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ABSTRACT: Genetic variation of the fungal parasite Pythium porphyrae ,causative organism of red rot disease of Porphyra , isolatedfrom Asan, Mokpo, Pusan and Wando in Korea, and from Aichi, Fukuokaand Miyagi in Japan was investigated by random amplified polymorphicDNA (RAPD) cluster analysis. The total 67 RAPD markers were generatedfrom 38 isolates by RAPD-polymerase chain reaction (PCR) using arbitraryprimers consisting of 10 nucleotide sequences and 33 of them indicatedpolymorphisms. The dissimilarity coefficients calculated from theRAPD banding patterns ranged from 0.0010 to 0.6983. The dendrogramgenerated by the unweighted pair-group method using arithmetic averagesshowed that the 38 isolates were classified into three clusters(Groups 1, 2 and 3). Group 1 consisting of two isolates from Miyagiwas separated from all other isolates by a genetic distance of 0.6983.Groups 2 and 3 containing the majority of the isolates were branchedon genetic distance of 0.3957. These two clusters subdivided intofour and three subclusters, respectively, which were apparentlyassociated with geographic origins of the isolates. Interestingly,the isolates from Asan of Korea were close to Japanese isolatesrather than Korean isolates on genetic diversity. In addition, thegenetic distances of intra-isolates from Japan were higher thanthose from Korea. 相似文献
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FAJRI ANUGROHO MAKOTO KITOU FUJIO NAGUMO KAZUTOSHI KINJO YOSHIHIRO TOKASHIKI 《Weed Biology and Management》2009,9(1):63-71
Hairy vetch ( Vicia villosa ), as a winter cover crop, can be used to suppress weeds in subtropical regions, as well as temperate regions. Information on the potential biomass growth of hairy vetch for weed control and nutrient accumulation is not available in subtropical regions. Hairy vetch was sown in November 2004, and October, November, and December 2005. The wide-ranging cultivation period of hairy vetch indicated that it could be used in various cropping systems. It showed a higher biomass and nutrient accumulation when grown in subtropical Okinawa, Japan. Moreover, the biomass, and fixed carbon and magnesium (Mg) uptake in the above-ground parts of hairy vetch were found to be the highest in late May, with the highest nitrogen (N), potassium, and calcium uptake in mid-April and phosphorus (P) uptake in late March. Meanwhile, in the underground parts of the plant, they were highest in early May, except for the P and Mg uptake, which were highest in mid-April. According to the sowing date, the biomass and nutrient uptake of hairy vetch that was harvested in February were higher when sown in October. Similarly, when harvested in March, the biomass and nutrient uptake were higher when sown in October or November. In April, they were higher when sown in November or December. Hairy vetch has the potential to effectively suppress weeds in the winter and the spring seasons related to its sufficient biomass during the growing seasons. However, both the sowing and harvesting times of hairy vetch should be considered with reference to the cropping system; the subsequent crop will be sown to meet the N requirement. 相似文献
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土壤微生物种群类型,对土壤质量和作物生长具有重要影响,研究寡糖对土壤微生物种群的影响特征,有助于正确、高效及安全使用寡糖。本研究利用人工气候室进行土壤培养,土壤施加50mg·L−1的壳寡糖（CSOS）和纤维寡糖（COS）溶液,以清水（CK）为对照处理,培养6d后取样,利用高通量测序技术,分析土壤微生物群落结构组成及多样性分布特征。结果表明：壳寡糖（CSOS）和纤维寡糖（COS）处理均显著改变细菌、真菌的群落结构,提高细菌的物种观测数。变形菌门（Proteobacteria）、酸杆菌门（Acidobacteria）、放线菌门（Actinobacteria）、绿弯菌门（Chloroflexi）、芽单胞菌门（Gemmatimonadetes）和拟杆菌门（Bacteroidetes）为优势细菌门,子囊菌门（Ascomycota）、担子菌门（Basidiomycota）和被孢霉门（Mortierellomycota）为优势真菌门。通过组间群落组成比较分析可知,壳寡糖（CSOS）和纤维寡糖（COS）处理均不同程度降低酸杆菌门（Acidobacteria）的相对丰度,增加变形菌门（Proteobacteria）、放线菌门（Actinobacteria）、芽单胞菌门（Gemmatimonadetes）、壶菌门（Chytridiomycota）以及有益菌属溶杆菌属（Lysobacter）、硝化螺旋菌属（Nitrospira）、Haliangium、芽球菌属（Blastococcus）和链霉菌属（Streptomyces）的相对丰度,但与纤维寡糖（COS）相比,壳寡糖（CSOS）处理微生物群落组成的变化幅度更大。此外,壳寡糖（CSOS）和纤维寡糖（COS）处理在调节土壤微生物群落结构上存在一定差异。其中,壳寡糖（CSOS）处理有益菌属Talaromyces的相对丰度增加195%,纤维寡糖（COS）处理有益菌属假单胞菌属（Pseudomonas）的相对丰度增加215%。综上,壳寡糖和纤维寡糖处理均能优化土壤微生物群落的结构组成,其调控差异性有助于理解不同寡糖的调控机制,推动寡糖的应用与推广。 相似文献
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MINORU FUNAKI JUNJI YAMAMOTO MAKOTO NISHIZAWA TAKASHI YAMAGISHI 《Fisheries Science》2002,68(5):1151-1154
The isolated mantle of scallop ( Patinopecten yessoensis ) was shown to adsorb minerals (Zn, Ca, Mg, Fe, Cu, Ni and Co) to more than 1% upon treatment with mineral solution. The amounts of adsorbed Zn, Ca and Mg were 44.3, 47.4 and 15.5 mg/g dry weight, when the mantle was treated with 5% ZnSO4 , CaCl2 and MgSO4 solutions at room temperature, respectively. The amounts of adsorbed Zn, Ca and Mg were dependent on the concentration of mineral solution used. This is the first report showing that the isolated mantle adsorbs nutritionally important minerals such as Ca, Mg and Fe. This finding may lead to the development of new mineral-enriched food supplements. 相似文献
