A peptide of foot-and-mouth disease virus serotype Asia1 generating a neutralizing antibody response, and an immunostimulatory peptide   总被引：2，自引：0，他引：2  

Wang JL  Liu MQ  Han J  Chen WZ  Cong W  Cheng G  Gao YH  Lu YG  Chen JL  Zuo XP  Yan WY  Zheng ZX 《Veterinary microbiology》2007,125(3-4):224-231

The epitopes of the capsid of foot-and-mouth disease virus (FMDV) play important roles in the construction of highly immunogenic subunit vaccines. However few epitopes have been found for FMDV serotype Asia1. In this study we screened for epitopes of the VP1 and VP2 proteins of FMDV serotype Asia1 isolate, YNBS/58. Fragments consisting of amino acids 133-163 of VP1 and amino acids 1-33 of VP2 contained epitopes, and both induced lymphoproliferation in guinea pigs. Only the VP1 fragment induced neutralizing antibodies but the VP2 peptide dramatically increased the neutralizing antibody response induced by the VP1 peptide.  相似文献   

The organic acid profiles in the feces of pigs at Brachyspira hyodysenteriae- or B. pilosicoli-positive farms     

Piao SJ  Tsukahara T  Itoh M  Shiga A  Adachi Y  Ushida K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(4):425-428

We observed a significant difference in the organic acid profile of diarrheal feces between pigs infected with and free from pathogenic spirochetes. Diarrhea and loose feces were collected from growing pigs, held at 15 different commercial farms. A total of 106 samples were measured for organic acid concentration by HPLC and were checked for the presence of B. hyodysenteriae and B. pilosicoli by PCR. B. hyodysenteriae was detected in 3 samples collected from one farm. B. pilosicoli was detected in 5 samples collected from another farm. Lower concentrations of iso-butyrate and iso-valerate were likely associated with development of pathogenic spirochete infection.  相似文献   

犬瘟热、犬细小病毒胶体金二联检测卡研制     

余春明  郑雪莹  范君文  史记  李华斌  郑金来  邓柏林 《中国动物检疫》2023,40(1):128-133

本研究通过细胞融合、间接ELISA方法筛选出4株可稳定分泌犬瘟热病毒(canine distemper virus,CDV)和犬细小病毒(canine parvovirus,CPV)单克隆抗体的杂交瘤细胞株,以此为基础研制了一种可同时检测CDV和CPV的胶体金二联检测卡,并对其特异性、灵敏度及准确性进行了测试。结果显示：4株杂交瘤细胞可稳定传代,分泌的单克隆抗体纯度高,效价均在1:320以上。制备的胶体金二联检测卡特异性强,只与CDV和CPV产生特异性条带,而不与其他犬类病毒反应;灵敏度高,最低检测限可达到10²拷贝/μL;准确性好,与荧光定量PCR结果的符合率高于88.0%,与市面上单一病毒检测卡的符合率高于97.2%。结果表明,本研究制备的CDV、CPV胶体金二联检测卡特异性强、灵敏度高、准确性好,兼具操作简便、肉眼可判读、结果易保存和无需特殊仪器设备等优势,可用于两种疾病的临床快速诊断和大规模检测工作。  相似文献   

一体化微操作器误差分析与建模     

纪华伟  杨世锡  吴昭同 《农业机械学报》2007,38(3):136-140

为评价各种误差对微操作器精度的影响，以一体化超精密微操作器为研究对象，分析了加工误差、机构原理误差、测量误差、环境带来的误差以及驱动误差等造成微操作器精度降低的因素，建立了加工误差的偏微分方程解析模型，并用有限元仿真验证了加工误差模型的准确性。在详细分析误差源的基础上，提出了提高微操作器精度的措施。  相似文献   

从土壤中分离棉枯萎病菌(Fusarium oxysporum f.sp.vasinfetum (Atk) Synder & Hansen)选择性培养基研究     

文学  籍秀琴  陈其煐 《棉花学报》1993,(2)

