Evaluation of Direct In Vivo Gene Transfer in an Equine Metacarpal IV Ostectomy Model Using an Adenoviral Vector Encoding the Bone Morphogenetic Protein‐2 and Protein‐7 Gene     

Christopher E. Kawcak DVM  PhD  Diplomate ACVS  Chisa Hidaka MD  C. Wayne Mcilwraith BVSc  PhD  Diplomate ACVS  Natasha Werpy DVM  Diplomate ACVR  Jennifer Macleay DVM  PhD  Diplomate ACVIM  David D. Frisbie DVM  PhD  Diplomate ACVS 《Veterinary surgery : VS》2012,41(3):345-354

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Expression of NOS and VEGF in feline mammary tumours and their correlation with angiogenesis     

Islam MS  Matsumoto M  Hidaka R  Miyoshi N  Yasuda N 《Veterinary journal (London, England : 1997)》2012,192(3):338-344

In order to define the role of nitric oxide (NO) in feline mammary tumours, the expression of endothelial or inducible nitric oxide synthase (e/iNOS) and vascular endothelial growth factor (VEGF), and their relationship with angiogenesis, was investigated in 23 feline mammary tumours (two hyperplastic, 19 adenocarcinoma, one osteosarcoma and one squamous cell carcinoma) by immunohistochemistry. Tumour angiogenesis was assessed by CD31 immunostaining and was expressed as microvessel density (MVD). In general, iNOS immunoreactivity was localised in tumour cells and occasionally in stromal myofibroblasts, whereas eNOS and VEGF were localised in the cytoplasm of tumour epithelial cells and endothelium. In malignancy, expression of iNOS increased from well- to less-differentiated phenotypes (Grades 1-3) and was significantly higher in G3 and G2 when compared with G1 cases. However, increasing eNOS expression was limited only in hyperplastic lesions and showed no significant changes among the grades. In addition, expression of iNOS was positively correlated with VEGF and MVD in feline mammary tumours and both measures were significantly greater in less differentiated phenotypes (P<0.05). In conclusion, the expression of NOS isoforms in feline mammary tumours depended on tumour grade, and the positive correlations between iNOS and angiogenic markers suggests that iNOS synthesised by tumour cells promotes tumour growth. Thus, iNOS can be used as an important immunohistochemical marker to determine the degree of malignancy and prognosis of feline mammary carcinoma.  相似文献   

Comparison of morphological and DNA-based techniques for stomach content analyses in juvenile chum salmon <Emphasis Type="Italic">Oncorhynchus keta</Emphasis>: a case study on diet richness of juvenile fishes     

Sakiko Orui Sakaguchi  Shigeru Shimamura  Yuichi Shimizu  Gen Ogawa  Yuichiro Yamada  Keiko Shimizu  Hiroaki Kasai  Hiroshi Kitazato  Yoshihiro Fujiwara  Katsunori Fujikura  Kiyotaka Takishita 《Fisheries Science》2017,83(1):47-56

To identify the stomach contents of marine organisms, morphological observations are commonly conducted. However, the results obtained by this traditional method are frequently biased, as it is difficult to detect partially digested, soft-bodied organisms. To resolve this, recent studies have used DNA-based (DNA barcoding) analyses to examine the diet breadth of marine organisms. Here, we compared the prey richness of juvenile fishes using morphological observations and DNA-based analyses, with a focus on juvenile chum salmon Oncorhynchus keta. A higher number of prey taxa were successfully identified using DNA-based analyses than morphological observations. However, we also noticed several shortcomings of the DNA-based analyses, as reported in other diet-analysis studies that used molecular techniques. For example, the degree of digestion among prey taxa might have resulted in differential sensitivity to DNA detection. Additionally, several prey taxa could not be precisely identified, as the sequence data for some of the targeted organismal groups are unavailable in public gene databases. Remarkably, it is also possible that DNA-based analyses detected secondary prey, and therefore, the richness of prey taxa was likely overestimated. Thus, dietary analyses of juvenile fishes need to be carefully conducted, considering the respective advantages and disadvantages of DNA-based and morphological techniques.  相似文献   

Molecular detection of protists associated with the Manila clam Ruditapes philippinarum inhabiting the Wajiro tidal flat in Hakata Bay,Fukuoka Prefecture     

