Inhibitory Effect of Grapefruit Juice on the Genotoxic Damage Induced by Ifosfamide in Mouse     

Alvarez-González I  Madrigal-Bujaidar E  Sánchez-García VY 《Plant foods for human nutrition (Dordrecht, Netherlands)》2010,65(4):369-373

The consumption of grapefruit juice (GJ) has been associated with various activities potentially beneficial for human health, including protection against the DNA damage produced by various compounds. In the present report, we evaluated the capability of this juice to inhibit the rate of micronucleated polychromatic erythrocytes (MNPE) and sister chromatid exchanges (SCE) induced by the antineoplastic alkylating agent ifosfamide (IF). We tested the effect of administering 100, 500, and 1000 mg/kg of GJ in mouse and obtained the following results: a) with the high dose, a reduction of 72% in the rate of SCE induced by IF; b) a mean reduction of 65.3% in the rate of MNPE compared with the damage induced by IF at 48 h post-administration; c) no modification induced by GJ either on the cellular proliferation kinetics or in the mitotic index; and d) neither induction of bone marrow cytotoxicity by GJ nor a protective effect of the juice against the cytotoxicity induced by IF. These data showed a significant inhibitory effect of GJ against the chromosome damage induced by the tested agent; they also suggest the relevance of carrying out studies to clarify the involved mechanism of action as well as to determine the cancer chemopreventive capacity of the juice.  相似文献   

Metabolic and histological parameters of silver catfish (Rhamdia quelen) exposed to commercial formulation of 2,4-dichlorophenoxiacetic acid (2,4-D) herbicide     

R. Cattaneo  R. Spanevello  L. Luz  M.B. Fonseca  B. Clasen 《Pesticide biochemistry and physiology》2008,92(3):133-137

The objective of this study was to investigate the effects of commercial formulation of herbicide 2,4-D on metabolic parameters, acetylcholinesterase (AChE) activity and liver histological evaluation of silver catfish (Rhamdia quelen) exposed for 96 h. AChE activity increased in brain (600 and 700 mg L⁻¹) and decreased in all concentrations tested in muscle tissue. Hepatic glycogen was reduced after 2,4-D exposure ranging from 47.67% (400 mg L⁻¹) until 59.3% (700 mg L⁻¹). Hepatic tissue showed lactate reduction at all 2,4-D concentrations tested and glucose was reduced only at 700 mg L⁻¹. In the highest concentration tested hepatic glycogen and glucose reduced instead plasma glucose levels increased. White muscle tissue showed glycogen reduction in fingerlings exposed to all herbicide concentrations and glucose reduction at 700 mg L⁻¹. Muscle lactate levels increase at all 2,4-D concentrations tested. Vacuolation of hepatocytes and changes in its arrangement cords were observed by histologic analysis in group treated with 700 mg/L of 2,4-D. These results suggest that silver catfish exposed to concentrations of 2,4-D near of CL₅₀ showed metabolic and histological response to compensate some stress caused by herbicide exposure. Taken together parameters measured can be used as biomarkers to monitor herbicide contaminated water.  相似文献   

Coelioscopy technique for gender in surubim hybrid Pseudoplatystoma corruscans × Pseudoplatystoma reticulatum          下载免费PDF全文

Glauco Vinício Chaves  Valentim A Gheller  Edgar de Alencar Teixeira  Ronald Kennedy Luz 《Aquaculture Research》2015,46(4):1007-1012

  相似文献   

Carcass characteristics and meat quality of lambs fed babassu cake (<Emphasis Type="Italic">Orbignya speciosa</Emphasis>) as a replacement for elephant grass silage     

Janaina Barros Luz  Kaliandra Souza Alves  Rafael Mezzomo  Ernestina Ribeiro dos Santos Neta  Daiany Íris Gomes  Luis Rennan Sampaio Oliveira  Josiane Costa Silva  Francisco Fernando Ramos de Carvalho 《Tropical animal health and production》2017,49(1):113-119

