalation system using glucosylated polyethylenimine and naphthalene was administrated to the mice via intraperitoneal injection. Aerosol delivery of Akt1 WT and naphthalene treatment increased protein levels of downstream substrates of Akt signaling pathway while aerosol delivery of Akt1 KD did not. Our results showed that naphthalene affected extracellular signal-regulated kinase (ERK) protein levels, ERK-related signaling, and induced Clara cell injury. However, Clara cell injury induced by naphthalene was considerably attenuated in mice exposed to Akt1 KD. Furthermore, a dual luciferase activity assay showed that aerosol delivery of Akt1 WT and naphthalene treatment enhanced cap-dependent protein translation, while reduced cap-dependent protein translation was observed after delivering Akt1 KD. These studies demonstrated that our aerosol delivery is compatible for in vivo gene delivery. 相似文献
104.
Dong-Kun Yang Young-Nam Park Gyeong-Soo Hong Hee-Kyung Kang Yoon-I Oh Soo-Dong Cho Jae-Young Song 《Journal of veterinary science (Suw?n-si, Korea)》2011,12(1):57-63
The nucleoprotein (N) and glycoprotein (G) of 11 Korean rabies virus (RABV) isolates collected from animals diagnosed with rabies between 2008 and 2009 were subjected to molecular and phylogenetic analyses. Six isolates originated from domestic animals (cattle and dogs) and five were obtained from wild free-ranging raccoon dogs. The similarities in the nucleotide sequences of the N gene among all Korean isolates ranged from 98.1 to 99.8%, while those of the G gene ranged from 97.9 to 99.3%. Based on the nucleotide analysis of the N and G genes, the Korean RABV isolates were confirmed as genotype I of Lyssavirus and classified into four distinct subgroups with high similarity. Phylogenetic analysis showed that the Korean isolates were most closely related to the non-Korean NeiMeng1025B and 857r strains, which were isolated from rabid raccoon dogs in Eastern China and Russia, respectively. These findings suggest that the Korean RABV isolates originated from a rabid raccoon dog in Northeastern Asia. Genetic analysis of the Korean RABV isolates revealed no substitutions at several antigenic sites, indicating that the isolates circulating in Korea may be pathogenic in several hosts. 相似文献
105.
106.
K.‐C. Cho Y.‐J. Han S.‐J. Kim S.‐S. Lee O.‐J. Hwang P.‐S. Song Y.‐S. Kim J.‐I. Kim 《Plant pathology》2011,60(4):631-639
A pepper esterase (PepEST) gene was introduced into creeping bentgrass (Agrostis stolonifera) by Agrobacterium‐mediated transformation. Purified recombinant PepEST proteins were sufficient to inhibit the growth of the fungal pathogens Rhizoctonia solani AG2‐2 (IIIB) (causing brown patch) and Sclerotinia homoeocarpa (dollar spot), but not the oomycete responsible for pythium blight, Pythium aphanidermatum. PepEST proteins were most effective against R. solani. After genetic transformation of creeping bentgrass with PepEST, the genomic integration of transgenes bar and PepEST was confirmed by Southern blot analysis, and their expression was also validated by northern blot and western blot analyses. Disease severity on R. solani‐inoculated leaves of transgenic plants was <10% compared to ca. 50% in non‐transgenic plants. Microscopic observation of infected leaves indicated that PepEST inhibited the growth of hyphae upon fungal infection. 相似文献
107.
Lyons MM Yu C Toma RB Cho SY Reiboldt W Lee J van Breemen RB 《Journal of agricultural and food chemistry》2003,51(20):5867-5870
Resveratrol in the fruits of bilberry (Vaccinium myrtillus L.), the lowbush "wild" blueberry (Vaccinium angustifolium Aiton), the rabbiteye blueberry (Vaccinium ashei Reade), and the highbush blueberry (Vaccinium corymbosum L.) were measured using a new assay based on high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS). The LC-MS/MS assay provided lower limits of detection than previous methods for resveratrol measurement, 90 fmol of trans-resveratrol injected on-column, and a linear standard curve spanning >3 orders of magnitude. The recoveries of resveratrol from blueberries spiked with 1.8, 3.6, or 36 ng/g were 91.5 +/- 4.5, 95.6 +/- 6.5, and 88.0 +/- 3.6%, respectively. trans-Resveratrol but not cis-resveratrol was detected in both blueberry and bilberry samples. The highest levels of trans-resvertatrol in these specimens were 140.0 +/- 29.9 pmol/g in highbush blueberries from Michigan and 71.0 +/- 15.0 pmol/g in bilberries from Poland. However, considerable regional variation was observed; highbush blueberries from British Columbia contained no detectable resveratrol. Because blueberries and bilberries are often consumed after cooking, the effect of baking on resveratrol content was investigated. After 18 min of heating at 190 degrees C, between 17 and 46% of the resveratrol had degraded in the various Vaccinium species. Therefore, the resveratrol content of baked or heat-processed blueberries or bilberries should be expected to be lower than in the raw fruit. Although blueberries and bilberries were found to contain resveratrol, the level of this chemoprotective compound in these fruits was <10% that reported for grapes. Furthermore, cooking or heat processing of these berries will contribute to the degradation of resveratrol. 相似文献
108.
