Antibody directed against Marek's disease-associated tumor surface antigen can be eluted from Marek's disease tumor cells.     

H K Pradhan  G C Mohanty  W Y Lee  B Patnaik 《Veterinary immunology and immunopathology》1991,29(3-4):229-238

Antibody directed against Marek's disease-associated tumor surface antigen (MATSA) was eluted from tumor cells of lymphomas and peripheral blood lymphocytes that were isolated from Marek's disease virus-infected chickens. Feather follicular Marek's disease virus (MDV) antigen could not be demonstrated with this antibody by indirect immunofluorescent (IF) staining. Monoclonal antibody directed against MATSA could completely block the activity of eluted antibody and vice versa. By indirect IF staining using eluted antibody and fluorescein isothiocyanate (FITC) labelled antichicken globulin conjugate. MATSA-bearing cells were detected in MDV infected and herpes virus of turkey (HVT) vaccinated birds. Blocking of immunoglobulin molecules present on B-cells by anti-chicken globulin is critical in this test.  相似文献   

STUDIES ON THE MANAGEMENT OF CARDIOPULMONARY EMBARRASSMENT DUE TO THIOPENTONE ANAESTHESIA IN THE EQUINE     

D. Sharifi  J. Singh  P.K. Peshin  A.P. Singh 《Veterinary anaesthesia and analgesia》1991,18(Z1):309-314

Cardio pulmonary embarrassment was induced with thiopentone sodium in 35 healthy adult donkeys divided in to seven groups of five animals. “To effect” anaesthetic dose was (12.72 ± 0.69 mg/kg) which caused transient hypotension, tachycardia and hypoxemia. A stable fall in MAP 1. 6.77 ± 0.287 Kpa (50.78 ± 2.15 mmHg) and a flat EEG were considered to indicate acute circulatory insufficiency due to thiopentone overdose.  相似文献   

EFFECTS OF URINASTATIN (HUMAN URINARY TRIPSIN INHIBITOR) AGAINST ENDOTOXIN SHOCK IN DOGS     

M. Tagawa  S. Okano 《Veterinary anaesthesia and analgesia》1991,18(Z1):335-346

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DOES KETAMINE PROVIDE ADEQUATE VISCERAL ANALGESIA WHEN USED ALONE OR IN COMBINATION WITH ACEPROMAZINE,DIAZEPAM, OR BUTORPHANOL IN CATS?     

D.C. Sawyer  R.H. Rech  R.A. Durham 《Veterinary anaesthesia and analgesia》1991,18(Z1):381-381

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A Comparison of Arterial and Lingual Venous Blood Gases in Anethetized Dogs     

A.E. Wanger DVM  MS    W.W. Muir III  DVM  Phd R.M. Bednaraki DVM  MS 《Journal of Veterinary Emergency and Critical Care》1991,1(1):14-18

The purpose of this study was to determine whether lingual vanous blood gas samples reflect arterial acid-base gas status in anethetized dogs. Heparinized blood samples were drawn simultaneously from the lingual vein and a peripheral artery in 50 anestheized dogs that were clinical surgical patients, as well as from four experimental dogs in which hemorrahaic shock was being studied. Blood pH, oxygen tension (PO₂), and bicarbonate (HCO_3-)) from the two sources in clinical patients showed significant liner correlation, although arterial PO₂)(PaO₂)) tended to be approximately 110mm Hg higher than lingual venous PO₂). During hemorrahgic shock, however, PaO₂) and PaCO₂) were significantly different from lingual venous PO₂) and PCO₂), Lingula venous blood gas analysis may be useful in assessing acid-base and blood gas status in routline cases, but should not be relied upon in dogs with low cardiac output or poor perfusion.  相似文献   

亚麻工艺长相关性状的通径分析     

聂征  陈甫堂  李春霞 《中国麻业》1993,(2)

本试验以6个普通亚麻品种为材料,进行工艺长相关性状的通径分析。结果表明,5个相关性状对工艺长度影响大小顺序为:单株粒重>单株粒数>千粒重>株高>主茎分枝数。降低单株粒重是提高工艺长度的主要途径。在千粒重、单株粒重相对稳定的类型中,选择植株较高、单株粒数较多者,或在株高、单株粒数相对稳定的类型中,选择千粒重较高者是提高工艺长度的重要途径。减少主茎分枝数亦可有效提高工艺长度,但作用较小。  相似文献   

Characterization of and radioimmunoassay for canine thyroglobulin     

C. P. Verschueren  P. J. Selman  J. J. M. de Vijlder  J. A. Mol 《Domestic animal endocrinology》1991,8(4):509-519

Canine thyroglobulin (cTg) has been isolated and purified. It has similar electrophoretic patterns as Tg from other mammalian species. The main fraction had a MW of 660,000, whereas also fractions of a MW of approximately 1,300,000 (dimer) and 330,000 (subunit) were present. The iodine content was 0.8 to 1.0 % (w/w). cTg did not cross-react with antibodies against human Tg to a degree that would allow the use of a radioimmunoassay for human Tg for the determination of cTg in serum or plasma. Therefore a polyclonal antiserum was raised against cTg and a homologous radioimmunoassay was developed, which was sensitive (0.4 μg/l) and specific (cross-reactivity in cTg assay of human Tg, goat Tg, T₄, T₃, and DIT < 0.01 %).

Plasma Tg levels in normal dogs of both sexes and aged 3–15 years amounted to 192 ± 73 μg/l (mean ± SD, n=30). There was no relation between plasma Tg and T₄ levels.  相似文献   

Fluid accumulation in mouse ligated intestine inoculated with the vascular permeability factor produced by Bacillus cereus.   总被引：1，自引：0，他引：1  

K Shinagawa  K Sato  H Konuma  N Matsusaka  S Sugii 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1991,53(2):167-171

Partially purified vascular permeability (VP) factor (VPF) of Bacillus cereus induced fluid accumulation in the ligated intestinal loops of mouse (MIL) and rabbit (RIL), suggesting that the VP activity may correlate with fluid accumulation in ligated intestinal loops of these animals. Fluid accumulation was observed at 6-8 hr in 55-67% of mouse intestinal loops inoculated with 40-50 immunodiffusion units (IDU) of partially purified VPF, whereas it was found at 2 hr in all loops with 400-600 IDU of partially purified VPF. In rabbit intestinal loops with 120-190 IDU of partially purified VPF, fluid accumulation was observed at 6 hr. From these findings, VPF produced by B. cereus can be easily detected in both MIL and RIL. The intestinal tissue of mouse intestinal loops was histopathologically damaged at different concentrations of the VPF to induce fluid accumulation. With 50 IDU of partially purified VPF, severe edema was found in the laminia proprial layer and submucosa. With 600 IDU of partially purified VPF, on the other hand, severe necrosis in the surface epithelium of villus and laminia proprial layer, and hyperemia in the submucosa were observed, suggesting that partially purified VPF may be cytotoxic and/or intestinecrotic.  相似文献   

影响啤酒大麦种子发芽整齐度的因素     

殷琛  张国平 《大麦与谷类科学》2001,(1):20-22

综述影响啤酒大麦种子发芽整齐度的因素 ,着重介绍了品种、籽粒整齐度 (包括粒重和粒位 )对发芽整齐度的影响 ,旨在为培育优质啤酒大麦提供借鉴。  相似文献   

Atlas en Couleur de Cytologie du Cancer Chez le Chien et le Chat (Color Atlas of Cancer Cytology of the Dog and Cat)     

Patricia M. McManus  VMD  PhD  Diplomate ACVP 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2001,30(2):97-98

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