The study aimed to evaluate the effect of the partial replacement of elephant grass silage with babassu cake (Orbignya speciosa) on the carcass characteristics and meat quality of feedlot lambs. Forty-five castrated male Santa Ines sheep (19.08?±?0.41 kg) approximately 4 months old were distributed in a completely randomized design, with five treatments 0.0, 12.5, 25.0, 37.5 and 50 % (%DM) replacement of babassu cake with silage forming isoproteic diets formulated at a ratio of 40 % roughage to 60 % concentrate. All of the studied animals were slaughtered at the end of the experiment. The liver weights and yields increased with the inclusion of babassu cake. The weight of the shoulder increased from 2.31 to 2.61 kg, while the loin yield decreased from 7.38 to 6.64 % with the inclusion of babassu cake, both linearly. The body length, thoracic perimeter, rump perimeter and carcass compactness index showed high and positive correlations with the hot and cold carcass weights. The myofibrillar fragmentation index decreased linearly as a function of the inclusion level of babassu cake, but other quality variables were not affected. The replacement of up to 50 % of the elephant grass silage with babassu cake in the diet of lambs does not cause negative effects on carcass characteristics or meat quality.  相似文献   

Expression levels of mRNA-encoding PDGF receptors in goat ovaries and the influence of PDGF on the in vitro development of caprine pre-antral follicles     

Brito IR  Lima IM  Saraiva MV  Silva CM  Magalhães-Padilha DM  Araújo VR  Barreto Luz V  Barbalho Silva AW  Campello CC  Silva JR  Figueiredo JR 《Reproduction in domestic animals》2012,47(5):695-703

The aims of this study were to investigate the expression levels of mRNA for platelet-derived growth factor (PDGF) receptors (PDGFR-α and -β) in caprine follicles at different developmental stages and to evaluate the influence of PDGF on the in vitro development of pre-antral follicles. For this, goat primordial, primary and secondary follicles, as well as small (1-3 mm) and large (3-6 mm) antral follicles, were obtained, and PDGFR-α and -β mRNA levels were quantified by real-time PCR. Furthermore, pre-antral follicles (≥ 200 μm) were isolated from goat ovaries and cultured for 18 days in α- minimum essential medium supplemented with PDGF at 50 or 100 ng/ml, containing or not FSH. Real-time PCR showed highest PDGFR-α mRNA levels in secondary follicles, while PDGFR-β mRNA levels were highest in primary follicles onwards. Both receptors showed higher mRNA levels in granulosa/theca cells from small and large antral follicles than in their corresponding cumulus-oocyte complexes. In culture, the percentage of antrum formation was significantly higher in 100 ng/ml PDGF compared with the same PDGF concentration associated with FSH. After 18 days, PDGF in both concentrations associated with FSH promoted follicular growth significantly higher than the control. Moreover, the addition of FSH to 50 ng/ml PDGF positively influenced the follicular growth when compared with the same PDGF concentration in the absence of FSH. In conclusion, PDGF is important for early goat folliculogenesis, because the presence of PDGFR-α and -β mRNA was detected in all follicular categories, and PDGF associated with FSH stimulated the growth of goat pre-antral follicles isolated and cultured in vitro.  相似文献   

QTL mapping and identification of corresponding genomic regions for black pod disease resistance to three <Emphasis Type="Italic">Phytophthora</Emphasis> species in <Emphasis Type="Italic">Theobroma cacao</Emphasis> L.     

M. A. Barreto  J. R. B. F. Rosa  I. S. A. Holanda  C. B. Cardoso-Silva  C. I. A. Vildoso  D. Ahnert  M. M. Souza  R. X. Corrêa  S. Royaert  J. Marelli  E. S. L. Santos  E. D. M. N. Luz  A. A. F. Garcia  A. P. Souza 《Euphytica》2018,214(10):188

