A fixation with fixation     

Leigh GJ 《Science (New York, N.Y.)》1995,268(5212):827-828

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Long term effects of saline irrigation on the yield and growth of mature Williams pear trees     

Myers  Bronwyn A.  West  Dennis W.  Callinan  Leigh  Hunter  Colin C. 《Irrigation Science》1995,16(1):35-46

Salt tolerance of mature Williams Bon Cretien pear trees was assessed in a field trial on a duplex, slowly permeable clay loam. The trees were irrigated with a range of salinities; electrical conductivity of irrigation water (EC_w) of 0.2 to 1.4 dS/m by flood for seven years or 0.2 to 2.1 dS/m by microjet sprinklers for nine years. Water-table levels were maintained below 3 m by a groundwater pump. Yield and leaf ion content were assessed during the treatment period. Aspects of growth and physiology were monitored in the 0.2 and 2.1 dS/m microjet treatments during the seventh irrigation season.Soil profile salinities varied between 3.0 and 4.3 dS/m for the most saline flood treatment and from 1.5 to 2.6 dS/m for the most saline microjet treatment. Soil sodicity (sodium absorption ratio) increased during the experiment, reaching a maximum of 9 in the most saline treatments. The salinity treatments caused reduced yields after seven years. In the most saline treatment (EC_w = 2.1 dS/m, microjet-irrigated), yield decreased to about 60 and 50% of the control in the eighth and ninth years, respectively, and 40% of trees were dead in the ninth year. Leaf ion concentrations (in January) of the most saline treatment were at excess levels (>0.1% Cl and >0.02% Na) from 1982 to 1990. There were significant (P<0.01) negative linear relationships between yield in 1990 and leaf Na and Cl, measured both in 1990 and in 1989. During the seventh season of saline irrigation, lateral shoot growth was reduced, leaves and fruit were smaller and leaf fall was earlier in the 2.1 dS/m treatment compared with the control. Dawn and midday water potential and osmotic potential were not significantly affected by saline irrigation. Midday CO₂-assimilation rates (A) and leaf conductance to water vapour diffusion (g) were similar for 2.1 dS/m irrigated and control trees, however there was a trend towards a reduction in A and g of these salt-treated trees late in the irrigation season when leaf Na and Cl had increased to 250 and 240 mM (tissue water basis) respectively.  相似文献   

Survival of various ERIC-genotypes of Shiga toxin-producing Escherichia coli in well water     

Leigh Watterworth  Bruce Rosa  Heidi Schraft  Edward Topp  Kam Tin Leung 《Water, air, and soil pollution》2006,177(1-4):367-382

Recently, there has been a surge of interest in understanding the survival of Shiga toxin-producing Escherichia coli (STEC) in aquatic environments. Fifteen strains of STEC were monitored, individually, in untreated well water samples incubated at 10 and 22^{^}C for 56 days. The strains were selected from three serogroups (O26, Ol11 and O157) and represented five distinct ERIC (enterobacterial repetitive intergenic concensus)-genotypes. The microcosms were prepared in triplicate and inoculated at an initial cell density of about 7.0 log CFU/ml well water. At 10^{^}C, the cell density of five STEC strains fell below the detection limit of 0.8 log CFU/ml by day 56. Of the ten persisting strains, four showed superior survival with cell densities decreasing to an average of about 5 log CFU/ml while the remaining six strains showed moderate levels of survival, decreasing to an average cell density of about 3 log CFU/ml. At 22^{^}C, strain H32 (genotype I) and H15 (genotype B) persisted at 1.1 log CFU/ml and 2.2 log CFU/ml in 56 days, respectively. The other 13 STEC strains dropped below the detection limit between weeks 3 to 8. The 15 strains demonstrated highly variable levels of survival with no correlation between ERIC-genotypes or serogroups and the strains' ability to persist in the well water samples. Although strain H32 (O157:H7) persisted significantly longer than strain H22 (O157:H7) in natural well water at both 10 and 22^{^}C, both strains survived equally well in sterile well water, indicating that individual STEC strains vary in their ability to compete with background microbial populations.  相似文献   

A Comparison of the Effects of Unilateral Thyroarytenoid Lateralization Versus Cricoarytenoid Laryngoplasty on the Area of the Rima Glottidis and Clinical Outcome in Dogs with Laryngeal Paralysis     

Leigh G. Griffiths  BA  VetMB  DSAS  MRCVS    Martin Sullivan  BVMS  PhD  DVR  Diplomate ECVDI  MRCVS    Stuart W. J. Reid  BVMS  PhD  MRCVS 《Veterinary surgery : VS》2001,30(4):359-365

