Peroxidase gene-based estimation of genetic relationships and population structure among Citrus spp. and their relatives     

Aydin Uzun  Osman Gulsen  Ubeyit Seday  Turgut Yesiloglu  Yildiz Aka Kacar 《Genetic Resources and Crop Evolution》2014,61(7):1307-1318

Many stress-related interactions such as pathogen infection, insect tolerance, salt tolerance, auxin degradation, cell wall lignification, tissue suberization, and plant senescence involve various isoforms of peroxidases. Peroxidase gene polymorphism (POGP) markers have been used to estimate diversity, relationships and population structure among 80 Citrus and their relatives in Aurantioideae by using unweighted pair group method arithmetic average (UPGMA) and bayesian substructuring analysis. Fourteen primers produced a total of 148 fragments and 147 of them were polymorphic. The UPGMA analysis demonstrated that the accessions had a similarity range from 0.27 to 0.98 and were distinguished. The results of this study were mostly consistent with previous reports of different marker systems, but few different findings were also detected. The subtribe Clauseninae (tribe Clauseneae) did not clearly separate from the subtribes of the tribe Citreae. Substructuring analysis indicated that there were six subpopulations among the accessions studied. This study revealed that the POGP markers can be utilized to estimate genetic diversity, relationships and population structure in Citrus and related species in the Aurantioideae subfamily.  相似文献   

Investigation of Bartonella henselae in cats in Ankara, Turkey     

Celebi B  Kilic S  Aydin N  Tarhan G  Carhan A  Babur C 《Zoonoses and public health》2009,56(4):169-175

The purpose of this study was to determine Bartonella henselae prevalance in cats in Ankara. Whole bloods and sera collected from 256 cats were investigated for the presence feline Bartonella species by culture and sera were tested for the presence of antibodies against B. henselae IgG using immunofluorescence assay. Bartonella species were isolated by blood culture from 24 (9.4%) cats. Bartonella isolates were subjected to restriction fragment length polymorphism (RFLP) by using TaqI and HhaI endonucleases to identify species. Twenty-one isolates were determined as B. henselae and three of 24 isolates were determined as Bartonella clarridgeiae with RFLP. The bacteraemia prevalence and seroprevalence of B. henselae IgG antibodies in cats was detected as 8.2% and 18.6% respectively. This is the first report on B. henselea and B. clarridgeiae in cats in Turkey.  相似文献   

Peroxisome proliferator-activated receptor (PPAR) expression in cultured bovine endometrial cells and response to omega-3 fatty acid, growth hormone and agonist stimulation in relation to series 2 prostaglandin production     

MacLaren LA  Guzeloglu A  Michel F  Thatcher WW 《Domestic animal endocrinology》2006,30(3):155-169

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Effects of thiacloprid, deltamethrin and their combination on oxidative stress in lymphoid organs, polymorphonuclear leukocytes and plasma of rats     

Birsen Aydin 《Pesticide biochemistry and physiology》2011,100(2):165-171

Deltamethrin and thiachloprid are an α-cyano class pyrethroid and neonicotinoid insecticide, respectively. Recently, a pesticide combining deltamethrin and thiacloprid has also been released. In the present study, the acute and subacute toxic effects of deltamethrin, thiachlopride, and a combination of these insecticides, on the lymphoid organs (spleen, thymus and bone marrow), polymorphonuclear leukocytes (PMNs) and plasma of rats, were determined to better understand mammalian antioxidant-oxidant and inflammatory system responses. For this purpose, rats were treated orally with different doses of thiacloprid (single acute dose of 112.5 mg/kg); subacute dose of 22.5 mg/kg/day for 30 days; deltamethrin (single acute dose of 15 mg/kg); subacute dose (3 mg/kg/day for 30 days), or a combination of these pesticides. Results were compared with those from a comparable dosing regimen with the known immunosuppressive drug cyclophosphamide. Pesticide treatments caused significant changes in the levels of liver and kidney injury markers. Antioxidant enzyme (catalase and glutathione peroxidase), glutathione and plasma antioxidant levels decreased but lipid peroxidation increased in all lymphoid organs and the plasma. Glutathione-S-transferase and especially DT-diaphorase activity, decreased after thiacloprid treatment. Myeloperoxidase activity, carbonyl content, lipid peroxidation and total nitrite levels increased in PMNs and plasma. When evaluated as a whole, the oxidative and inflammatory stresses seen in the pesticide combination groups were not much more pronounced than in the groups treated with a single pesticide. In terms of the evaluated biochemical parameters, the pesticides showed similar effects to cyclophosphamide.  相似文献   

鸡减蛋综合症病毒（EDS—76）的分离与鉴定     

张福萍  吐尔逊 《新疆农业大学学报》1997,20(1):47-49

采取新疆五家渠某鸡场发病鸡病料，接种鸭胚，分离病毒，病料在鸭胚中盲传至第５代，鸭胚尿囊液ＨＡ效价达２１７，ＨＡ可被ＥＤＳ－７６标准阳性血清抑制。免疫电镜观察到了病毒颗粒，表明该病是由ＥＤＳ－７６病毒所致  相似文献   