本文介绍一种用于分离检测土壤中棉枯萎病菌的选择性培养基—植选2号。其成分为:KH_2PO_41g,MgSO_4·7H_2O 0.5g,K_2S_2O_50.2g,KCl0.6g,NH_4NO_3 0.5g,蛋白胨5g,山梨糖10g,蔗糖5g,琼脂20g,蒸馏水1000ml,PCNB 620mg,Oxgall1g,硫酸链霉素300mg,盐酸金霉素75mg。根据棉枯萎病在此培养基上的形态特征,能较容易地识别和检测该病菌。  相似文献   

公平科学公正推进玉米产业发展     

季广德  王守才  孙世贤 《中国种业》2007,(7):21-23

根据《中华人民共和国种子法》（以下简称《种子法》）和农业部《主要农作物品种审定办法》规定，2002年第一届国家农作物品种审定委员会成立。在国家农作物品种审定委员会办公室、农业部种植业管理司和全国农业技术推广服务中心的正确领导下，玉米专业委员会全体委员依法开展了品种审定工作，圆满完成了工作任务。  相似文献   

Building Enviornmental Monitoring System With COM     

WANG Ji feng  PENG Dong bin 《保鲜与加工》2001,(4):48-50

This article presents an implement of component using COM technology for collecting environmental monitoring data in environment monitoring system. The component makes the separation of data collection and data analysis available.Simultaneously by using the interface of connectable object in COM,itmakes the cooperation of the environment monitoring software with other parts of fault management software available,and also the seamless connection.It improves the system's performance by adding extensibility and potential cooperatibility.  相似文献   

Pinning Theory of Fermi Energy and Surface State Measurement of ZnSnO3 Gas Sensitive Material     

LI Ji  LIU Tian-mo  ZHAO Xian-rui  WANG Zhong-chang  LI Jia-ming 《保鲜与加工》2006,(5):55-58

In order to research the state of energy bands and gain and loss of electrons on the surface of ZnSnO_(3),it is put forward that Pinning theory of Fermi energy is applied to development of gas sensitive materials.The relative position between surface state and conduction band on surface of zinc tin complex oxide(ZnSnO_(3)) gas sensitive material has been investigated by utilizing oxidation redox couple,FeCl_(2)/ FeCl_(3),which have high surface state.Fermi energy is pinned at about 0.2 eV below conduction band E_(cs)on the surface of ZnSnO_(3).It has been determined that additives doping ratio,addition concentration and working atmosphere have no effect on this value.Therefore,pinning theory of Fermi energy has been verified by using this method.  相似文献   

Influence of the Flood-Control-Projects on the Hydrodynamic State of the Rivers Confluence in Chongqing     

Chen JingQiu  Zhao WanXing  Ji ZheGang  Wen Cen  Liu XueLan 《保鲜与加工》2005,(4):47-51

Though the Flood-Control-Projects along Yantze River and Jialing River in Chongqing may well improve the urban traffic and construction,it may change the river hydrodynamic characters.There may be two consequences,one is to reduce the size of cross section and the other is to change the river shape,such as the curvature radius.The phenomena of the local backwater and velocity variety are obvious,which result in some problems for the navigation: sediment,transport and so on.In this paper, the study object is the Flood-Control-Projects and Yangtse River and Jialing River,and the aim is to study the influence of the Flood-Control-Projects on the hydrodynamic state of the two rivers.Using the numerical technique,with the two rivers hydrodynamic model the flow field's change of river after completion of Flood-Control-Projects is simulated and the reasons caused by the change are analyzed.  相似文献   

Behavior and Design of High-strength Bolt in Tensile Connection     

CHENG Rui  CUI Ji  LONG Li-ping 《保鲜与加工》2002,(4):25-30

The tensile strength of high strength bolt is affected by many factors, including prying force. The prying force can result in the increment of bolt force, for which the connection failed ahead of time. Several methods are presented for calculating the prying force. In the China's "Code for Design of Steel Structure" (GB50017), there is no formula for calculation of prying force and a factor of 0.8 is considered in formula for tensile strength of bolt for taking the prying force into account. It is proposed that the design value of tensile resistance for high strength bolt should be increased.  相似文献