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Two of the many benefits of including legume cover crops in a fallow cropping system are weed suppression and increased soil organic N. This study was conducted in order to examine the effect of hairy vetch (Vicia villosa) on weed growth and soil N through a fallow period in subtropical Okinawa, Japan. Together with an untreated control plot, hairy vetch was grown from November 2007 to April 2008 and subsequently incorporated into the soil. The weeds were allowed to develop uncontrolled for 2 months until the time when a cash crop would be planted. In the period after the incorporation of hairy vetch, the weed biomass in the hairy vetch (HV)+ plot was more than threefold greater than that in the HV? plot. Hairy vetch had a high tendency to uptake N in April before the incorporation of hairy vetch; the N uptake by the weeds was lower in the HV+ plot than in the HV? plot. After the incorporation of hairy vetch, the N uptake by the weeds in the HV+ plot was two‐to‐six times higher than that by the weeds in the HV? plot. The C/N ratios of hairy vetch and the weeds in the HV+ plot were lower than those of the weeds in the HV? plot. More than half of the hairy vetch residue and N in the residue had decomposed by 28 days after incorporation. The content of both the NH4‐N and NO3‐N in the HV+ soil was higher than that in the HV? soil. Moreover, the amount of NO3‐N increased during the growing period of hairy vetch and decreased after the incorporation of hairy vetch. The fallow soil into which hairy vetch had been incorporated displayed a greater weed biomass and resulted in a higher inorganic N content than the soil that had not supported hairy vetch. 相似文献
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KAE MIYAZAWA HIROYUKI TSUJI MAKOTO YAMAGATA HIROSHI NAKANO TOMOMI NAKAMOTO 《Weed Biology and Management》2004,4(1):24-34
Weed flora in a crop rotation of soybean, sugar beet, and spring wheat were studied under combinations of conventional and reduced tillage, biocide application, and chemical fertilization to investigate whether any combination of conservational practices offers the potential to increase weed community diversity while maintaining weed biomass at an acceptable level. Weed density increased under reduced biocide application. Weed density and size increased under manure compost application (with reduced chemical fertilization) because of weed seed introduction. Weed emergence from seeds dispersed in the previous year was greater under reduced tillage. Two-year individuals increased under a combination of reduced tillage, reduced biocide application and manure compost application. These increases in weed population density and size under conservational practices were yet not consistent; rather, they showed an annually fluctuating trend. In terms of weed diversity, species richness and Shannon's diversity index were higher under manure application because of the introduction of new species and probably as a result of suppression of weed growth and reproduction, which can reduce the chance of dominance by certain species. When combined with manure application, reduced tillage may offer the potential for increasing diversity by reducing the competitiveness of dominant species and facilitating establishment of new or minor species. 相似文献
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KANGETSU HIRASE SATOSHI YOSHIGAI MAKOTO NISHIDA KAZUNORI TAKANAKA TATSUO SHINMI 《Weed Biology and Management》2004,4(2):71-74
MTB-951 is a potential mycoherbicide using a fungal plant pathogen ( Drechslera monoceras ) isolated from native Echinochloa species in Japan. Conidia of this pathogen were used as the active ingredient and its herbicidal performance was examined in a greenhouse. The efficacy of MTB-951 on Echinochloa crus-galli L. was higher in deep water (7–9 cm) than in relatively shallow water (3–5 cm). In a postemergence application, the efficacy decreased as the leaf stage of E. crus-galli proceeded between the 1 and 2.5 leaf stage. For example, the control ratio (%) of E. crus-galli was 95% when applied at the 1 leaf stage, and 72% at the 2.5 leaf stage in 5 cm water. Generally, mycoherbicidal efficacy was less when applied pre-emergence rather than postemergence. Efficacy was also influenced by the duration of submergence in deep water. For example, when water depth was kept at 5 cm for more than 7 days after application and then decreased down to 3 cm, the efficacy was high. However, when the water depth was kept at 5 cm for less than 7 days, the efficacy was low. Efficacy was lower under high temperatures (35°C/25°C, day/night) than under low temperatures (25°C/15°C, day/night). Water management, application timing and temperature are important factors on herbicidal efficacy of MTB-951 to control E. crus-galli . 相似文献
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ABSTRACT: Stress and immune responses were studied in Nile tilapia Oreochromis niloticus under both conditions of self-feeding and scheduled feeding. Self-feeding fish had a significantly lower cortisol level in their blood plasma than that of scheduled feeding fish. Furthermore, skin color was found to be paler in the self-feeding fish compared with that of the scheduled feeding fish. Thus, the self-feeding fish had a less stressful physiological status than the other experimental fish. Immunological analyses revealed that the self-feeding fish significantly exceeded the scheduled feeding fish in the phagocytic activity of their macrophages, antibody production, and the number of blood-circulating lymphocytes. The higher immune responses of self-feeding fish seem to be attained through a less stressful environment. This self-feeding behavior could be exploited to create reduced stress for disease control in fish farming. 相似文献