Molecular cloning,sequence characterization,and expression analysis of C-type lectin (CTL) and ER-Golgi intermediate compartment 53-kDa protein (ERGIC-53) homologs from the freshwater prawn,Macrobrachium rosenbergii     

Baliarsingh  Snigdha  Sahoo  Sonalina  Jo  Yong Hun  Han  Yeon Soo  Sarkar  Arup  Lee  Yong Seok  Mohanty  Jyotirmaya  Patnaik  Bharat Bhusan 《Aquaculture International》2022,30(2):1011-1035

Aquaculture International - Lectin protein families are diverse and multi-functional in crustaceans. The carbohydrate-binding domains (CRDs) of lectins recognize the molecular patterns associated...  相似文献   

Morphological and molecular variability among Indian isolates of <Emphasis Type="Italic">Rhizoctonia solani</Emphasis> causing banded leaf and sheath blight in maize     

Vimla Singh  Bimal S. Amaradasa  Chikappa G. Karjagi  Dilip K. Lakshman  Karambir S. Hooda  Aundy Kumar 《European journal of plant pathology / European Foundation for Plant Pathology》2018,152(1):45-60

Rhizoctonia solani, a devastating soil borne fungus inciting banded leaf and sheath blight (BLSB) disease is a constraint in maize production and improvement program. Rhizoctonia isolates collected from seven diverse maize cropping zones of India were examined for morphological and molecular variability. All the tested isolates caused symptoms of BLSB on maize and were also cross infective on rice and sugarcane hosts, but showed significant variability in hyphal diameter, mean hyphal cell size, weight, size and distribution of scleorotia, culture pigmentation, incubation period, pathogenicity and expression of symptoms. Neighbour joining cluster analysis placed the 62 isolates of R. solani into four major groups, A, B, C and D. Group A was more diverse and included isolates of diverse agro-ecological zones. The cluster analysis corresponded well with principle component analysis. Pathogenicity testing of R. solani isolates on maize genotype (CM 501) revealed highly variable virulence pattern of the pathogen population suggesting its high evolutionary potential, and hence adaptability to diverse geographical regions. The study reveals a strong evidence of inherent potential of the R. solani isolates to survive in diverse ecological zones and its probable spread to other maize cultivars across India. Sequence comparisons of the internal transcribed sequence-ribosomal DNA region of 62 isolates did not reveal much diversity among the isolates. Majority of the isolates (n?=?61) clustered together with anastomosis group (AG) AG1-IA used as reference strain in the phylogram, distinct from AG1-IB, AG2–2IIIB and Waitea circinata used as reference strains. BLSB isolates representing distinct geographical locations shared identical sequences indicating long-distance dispersal of the pathogen. The study confirms that the genetic flexibility of the pathogen allows for its adaptation to variable ecological niches and long-distance introduction of new genotypes into the region. The study emphasizes that epidemiological studies may complement the molecular studies.  相似文献   

Hemato-biochemical changes, disease incidence and live weight gain in individual versus group reared calves fed on different levels of milk and skim milk     

Lakshman K. BABU  Harnarain PANDEY  Ramesh C. PATRA  Artabandhu SAHOO 《Animal Science Journal》2009,80(2):149-156

