Comment on "Single Crystals of Single-Walled Carbon Nanotubes Formed by Self-Assembly"     

Chisholm MF  Wang Y  Lupini AR  Eres G  Puretzky AA  Brinson B  Melechko AV  Geohegan DB  Cui H  Johnson MP  Pennycook SJ  Lowndes DH  Arepalli S  Kittrell C  Sivaram S  Kim M  Lavin G  Kono J  Hauge R  Smalley RE 《Science (New York, N.Y.)》2003,300(5623):1236; author reply 1236

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Isolation and characterization of Campylobacter,Helicobacter, and Anaerobiospirillum strains from a puppy with bloody diarrhea     

Misawa N  Kawashima K  Kondo F  Kushima E  Kushima K  Vandamme P 《Veterinary microbiology》2002,87(4):353-364

We carried out a microscopic examination of stools from a 2-month-old female puppy with bloody diarrhea, and this revealed large numbers of different spiral-shaped bacteria. To isolate these organisms, a rectal swab specimen was inoculated onto plates of Skirrow's agar and incubated at 37 degrees C for 6 days in a microaerobic atmosphere. Finally, a total of six different spiral-shaped bacteria (strains G1104, 94105, FR106, B0101, 3J102, and J2103) were isolated. Based on their morphology, biochemical traits, whole-cell protein profiles, and analysis of their 16S rDNA sequences, they were identified as Campylobacter upsaliensis, Helicobacter cinaedi, 'Flexispira rappini', two Anaerobiospirillum spp. with different morphologies, and Helicobacter sp., respectively. Analysis of 16S rRNA gene sequence data for strains 94150 (H. cinaedi) and FR106 (F. rappini) revealed that this approach has limitations when identifying isolates to the species level because of a high degree of sequence homology between these species (>99%) and considerable sequence variation among different isolates within these species. The dog was treated orally with amoxicillin for 3 days, which resolved the diarrhea. However, 1 day after the last dose the bloody diarrhea recurred but regarded to six more days amoxicillin treatment. This suggests a bacterial cause for the diarrhea. The approach to identification to microaerobic spiral-shaped bacteria in diarrheic dogs can be applied further to characterize their role in diarrhea illness.  相似文献   

The effect of wind force with reference to lodging of paddy rice     

Michiyoshi Kono  Jisuke Takahashi 《Soil Science and Plant Nutrition》2013,59(6):20-27

The lodging of crops is usually caused by wind and/or rain. However, few have studied the influence of wind force in relation to lodging. Grafius (1) blew wind of about 2.6 meters per second on barley plants and oats, and discovered that the moment of force per square centimeter on the leaves and culms was approximately one-fourth that on the heads.  相似文献   

Further evidence for the integrity of rice chloroplasts isolated in glutaraldehyde medium     

Koji Morita  Michiyoshi Kono 《Soil Science and Plant Nutrition》2013,59(4):317-320

Morphological aspects of rice chloroplasts isolated in glutaraldehyde and sucrose media were studied using the electron microscope. Chloroplasts in glutaraldehyde medium contained compact lamellae and densely stained stroma. On the other hand, chloroplasts in sucrose medium lacked stroma and consisted mostly of lamellae systems.  相似文献   

Elemental affinity for siderite found in a Japanese paddy subsoil     

Kazuki Togami  Kenzo Miura  Kumiko Ito  Hitoshi Kanno  Tadashi Takahashi 《Soil Science and Plant Nutrition》2013,59(2):101-109

