Subcutaneous Soft Tissue Sarcoma with Rhabdoid Features in a Dog     

Ayako Sayama  Keiko Okado  Masako Imaoka  Yusuke Yokouchi  Toshimasa Jindo  Wataru Takasaki 《Journal of toxicologic pathology》2014,27(2):131-138

A nine-year-old male beagle dog had a white spherical mass in the subcutis of the left lumbar region. Microscopically, spindle to oval cells diffusely proliferated in the fibrous and myxoid stroma. Many neoplastic cells showed rhabdoid features or vacuolated cytoplasm. Immunohistochemically, the neoplastic cells were positive for vimentin and S100 and partly positive for neuron-specific enolase and glial fibrillary acidic protein but were negative for von Willebrand factor, desmin and α-smooth muscle actin. Ultrastructurally, the neoplastic cells had abundant cytoplasmic processes and desmosome-like structures. Cytoplasmic inclusions of rhabdoid-featured cells in HE sections were composed of aggregates of intermediate filaments, and cytoplasmic vacuoles were identified as an invagination of cytoplasm. Although malignant peripheral nerve sheath tumor was suggested according to these results, the present case was diagnosed as a soft tissue sarcoma with rhabdoid features due to a lack of identification of the basal lamina under electron microscopy.  相似文献   

Flow cytometric enumeration of reticulocyte in the peripheral blood from canine infected with Babesia gibsoni     

T Uemura  S Suzuki  T Ohnishi 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1990,37(6):468-472

Peripheral blood samples from dogs infected with Babesia gibsoni were analyzed by a flow cytometer for the percentage of reticulocytes after staining with a membrane-permeable fluorochrome, thiazole orange. Though thiazole orange has been reported to stain human reticulocytes and Plasmodium-infected erythrocytes, number of positive cells determined by the flow cytometry did not include that of erythrocytes infected with Babesia gibsoni. Analysis of 51 samples revealed a correlation coefficient of 0.96 as compared to the conventional determination by light microscopy. Separation of reticulocytes from Babesia gibsoni-infected erythrocytes by flow cytometry with or without the stain remained unresolved.  相似文献   

Studies on absorption and hydrolysis of ethyl alpha-D-glucoside in rat intestine     

Mishima T  Tanaka K  Tsuge H  Sugita J  Nakahara M  Hayakawa T 《Journal of agricultural and food chemistry》2005,53(18):7257-7261

Ethyl alpha-D-glucoside (alpha-EG) is normally contained in Sake, which has been taken by Japanese people since ancient times. In this study, the intestinal absorption of alpha-EG was investigated using rat everted intestinal sac. Furthermore, the alpha-EG hydrolytic activity in rat intestine was compared with disaccharides hydrolytic activities, and the effects of alpha-EG on disaccharides hydrolysis were examined using crude enzyme preparation from rat intestinal acetone powder. Glucose liberated from alpha-EG was detected in a serosal solution of everted rat intestinal sac, but it was only less than 4% of absorbed intact alpha-EG. alpha-EG absorption into small intestinal tissue was reduced by elimination of sodium ion from the mucosal solution or under the presence of phlorizin. The hydrolytic activity for alpha-EG was detected in crude enzyme preparation from rat intestinal acetone powder, but it showed a low value as compared to those for disaccharides. alpha-EG showed mixed type inhibition for maltose and sucrose hydrolysis, but inhibitory concentrations of alpha-EG required for 50% inhibition for the maltose and sucrose hydrolysis were higher than those of arabinose and acarbose. In conclusion, a small amount of alpha-EG was hydrolyzed and most of it was absorbed via SGLT1 as an intact form in the rat small intestine, and the inhibitory effect of alpha-EG on disaccharides hydrolysis was weak.  相似文献   

Metabolism of fungicide diethofencarb in grape (Vitis vinifera L.): definitive identification of thiolactic acid conjugated metabolites     

Fujisawa T  Ichise-Shibuya K  Katagi T  Ruzo LO  Takimoto Y 《Journal of agricultural and food chemistry》2003,51(18):5329-5336

