Canine model of ischemic stroke with permanent middle cerebral artery occlusion: clinical and histopathological findings     

Byeong-Teck Kang  Jong-Hwan Lee  Dong-In Jung  Chul Park  Su-Hyun Gu  Hyo-Won Jeon  Dong-Pyo Jang  Chae-Young Lim  Fu-Shi Quan  Young-Bo Kim  Zang-Hee Cho  Eung-Je Woo  Hee-Myung Park 《Journal of veterinary science (Suw?n-si, Korea)》2007,8(4):369-376

The aim of the present study was to assess the clinical and histopathological findings in a canine model of ischemic stroke. Cerebral ischemic stroke was induced by middle cerebral artery occlusion in four healthy beagle dogs using silicone plugs. They showed neurological signs of forebrain dysfunction such as reduced responsiveness, head turning, circling, postural reaction deficits, perceptual deficits, and hemianopsia. These signs gradually regressed within 4 weeks without therapy. On magnetic resonance imaging, T2 hyperintensity and T1 hypointensity were found in the cerebral cortex and basal ganglia. These lesions were well-defined and sharply demarcated from adjacent brain parenchyma with a homogenous appearance. No abnormalities of the cerebrospinal fluid were observed. At necropsy, atrophic and necrotic lesions were observed in the cerebral cortex. The cerebral cortex, basal ganglia, and thalamus were partially unstained with triphenyl-tetrazolium chloride. Histopathologically, typical features of infarction were identified in cortical and thalamic lesions. This study demonstrates that our canine model resembles the conditions of real stroke patients.  相似文献   

Enhancement of protective immune responses by oral vaccination with Saccharomyces cerevisiae expressing recombinant Actinobacillus pleuropneumoniae ApxIA or ApxIIA in mice     

Sung Jae Shin  Seung Won Shin  Mi Lan Kang  Deog Yong Lee  Moon-Sik Yang  Yong-Suk Jang  Han Sang Yoo 《Journal of veterinary science (Suw?n-si, Korea)》2007,8(4):383-392

We previously induced protective immune response by oral immunization with yeast expressing the ApxIIA antigen. The ApxI antigen is also an important factor in the protection against Actinobacillus pleuropneumoniae serotype 5 infection; therefore, the protective immunity in mice following oral immunization with Saccharomyces cerevisiae expressing either ApxIA (group C) or ApxIIA (group D) alone or both (group E) was compared with that in two control groups (group A and B). The immunogenicity of the rApxIA antigen derived from the yeast was confirmed by a high survival rate and an ApxIA-specific IgG antibody response (p < 0.01). The highest systemic (IgG) and local (IgA) humoral immune responses to ApxIA and ApxIIA were detected in group E after the third immunization (p < 0.05). The levels of IL-1β and IL-6 after challenge with an A. pleuropneumoniae field isolate did not change significantly in the vaccinated groups. The level of TNF-α increased in a time-dependent manner in group E but was not significantly different after the challenge. After the challenge, the mice in group E had a significantly lower infectious burden and a higher level of protection than the mice in the other groups (p < 0.05). The survival rate in each group was closely correlated to the immune response and histopathological observations in the lung following the challenge. These results suggested that immunity to the ApxIA antigen is required for optimal protection.  相似文献   

In vitro neuronal and osteogenic differentiation of mesenchymal stem cells from human umbilical cord blood     

Ki-Soo Park  Yong-Soon Lee  Kyung-Sun Kang 《Journal of veterinary science (Suw?n-si, Korea)》2006,7(4):343-348

Mesenchymal stem cells (MSCs) have the capabilities for self-renewal and differentiation into cells with the phenotypes of bone, cartilage, neurons and fat cells. These features of MSCs have attracted the attention of investigators for using MSCs for cell-based therapies to treat several human diseases. Because bone marrow-derived cells, which are a main source of MSCs, are not always acceptable due to a significant drop in their cell number and proliferative/differentiation capacity with age, human umbilical cord blood (UCB) cells are good substitutes for BMCs due to the immaturity of newborn cells. Although the isolation of hematopoietic stem cells from UCB has been well established, the isolation and characterization of MSCs from UCB still need to be established and evaluated. In this study, we isolated and characterized MSCs. UCB-derived mononuclear cells, which gave rise to adherent cells, exhibited either an osteoclast or a mesenchymal-like phenotype. The attached cells with mesenchymal phenotypes displayed fibroblast-like morphologies, and they expressed mesenchym-related antigens (SH2 and vimentin) and periodic acid Schiff activity. Also, UCB-derived MSCs were able to transdifferentiate into bone and 2 types of neuronal cells, in vitro. Therefore, it is suggested that the MSCs from UCB might be a good alternative to bone marrow cells for transplantation or cell therapy.  相似文献   

架面不同部位对无核剂处理木纳格葡萄无核化效果的影响   总被引：2，自引：0，他引：2  

伍新宇  廖康 《北方园艺》2006,(4):47-49

用无核剂在花前、花后两次浸渍白木纳格和红木纳格葡萄花序和果穗,结果表明,架面各部位的花期没有明显差异,红木纳格花期更集中.架面前部的白木纳格、红木纳格可获得98.7%、93.8%的无核果率和7.36g、7.41g的果实粒重,架面中后部的果实无核果率、果粒重等品质有不同程度的下降.  相似文献   

Enriched production of N-hydroxycinnamic acid amides and biogenic amines in pepper (Capsicum annuum) flowers     