Hail detection with a differential reflectivity radar     

Bringi VN  Seliga TA  Aydin K 《Science (New York, N.Y.)》1984,225(4667):1145-1147

A major objective in the remote sensing of convective storms by radar is the clear and reliable differentiation between regions of hail and regions of rainfall. This report describes the application of the differential reflectivity (Z(DR)) radar technique to the problem of hail detection. The procedure is based on the markedly different polarization-dependent backscatter characteristics of rain and hail. Field experiments conducted in Colorado during the spring of 1983 provide significant evidence of hail detection with this new radar technique.  相似文献   

Boron uptake and toxicity in maize genotypes in relation to boron and phosphorus supply     

Aydin Günes  Mehmet Alpaslan 《Journal of plant nutrition》2013,36(4):541-550

The effect of increasing levels of applied phosphorus (P) and boron (B) on the growth and the concentration and uptake of P and B were examined in eight maize genotypes (Furio, Riogrande, Sele, DK 743, Helix, Missouri, Betor, and Poker) with two individual greenhouse experiments. Phosphorus was applied at 0, 50, and 100 mg kg^‐1 in Experiment I and B at 0, 10, and 30 mg kg^‐1 levels in Experiment II. Application of B resulted in increased concentration and uptake of B and a decreased P concentration and uptake in all genotypes. The dry weight of all genotypes was decreased by application of B. However, applied P decreased B concentration and uptake and increased P concentration and uptake of the genotypes. The dry weight of all genotypes was increased by application of P. These experiments show that B is more toxic in the absence of rather than the presence of P, and that this toxicity could be alleviated with applications of P in the calcareous soils of semiarid areas.  相似文献   

Interspecific Facilitative Root Interactions and Rhizosphere Effects on Phosphorus and Iron Nutrition Between Mixed Grown Chickpea and Barley     

Aydin Gunes  Esra G. Bagci  Ali Inal 《Journal of plant nutrition》2013,36(9):1455-1469

A glasshouse study was conducted to investigate the effects of interspecific complementary and competitive root interactions and rhizosphere effects on phosphorus (P) and iron (Fe) nutrition of mixed grown chickpea (Cicer arietinum L.) and barley (Hordeum vulgare L.). In order to provide more physiological evidence on the mechanisms of interspecific facilitation, we determined phosphatase activities in plant and rhizosphere, and root ferric reducing capacity (FR), Fe-solubilizing activity (Fe-SA) and rhizosphere pH. The results of the experiment revealed that biomass yield of barley was significantly increased by associated chickpea as compared to monocultured barley, while mixed cropping caused a slight decreases in the biomass yield of chickpea. The rhizosphere was strongly acidified under chickpea and chickpea/barley mixed cropping and this acidification, in turn, increased the available P, Fe(II) and DTPA-Fe concentrations in the rhizosphere. Fe-solubilizing activity (Fe-SA) and ferric reducing (FR) capacity of the roots were higher in both species grown in mixed culture relative to their monoculture which may improve Fe nutrition of both species. Leaf acid phosphatase (APase, EC 3.1.3.2) activity was higher in both plants under mixed cropping that may improve P nutrition of barley by chickpea.  相似文献   

Effect of salinity on stomatal resistance,proline, and mineral composition of pepper     

Aydin Günes  Ali Inal  Mehmet Alpaslan 《Journal of plant nutrition》2013,36(2):389-396

Pepper (Capsicum annuum L.) plants grown in pots were irrigated with the nutrient solutions containing 50, 75, and 100 mM NaCl or a control solution. Salinity markedly decreased plant growth. Increasing salinity levels increased stomatal resistance and sodium (Na), chloride (Cl), proline contents of the plants. Potassium (K), total‐nitrogen (N), and chlorophyll content of the plants were decreased under high salinity conditions.  相似文献   

A microsporidian pathogen of the predatory beetle Rhizophagus grandis (Coleoptera: Rhizophagidae)     

Yaman M  Radek R  Weiser J  Aydin C 《Folia parasitologica》2010,57(3):233-236

A new Microsporidium sp. infects Rhizophagus grandis Gyllenhall, a beetle which preys on the bark beetle Dendroctonus micans Kugellan in Turkey. Mature spores are single, uninucleate, oval in shape (3.75 +/- 0.27 microm in length by 2.47 +/- 0.13 microm in width), with a subapically fixed polar filament. The polar filament is anisofilar, coiled in 7-8 normal and 3-4 reduced coils. Other characteristic features of the microsporidium are the four/five nuclear divisions to form 16/32 (commonly 16) spores, subpersistent sporophorous vesicles (pansporoblasts) remaining till formation of the endospore, and the vesicles dissolved with free mature spores. The polaroplast is divided into three zones: an amorphous zone, dense layers, and a lamellartubular area extending to the central part of the spore.  相似文献