全文获取类型
| 收费全文 | 38229篇 |
| 免费 | 1233篇 |
| 国内免费 | 2628篇 |
专业分类
| 林业 | 6236篇 |
| 农学 | 5011篇 |
| 基础科学 | 2461篇 |
| 6778篇 | |
| 综合类 | 6941篇 |
| 农作物 | 3189篇 |
| 水产渔业 | 2400篇 |
| 畜牧兽医 | 4018篇 |
| 园艺 | 1733篇 |
| 植物保护 | 3323篇 |
出版年
| 2024年 | 44篇 |
| 2023年 | 242篇 |
| 2022年 | 647篇 |
| 2021年 | 906篇 |
| 2020年 | 825篇 |
| 2019年 | 817篇 |
| 2018年 | 3249篇 |
| 2017年 | 3445篇 |
| 2016年 | 1930篇 |
| 2015年 | 1140篇 |
| 2014年 | 1006篇 |
| 2013年 | 1237篇 |
| 2012年 | 2158篇 |
| 2011年 | 3647篇 |
| 2010年 | 3545篇 |
| 2009年 | 2578篇 |
| 2008年 | 2570篇 |
| 2007年 | 2798篇 |
| 2006年 | 1206篇 |
| 2005年 | 1154篇 |
| 2004年 | 638篇 |
| 2003年 | 623篇 |
| 2002年 | 472篇 |
| 2001年 | 488篇 |
| 2000年 | 523篇 |
| 1999年 | 563篇 |
| 1998年 | 559篇 |
| 1997年 | 443篇 |
| 1996年 | 432篇 |
| 1995年 | 382篇 |
| 1994年 | 317篇 |
| 1993年 | 300篇 |
| 1992年 | 276篇 |
| 1991年 | 260篇 |
| 1990年 | 190篇 |
| 1989年 | 136篇 |
| 1988年 | 125篇 |
| 1987年 | 76篇 |
| 1986年 | 30篇 |
| 1985年 | 22篇 |
| 1984年 | 18篇 |
| 1983年 | 15篇 |
| 1982年 | 17篇 |
| 1981年 | 16篇 |
| 1980年 | 6篇 |
| 1979年 | 4篇 |
| 1977年 | 4篇 |
| 1968年 | 4篇 |
| 1963年 | 2篇 |
| 1956年 | 1篇 |
排序方式： 共有10000条查询结果，搜索用时 281 毫秒
11.
12.
Recep Karakaş 《Paddy and Water Environment》2017,15(4):919-930
The birds of Kabakl? Pond and its near surrounding were surveyed between October 2014 and December 2015 periods. During the study period, 105 bird species that belong to 12 orders and 34 families were detected and represented. Among these species determined, 17 of them certainly, 19 probably and 11 possibly breed in the area, while 58 of them are wintering or transitory migrating birds for the area. Among the recorded species, seven are globally threatened, while 20 are threatened for Turkey without least concern species. Obtained information suggests that this small artificial area has a great importance for many bird species related to habitat that have. Habitat preferences of species were analysed, and it was determined that the most preferred habitats are pond (water surface), wooded and agricultural zones, respectively. Also, the maximum bird number obtained during April and December, while maximum bird species richness observed during April. Results suggest that artificial ponds can be important for birds as feeding, resting and breeding area due to rapidly decline in natural wetland areas. Effective protection of the area requires the prevention of illegal hunting, fishing, human disturbance and pasturing. Also, in order to extend the existing reed and meadow areas, convenient plantation of natural plants at around of pond has a critic role for waterfowl. 相似文献
13.
Mariangela Marrelli Stefania La Grotteria Fabrizio Araniti Filomena Conforti 《Plant foods for human nutrition (Dordrecht, Netherlands)》2017,72(3):274-279
There is a great interest in the nutritional value of vegetables and fruits and how the habitat affects nutritive and biological properties. In vitro studies here reported were performed to evaluate the inhibitory activity of formulations from edible plant on pancreatic lipase. The aim of this study was also to evaluate the biovariability of L. comosa (L.) Parl. bulbs from Italy. The wild bulbs were compared with the same cultivated species that are commonly commercialized to identify samples with the best quality for a potential therapeutic application. Hydroalcoholic extract and polar fraction of wild bulbs showed a very important pancreatic lipase inhibitory activity, with IC50 values of 0.166 ± 0.005 and 0.153 ± 0.005 mg/mL, respectively. In order to characterize the extracts, gas chromatography associated with mass spectrometry (GC/MS) analysis was performed, revealing the predominance of palmitic acid. Phenolic and flavonoid composition was also evaluated. L. comosa extract obtained from wild bulbs demonstrated both antioxidant and anti-obesity activities that might be attributed to a wide range of present phenolic compounds. 相似文献
14.