OBJECTIVE: To compare the clinical effects of unilateral thyroarytenoid lateralization versus unilateral cricoarytenoid laryngoplasty for the treatment of canine laryngeal paralysis. Study Design-A prospective, clinical trial. ANIMALS: Twenty client-owned dogs admitted to Glasgow University Veterinary Hospital (GUVH) between 1997 and 1999 with a diagnosis of laryngeal paralysis. METHODS: A thorough evaluation of each dog was undertaken that included history, physical and neurologic examinations, complete blood count, serum biochemistry profile, serum thyroxine (T4) level, and thyroid-stimulating hormone (TSH) assay. The exercise tolerance of each dog was investigated by assessing respiratory rate and arterial blood gas analysis at rest and after 5 minutes of mild exercise. Animals were included in the study on the basis of a definitive diagnosis of laryngeal paralysis made by endoscopic observation of the larynx in the lightly anesthetized patient. The animals were randomly assigned to 1 of 2 treatment groups: unilateral thyroarytenoid lateralization or unilateral cricoarytenoid laryngoplasty, with all procedures being performed by the same surgeon. Video images of the rima glottidis were recorded pre- and postoperatively using video endoscopy. Video images were digitized and the area of the rima glottidis measured using image-analysis software. An increase in the area of the rima glottidis was expressed as a percentage of the preoperative area. All animals were reassessed 6 weeks' postoperatively, and exercise tolerance tests were repeated. RESULTS: The mean increase in the area of the rima glottidis postoperatively when compared with the preoperative area was 140% for those animals undergoing unilateral thyroarytenoid lateralization (range, 120%-158%) and 207% for those animals undergoing unilateral cricoarytenoid laryngoplasty (range, 183%-228%). This difference was statistically significant (P < .0001). At the 6-week postoperative examination, there was no significant difference in improvement between the 2 groups when compared on either a clinical basis or exercise tolerance tests. The mean surgical time in the 2 groups was 25 minutes for thyroarytenoid lateralization and 43 minutes for cricoarytenoid laryngoplasty. This was a statistically significant difference (P = .002). CONCLUSION: Although increase in area of the rima glottidis was significantly greater after unilateral cricoarytenoid laryngoplasty compared with unilateral thyroarytenoid lateralization, no difference in clinical outcome could be detected. However, the cricoarytenoid laryngoplasty procedure required significantly more surgical time. CLINICAL RELEVANCE: Both unilateral cricoarytenoid laryngoplasty and unilateral thyroarytenoid lateralization are successful methods for the treatment of laryngeal paralysis; however, thyroarytenoid lateralization requires less surgical time to perform.  相似文献   

Evaluation of the anaesthetic effects of combinations of ketamine, medetomidine, romifidine and butorphanol in European badgers (Meles meles)     

Davison KE  Hughes JM  Gormley E  Lesellier S  Costello E  Corner LA 《Veterinary anaesthesia and analgesia》2007,34(6):394-402

OBJECTIVE: To evaluate the effects of three anaesthetic combinations in adult European badgers (Meles meles). STUDY DESIGN: Prospective, randomized, blinded, experimental trial. ANIMALS: Sixteen captive adult badgers. METHODS: The badgers were each anaesthetized by intramuscular injection using the three techniques assigned in random order: romifidine 0.18 mg kg(-1), ketamine 10 mg kg(-1) and butorphanol 0.1 mg kg(-1) (RKB); medetomidine 0.1 mg kg(-1), ketamine 9 mg kg(-1) and butorphanol 0.1 mg kg(-1) (MKB); and medetomidine 0.1 mg kg(-1) and ketamine 10 mg kg(-1) (MK). Initial drug doses were calculated based on a body mass of 10 kg. Additional anaesthetic requirements, time to drug effect, duration of action and recovery from anaesthesia were recorded. Heart rate and rhythm, respiratory rate and rhythm, rectal and subcutaneous microchip temperature and oxygen saturation were recorded every 5 minutes. Depth of anaesthesia was assessed using: muscle tone; palpebral and pedal reflexes; and tongue relaxation at these time points. Blood samples and a tracheal aspirate were obtained under anaesthesia. Atipamezole was administered if the badger had not recovered within 60 minutes Parametric data were analysed using anova for repeated measures, and nonparametric data using Friedman's, and Cochran's Q tests: p < 0.05 was considered significant. RESULTS: All combinations produced good or excellent muscle relaxation throughout the anaesthetic period. RKB had the shortest duration of anaesthesia (16.8 minutes compared with MKB 25.9 minutes and MK 25.5 minutes) and antagonism was not required. RKB depressed respiratory rate less than MK and MKB. There was no significant difference between techniques for heart rate and rhythm. CONCLUSIONS AND CLINICAL RELEVANCE: All combinations provided anaesthetic conditions suitable for sampling and identification procedures in adult badgers. The RKB protocol provided a significantly shorter period of anaesthesia when compared with the combinations containing medetomidine.  相似文献   

Effects of increased dosages of the Mycoplasma gallisepticum vaccine MYCOVAC-L in layer chickens subsequently challenged with virulent M. gallisepticum: egg production and serologic response     

