Endocrine Regulation of the Establishment of Spermatogenesis in Pigs     

KC Caires  JA Schmidt  AP Oliver  J de Avila  DJ McLean 《Reproduction in domestic animals》2008,43(S2):280-287

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Identification of RSVP14 and RSVP20 components by two-dimensional electrophoresis and Western-blotting.   总被引：1，自引：0，他引：1  

JA Cardozo  M Fernández‐Juan  JA Cebrián‐Pérez  T Muiño‐Blanco 《Reproduction in domestic animals》2008,43(1):15-21

We have already shown that RSVP14 and RSVP20, two ram seminal plasma (SP) proteins postulated to be involved in sperm capacitation and gamete interaction can protect spermatozoa against cold-shock. In this study, we use two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) for the analysis of SP proteins of Rasa Aragonesa rams, using enhanced protein solubilization in the presence of tributyl phosphine (TBP) and a polyacrylamide linear gradient gel with a narrow pH range (4-7). The image analysis of the 2D map detected 195 protein spots, with isoelectric points (pIs) ranging from 4.5 to 6.6, and molecular weight (M(r)) from 11.7 to 90.4. Staining of 2D gels with Pro-Q Emerald 300 Glycoprotein Stain revealed that most significant proteins in ram SP are glycosylated. The removing of protein N-linked oligosaccharides improved the gel resolution. 2D-PAGE analysis of the whole fraction 6 (F6) separated from ram SP by exclusion chromatography showed six main protein spots, four (a, b, c, d) in the 14 kDa and two (e, f) in the 20 kDa region. Western-blot analyses indicated that the anti-P14 antibody recognized four spots on the SP map, 4, 5, 6 and 7, that matched with spots a, b, c, d of F6 map. The anti-P20 antibody recognized spots 13 and 14 of SP map that corresponded to spots e, f of F6 map. The deduced sequences by de novo sequencing evidenced that protein spots 7 and 13 have significant similarities to BSP family, while protein spots 4 and 14 did not appear to be homologous with any reported protein in the current mammalian Proteinbank databases.  相似文献   

Effect of Chemical or Electrical Activation of Bovine Oocytes on Blastocyst Development and Quality   总被引：2，自引：0，他引：2  

MP Milazzotto  WB Feitosa  ARS Coutinho  MD Goissis  VP Oliveira  MEOA Assumpção  JA Visintin 《Reproduction in domestic animals》2008,43(3):319-322

Activation of in vitro‐matured (IVM) oocytes is essential for successful embryo production following nuclear transfer (NT) or intracytoplasmic sperm injection (ICSI). This study was designed to compare the rates of blastocyst production and embryo quality (as measured by numbers of viable cells) following parthenogenetic activation with electrical pulse or the use of two different calcium ionophores, A23187 (CA) or ionomycin (IO), with or without the addition of bovine serum albumin (BSA). IVM oocytes with a first polar body were randomly allocated to five treatment groups: CA (5 μm CA, 5 min; n = 88), CA + BSA (5 μm CA, 5 min; BSA, 5 min; n = 90), IO (5 μm IO, 5 min; n = 91), IO + BSA (5 μm IO, 5 min; BSA, 5 min; n = 86) and EL (two pulses of 1.5 kV/cm, 20 μs; n = 120). Blastocyst rates were higher (p < 0.05) for CA (54.4%), IO (51.4%) and EL (54.5%) than for IO + BSA (18.3%). Treatment CA + BSA (39.8%) did not differ from the others. There was no difference (p > 0.05) among treatments in total number of cells. However, the percentage of viable cells was reduced in CA (49.9%), CA + BSA (45.8%), IO (64.9%), IO + BSA (50.9%) compared with EL (82.7%). In summary, the addition of BSA to the IO treatment had an adverse effect on blastocyst production rates. Although there was no difference between electrical stimulation and chemical activation on blastocyst production rates, electrical activation resulted in blastocysts with a higher percentage of viable cells.  相似文献   

A pilot study of Australian pet owners who engage in social media and their use,experience and views of online pet health information     

LR Kogan  SJ Hazel  JA Oxley 《Australian veterinary journal》2019,97(11):433-439

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Elevating glucose and insulin secretion by carbohydrate formulation diets in late lactation to improve post‐weaning fertility in primiparous sows          下载免费PDF全文

TY Chen  D Lines  C Dickson  C Go  RN Kirkwood  P Langendijk 《Reproduction in domestic animals》2016,51(5):813-818

