Relationship between antigen release and antibody response of infectious coryza water-in-oil-in-water emulsion vaccines     

Fukanoki S  Matsumoto K  Mori H  Takeda R 《Avian diseases》2000,44(4):869-873

The relationship between the antigen release from formulations in vitro and the antibody response after administration of water-in-oil-in-water (W/O/W) emulsion vaccines containing Haemophilus paragallinarum (Hpg) was studied in chickens. Increases of sorbitan sesquioleate volume in the formulation led to slower antigen release and tended to induce higher hemagglutination-inhibition (HI) antibody titers. In addition, the vaccines prepared with internal aqueous phase:oil phase:external aqueous phase (A:O:A) ratios of 3:4:3 and 3:3:4 also showed slower release of antigen and higher HI antibody titer compared with those of an A:O:A ratio of 3:2:5. Vaccines prepared with polyoxyethylene (POE)(10) hydrogenated castor oil or POE(40) hydrogenated castor oil instead of sorbitan sesquioleate showed higher release and lower antibody HI titers. As a result, HI antibody titers at 6 wk after vaccination were inversely related to antigen release, as determined by the release test. The correlation coefficient was 0.942. In infectious coryza W/O/W emulsion vaccines, the slow release of antigen from the formulation induced and maintained high HI antibody titers of Hpg.  相似文献   

Experimental reinfection with homologous porcine reproductive and respiratory syndrome virus in SPF pigs     

Shibata I  Mori M  Yazawa S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2000,62(1):105-108

The present examination was conducted to determine if the pigs infected with one strain of porcine reproductive and respiratory syndrome virus (PRRSV) would be protected against a subsequent homologous virus challenge. Sixteen 4-week-old SPF pigs were assigned to 2 experimental groups A and B. The pigs in group A were inoculated with 10(6.5) TCID50 of PRRSV by intranasal route. On 77 days post-inoculation (PI), pigs in groups A and B were similarly inoculated with same virus. After the secondary inoculation, the pigs in group A didn't show any clinical sign including pyrexia and reduction of white blood cell (WBC) number. Viremia was detected only on 3 days PI with low virus titer and any virus was not recovered from serum and tissues at the time of necropsy on 14 or 28 days PI. In contrast, pigs in group B showed pyrexia for 14 days and reduction of WBC number on 3 days PI. Viremia was detected between 3 and 28 days PI, and virus was isolated from several tissues of all pigs. These results indicate that previous exposure to PRRSV can prevent development of clinical signs and reduce virus proliferation in pigs after subsequent infection with the homologous PRRSV.  相似文献   

Sex determination based on fecal DNA analysis of the amelogenin gene in sika deer (Cervus nippon)     

Yamauchi K  Hamasaki S  Miyazaki K  Kikusui T  Takeuchi Y  Mori Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2000,62(6):669-671

A sex determination method using DNA extracted from feces has been developed for sika deer (Cervus nippon). We determined a partial sequence of the amelogenin gene of sika deer, which exists on both X and Y chromosomes with a deletion region on the Y chromosome. Based on the sexually dimorphic sequences, we designed a pair of primers which could amplify DNA fragments the lengths of which are different between males and females. PCR products were detected in 34 out of 37 fecal samples collected from captured deer and the sexes estimated by the present method were perfectly matched with the actual sexes.  相似文献   

Immunochromatographic assay for simple and rapid detection of <Emphasis Type="Italic">Satsuma dwarf virus</Emphasis> and related viruses using monoclonal antibodies     

Nario Kusano  Keita Hirashima  Minoru Kuwahara  Kenji Narahara  Tadashi Imamura  Tomohiro Mimori  Ken Nakahira  Kuniaki Torii 《Journal of General Plant Pathology》2007,73(1):66-71

A simple and rapid immunochromatographic assay (ICA) to detect Satsuma dwarf virus (SDV) was developed using colloidal gold conjugates of anti-SDV monoclonal antibodies. Of six homogenization buffers tested, 0.1 M citrate buffer (pH 7.0) gave the best results for the ICA. In the ICA, addition of 0.1% thioglycolic acid in the homogenization buffers that have been widely used in enzyme-linked immunosorbent assays (ELISA) was deleterious to the reaction because of undesirable coagulation of the colloidal gold. ICA using the anti-SDV monoclonal antibodies was 8 times and 16 times more sensitive than double antibody sandwich-ELISA and ICA using the anti-SDV polyclonal antibody, respectively. The analysis is complete in only 15 min. Furthermore, ICA using the anti-SDV monoclonal antibodies could also detect SDV-related viruses.  相似文献   