Evans JD  Leigh SA  Branton SL  Collier SD 《Avian diseases》2007,51(4):912-917

Ten-week-old Hy-Line Commercial W-36 pullets were spray-vaccinated with MYCOVAC-L at the manufacturer's recommended dosage (1x) or at 15 times that rate (15x). At 22 or 45 wk of age, subsets of 1x- and 15x-vaccinated pullets were challenged with the virulent Mycoplasma gallisepticum (MG) strain Rlow. Percent hen-day egg production was determined through week 55. Analyses for treatment effects on overall (22-56 wk) percent hen-day egg production revealed no significant differences between nonchallenged 1x and nonchallenged 15x MYCOVAC-L treatments. Among 1x MYCOVAC-L-vaccinated groups, Rlow challenge at 45 wk corresponded to significantly (P < or = 0.01) lower overall egg production compared with the unchallenged 1x-vaccinated control (70.88% vs. 79.15%, respectively). Conversely, at the 15x MYCOVAC-L dosage level, overall egg production was not significantly affected by virulent MG challenge at 45 wk compared with its unchallenged counterpart (84.09% vs. 81.03%, respectively) and could indicate increased protection from virulent MG challenge. Serologic monitoring indicated the virulent MG challenge was consistently (100%) associated with seroconversion. Comparisons among the nonchallenged experimental treatments indicated that vaccinations at the 15x MYCOVAC-L dosage rate were associated with a greater seroconversion rate at weeks 21, 27, and 44, but not at week 50.  相似文献   

Early, microbially driven follicular reactions in the neonatal piglet do not contribute to expansion of the immunoglobulin heavy chain V-D-J repertoire     

Wilson S  Norton P  Leigh J  Bailey M 《Veterinary immunology and immunopathology》2007,118(1-2):105-112

Selective microbial colonisation of germ-free piglets is reported to result in expansion of immunoglobulin V(H)- and D(H)-segment usage from an initially limited repertoire. Here, the response of the palatine tonsil to microbial colonisation was compared in age-matched conventionally reared and germ-free piglets. At 3 and 5 days after birth an expansion in the B-cell follicle area was observed in the conventional, microbially colonised animals, which was not seen in the germ-free piglets. Consistent with this observation, sequencing of re-arranged heavy chain V-D-J units demonstrated accumulation of point mutations indicating somatic hypermutation in the conventional, microbially colonised piglets but not in the germ-free animals. However, V(H)- and D(H)-segment usage and CDR3 length did not differ between the groups. The results suggest that the follicle reaction observed occurs in response to microbial challenge, involves proliferation and somatic hypermutation of B-cells but does not expand repertoire or generate classical, isotype-switched memory B-cells. We suggest that microbial colonisation of neonatal piglets drives immunological competence in two stages: first, an antigen non-specific, follicular reaction which expands immunological compartments; and second, microbe driven changes in V-segment usage which expand immunological repertoire.  相似文献   

Extending Our Reach: Surveying,Analyzing, and Planning Outreach to Extension Staff     

Kristen Leigh Mastel 《Journal Of Agricultural & Food Information》2014,15(4):268-281

This study describes how University of Minnesota Extension staff locate and use information to accomplish daily activities. The major findings were: (a) information seeking is a daily or weekly need; (b) staff use predominately online resources from a variety of sources—peer-reviewed journals, technical or research reports, and government information—and use search engines most frequently to locate them; and (c) Extension staff are aware of the majority of library resources and services. Extension staff welcome advanced information-seeking tools and techniques for professional development.  相似文献   

AIDS. Promote HIV chemoprophylaxis research, don't prevent it     

Grant RM  Buchbinder S  Cates W  Clarke E  Coates T  Cohen MS  Delaney M  Flores G  Goicochea P  Gonsalves G  Harrington M  Lama JR  MacQueen KM  Moore JP  Peterson L  Sanchez J  Thompson M  Wainberg MA 《Science (New York, N.Y.)》2005,309(5744):2170-2171

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A reversible synthetic rotary molecular motor     

Hernández JV  Kay ER  Leigh DA 《Science (New York, N.Y.)》2004,306(5701):1532-1537

The circumrotation of a submolecular fragment in either direction in a synthetic molecular structure is described. The movement of a small ring around a larger one occurs through positional displacements arising from biased Brownian motion that are kinetically captured and then directionally released. The sense of rotation is governed solely by the order in which a series of orthogonal chemical transformations is performed. The minimalist nature of the [2]catenane flashing ratchet design permits certain mechanistic comparisons with the Smoluchowski-Feynman ratchet and pawl. Even when no work has to be done against an opposing force and no net energy is used to power the motion, a finite conversion of energy is intrinsically required for the molecular motor to undergo directional rotation. Nondirectional rotation has no such requirement.  相似文献