Therapeutic effect of hyaluronic acid on experimental osteoarthrosis of ovine temporomandibular joint. 总被引:3,自引:0,他引:3
C H Kim B J Lee J Yoon K M Seo J H Park J W Lee E S Cho J J Hong Y S Lee J H Park 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(10):1083-1089
A symptomatic relief by hyaluronic acid (HA, MW: 3.5 x 10(6)), which is synthesized by Streptococcus spp, was investigated in experimental ovine osteoarthrosis. Bilateral osteoarthrosis (OA) of the temporo-mandibular joints (TMJs) was induced by perforating discs and by scrapping subchondral condylar surface. HA was intra-articularly injected into the left joints of 6 sheep on 7, 10, 14, 17 and 21 days after the operation and physiological saline as the control was injected into the contralateral (right) joints on the same day. Three sheep were killed at I month post-operation (MPO) and the remaining three sheep were killed at 3 MPO. Various responses such as proliferation of fibrous tissue, denudation, erosion, osteophyte formation, subcortical cyst formation and ankylosis were observed radiographically and histopathologically. The treatment of HA ameliorated the degenerative changes and lowered the osteoarthrotic score in the left joints at I MPO (9.96 vs 5.81) and 3 MPO (10.86 vs 5.29) compared to the right joints. These results indicate that a repeated intra-articular injection of HA inhibits the progression of OA in ovine TMJs by inducing the development of articular cartilage and by reducing the proliferation of fibrotic tissue. 相似文献
109.
A survey of the aerobic bacteria in the feces of captive raptors 总被引:5,自引:0,他引:5
Feces of 47 captive raptors belonging to the order Falconiformes or Strigiformes were cultured for bacteria. Gram-negative bacteria, which were cultured from the feces of 45 of the 47 raptors, were the most common isolates. A wide variety of species were identified, including a newly described genus (Moellerella wisconsensis), two newly described species (Escherichia fergusonii and Proteus penneri), and a member of a newly described enteric group (CDC Enteric group 41). Additional organisms identified that have not been reported in previous bacteriological surveys of raptors were Salmonella heidelberg, Salmonella braenderup, Morganella morganii, Yersinia ruckeri, Serratia spp., and Kluyvera sp. Escherichia coli, isolated from the feces of 42 of the 47 raptors, was the most frequently recovered. Streptococcus faecalis, the second most common isolate, was cultured from 30 birds. Several differences were observed between fecal bacteria isolated from raptors fed commercially prepared chicken and those isolated from raptors not fed chicken. The most obvious difference was that birds fed chicken had more varied gram-negative bacterial species and in greater numbers per fecal sample. The potential for the isolated bacteria from raptors as pathogens in humans and avian species is discussed. 相似文献
110.
D Y Cho R A Frey M M Guffy H W Leipold 《American journal of veterinary research》1975,36(11):1597-1603
Toxic effects of excessive vitamin A and of excessive vitamin ADE were studied in 9 mixed Labrador Retriever pups. Clinical signs were loss of body weight, dullness, emaciation, roughened coat, evident pain in limb joints, and retarded growth. Radiologic changes were decreases in overall length and thickness of long bones, development of osteophytes, periosteal reaction, and premature closure of epiphyses. Pathologic changes were degenerative epiphyseal plate, hemorrhage and exostotic proliferation of periosteum, fatty liver, and microcalculi in kidney. Toxic effects of excessive vitamin A did not appear to be so great when it was administered as vitamin ADE. 相似文献