Seminal plasma insulin-like growth factor I and total protein concentration in peripubertal period of the Gyr bulls     

Fernando Andrade Souza  Jorge André Matias Martins  Lucas Luz Emerick  Luciane Maria Laskoski  Jair Perez-Osorio  Fabiana Ferreira de Souza  Astrid L. Paredes Cañon  Vicente Ribeiro do Vale Filho 《Reproduction in domestic animals》2021,56(10):1279-1285

This study aimed to evaluate the correlation of seminal plasma IGF-I and total proteins from peripubertal bulls with semen characteristics and onset of puberty. Sixteen dairy Gyr bulls were separated into early (n = 8) and regular (n = 8) groups, according to the onset of puberty. Semen was monthly collected by electroejaculation from 14 to 26 months of age, and the onset of puberty was retrospectively determined (17.0 ± 1.6 and 19.2 ± 1.5 months, to early and regular group, respectively). Five time points were evaluated (day −60, day −30, day 0, day 30 and day 60 days of puberty), being day 0 considered as beginning of puberty. Scrotal circumference and body weight were also assessed. Semen characteristics were evaluated, the seminal plasma was separated by centrifugation and total protein and IGF-I concentrations were determined. There was no difference between groups to concentration of the seminal plasma total proteins, but we found an interaction between group and age. Seminal plasma IGF-I concentrations were not different between early and regular groups; thus, the data were combined for analysis. Combined IGF-I concentrations were positively correlated with sperm motility and concentration, age, body weight and scrotal circumference. Negative correlation was found between IGF-I concentration and total sperm defects. Increased IGF-I was observed in day −30 and day 0 of puberty in early and regular groups, respectively. Seminal plasma total proteins are involved in peripubertal modifications and IGF-I from Zebus dairy bulls can influence the seminal characteristics and the growth factor increase occurs concomitantly with body growth but cannot be used to define puberty bulls earlier.  相似文献   

Chemical composition of the essential oil of Aloysia triphylla under seasonal influence and its anaesthetic activity in fish     

Thaylise Vey Parodi  Luciane Tourem Gressler  Lenise de Lima Silva  Alexssandro Geferson Becker  Denise Schmidt  Braulio Otomar Caron  Berta Maria Heinzmann  Bernardo Baldisserotto 《Aquaculture Research》2020,51(6):2515-2524

Plants are sources of secondary metabolites, and some are rich in essential oils (EOs), which possess a bioactive pharmacological importance as sedatives and anaesthetics that have been experimentally demonstrated in fish. In search of products with lower residual effects and optimization of the production based on the local flora, the properties of the EO of Aloysia triphylla (EOAT) have been investigated. The samples of two years were analysed for the percentage variability of the chemical composition and the totality of the major components according to the season. Limonene, citral isomers (α and β), caryophyllene, caryophyllene oxide and spathulenol were the main constituents of the EOAT throughout these two years. In the second year, caryophyllene predominates over its oxide in all seasons; however, in the first year, it occurs only in autumn and winter. Seasonal pools of EOs were made for the biennium obtaining four samples, and silver catfish (Rhamdia quelen) juveniles were subjected to a concentration range of 20–800 μl/L EOAT in order to assess induction and recovery times of anaesthesia. The relationships between percentages of individual components in EOAT and time to induce deep anaesthesia indicate that its activity may be attributed to the association of several of its components.  相似文献