Thermal preference and tolerance of green abalone Haliotis fulgens (Philippi, 1845) and pink abalone Haliotis corrugata (Gray, 1828)     

Fernando Díaz  Ana Denisse Re  Zarina Medina  Gerardo Re  Gustavo Valdez  & Francisco Valenzuela 《Aquaculture Research》2006,37(9):877-884

The thermoregulatory behaviour of green abalone Haliotis fulgens and pink abalone H. corrugata was investigated. Haliotis fulgens juveniles ranging in wet weight from 3.0 to 3.3 g and from 28.7 to 30.5 mm shell length and of H. corrugata 2.0 g and 25.7 mm in shell length were exposed to 19°C for 30 days in a flow‐through water system. Temperature preference was determined in a horizontal thermal gradient and was found to be 25.4°C for green abalone and 25.0°C for pink abalone. Displacement velocity was 4.3 cm h⁻¹ for H. fulgens and 12.8 cm h⁻¹ for H. corrugata. The optimum temperature for growth calculated for both abalone species was 24.6 and 24.5°C respectively. The critical thermal maxima (CTMax) of H. fulgens and H. corrugata were determined as a measure of thermal tolerance. Abalones were subjected to increasing water temperatures at a rate of 1°C on 30 min until they detached from the substrate. The CTMax at 50% were 33.6 and 32.0°C for green and pink abalone respectively. The results are discussed in relation to site selection and commercial rearing.  相似文献   

Gene expression pattern of digestive and antioxidant enzymes during the larval development of reared Atlantic bluefin tuna (ABFT), Thunnus thynnus L.          下载免费PDF全文

David Mazurais  Denis Covès  Nikos Papandroulakis  Aurelio Ortega  Elisabeth Desbruyeres  Christine Huelvan  Marie Madeleine Le Gall  Fernando de la Gándara  Chantal Louise Cahu 《Aquaculture Research》2015,46(10):2323-2331

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Acute tolerance and histopathological effects of ammonia on juvenile maroon clownfish Premnas biaculeatus (Block 1790)     

Ricardo Vieira Rodrigues  Luis Alberto Romano  Michael Hans Schwarz  Brendan Delbos  Luís André Sampaio 《Aquaculture Research》2014,45(7):1133-1139

This study evaluated the median lethal concentration values (LC₅₀) and the histopathological effects of un‐ionized ammonia (NH₃^‐‐N) on juvenile maroon clownfish Premnas biaculeatus. After 96 h of exposure to different concentrations of ammonia, juveniles were sampled for histopathological evaluation. The 24 and 96 h LC50 values of NH₃^?‐N determined were 1.68 and 0.89 mg L^?1 respectively. Maroon clownfish exposed to different ammonia concentrations displayed histopathological alterations in the gills, kidney, liver and brain. Gill tissue damage included lamellar hyperplasia, lamellar shorting and hyperplasia and hypertrophy of mucous cells. The kidney showed hyperanaemia, enlarged sinusoids within an apparently decreased amount of haematopoietic tissue, oedema on tubular cells and tubular necrosis, and an enlarged Bowman's capsule. The liver presented dilatation of hepatic sinusoids, fatty deposition in hepatocytes and Mallory bodies. Examination of the brain revealed a proliferation of glial cells, and the Virchow‐Robin space indicated a severe perivascular oedema and signs of neuronal suffering with satellitosis. The results of this study indicate that juvenile maroon clownfish are relatively sensitive to ammonia and particular attention must be given to this toxic compound in culture systems.  相似文献   

Chitosan-Based Films with 2-Aminothiophene Derivative: Formulation,Characterization and Potential Antifungal Activity     

Vernica da Silva Oliveira  Meringela Miranda da Cruz  Gabriela Suassuna Bezerra  Natan Emanuell Sobral e Silva  Fernando Henrique Andrade Nogueira  Guilherme Maranho Chaves  Jos Lamartine Soares Sobrinho  Francisco Jaime Bezerra Mendona-Junior  Bolívar Ponciano Goulart de Lima Damasceno  Attilio Converti  dley Antonini Neves de Lima 《Marine drugs》2022,20(2)

In this study, films of chitosan and 2-amino-4,5,6,7-tetrahydrobenzo[b]thiophene-3-carbonitrile (6CN), a 2-aminothiophene derivative with great pharmacological potential, were prepared as a system for a topical formulation. 6CN-chitosan films were characterized by physicochemical analyses, such as Fourier-transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), X-ray diffraction (XRD), and scanning electronic microscopy (SEM). Additionally, the antifungal potential of the films was evaluated in vitro against three species of Candida (C. albicans, C. tropicalis, and C. parapsilosis). The results of the FTIR and thermal analysis showed the incorporation of 6CN in the polymer matrix. In the diffractogram, the 6CN-chitosan films exhibited diffraction halos that were characteristic of amorphous structures, while the micrographs showed that 6CN particles were dispersed in the chitosan matrix, exhibiting pores and cracks on the film surface. In addition, the results of antifungal investigation demonstrated that 6CN-chitosan films were effective against Candida species showing potential for application as a new antifungal drug.  相似文献   