A 2 × 3 factorial design was used to study the impact of rearing systems, individual (I) versus group (G) and different levels of milk/skim milk feeding (F1, F2 and F3) on hemato‐biochemical profile, disease incidence and average daily gain of crossbred (Bos indicus × Bos taurus) calves. Six calves were taken in each group on the basis of their birth weight and housed in individual (2.20 × 1.16 m²/calf) or in group pens (2.20 × 1.03 m²/calf). After 3 days of colostrum feeding, calves were allocated to one of three different milk feeding schedules: milk fed up to 8 weeks of age (F1), milk up to 4 weeks followed by 50% replacement by skim milk up to 6 weeks and 100% thereafter (F2) and 100% replacement of milk with skim milk after 4 weeks (F3). Calf starter and cereal green fodders were fed ad libitum from the second week of age and continued for 14 weeks. Parameters on health and disease profiles of calves (disease incidence, duration of illness, response to treatment and recovery) and weekly live weight change were recorded. Calf scour predominated (52.8%), followed by joint ill (25.0%) and respiratory infections (19.4%). The disease incidence was greater (P < 0.01) in individually housed calves (94.4 vs. 55.9%). The management of navel ill required longer recovery (7.01 days) followed by joint ill (4.87 days) and respiratory infection (4.86 days). The average daily gain during 0–14 weeks of age was higher (P < 0.01) in group‐housed calves (433 ± 22 vs. 355 ± 31 g), while the effect of feeding was not significant. Blood samples collected at 4, 8 and 14 weeks of age showed some periodic higher concentrations (but within normal range) of plasma urea and total protein in group housed calves on F2 and F3 feeding schedules in response to high protein intake. Other parameters remained non‐significantly different. Thus, group‐housed calves can be reared successfully with comparatively better performance and less illness than individually housed ones under the present health care and housing management system. However, the system should not be used as a substitute for good management, and frequent observations of calves should be an integral part of any successful rearing program.  相似文献   

Cloning, nucleotide sequence and phylogenetic analyses, and tissue-specific expression of the transferrin gene in Cirrhinus mrigala infected with Aeromonas hydrophila     

Sahoo PK  Mohanty BR  Kumari J  Barat A  Sarangi N 《Comparative immunology, microbiology and infectious diseases》2009,32(6):527-537

Transferrin partial complementary DNAs were cloned from the livers of five species in four genera of Indian carps (Indian major carp species: Labeo rohita, Catla catla and Cirrhinus mrigala; medium carp: Puntius sarana; minor carp: Labeo bata) subsequent to polymerase chain reaction amplification with published heterologous primers or self-designed primers derived from conserved regions of transferrin cDNA sequences. The partial transferrin cDNAs of the five species of carps had sizes from 624 to 633 bp (487 bp for L. rohita) and encoded an open reading frame consisting of 206–211 (162 for L. rohita) amino acids. The alignments of carp cDNA sequences showed 85–97% homology and 71–93% homology in deduced amino acid sequences. A phylogenetic tree of amino acid sequences of transferrin cDNAs from carps showed that the relationship among the four genera of Indian carps is well correlated with that derived from classic morphologic analyses. The hypothesized cleavage site and interdomain bridge of transferrin molecule were predicted for the above carp species and interestingly the cleavage site amino acid sequence was found to be conserved among all the carps. To study the tissue-specific expression of the transferrin gene, various tissues (liver, kidney, spleen, brain, muscle, testis, heart, intestine, gill and fin) from apparently healthy (control), moribund and survived C. mrigala experimentally infected with Aeromonas hydrophila infection were analyzed. Transferrin mRNA was detected only in liver RNA and to lesser extent in brain tissue out of the 10 tissues analyzed irrespective of bacterial infection.  相似文献   

天然梨竹林地上生物量及养分分配     

Pynskhem  K. Upadhyaya  U. K. Sahoo 《世界竹藤通讯》2012,10(4):48

2005~2006年间,对印度米佐拉姆天然梨竹林的地上生物量及养分分配进行了研究。根据不同成分对该竹种的立木生物量有很重要,可排序如下:竹秆 > 竹节 > 花序 > 叶 >。在NPK养分中,N的含量最高,然后是K,最后是P。每公顷总地上生物量随秆龄的增长而增加。在研究期间,发现与其他部位相比,叶的养分积累较少。这表明由于从生竹到开花,消耗了过多的养分,导致主要竹笋的死亡。  相似文献   

An improved and efficient micropropagation of Eclipta alba through transverse thin cell layer culture and assessment of clonal fidelity using RAPD analysis     