Takishita  Kiyotaka  Kawai  Rihoko  Tsutsumi  Ayaka  Tanifuji  Goro  Otsubo  Mayuko 《Fisheries Science》2020,86(4):633-643

Fisheries Science - Tidal flats are ecologically important as they support a large community of animals (e.g., crabs, mollusks, and polychaetes) and restore water quality. However, information...  相似文献   

A putative TetR-type transcription factor AZC_3265 from the legume symbiont Azorhizobium caulinodans represses the production of R-bodies that are toxic to eukaryotic cells     

Jun-ichi Matsuoka  Fumiko Ishizuna  Keigo Kurumisawa  Kengo Morohashi  Tetsuhiro Ogawa  Makoto Hidaka 《Soil Science and Plant Nutrition》2013,59(5):452-459

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Deoxyribonucleic Acid Homology Among Strains of Soybean-Nodulating Bacteria     

Kiyotaka Miyashita 《Soil Science and Plant Nutrition》2013,59(4):639-643

Abstract

Characteristics of Brown Forest soils developed under different bio-climatic conditions from low to high eleyations in the Kinki District were studied with special reference to their pedogenetic processes. The Brown Forest soils at high elevations were characterized by a lower bulk density, higher capacities to adsorb organic matter, phosphate, and moisture, which were correlated with the ratio of the amorphous content to the clay content (the value of the ratio of (Feo + Alo)/clay), as compared to those at low elevations. Considering the fact that the value of the (Feo + Alo)/clay ratio was not correlated with the volcanic glass index, the formation of an amorphous fraction at high elevations was considered to proceed according to the following mechanism.

Low temperature at high elevations (above 700 m) may retard the crystallization of oxide minerals. The amorphous oxides with variable positive charge thus formed may adsorb organic matter, confering a darker color and high moisture and high phosphate retention capacities to the subsoil. Adsorbed organic matter stabilizes these amorphous oxides, thus enhancing the amorphous properties and inhibiting crystallization. A1 translocation due to the weak podzolization may contribute to the increase of the content of amorphous materials.

Characterization of the B horizons in forest soils in Japan, in terms of the values of the ratios of (Feo + Alo)/clay, (Fed-Feo)/Fet, and Alo/Ald, (CEC -ECEQ/CEC and STPT-ZPC, suggested that forest soils might be classified into four groups.  相似文献   

Nocturnal/diurnal demand-feeding pattern of yellowtail Seriola quinqueradiata under different keeping conditions   总被引：1，自引：0，他引：1  

Jun Kohbara  Iwao Hidaka  Isao Kuriyama  Mitsushi Yamashita  Masasuke Ichikawa  Kiyoshi Furukawa  Katsumi Aida  F. Javier Sánchez-Vázquez and  Mitsuo Tabata 《Fisheries Science》2000,66(5):955-962

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Response changes with growth in the gustatory receptors of young yellowtails (Seriola quinqueradiata)     

Jun Kohbara  Kiyoshi Furukawa  Hajime Taishaku  Iwao Hidaka 《Fisheries Science》2000,66(6):1106-1114

SUMMARY: Laboratory-hatched juvenile yellowtails (mean fork length, 9.3 cm) were reared for 2 months in two groups on different kinds of food, mysid and squid in one group and fish in the other, and the gustatory neural responses to seven chemicals (adenosine-5'-monophosphate, alanine, arginine, betaine, proline, tryptophan, and valine) were recorded in fish before and after the 2-month rearing period. Large variances in the responses to some chemicals were noticed in the juveniles before the rearing experiment. Mann–Whitney U -tests on the neural responses indicated significant changes in the magnitude of the responses to valine and some other chemicals relative to that for proline or tryptophan between the pre- and the two post-rearing groups. No significant differences in the response magnitude for the seven chemicals were observed for the two post-rearing groups suggesting that the response changes during the 2-month rearing might have been intrinsic and not due to specific food items in the diet. The dose–response relationship for some chemicals was also examined in the juveniles before and after the 2-month rearing. A slight lowering of threshold was noticed for alanine and valine after the rearing. Data on the responses of wild yellowtails were in support of the changing responsiveness of gustatory receptors during development of juvenile yellowtails.  相似文献   