The larval rearing of the marine ornamental crab, Mithraculus forceps (A. Milne Edwards, 1875) (Decapoda: Brachyura: Majidae)     

Gil Penha-Lopes  rew L. Rhyne  Junda Lin  & Luis Narciso 《Aquaculture Research》2005,36(13):1313-1321

The goal of this study is to develop a larviculture protocol for Mithraculus forceps, a popular marine aquarium species. Different temperatures (25±0.5°C and 28±0.5°C), stocking densities (10, 20, 40 and 80 larvae L^?1), prey densities (newly hatched Artemia of 1, 4, 7 and 12 nauplii mL^?1) and metamorphosis to crab conditions (Systems A and B) were tested. The best survivorship and faster development were obtained when the larvae were reared at a density of 40 larvae L^?1 for 7 days post hatching (DPH) in System A, at 28°C and fed with 7 mL^?1 of newly hatched Artemia nauplii. After 7 DPH all the megalopa were moved to System B and the same temperature and prey density were maintained. At the end of the experiment, 12 DPH, survivorship of 74.1±4.8% was obtained.  相似文献   

Effect of dietary carbohydrate-to-lipid ratios on growth, lipid deposition and metabolic hepatic enzymes in juvenile Senegalese sole (Solea senegalensis, Kaup)   总被引：3，自引：0，他引：3  

Jorge Dias  Rebeca Rueda-Jasso  Stephan Panserat  Luis E C da Conceição  Emidio F Gomes  & Maria T Dinis 《Aquaculture Research》2004,35(12):1122-1130

A study was undertaken to determine the effect of various dietary carbohydrate‐to‐lipid ratios on growth performance, whole‐body composition and tissue lipid content in Senegalese sole (Solea senegalensis) juveniles. Data on the dietary regulation of key hepatic enzymes of the lipogenic and glycolytic pathways (glucose‐6‐phosphate dehydrogenase, G6PD; malic enzyme, ME; fatty acid synthetase, FAS; pyruvate kinase, PK and glucokinase, GK) were also generated. Four isonitrogenous (crude protein: 52% dry matter (DM)) diets were formulated to contain one of two lipid levels (11% and 21% DM). Within each dietary lipid level, the nature of the carbohydrate fraction (raw or extruded peas) was varied. Triplicate groups of 54 sole (initial body weight: 23.6±1.2 g) were grown in recirculated seawater over 67 days. Fish were fed using automated feeders. At the end of the study, whole‐body, liver, viscera and muscle samples were withdrawn for analyses. During the experimental period, the mean fish weight about doubled in all treatments. No significant differences were found in growth performance (ranging from 1.1% to 1.4% body weight day^?1) among dietary treatments. High‐fat diets increased whole‐body fat content. Similarly, daily fat gain ranged from 0.54 to 0.78 g kg^?1 day^?1 and highest values were found in fish fed high‐lipid diets. Dietary treatments also affected tissue lipid content (liver, viscera and muscle), with highest values in fish fed high‐fat diets. The nature of dietary carbohydrates had little influence on performance criteria, but affected tissue lipid deposition. The activities of G6PD, ME and FAS were depressed by elevated levels of dietary lipid, confirming the inhibitory effect of dietary fats on lipid biosynthesis. At both dietary lipid levels, ME and FAS activities were little affected by dietary carbohydrate. Activities of PK and GK were not affected by the starch level of the diets. In Senegalese sole juveniles, the lipogenic pathway is more susceptible to modulation by dietary means (particularly through lipid intake) than the glycolytic pathway.  相似文献   

Effects of beneficial bacteria on biomass,photosynthetic parameters and cell composition of the microalga Muriellopsis sp. adapted to grow in seawater     

Paola Marticorena  Leonel Gonzalez  Carlos Riquelme  Fernando Silva Aciares 《Aquaculture Research》2020,51(9):3609-3622