M. Akhlasur Rahman Isaac Kofi Bimpong J. B. Bizimana Evangeline D. Pascual Marydee Arceta B. P. Mallikarjuna Swamy Faty Diaw M. Sazzadur Rahman R. K. Singh 《Rice》2017,10(1):47
Background
Salinity is one of the most severe and widespread abiotic stresses that affect rice production. The identification of major-effect quantitative trait loci (QTLs) for traits related to salinity tolerance and understanding of QTL × environment interactions (QEIs) can help in more precise and faster development of salinity-tolerant rice varieties through marker-assisted breeding. Recombinant inbred lines (RILs) derived from IR29/Hasawi (a novel source of salinity) were screened for salinity tolerance in the IRRI phytotron in the Philippines (E1) and in two other diverse environments in Senegal (E2) and Tanzania (E3). QTLs were mapped for traits related to salinity tolerance at the seedling stage.Results
The RILs were genotyped using 194 polymorphic SNPs (single nucleotide polymorphisms). After removing segregation distortion markers (SDM), a total of 145 and 135 SNPs were used to construct a genetic linkage map with a length of 1655 and 1662 cM, with an average marker density of 11.4 cM in E1 and 12.3 cM in E2 and E3, respectively. A total of 34 QTLs were identified on 10 chromosomes for five traits using ICIM-ADD and segregation distortion locus (SDL) mapping (IM-ADD) under salinity stress across environments. Eight major genomic regions on chromosome 1 between 170 and 175 cM (qSES1.3, qSES1.4, qSL1.2, qSL1.3, qRL1.1, qRL1.2, qFWsht1.2, qDWsht1.2), chromosome 4 at 32 cM (qSES4.1, qFWsht4.2, qDWsht4.2), chromosome 6 at 115 cM (qFWsht6.1, qDWsht6.1), chromosome 8 at 105 cM (qFWsht8.1, qDWsht8.1), and chromosome 12 at 78 cM (qFWsht12.1, qDWsht12.1) have co-localized QTLs for the multiple traits that might be governing seedling stage salinity tolerance through multiple traits in different phenotyping environments, thus suggesting these as hot spots for tolerance of salinity. Forty-nine and 30 significant pair-wise epistatic interactions were detected between QTL-linked and QTL-unlinked regions using single-environment and multi-environment analyses.Conclusions
The identification of genomic regions for salinity tolerance in the RILs showed that Hasawi possesses alleles that are novel for salinity tolerance. The common regions for the multiple QTLs across environments as co-localized regions on chromosomes 1, 4, 6, 8, and 12 could be due to linkage or pleiotropic effect, which might be helpful for multiple QTL introgression for marker-assisted breeding programs to improve the salinity tolerance of adaptive and popular but otherwise salinity-sensitive rice varieties.15.
In order to prevent surgical complications due to microbial infections, we have developed polypropylene suture grafted with silver nanoparticles (PPsuture/Ag nanocomposite) by a simple immersion procedure. Physical and mechanical properties of developed suture are investigated. Suture surface characteristics are examined by scanning electron microscopy (SEM) imaging and atomic force microscopy (AFM). Silver content on suture surface was determined by Inductively coupled plasma atomic emission spectroscopy (ICP-AES). The mechanical properties of developed antibacterial PP suture/Ag were studied. We note that proposed silver coating method has not affected mechanical performances of suture. Antimicrobial performances of PP suture/Ag nanocomposites against S. aureus and E. coli colonies were also investigated. 相似文献
16.
Landscape Ecology - The increasing availability of lidar data and structure from motion processing techniques is moving pattern metric research toward the development of three-dimensional (3D)... 相似文献
17.
通过对白屈菜低温应答过程的转录组分析发现膜脂不饱和化相关基因的表达在一定过程中发生变化,脂肪酸去饱和酶基因FAD2在随温度的变化趋势为正"V"型,且表达量变化显著。利用NCBI等在线软件对序列进行相关生物学信息分析,并对白屈菜FAD家族成员FAD2基因的完整开放阅读框(ORF)进行克隆,并命名为CmFAD2。选用克隆载体pMD-19-T,转化大肠杆菌DH5α,测序验证序列正确性及完整性。将目的基因与植物表达载体pRI-201-AN连接构建重组DNA pRI-201-AN-Cm FAD2,电击法转化农杆菌LBA4404,利用菌液PCR法验证成功。该基因可作为药用植物抗寒品种创制的候选基因。 相似文献
18.
【目的】探究在不同成熟度和降温贮藏方式下,LAC表达模式与鸭梨果心褐变的关系,为进一步解析鸭梨果心褐变机制提供理论依据。【方法】以鸭梨作为材料,通过对不同成熟度（早采、中采和晚采）的鸭梨进行急速降温（急降）和缓慢降温（缓降）处理,观察贮藏期间鸭梨果心褐变情况,测定漆酶（Laccase,LAC）活性及其基因LAC的相对表达量,研究LAC在鸭梨果心褐变过程中的参与作用。【结果】冷藏60 d时,晚采鸭梨出现褐变,晚采缓降处理的鸭梨果心褐变指数为0.32,是同期急速降温处理的2.56倍;在贮藏90 d时,中采缓降处理的褐变指数是0.24,中采急降处理的褐变指数仅为0.01。各个处理组在贮藏期间LAC活性多数表现为先逐渐升高后下降的趋势,晚采的果实在贮藏60 d时出现活性高峰,褐变发生;早采和中采鸭梨LAC活性高峰均在90 d时出现,褐变程度低于晚采鸭梨。在贮藏期间,缓慢降温处理的LAC活性高于急速降温处理。鸭梨LAC14和LAC7表达量呈先上升后下降的趋势,LAC6表现为先下降后上升再降低的变化趋势;中采、晚采鸭梨在贮藏60 d时,LAC14和LAC7表达量最高。【结论】与缓慢降温相比,急速降温处理减少了鸭梨果心褐变的发生。在整个贮藏期间,LAC活性呈先上升后下降然后又上升的趋势,其峰值时的活性高低次序为：晚采>中采>早采,这与果心褐变趋势一致;LAC在鸭梨果心褐变过程中上调表达。相比缓慢降温处理,急速降温处理具有较低的LAC7、LAC14和LAC6表达量。急速降温结合适时采收能够抑制LAC的上调表达,减少鸭梨果心褐变发生。 相似文献
19.
20.