ABSTRACT

Siderite (FeCO₃), a type of carbonate mineral, is very occasionally recognized as a nodule in anoxic soils and sediments. During siderite formation, elements are expected to be accumulated or excluded between siderite and bulk soil. Therefore, we verified the affinity of 40 elements for siderite found in the gley horizon of a smectite-rich paddy field in northeastern Japan from the elemental concentration of the soil and the siderite dissolved in 2.88 mol L^?1 hydrochloric acid (10% HCl). The concentration ratio of the target element of the soil to the siderite was expressed by the product of the dimensionless R₀ value and ratio of titanium (Ti) concentration of the soil to the siderite (C_siderite /C_soil) = R₀ (Ti_siderite /Ti_soil). The affinity of each element for the siderite was analyzed by comparing the R₀ values. In comparison with Ti used as the reference element, P, Cr, Co and Mo were accumulated (R₀ > 1.0), and alkali metals, such as Li, Na and K, and chalcophile elements, such as Cu, Zn, Cd and Pb, tended to be equal or excluded from the siderite (R₀ ≦1.0). Of the group 2 elements, Ca, Sr and Ba were accumulated, while Mg was excluded. The affinity of Mn for siderite exceeded that of Fe as the main component of the siderite, which reflects the fact that siderite was formed in a fresh water condition. The siderite size did not influence the elemental affinity. Observation of micromorphology using a scanning electron microscope showed that the siderite nodules were aggregations of siderite particles and clays, suggesting that the siderite grew by taking in clay around it. The rare earth element (REE) distribution pattern showed a tendency not to increase linearly but to increase with forming a concave curve at the intervals of the four elements with the increase of atomic number (i.e. tetrad effects). Although these findings are from the lower horizons of anoxic soils, the elemental affinity for siderite of this study is useful in order to elucidate the complex elemental dynamics in the anoxic environment.  相似文献   

Sequencing of 16S−23S rRNA internal transcribed spacer and its application in the identification of Nocardia seriolae by polymerase chain reaction     

Tomoya Kono  Tsuyoshi Ooyama  Shih-Chu Chen  & Masahiro Sakai 《Aquaculture Research》2002,33(14):1195-1197

A method for the diagnosis of nocardiosis in yellowtail (Seriola quinqueradiata), using polymerase chain reaction (PCR), was developed in this study. Primers specific for Nocardia seriolae were synthesized based on the alignment of 16S?23S rRNA internal transcribed spacer region sequences of N. seriolae. The primers did not amplify specific PCR product from other fish pathogens. However, two and three fishes could be diagnosed as infected with N. seriolae by clinical signs and bacterial isolation. PCR amplification of N. seriolae by specific primers detected six infected fishes. Thus, the primers used in this study are useful in detecting nocardiosis in fish.  相似文献   

Analysis of expressed sequence tags from a yellowtail, Seriola quinqueradiata (Temminck & Schlegel), head kidney cDNA library     

Tomoya Kono  Kazuyoshi Okamoto  Masahiro Sakai 《Aquaculture Research》2002,33(4):291-297

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Solubilization of type I and V collagens in Japanese flounder muscle during chilled storage     

Takashi?Kimiya  Satoshi?KubotaEmail author  Michiya?Kono  Mitsuaki?Hirata  Haruhiko?Toyohara  Katsuji?Moriora  Yoshiaki?Itoh 《Fisheries Science》2005,71(3):672-678

ABSTRACT:   The post-mortem changes of type I and V collagens in Japanese flounder muscle during chilled storage were examined. The muscle softened significantly within 12 h under chilled storage. Both type I and V collagens isolated by ion-exchange chromatographies showed no remarkable changes in band patterns even after 24 h of storage, suggesting that degraded collagen molecules may be removed from the collagen fraction by a conventional preparation method. In contrast, type I and V collagen molecules were detected by Western blotting with each specific antibody in a saline extract and gradually increased during chilled storage. These results suggest that both type I and V collagens may participate in the post-mortem softening of fish muscle.  相似文献   

Basic characterization of avian NK‐lysin (NKL) from the Japanese quail,Coturnix japonica     

Taichiro Ishige  Hiromi Hara  Takashi Hirano  Tomohiro Kono  Kei Hanzawa 《Animal Science Journal》2014,85(1):90-95

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Erratum to: Effect of taurine supplementation on hepatic metabolism and alleviation of cadmium toxicity and bioaccumulation in a marine teleost,red sea bream, <Emphasis Type="Italic">Pagrus major</Emphasis>     

Takeshi Hano  Katsutoshi Ito  Kumiko Kono  Mana Ito  Nobuyuki Ohkubo  Kazuhiko Mochida 《Fish physiology and biochemistry》2017,43(3):911-912

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