中国东方蜜蜂分类研究结果和进展   总被引：5，自引：0，他引：5  

糜佳霖  谭垦 《中国蜂业》2007,58(7):13-14,16

本文概述了中国东方蜜蜂分类研究的发展概况,并总结了对68个地区188个样点的东方蜜蜂进行多重分析方法对形态测定的数据得出的结果:中国大陆的东方蜜蜂主要分为4个类群:1.生活在吉林、辽宁、北京和山西的东方蜜蜂;2.甘肃天水、四川中部的体型较大的东方蜜蜂;3.云南南部、广东、广西、香港合海南的体型较小的东方蜜蜂;4.中国其它地区的东方蜜蜂。这一最新结果与分子标记鉴定的结果相一致。  相似文献   

Bioethics Between Pain and Welfare     

de Mori  B. 《Veterinary research communications》2007,31(1):65-71

Veterinary Research Communications - de Mori, B., 2007. Bioethics between pain and welfare. Veterinary Research Communications, 31(Suppl. 1), 65–71  相似文献   

Plectosporium blight of pumpkin and ranunculus caused by Plectosporium tabacinum     

Toyozo Sato  Tadaoki Inaba  Mitsutaka Mori  Ken Watanabe  Keisuke Tomioka  Etsuji Hamaya 《Journal of General Plant Pathology》2005,71(2):127-132

Based on inoculation experiments and morphological studies on the pathogenic isolates of Plectosporium spp., Microdochium blight (Hakuhan-byo in Japanese) of pumpkin (Cucurbita maxima Duch.) occurring in Japan was reconfirmed to be caused by Plectosporium tabacinum, and seedling blight (Kabugare-byo in Japanese) of ranunculus (Ranunculus asiaticus L.) was demonstrated to be caused by P. tabacinum. Both diseases are renamed Plectosporium blight in this article. Some isolates of the fungus appeared to have host specificity, whereas the others had either weak or no pathogenicity to pumpkin and ranunculus.  相似文献   

The impact of controlled release capsules of monensin on postcalving haptoglobin concentrations in dairy cattle          下载免费PDF全文

Rex G. Crawford  Ken E. Leslie  Randal Bagg  C. Paul Dick    Todd F. Duffield 《Canadian journal of veterinary research》2005,69(3):208-214

This study was designed to assess the impact of a controlled release capsule (CRC) of monensin, administered prior to calving, on postcalving haptoglobin levels. The role of disease on haptoglobin levels was also studied. The study population consisted of 1010 cows from 25 Holstein dairy herds near Guelph, Ontario. Monensin CRC or placebo capsules were randomly assigned within each herd 3 wk prior to the expected calving date. Serum from week 1 and week 6 postcalving was submitted for quantification of haptoglobin concentrations. Haptoglobin results were analyzed for associations with treatment, health data, and individual cow factors up to 95 d in milk. Haptoglobin concentrations were higher in week 1 than week 6 (P < 0.05). In univariate analysis, several diseases were significantly associated with haptoglobin concentrations. However, occurrence of disease appeared to be a confounding factor in the data interpretation. Thus, the analysis was stratified by the presence or absence of disease. There appeared to be associations between factors other than clinical disease contributing to increased haptoglobin levels in both clinically healthy and unhealthy cattle. Haptoglobin served as a good indicator of inflammatory disease. Monensin CRC treatment was associated with increased haptoglobin concentrations in clinically unhealthy cattle, perhaps reflecting a better ability to respond to disease challenge. The lower haptoglobin concentrations in monensin CRC treated cattle that were clinically normal may be a reflection of reduced subclinical disease.  相似文献   

Hog Cholera: III. Investigation of the Complement-Fixation Test for the Detection of the Virus in Swine Tissue   总被引：1，自引：1，他引：0       下载免费PDF全文

P. Boulanger  M. Appel  G. L. Bannister  Gerda M. Ruckerbauer  K. Mori    D. P. Gray 《Canadian journal of veterinary research》1965,29(8):201-208

The complement-fixation test was investigated as a means of detecting hog cholera virus in spleen from experimentally infected swine. Various methods of extracting the tissue for production of antigen are described and emphasis is placed on the necessity of using the modified direct complement-fixation test to obtain reactions. The tissue should be obtained from animals showing advanced clinical manifestations of the disease. Preferably, the tissue should be maintained frozen or at least well refrigerated. The results indicate that tissue from dead animals or from breeding sows should be avoided. The 77 per cent positive reactions obtained suggest the test could be of diagnostic value provided two or three samples are obtained from the same herd.  相似文献   

南瓜的价值及抗逆栽培生理研究进展   总被引：21，自引：0，他引：21  

郭文忠  李锋  秦垦  吴晓刚 《长江蔬菜》2002,(9):30-32

综述了南瓜的栽培、营养及医疗保健价值，并对相关生理栽培研究进行了介绍。  相似文献