The cacao tree (Theobroma cacao L.) is a species of great importance because cacao beans are the raw material used in the production of chocolate. However, the economic success of cacao is largely limited by important diseases such as black pod, which is responsible for losses of up to 30–40% of the global cacao harvest. The discovery of resistance genes could extensively reduce these losses. Therefore, the aims of this study were to construct an integrated multipoint genetic map, align polymorphisms against the available cacao genome, and identify quantitative trait loci (QTLs) associated with resistance to black pod disease in cacao. The genetic map had a total length of 956.41 cM and included 186 simple sequence repeat (SSR) markers distributed among 10 linkage groups. The physical “in silico” map covered more than 200 Mb of the cacao genome. Based on the mixed model predicted means of Phytophthora evaluation, a total of 6 QTLs were detected for Phytophthora palmivora (1 QTL), Phytophthora citrophthora (1 QTL), and Phytophthora capsici (4 QTLs). Approximately 1.77–3.29% of the phenotypic variation could be explained by the mapped QTLs. Several SSR marker-flanking regions containing mapped QTLs were located in proximity to disease regions. The greatest number of resistance genes was detected in linkage group 6, which provides strong evidence for a QTL. This joint analysis involving multipoint and mixed-model approaches may provide a potentially promising technique for detecting genes resistant to black pod and could be very useful for future studies in cacao breeding.  相似文献   

Metal uptake by wetland plants: implications for phytoremediation and restoration     

Carmen Pérez-Sirvent  Carmen Hernández-Pérez  María José Martínez-Sánchez  Mari Luz García-Lorenzo  Jaume Bech 《Journal of Soils and Sediments》2017,17(5):1384-1393

  相似文献   

A proposal for avoiding fresh-weight measurements when reporting the effect of plant growth-promoting (rhizo)bacteria on growth promotion of plants     

Yoav?BashanEmail author  Ping?Huang  Joseph?W.?Kloepper  Luz?de-Bashan 《Biology and Fertility of Soils》2017,53(1):1-2

  相似文献   

Evidence that fresh weight measurement is imprecise for reporting the effect of plant growth-promoting (rhizo)bacteria on growth promotion of crop plants     

Ping?Huang  Luz?de-Bashan  Trae?Crocker  Joseph?W.?Kloepper  Yoav?BashanEmail author 《Biology and Fertility of Soils》2017,53(2):199-208

Eight greenhouse experiments were performed to compare the effect of seven plant growth-promoting (rhizo)bacteria (PGPR/PGPB) on fresh and dry weights of four crop plants. This has been done to validate if fresh weight measurements of plant variables can serve as reliable values when reporting the effect of these bacteria on plant growth. These experiments show that the growth promotion effects by the tested PGPR/PGPB, including Bacillus amyloliquefaciens GB03, Bacillus subtilis IN-937B, Bacillus altitudinis INR7, and Pseudomonas mandelii 89B-27 in corn and cucumber and Azospirillum brasilense Cd, A. brasilense Sp 245, and Azospirillum lipoferum Br 17 in pepper and tomato, varied significantly between fresh and dry weights of shoot, root, and/or whole plant in the repeated greenhouse experiments. These results support our hypothesis that using fresh weight determination for assessing plant growth promotion by beneficial bacteria is inherently faulty. Therefore, it is recommended that dry weight determination rather than fresh weight determination is used for plant growth promotion tests.  相似文献   

Evaluation of Food Conservation Technologies for Potato Cubes     

Paola Ceroli  Luz Milagros Garcia Procaccini  Graciela Corbino  Maria Cristina Monti  Marcelo Huarte 《Potato Research》2018,61(3):219-229

Potato cubes of cultivar Innovator were subjected to different food conservation treatments: osmotic dehydration and antioxidants (OD + A), edible coating (E), edible coating with osmotic dehydration and antioxidants (EC + OD + A), immersion in antioxidants (A) and microwave blanching (B). The quality of the products was evaluated by measuring dry matter content, colour, browning index, total phenols and antioxidant capacity and polyphenol oxidase (PPO) activity. Treatments with A resulted in a lower a* colour parameter (red to green) but increased total phenols and antioxidant activity. Those treatments with OD and A together resulted in even larger increases in total phenols and antioxidant activity and also reduced PPO to negligible activity. OD increased dry matter content in OD + A treatments. B increased the browning index (BI) and colour parameters a* and ΔE (total change). As a consequence, potato cubes treated with osmotic dehydration and antioxidants had improved quality characteristics and these treatments are appropriate for minimally processed vegetables.  相似文献