Radial variation of vessel size and distribution in cork oak wood (<Emphasis Type="Italic">Quercus suber</Emphasis> L.)     

Sofia Leal  Vicelina B. Sousa  Helena Pereira 《Wood Science and Technology》2007,41(4):339-350

Quercus suber L. is an important species producing cork whose wood characteristics have not been investigated a lot. Cork oak wood vessels are a striking feature and the most abundant wood tissue largely influencing density and permeability. Vessel size and distribution were studied in approximately 40 year-old and never debarked cork oaks by continuously measuring along the radial direction in the transverse section of wood discs taken at 1.3 m of height using image analysis techniques. The vessel size increases with age from 7660 ± 2286 to 21136 ± 6119 μm², the conductive area from 5.4 ± 2.2 to 11.6 ± 3.9%, and the vessel density remains approximately constant between 5.2 ± 1.5 and 7.3 ± 3.5 vessels/mm². In comparison with ring-porous and some evergreen oaks, cork oaks show a similar conductive area but smaller vessels. Vessel architecture is known to play an important role on oaks tolerance to hydric stress, and these cork oak trees were growing under very harsh edaphoclimatic conditions, not tolerated by other oaks. The well-developed and deep root system allowing access to constant water supply may contribute to the cork oak’s relatively high conductive area.  相似文献   

Effects of Addition of Tissue‐Type Plasminogen Activator in In Vitro Fertilization Medium on Bovine Embryo Development and Quality          下载免费PDF全文

F Krania  E Dovolou  CA Rekkas  EK Theodosiadou  I Pappas  GS Amiridis 《Reproduction in domestic animals》2015,50(1):112-120

Plasminogen activators/Plasmin system plays pivotal role in regulating reproductive functions of mammals. Here, we examined the effects of modification of in vitro fertilization medium (IVF medium) with the addition of tissue‐type plasminogen activator (t‐PA), on bovine embryo development and quality, assessed by quantification of expression of various genes related to metabolism, oxidation, implantation and apoptosis. In addition, plasminogen activator activity (PAA) and plasminogen activator inhibition (PAI) were measured in the spent media. After conventional IVM, 2016 cumulus‐oocyte complexes (COCs) were divided into four groups with modified composition of the IVF medium containing t‐PA and/or its inhibitor epsilon‐aminocaproic acid (control, t‐PA, t‐PA+ε‐ACA, ε‐ACA). Presumptive zygotes were cultured for 8 days in synthetic oviductal fluid (SOF) medium; gene expression studies were carried out on morulae and blastocysts. t‐PA alone significantly suppressed cleavage and blastocyst formation rates, but this effect was neutralized by the addition of ε‐ACA. PAA in the treated group was significantly reduced by ε‐ACA, but without total elimination. Significant differences were detected in the expression of genes related to apoptosis and/or cell cycle arrest (BAX, BCL2L1, KAT2B) between embryos produced in t‐PA‐modified media and controls, giving an overall notion that the inferior developmental competence of treated embryos may be attributed to apoptotic phenomena induced by t‐PA. In conclusion, it appears that excessive t‐PA content in the IVF media, suppresses blastocyst formation rate, possibly due to induction of apoptotic phenomena.  相似文献   

Molybdenum deprivation,purine ingestion and an astrocyte‐associated motor neurone syndrome in sheep: assumed clinical effects of inosine          下载免费PDF全文

CA Bourke 《Australian veterinary journal》2015,93(3):79-83

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Detection of living Bursaphelenchus xylophilus in wood,using reverse transcriptase loop‐mediated isothermal amplification (RT‐LAMP)          下载免费PDF全文

I. Leal  E. Allen  B. Foord  J. Anema  C. Reisle  A. Uzunovic  A. Varga  D. James 《Forest Pathology》2015,45(2):134-148

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Oral Recombinant Feline Interferon-Omega as an alternative immune modulation therapy in FIV positive cats: Clinical and laboratory evaluation     