The present study evaluated the effects of five bacterial isolates from the microalga Muriellopsis sp. on the microalgal biomass concentration (g/L), specific growth rate (K), photosynthetic quantum yield (QY), non‐photochemical quenching (NPQ), maximum relative electron transport rates (rETR max) and levels of nutritional components such as carbohydrates, lipids, proteins and carotenoids in Muriellopsis sp. cultivated in seawater under indoor and outdoor conditions. The bacterial strains were Microbacterium sp. Dom 1; Pseudomonas sp. Dom2; Microbacterium sp. Dom 3; Rhizobium sp. Dom 4; and Dietzia sp. Dom 5, as well as the Pseudoalteromonas sp. (SLP1), a bacterium known to promote the growth of microalgae. At the laboratory level, inoculation of the strains SLP1, Dom 1, Dom 3 and Dom 5 resulted in higher specific growth rates and biomass of the microalga. Subsequently, the best results were obtained with a combination of the selected bacterial strains (Dom1, Dom 3, Dom5 and SLP1) under outdoor culture conditions improved the biomass, proteins, carbohydrates and total carotenoids in 22.12%, 48.28%, 19,25% and 48.27%, respectively, compared to cultures without the incorporation of the selected bacteria (control), while no effects were observed on the photosynthetic parameters. Thus, it was demonstrated that positive associations of the selected bacterial strains played an important role in the production of Muriellopsis sp. acclimatized to grow in seawater. This is of crucial importance, especially in desert areas, where solar radiation is high and freshwater is a limited resource.  相似文献   

Influence of light intensity on biofilm formation and the performance of pink shrimp Farfantepenaeus paulensis juveniles reared in cages     

Wilson Wasielesky Jr  Paulo César Abreu  Luis Henrique Poersch  Fabiano Thompson  Eduardo Luis Cupertino Ballester 《Aquaculture Research》2012,43(5):706-712

A reduction in light intensity may increase shrimp activity, although it may also negatively affect the development of photoautotrophic organisms present in biofilm, an important natural food source for shrimp. This experiment evaluated the influence of light intensity on biofilm development and on the growth of cultured Farfantepenaeus paulensis juveniles. Six cages were deployed in a shrimp culture pond and stocked with 60 juveniles (0.72 ± 0.03 g). Three cages were randomly chosen and covered with five layers of polyamide net to reduce light incidence (shaded treatment), while the other three cages were not covered (control treatment). Biofilm chlorophyll a and microorganism abundance, as well as shrimp growth, were monitored during the experiment. Chlorophyll a concentration and the abundance of bacteria and flagellates were significantly lower in the shaded treatment (P<0.05). After 75 rearing days, shrimp stocked in control treatment achieved significantly higher (5.98 g) (P<0.05) mean weight than shrimp reared in shaded treatment cages (5.13 g). Similarly, the biomass produced was also significantly higher (P<0.05) in the control treatment (322.92 g) than in the shaded treatment (287.31 g). The results of this study demonstrate that light intensity has a huge influence on shrimp performance mainly due to the increase in natural food availability.  相似文献   

Phenotypical parameters as a tool to evaluate the immunostimulatory effects of laminarin in Oncorhynchus mykiss          下载免费PDF全文

Byron Morales‐Lange  Jörn Bethke  Paulina Schmitt  Luis Mercado 《Aquaculture Research》2015,46(11):2707-2715

Laminarin is a β‐glucan from the brown algae Laminaria digitata (J.V. Lamour), which can activate innate immune responses. With the aim of developing a strategy to evaluate specific phenotypical parameters of the effects of laminarin in trout innate immunity, we (i) fed fish with laminarin‐supplemented diet (0.2 g kg^?1 day^?1) for 21 days and (ii) treated fish with a single dose of intraperitoneal injected laminarin (0.08 g kg^?1 fish). The evaluation of cellular and humoral immune parameters was established at phenotypic level by the phagocytic activity of headkidney macrophages and detection of inflammatory cytokines in head kidney and gill tissue by indirect ELISA. Results showed that both delivery methods of laminarin produce an increase in the phagocytic activity in head kidney macrophages and a significant increase in the production of TNFα and IL‐8 in gill tissue at day 21. Additionally, some of these parameters were significantly correlated (P < 0.025), which places them as new potential combined markers to detect activation of trout defense mechanisms by laminarin. These results highlight the importance of developing new protocols to quantitate immune parameters, in order to evaluate immunostimulants in fish farming.  相似文献   

Physiological and sanitary condition of the white clam Dosinia ponderosa collected from a coastal area impacted by shrimp farm effluent     

Marcel Martínez-Porchas  Susana Maria Scheuren-Acevedo  Luis Rafael Martínez-Córdova  Teresa Gollas-Galvan  Ramon H. Barraza-Guardado  Fernando Enríquez-Ocaña  Edilmar Cortés-Jacinto  Marco A. Porchas-Cornejo 《Aquaculture International》2016,24(1):243-256

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