The metabolic fate of diethofencarb (isopropyl 3,4-diethoxycarbanilate) separately labeled with (14)C at the phenyl ring and 2-position of the isopropyl moiety was studied in grape (Vitis vinifera L.). The acetonitrile solution of (14)C-diethofencarb at a rate of 500 g a.i. ha(-)(1) was once applied topically to fruits or leaves at the maturity stage of fruits (PHI 35 days), and the plants were grown in the greenhouse until harvest. In the grape plants, diethofencarb was scarcely translocated to the untreated portion and was degraded more in the fruit as compared to the leaf. For the fruit, diethofencarb primary underwent O-deethylation at the 4-position of the phenyl ring to form the phenolic derivative, isopropyl 3-ethoxy-4-hydroxycarbanilate (0.9% of the total radioactive residue, TRR). This metabolite was successively transformed via conjugation with glucose at the phenolic hydroxy group (8.1-18.1% TRR) or with thiolactic acid at the 5-position of the phenyl ring (1.5-1.7% TRR). The thiolactic acid conjugate was further metabolized mainly to two different types of glucose conjugates at the 4-position of the phenyl ring (8.7-13.5% TRR) and the hydroxy group in the thiolactic acid moiety (6.4-7.3% TRR), as evidenced by (1)H NMR and atmospheric pressure chemical ionization-liquid chromatography-mass spectrometry together with cochromatographies with synthetic standards.  相似文献   

Validation of a food-frequency questionnaire for cohort studies in rural Japan     

Ogawa K  Tsubono Y  Nishino Y  Watanabe Y  Ohkubo T  Watanabe T  Nakatsuka H  Takahashi N  Kawamura M  Tsuji I  Hisamichi S 《Public health nutrition》2003,6(2):147-157

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The Physcomitrella genome reveals evolutionary insights into the conquest of land by plants     

Rensing SA  Lang D  Zimmer AD  Terry A  Salamov A  Shapiro H  Nishiyama T  Perroud PF  Lindquist EA  Kamisugi Y  Tanahashi T  Sakakibara K  Fujita T  Oishi K  Shin-I T  Kuroki Y  Toyoda A  Suzuki Y  Hashimoto S  Yamaguchi K  Sugano S  Kohara Y  Fujiyama A  Anterola A  Aoki S  Ashton N  Barbazuk WB  Barker E  Bennetzen JL  Blankenship R  Cho SH  Dutcher SK  Estelle M  Fawcett JA  Gundlach H  Hanada K  Heyl A  Hicks KA  Hughes J  Lohr M  Mayer K  Melkozernov A  Murata T  Nelson DR  Pils B  Prigge M  Reiss B  Renner T  Rombauts S  Rushton PJ 《Science (New York, N.Y.)》2008,319(5859):64-69

We report the draft genome sequence of the model moss Physcomitrella patens and compare its features with those of flowering plants, from which it is separated by more than 400 million years, and unicellular aquatic algae. This comparison reveals genomic changes concomitant with the evolutionary movement to land, including a general increase in gene family complexity; loss of genes associated with aquatic environments (e.g., flagellar arms); acquisition of genes for tolerating terrestrial stresses (e.g., variation in temperature and water availability); and the development of the auxin and abscisic acid signaling pathways for coordinating multicellular growth and dehydration response. The Physcomitrella genome provides a resource for phylogenetic inferences about gene function and for experimental analysis of plant processes through this plant's unique facility for reverse genetics.  相似文献   

Discovery of a Jupiter/Saturn analog with gravitational microlensing     

Gaudi BS  Bennett DP  Udalski A  Gould A  Christie GW  Maoz D  Dong S  McCormick J  Szymanski MK  Tristram PJ  Nikolaev S  Paczynski B  Kubiak M  Pietrzynski G  Soszynski I  Szewczyk O  Ulaczyk K  Wyrzykowski L;OGLE Collaboration  Depoy DL  Han C  Kaspi S  Lee CU  Mallia F  Natusch T  Pogge RW  Park BG;MuFUN Collaboration  Abe F  Bond IA  Botzler CS  Fukui A  Hearnshaw JB  Itow Y  Kamiya K  Korpela AV  Kilmartin PM  Lin W  Masuda K  Matsubara Y  Motomura M  Muraki Y  Nakamura S  Okumura T  Ohnishi K  Rattenbury NJ  Sako T  Saito T  Sato S 《Science (New York, N.Y.)》2008,319(5865):927-930

