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Identification of the Darna pallivitta (Moore) pheromone component n-butyl (E)-7,9-decadienoate (E7,9-10:COOn-Bu) has made it possible to investigate communication disruption to control this lepidopteran pest. Conventional communication disruption trials showed marked decreases in the mean number of male moths captured in E7,9-10:COOnBu-treated fields compared with control fields. For traps baited with E7,9-10:COOnBu, percent disruptions were 94.4% and 92.1% for septa (1 g pheromone/ha, 1-wk trial duration) and spirals (6 g pheromone/ha, 8-wk trial duration) respectively. For traps baited with virgin female moths, percent disruption was 73.3% using septa disruptors (1 g pheromone/ha, 1-wk trial duration). Mobile communication disruption using Bactrocera cucurbitae (Coquillett) as carriers for E7,9-10:COOn-Bu was evaluated in the following three areas: fly survivorship, attraction of male moths to treated flies, and moth disruption in a small-scale field trial. Topical application of E7,9-10:COOnBu showed no significant decrease in survivorship at 50 and 80 µg/fly. However, decreased survivorship was observed at 100 µg/fly and linear regression showed E7,9-10:COOnBu dose was significantly correlated with B. cucurbitae survivorship. Traps containing honey–pheromone-fed flies attracted and caught D. pallivitta over a 1-wk period, demonstrating the attractiveness of the carrier. Releasing E7,9-10:COOnBu-fed B. cucurbitae (∼2 g pheromone/ha, 1-wk trial duration) resulted in significantly reduced trap catches in treatment fields compared with control fields on the first 2 d of the field trial. Percent disruptions were 84.7% (day 1) and 56.0% (day 2). These results suggest that both conventional communication disruption and mobile communication disruption have potential to control D. pallivitta.  相似文献   
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The locomotor system requires normal movement for normal function. Movement restrictions of soft tissues and joints stimulate nociceptive pathways in the corresponding vertebral segment. Reflex-induced alterations of sensory and motor neuron activity within the segment lead to detrimental changes within many of the tissues associated with and distant from the area. Manual therapy is utilized in both diagnosis and treatment of tissue and joint movement restrictions. Early recognition and treatment of dysfunction in the equine athlete prevents permanent pathology and facilitates performance excellence.  相似文献   
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Shipping trials were conducted with adult Penaeus setiferus to determine the effect of 24 hour closed shipments on water quality, to evaluate methods of reducing water quality deterioration and to maximize packing density (biomass). Other trials were undertaken with juvenile P. setiferus and adult P. vannamei for comparison. The method utilized 8 L seawater chilled to 18–19 C, inside doubled polyethylene bags held in a Styrofoam box (42 × 42 × 23 cm deep). Gaseous O2 was injected into the water to 8 ppm (for standardization) and into the space above the water. Super-saturation of dissolved oxygen (>20 ppm for 24 hours) had no adverse effect on survival. Initial trials resulted in increased ammonia (from 0.1 to 4–6 ppm NH4-N), increased carbon dioxide (from <2 to 80–100 ppm), increased temperature (from 18–19 to 25–26 C) and decreased pH (from 8.0 to 6.0–6.4). Increases in shipping density (number or biomass of shrimp per L seawater) further intensified water quality deterioration. Carbon dioxide (with concomitant pH decline) is implicated as the major limiting factor during 24 hour closed shipment. Ammonia accumulation was reduced or totally eliminated with addition of AmQuelΘ (Kordon, Inc, Hayward, California), 2 depending on the dosage used. The buffer, Trizma® 8.3 (Sigma Chemical Co, St. Louis, Missouri), 2 limited CO2 buildup and reduced pH decline. Maintenance of low temperature over 24 hours was enhanced with addition of a frozen cold pack placed over the shipping bags. However, the control of ammonia, pH and carbon dioxide by the addition of AmQuel and Trizma did not increase survival, possibly due to toxic effects of the chemicals.  相似文献   
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In many developing countries, incentives for pesticide use often conflict with efforts to ensure the rational and safe use of agrochemicals. This paper analyzes agricultural credit requirements that obligate farmers to use large inputs of pesticides. It discusses the rationale and background for these kinds of agrochemical incentives and gives specific examples of quantities of chemicals required from bank guidelines in Central America. It is argued that this policy is inappropriate for the interests of both farmers and the wider public, for several reasons, which are summarized. Policy changes, such as eliminating the requirements for chemicals and establishing incentives for Integrated Pest Management, are suggested in the final part of the article.  相似文献   
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Willows (Salix spp.) are beneficial as a potential source of renewable energy, riparian barriers and riverbank control, yet are considered invasive weeds when they clog watercourses and lead to erosion and flooding. Interactions between willow rustMelampsora epitea (Thüm.) (Uredinales: Melampsoraceae) and leaf beetlePhratora spp. (Coleoptera: Chrysomelidae) feeding damage have an impact on effective pest management and biological control. The present study investigated the effects of(a) prior mechanical leaf damage on rust development, and(b) rust infection on beetle feeding under laboratory conditions for different time intervals and levels of damage. Willow rust infection significantly reduced the amount of leaf area consumed by beetles. The result was similar when a compatible or an incompatible rust pathotype was sprayed ontoSalix viminalis (L.) ‘Mullatin’ plants. There were no overall significant effects of mechanical damage on rust development, although the lowest level of rust infection was found with the incremental damage treatment. There were, however, differences of significance for leaf position and damage status, with damaged leaves at all positions having fewer pustules and a smaller pustule area than the corresponding undamaged leaves. There was no detectable effect of possible volatile emissions from crushed willow leaves on rust infection and development, although the volatile compoundcis-3-hexenyl acetate significantly reduced pustule diameter and overall pustule area. The results are discussed in terms of the implications for pest management and biological control. Corresponding author http://www.phytoparasitica.org posting April 6, 2003.  相似文献   
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OBJECTIVE: To determine molecular characteristics, antimicrobial susceptibility, and toxigenicity of Clostridium difficile isolates from horses in an intensive care unit and evaluate associations among severity of clinical disease with specific strains of C difficile. DESIGN: Prospective study. ANIMALS: 130 horses. PROCEDURES: Feces were collected from horses admitted for acute gastrointestinal tract disease with loose feces and submitted for microbial culture and immunoassay for toxin production. Polymerase chain reaction assays were performed on isolates for toxins A and B genes and strain identification. RESULTS: Isolates were grouped into 3 strains (A, B, and C) on the basis of molecular banding patterns. Toxins A and B gene sequences were detected in 93%, 95%, and 73% of isolates of strains A, B, and C, respectively. Results of fecal immunoassays for toxin A were positive in 40%, 63%, and 16% of horses with strains A, B, and C, respectively. Isolates in strain B were resistant to metronidazole. Horses infected with strain B were 10 times as likely to have been treated with metronidazole prior to the onset of diarrhea as horses infected with other strains. Duration from onset of diarrhea to discharge (among survivors) was longer, systemic inflammatory response syndromes were more pronounced, and mortality rate was higher in horses infected with strain B than those infected with strains A and C combined. CONCLUSIONS AND CLINICAL RELEVANCE: Horses may be infected with a number of heterogeneous isolates of C difficile. Results indicated that toxigenicity and antimicrobial susceptibility of isolates vary and that metronidazole-resistant strains may be associated with severe disease.  相似文献   
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