Shashi Kant Singh  Manoj K. Rai  Lingaraj Sahoo 《Industrial Crops and Products》2012,37(1):328-333

An improved and efficient in vitro regeneration system has been developed for Eclipta alba, a medicinally important plant, through transverse thin cell layer culture (tTCL). The transverse section of the nodal segment of field grown plants was used as tTCL explants for plant regeneration. Shoot multiplication from tTCL nodal explants was influenced by BAP and their interaction with Kin or NAA. MS medium containing 13.2 μM BAP and 4.6 μM Kin was most effective for shoot multiplication from tTCL nodal explants. Upon this medium, percent response for shoot proliferation was 100% with an average of 32.6 shoot buds per tTCL nodal explant. Regenerated shoots from tTCL nodal explants were rooted on the growth regulator free MS medium. The rooted plantlets were successfully acclimatized and established in soil with a survival frequency of 90-100%. Random amplified polymorphic DNA (RAPD) markers were used to evaluate the genetic fidelity of the micropropagated plants. RAPD profile analysis indicated that micropropagated plants were genetically similar to mother plant.  相似文献   

Short Communication: Stocking Density-Dependant Growth and Survival of Asian Sun Catfish,Horabagrus brachysoma (Gunther 1864) Fry During Hatchery Rearing     

S. K. Sahoo  S. S. Giri  S. Chandra  A. K. Sahu 《Journal Of Applied Aquaculture》2013,25(1):86-91

The present study was conducted to determine the optimum stocking density of Horabagrus brachysoma fry during fingerling production in the hatchery. For this purpose, four density levels (400, 800, 1200 and 1600 fry/0.6 m³) were considered. The experiment of fry rearing continued for a period of six weeks. The total length and wet weight of fry were significantly highest (P < 0.05) at 400 fry/0.6 m³ density compared to the other three density treatments during the entire rearing period. At harvesting, the length and weight of fry stocked at 1200 and 1600 numbers/0.6 m³ were similar to each other, and both were significantly lower (P < 0.05) than those of fish stocked at 800/0.6 m³. The decreased SGR, percent weight gain, and survival were also observed at the two highest density treatments. But the total biomass was observed to be highest (P < 0.05) at 1600/0.6 m³ density compared to those of the other three densities. If individual size and number of individuals for stocking are not constraints, the maximum number of surviving fry from a minimum rearing space was achieved at a stocking density of 1600 fry/0.6 m³ tank.  相似文献   

Immunomodulatory effect of recombinant RNA‐dependent RNA polymerase protein of Macrobrachium rosenbergii nodavirus in giant freshwater prawn M. rosenbergii     

Pramoda Kumar Sahoo  Mudagandur S Shekhar  Abhilipsa Das  Manickam Dilli Kumar  Bindu R Pillai  A S Sahul Hameed 《Aquaculture Research》2012,43(8):1096-1106

The present study evaluated the role of recombinant RNA‐dependent RNA polymerase (RdRp) protein of Macrobrachium rosenbergii nodavirus (MrNV) in modulating the immune response and in reducing MrNV load in infected prawn. In the first experiment, prawns (25–30 g) were injected with recombinant RdRp protein (RP) at a concentration of 0, 1.0 and 10 μg, and immune parameters and expression of some immune‐related genes were measured up to 14 days post injection (p.i.). In the second experiment, early juveniles were injected with a similar dose of RdRp and animals were challenged by immersion with MrNV. The infection status was detected in muscles by nested RT‐PCR up to 21 days post challenge. Prawn injected with higher concentration of RP showed significantly higher total haemocyte count at different period post injection. Significant up‐regulation of immune‐related genes was observed within 24 h in prawn treated with lower dose of RP and after 7 days p.i. at higher level of RP injection compared with adult control. Most of the tested samples (63%) were found to be RT‐PCR positive for MrNV at 48 h of post‐immersion challenge. After 14 days, MrNV was detected only in control prawn, while both RP‐injected groups were MrNV negative. This study elucidated the potential viral load reduction role played by RdRP in MrNV‐infected prawn.  相似文献   