Searches for extrasolar planets have uncovered an astonishing diversity of planetary systems, yet the frequency of solar system analogs remains unknown. The gravitational microlensing planet search method is potentially sensitive to multiple-planet systems containing analogs of all the solar system planets except Mercury. We report the detection of a multiple-planet system with microlensing. We identify two planets with masses of approximately 0.71 and approximately 0.27 times the mass of Jupiter and orbital separations of approximately 2.3 and approximately 4.6 astronomical units orbiting a primary star of mass approximately 0.50 solar mass at a distance of approximately 1.5 kiloparsecs. This system resembles a scaled version of our solar system in that the mass ratio, separation ratio, and equilibrium temperatures of the planets are similar to those of Jupiter and Saturn. These planets could not have been detected with other techniques; their discovery from only six confirmed microlensing planet detections suggests that solar system analogs may be common.  相似文献   

Mutations in U4atac snRNA, a component of the minor spliceosome, in the developmental disorder MOPD I     

He H  Liyanarachchi S  Akagi K  Nagy R  Li J  Dietrich RC  Li W  Sebastian N  Wen B  Xin B  Singh J  Yan P  Alder H  Haan E  Wieczorek D  Albrecht B  Puffenberger E  Wang H  Westman JA  Padgett RA  Symer DE  de la Chapelle A 《Science (New York, N.Y.)》2011,332(6026):238-240

Small nuclear RNAs (snRNAs) are essential factors in messenger RNA splicing. By means of homozygosity mapping and deep sequencing, we show that a gene encoding U4atac snRNA, a component of the minor U12-dependent spliceosome, is mutated in individuals with microcephalic osteodysplastic primordial dwarfism type I (MOPD I), a severe developmental disorder characterized by extreme intrauterine growth retardation and multiple organ abnormalities. Functional assays showed that mutations (30G>A, 51G>A, 55G>A, and 111G>A) associated with MOPD I cause defective U12-dependent splicing. Endogenous U12-dependent but not U2-dependent introns were found to be poorly spliced in MOPD I patient fibroblast cells. The introduction of wild-type U4atac snRNA into MOPD I cells enhanced U12-dependent splicing. These results illustrate the critical role of minor intron splicing in human development.  相似文献   

Seven samurai to protect “our” food: the reform of the food safety regulatory system in Japan after the BSE crisis of 2001     

Keiko Tanaka 《Agriculture and Human Values》2008,25(4):567-580

Using the case of food safety governance reform in Japan between 2001 and 2003, this paper examines the relationship between science and trust. The paper explains how the discovery of the first BSE positive cow and consequent food safety scandals in 2001 politicized the role of science in protecting the safety of the food supply. The analysis of the Parliamentary debate focuses on the contestation among legislators and other participants over three dimensions of risk science, including “knowledge,” “objects,” and “beneficiaries.” The metaphor of “seven samurai” and the relationally situated roles of “samurai,” “bandits,” and “beneficiaries” are used to show that in the process of policy making certain moral and ethical expectations on a new expert institution for food safety were contested and negotiated to frame responsibilities and commitments of social actors for creating the food system based on trust.

Keiko TanakaEmail:

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Multigenic system controlling viral systemic infection determined by the interactions between Cucumber mosaic virus genes and quantitative trait loci of soybean cultivars     

Ohnishi S  Echizenya I  Yoshimoto E  Boumin K  Inukai T  Masuta C 《Phytopathology》2011,101(5):575-582

Soybean 'Harosoy' is resistant to Cucumber mosaic virus soybean strain C (CMV-SC) and susceptible to CMV-S strain D (CMV-SD). Using enzyme-linked immunosorbent assay and Northern hybridization, we characterized the Harosoy resistance and found that CMV-SC did not spread systemically but was restricted to the inoculated leaves in Harosoy. Harosoy resistance was not controlled by either a dominant or recessive single gene. To dissect this system controlling long-distance movement of CMV in soybean, we constructed infectious cDNA clones of CMV-SC and CMV-SD. Using these constructs and the chimeric RNAs, we demonstrated that two viral components were required for systemic infection by the virus. The region including the entire 2b gene and the 5' region of RNA3 (mainly the 5' untranslated region) together were required. By quantitative trait locus (QTL) analysis using an F(2) population and the F(3) families derived from Harosoy and susceptible 'Nemashirazu', we also showed that at least three QTLs affected systemic infection of CMV in soybean. Our study on Harosoy resistance to CMV-SC revealed an interesting mechanism, in which multiple host and viral genes coordinately controlled viral systemic infection.  相似文献