Primiparous (P1) sows commonly lose excessive body reserves to meet energy requirements for maintenance and milk production during lactation, and consequently, post‐weaning reproductive performance may be compromised. The present studies determined whether ad libitum feeding a glucogenic carbohydrate diet (CHO) during late lactation could stimulate insulin and glucose secretion (experiment 1) and improve subsequent litter size (experiment 2). For experiment 1, 15 P1 sows, and for experiment 2, 99 P1 sows (198.5 ± 2.7 kg) were allocated randomly according to suckled litter size (≥10 piglets), either to a CHO diet (14.3 MJ DE/kg, 19.8% crude protein) or a standard lactation diet (control; 14.2 DE MJ/kg, 19.5% crude protein) at 8 days before weaning. The CHO diet aimed to provide glucogenic content (extruded wheat, dextrose and sugar) as energy sources instead of fat sources without changing total dietary energy. Pre‐prandial plasma glucose and insulin concentrations were not influenced by treatments. However, post‐prandial plasma glucose and insulin concentrations and their peaks were both higher (p < .05) compared to the control treatment. Body weight loss during lactation was relatively low at 3%–4% for both treatments and did not differ between control and CHO treatments (?7.6 ± 1.6 vs ?5.4 ± 1.2 kg; p > .05). Second litter size was not influenced by diet (p > .05), but the weaning‐to‐mating interval was shorter in CHO sows (p < .05). This study demonstrates that providing an enriched CHO diet in late lactation did influence post‐weaning follicle growth but did not improve subsequent litter size. This may be due to the primiparous sows in this study not experiencing severe negative energy balance and there was no second litter syndrome in this farm which limited the ability of diet to improve sow fertility.  相似文献   

A monolithic photovoltaic-photoelectrochemical device for hydrogen production via water splitting     

O Khaselev  JA Turner 《Science (New York, N.Y.)》1998,280(5362):425-427

Direct water electrolysis was achieved with a novel, integrated, monolithic photoelectrochemical-photovoltaic design. This photoelectrochemical cell, which is voltage biased with an integrated photovoltaic device, splits water directly upon illumination; light is the only energy input. The hydrogen production efficiency of this system, based on the short-circuit current and the lower heating value of hydrogen, is 12.4 percent.  相似文献   

Climate and vegetation history of the midcontinent from 75 to 25 ka: A speleothem record from crevice cave, missouri, USA   总被引：3，自引：0，他引：3  

JA Dorale  RL Edwards  E Ito  LA Gonzalez 《Science (New York, N.Y.)》1998,282(5395):1871-1874

Four Missouri stalagmites yield consistent overlapping records of oxygen and carbon isotopic changes and provide a climate and vegetation history with submillennial resolution from 75 to 25 thousand years ago (ka). The thorium-230-dated records reveal that between 75 and 55 ka, the midcontinental climate oscillated on millennial time scales between cold and warm, and vegetation alternated among forest, savanna, and prairie. Temperatures were highest and prairie vegetation peaked between 59 and 55 ka. Climate cooled and forest replaced grassland at 55 ka, when global ice sheets began to build during the early part of Marine Oxygen Isotope Stage 3.  相似文献   

A long-snouted predatory dinosaur from africa and the evolution of spinosaurids     

PC Sereno  AL Beck  DB Dutheil  B Gado  HCE Larsson  GH Lyon  JD Marcot  OWM Rauhut  RW Sadleir  CA Sidor  DD Varricchio  GP Wilson  JA Wilson 《Science (New York, N.Y.)》1998,282(5392):1298-1302

Fossils discovered in Lower Cretaceous (Aptian) rocks in the Tenere Desert of central Niger provide new information about spinosaurids, a peculiar group of piscivorous theropod dinosaurs. The remains, which represent a new genus and species, reveal the extreme elongation and transverse compression of the spinosaurid snout. The postcranial bones include blade-shaped vertebral spines that form a low sail over the hips. Phylogenetic analysis suggests that the enlarged thumb claw and robust forelimb evolved during the Jurassic, before the elongated snout and other fish-eating adaptations in the skull. The close phylogenetic relationship between the new African spinosaurid and Baryonyx from Europe provides evidence of dispersal across the Tethys seaway during the Early Cretaceous.  相似文献   

Review of opportunities for low stress and selective control of fish     

J. A. Lines  A. R. Frost 《Aquacultural Engineering》1999,20(4):215-230

Routine inspection and selective control of livestock is an integral part of animal agriculture benefiting both animal welfare and profitability. Appropriate developments in this field may therefore also be expected within fin-fish farming. Equipment enabling some subsurface inspection of fish stocks has recently appeared on the market but this is as yet unmatched by equipment which would enable subsurface selective interaction for the purpose of sampling, further inspection or selective application of remedial measures. Currently available non-selective fish control techniques are reviewed and some potential methods for selective control are considered. Since most forms of selective control must rely on fish’s response to signals the paper is organised on the basis of the sensory systems that might be used. A brief introduction to each system is given followed by a review of any current control techniques and possibilities for developing selective control systems.  相似文献   

Priming of cowpea volatile emissions with defense inducers enhances the plant's attractiveness to parasitoids when attacked by caterpillars          下载免费PDF全文

Toby JA Bruce  Ted CJ Turlings 《Pest management science》2018,74(4):966-977

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