Sei Kang  Kyoungwhan Back 《Scientia Horticulturae》2006

The biosynthesis of N-hydroxycinnamic acid amides (HCAAs), such as feruloyltyramine, p-coumaroyltyramine, feruloylserotonin, and p-coumaroylserotonin, in pepper plants was investigated in view of the activity of their corresponding enzymes and substrate levels. Almost all HCAAs were found in flowers, whereas the enzyme activity essential for HCAA biosynthesis seemed to be expressed constitutively in all tissues tested. The highest levels of the biogenic amines tyramine and serotonin, the key amine substrates, were also found in flowers, indirectly suggesting that the amine substrates play an important regulatory role in the synthesis of HCAAs.  相似文献   

Exogenous carbon monoxide protects against the lung injury induced by ischemia-reperfusion of hind limbs in rats     

ZHOU Jun-lin  LING Yi-ling  SU Qing-jun  Kang Nan  LU Shi-bao  GUAN Li 《园艺学报》2006,22(2):303-306

AIM: To study the mechanism of protective effect of exogenous carbon monoxide (CO) in the lung injury induced by ischemia-reperfusion (IR) of hind limbs in rats. METHODS: Thirty-two SD rats were randomly divided into 4 groups: control, control+CO, IR and IR+CO. A rat model of ischemia in hind limbs and the reperfusion lung injury was made. The rats in IR+CO and control+CO groups were exposed to air containing 2.5×10-8 CO for 1 h before reperfusion or the corresponding control time point, while the other two groups were exposed to the routine air. The lung tissue structure, polymorphonuclear leukocyte (PMN) count, wet-to-dry weight ratio (W/D), malondialdehyde (MDA) content and the animal survival rate were observed. The carboxyhemoglobin (COHb) levels in artery blood were detected with CO-oximeter and the expression of intercellular adhesion molecule-1 (ICAM-1) in the lung was detected by Western blotting. RESULTS: Compared to control, the animal mortality, lung PMNs number, W/D, MDA content and ICAM-1 expression were all significantly increased in IR group. Compared with the IR group, the blood COHb level was significantly increased and the animal mortality, lung PMNs number, W/D, MDA content and ICAM-1 expression were all significantly decreased in IR+CO group. CONCLUSION: These data suggest that exogenous CO attenuate limb IR-induced lung injury by down-regulatiny ICAM-1 expression and suppressing PMN sequestration in the lung following limb IR in rats.  相似文献   

采后热空气处理对金冠苹果后熟衰老及病害的影响   总被引：2，自引：1，他引：2  

屠康  邵兴锋  赵艺泽 《果树学报》2006,23(4):562-567

金冠苹果采用38℃,72h或96h热空气处理,可降低0℃贮藏中果实的呼吸强度和乙烯释放量,延缓非溶性果胶的降解,维持较高的硬度、脆度,同时也会加速果皮的褪绿变黄和固酸比的上升,尤其是38℃96h处理的作用效果更为明显,显著提高了货架期后(20℃7d)苹果的质地品质和可接受程度,延缓衰老。苹果分别接种扩展青霉、灰葡萄孢霉,0℃和20℃条件下其病害发生率和腐烂均很严重,采用38℃96h热空气处理可以完全控制病害的发生,避免腐烂。可见,热空气处理一方面能延缓金冠苹果的后熟衰老,另一方面还能控制贮期病害的发生。  相似文献   

新型植物激素与蔬菜作物抗逆性关系研究进展   总被引：4，自引：0，他引：4  

康云艳  郭世荣  段九菊 《中国蔬菜》2007,1(5):39-39

综述了近年来水杨酸、茉莉酸类化合物、油菜素内酯、多胺和5-氨基乙酰丙酸等新型植物激素与蔬菜作物抗逆性关系的研究进展,从盐胁迫、低温胁迫、高温胁迫、低氧胁迫、病害胁迫、虫害胁迫等方面阐述和分析了新型植物激素与蔬菜作物抗逆性的相关性,并指出相关研究的有待深入之处。  相似文献   

沙棘植物柔性坝坝体变形初步分析   总被引：1，自引：0，他引：1  

李怀恩  张康  毕慈芬  乔旺林 《干旱区资源与环境》2007,21(3):144-148

本文基于2005年在内蒙古准格尔旗西召沟小流域对已植的沙棘柔性坝进行野外调查,从植物生长角度对沙棘柔性坝坝体进行了纵向、横向、垂向(即沙棘柔性坝坝长、坝宽、坝高)的变形分析。对于沙棘柔性坝的坝长变化,主要从沙棘根系蘖苗萌生出发,分析得出其每年扩展约1m;柔性坝坝宽受沟宽限制,其发展趋势为扩展至满沟;柔性坝坝高变化采用三次函数和Logistic型函数两种具有“S”形特征的曲线进行拟合分析,经对比其变化过程服从Logistic型生长函数,并结合Logistic函数对沙棘植物柔性坝坝高的变化进行了具体意义的探讨。  相似文献   

杂交稻对稻瘟病和稻白叶枯病的抗性鉴定   总被引：2，自引：0，他引：2       下载免费PDF全文

潘汝谦  徐大高  纪春艳  何汉生  康必鉴 《植物保护》2007,33(2):27-29

对广东省将推广的54份杂交稻组合对稻瘟病和白叶枯病的抗病性进行了鉴定。对稻瘟病的抗病性鉴定表明:抗病组合共49个,占90.7%;其中,高抗(抗性比≥91%)组合30个,占55.6%。对稻白叶枯病的抗病性鉴定表明:没有高抗(HR)和抗(R)的组合,仅有一个杂交稻组合(西胜2175)表现为中抗(mR),仅占1.9%,其余53个组合都表现感病,占98.1%。进一步分析表明:对稻瘟病表现高抗的30个杂交稻组合都不抗白叶枯病,而中抗白叶枯病的杂交稻组合西胜2175对稻瘟病表现为中抗,抗性比为74%。  相似文献