S. Gil  R.O. Leal  D. McGahie  N. Sepúlveda  A. Duarte  M.M.R.E. Niza  L. Tavares 《Research in veterinary science》2014

Recombinant-Feline Interferon-Omega (rFeIFN-ω) is an immune-modulator licensed for use subcutaneously in Feline Immunodeficiency virus (FIV) therapy. Despite oral protocols have been suggested, little is known about such use in FIV-infected cats. This study aimed to evaluate the clinical improvement, laboratory findings, concurrent viral excretion and acute phase proteins (APPs) in naturally FIV-infected cats under oral rFeIFN-ω therapy (0.1 MU/cat rFeIFN-ω PO, SID, 90 days). 11 FIV-positive cats were treated with oral rFeIFN-ω (PO Group). Results were compared to previous data from 7 FIV-positive cats treated with the subcutaneous licensed protocol (SC Group). Initial clinical scores were similar in both groups. Independently of the protocol, rFeIFN-ω induced a significant clinical improvement of treated cats. Concurrent viral excretion and APP’s variation were not significant in the PO Group. Oral rFeIFN-ω can be an effective alternative therapy for FIV-infected cats, being also an option for treatment follow-up in cats submitted to the licensed protocol.  相似文献   

Identification and quantification of benzimidazole resistance polymorphisms in Haemonchus contortus isolated in Northeastern Brazil     

Jessica Maria Leite dos Santos  Jomar Patrício Monteiro  Wesley Lyeverton Correia Ribeiro  Iara Tersia Freitas Macedo  Ana Lourdes Fernandes Camurça-Vasconcelos  Luiz da Silva Vieira  Claudia Maria Leal Bevilaqua 《Veterinary parasitology》2014,199(3-4):160-164

Haemonchus contortus is the most prevalent nematode in Brazil. The objective of this study was to select 6 populations of H. contortus of known or suspected benzimidazole resistance status and characterize these using quantitative real-time polymerase chain reaction (qPCR) for single nucleotide polymorphisms (SNPs) F200Y, F167Y and E198A in the β-tubulin isotype 1 gene. qPCR was performed using DNA from a pool of 10 adult male H. contortus from a single animal per farm. Faecal egg count reduction test (FECRT) and egg hatch test (EHT) were used to determine the resistance status. Samples were obtained from 6 farms located in 5 counties in the Ceará State: Tauá, Boa Viagem, Quixadá, Santa Quitéria and Solonópole. The inbred-susceptible-Edinburgh (ISE) isolate was used as reference for comparative purposes in the qPCR. Benzimidazole resistance was detected by FECRT on all farms with efficacy values ranging from 0 to 51%. EC50 values as determined by EHT were all above 1.49 μg/ml. High frequencies of the resistant SNPs F200Y and F167Y alleles were detected but no resistance was detected at SNP E198A. Our results suggest that the SNPs F167Y and F200Y are both important for benzimidazole resistance in the studied populations.  相似文献   

The Influence of Morphology,Follicle Size and Bcl‐2 and Bax Transcripts on the Developmental Competence of Bovine Oocytes     

THC De Bem  PR Adona  FF Bressan  LG Mesquita  MR Chiaratti  FV Meirelles  CLV Leal 《Reproduction in domestic animals》2014,49(4):576-583

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Streptococcus dysgalactiae as an agent of septicaemia in Nile tilapia, Oreochromis niloticus (L.)     

Netto LN  Leal CA  Figueiredo HC 《Journal of fish diseases》2011,34(3):251-254

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Cerebellar degeneration in goats grazing Solanum cinereum (Narrawa burr)     

CA BOURKE 《Australian veterinary journal》1997,75(5):363-365

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Effect of Drechslera (Helminthosporium) sacchari Toxin upon the Permeability of Sugarcane Leaf and Callus Tissues     

M. Ramos  Leal R. H. Maribona 《Plant Breeding》1991,107(3):242-247

Sugarcane leaves and calli from highly susceptible and resistant varieties to eyespot disease were used to evaluate the Drechslera sacchari toxin effect at different concentrations and incubation times by measuring electrolyte leakage. This expression of disease resistance was observed not only in the leaf but also in the callus. Furthermore, the growth of resistant calli MS medium supplemented with DS toxin, was higher when compared to the susceptible ones. The possibility of obtaining resistant somaclones is confirmed.  相似文献