Purification and characterization of a β–glucan binding protein from the haemolymph of freshwater prawn Macrobrachium rosenbergii          下载免费PDF全文

Jyotirmaya Mohanty  Pramoda Kumar Sahoo  Bindu R. Pillai  Swagatika Mohanty  Sushil Kumar Garnayak  Shailesh Kumar 《Aquaculture Research》2015,46(1):95-104

β‐glucan binding protein (βGBP), a pattern recognition protein was purified from the haemolymph of freshwater prawn Macrobrachium rosenbergii by heparin affinity chromatography that showed a single band in native gradient PAGE. The β‐glucan binding property of the purified protein was confirmed in a phenoloxidase (PO) assay, where addition of βGBP along with β‐glucan increased the specific PO activity compared with that of β‐glucan alone. The molecular weight of the βGBP was found to be ~316 kDa on gel filtration chromatography. In SDS‐PAGE, βGBP molecule was reduced to one polypeptide chain of molecular weight ~113 kDa. Thus the βGBP in M. rosenbergii is possibly a homotrimeric molecule. The purified sample run on unreduced condition in SDS‐PAGE also revealed a similar size band (~113 kDa) and hence, the polypeptide chains of βGBP are held by non‐covalent interactions. The purified βGBP samples run in native PAGE was stained positively with alcian blue for carbohydrates and Sudan black for lipids indicating the βGBP to be a glycolipoprotein. With rabbit polyclonal anti‐βGBP serum developed, an indirect ELISA was standardized and the normal βGBP concentration in adult M. rosenbergii serum was quantified to be ~2 mg mL^?1. Furthermore, the applicability of the developed ELISA is discussed.  相似文献   

Investigation of transferrin polymorphism in Garole sheep     

Devesh K. Yadav  Subhash Taraphder  Ajit K. Sahoo  K. C. Dhara 《Veterinary research communications》2010,34(3):277-284

The aim of this study was to determine the genetics of polymorph systems of Transferrin in Garole sheep breed. The present study was conducted on 95 adult Garole sheep comprising 52 ewes and 43 rams, maintained at Sheep and Goat Breeding Farm of West Bengal University of Animal and Fishery Sciences, West Bengal, during the period from April–September, 2009. The polymorphism of transferrin was determined through SDS-Polyacrylamide gel electrophoresis technique. It was found that the transferrin type was controlled by five codominant alleles (TfA, TfB, TfC TfD and TfE) in Garole sheep. These five alleles, because of co-dominant nature of inheritance, determined the occurrence of nine transferrin genotypes in the analyzed flock. Four (TfAA, TfBB, TfCC and TfDD) of these were homozygous and the remaining five (TfAD, TfBC, TfBD, TfCD and TfDE) heterozygous. It was found that the TfDD genotype (0.263) was predominant while TfDE genotype (0.042) was least common in the analyzed flock. Frequencies of other genotypes were as: TfCD(0.242), TfBD(0.126), TfCC(0.084), TfBB(0.074), TfAA(0.063), TfAD and TfBC (0.053 for each genotype ) in whole population. From the result it was found that in whole population combined, the heterozygotic genotypic frequency (0.516) was more than that of homozygotic genotypic frequency (0.484). Considerable variations were recognized in the frequencies of transferrin alleles. In the whole population frequencies of transferrin alleles were found to be TfA = 0.089, TfB = 0.163, TfC = 0.232, TfD = 0.495 and TfE = 0.021. Transferrin system has shown an absence of genetic equilibrium among the analyzed herd (χ2 value = 51.31). In conclusion, there were polymorphism in Transferrin types and the presence of differences among the frequencies of the five alleles by categories could be a source of genetic variation in Garole sheep.  相似文献