Occurrence of orally administered mulberry 1-deoxynojirimycin in rat plasma     

Nakagawa K  Kubota H  Kimura T  Yamashita S  Tsuzuki T  Oikawa S  Miyazawa T 《Journal of agricultural and food chemistry》2007,55(22):8928-8933

1-deoxynojirimycin (DNJ), a potent glucosidase inhibitor, is a characteristic constituent of the mulberry leaf. Dietary mulberry DNJ may be beneficial for the suppression of abnormally high blood glucose levels, thereby preventing diabetes mellitus. Although there is considerable interest in the effects of mulberry DNJ, the intestinal absorption and pharmacokinetic profile of orally administered mulberry DNJ have never been characterized. In this study, we developed a method for determining the level of plasma DNJ by hydrophilic interaction chromatography coupled to a mass spectrometric detector (HILIC-MS) to investigate the absorption and metabolism of orally administered mulberry DNJ in rats. DNJ was separated from plasma extract on a TSK gel Amide-80 column, a representative column for HILIC. At postcolumn, DNJ was concurrently detected and identified by MS. The plasma DNJ concentration in fasted rats was below the detection limit [<1 microg (6 nmol)/mL]; however, the concentration reached a maximum [15 microg (92 nmol)/mL] 30 min after the administration of mulberry DNJ (110 mg/kg of body weight), and the DNJ concentration decreased rapidly thereafter. When the rats received different amounts of mulberry DNJ (1.1, 11, and 110 mg/kg of body weight), dose-dependent incorporation of DNJ into the plasma was confirmed. We did not detect any DNJ metabolites in the plasma. These findings indicate that orally administered mulberry DNJ is absorbed as an intact form from the alimentary tract and then is quickly excreted from the body. The developed HILIC-MS method could be applied in determining levels of DNJ in urine and tissues, and therefore, the method would be a powerful tool for studying the metabolic fate of mulberry DNJ as well as its bioavailability.  相似文献   

Serum lactoferrin and immunoglobulin G concentrations in healthy or ill neonatal foals and healthy adult horses     

Barton MH  Hurley D  Norton N  Heusner G  Costa L  Jones S  Byars D  Watanabe K 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2006,20(6):1457-1462

BACKGROUND: Lactoferrin is a colostral glycoprotein with antimicrobial properties. HYPOTHESES: (1) Serum lactoferrin and immunoglobulin G (IgG) concentrations are correlated and increase in healthy foals after ingestion of colostrum; (2) compared to healthy foals, ill foals will have lower lactoferrin concentrations that correlate with their IgG concentration, neutrophil count, the diagnosis of sepsis, and survival; and (3) plasma concentrations of lactoferrin will be less than serum concentrations. ANIMALS: Healthy foals (n = 16), mature horses (n = 10), and ill foals 1-4 days old (n = 111) that were examined for suspected sepsis were used for blood collection. Colostrum was obtained from 10 healthy mares unrelated to the foals. METHODS: Blood was obtained from the healthy foals at birth and 1-3 days of age and from the ill foals at admission. Serum IgG was quantified by single radial immunodiffusion (SRID). Lactoferrin concentrations in colostrum and blood were determined by an enzyme-linked immunosorbant assay. The sepsis score, blood culture results, neutrophil counts, and survival were obtained on ill foals. RESULTS: The mean colostral lactoferrin concentration was 21.7 microg/mL. Compared to values at birth, serum IgG (18+/-2 versus 2,921+/-245 mg/dL, SEM) and lactoferrin (249+/-39 versus 445+/-63 ng/mL, SEM) concentrations were significantly greater in healthy foals 1-3 days old. Serum lactoferrin concentration in 1-3-day-old healthy foals was not different from mature horses or ill foals. IgG and lactoferrin concentrations were significantly correlated only in healthy foals. Serum lactoferrin concentrations were significantly lower in ill neutropenic foals. The serum IgG concentration was significantly lower in ill foals as compared to healthy foals. Only serum IgG was significantly less in ill foals with a positive sepsis score and in nonsurvivors, Plasma lactoferrin concentrations were lower than serum concentrations, although values were significantly correlated. CLINICAL IMPORTANCE: Although both serum IgG and lactoferrin concentrations increase in healthy foals after ingestion of colostrum, only serum IgG is significantly correlated with the sepsis score and outcome.  相似文献