乌骨绵羊和黑色素的研究进展     

李文  邓卫东  毛华明 《家畜生态》2007,(3)

黑色素在生物体内起着重要的作用,本文从黑色素的分类、生物合成机制、分析方法、结构、理化性质和功能、分布、沉积等方面进行了阐述,并对新近在云南发现的乌骨绵羊乌质性状形成相关的黑色素的生成的基因进行了描述。  相似文献   

卵泡抑制素与GFP融合基因疫苗pEGISI的构建及表达     

曹少先  茆达干  舒邓群  程宝  王进荣  刘铁铮  杨利国 《家畜生态学报》2007,28(2):9-12

为构建卵泡抑制素与绿色荧光蛋白（GFP）融合基因疫苗pEGISI，将pcISI中的乙肝表面抗原（HBsAg）S基因及插入S基因中的抑制素（INH）基因片段酶切回收；PCR扩增pcISI中INH基因片段，调整阅读框，一起融合到pEGFP-N1中EGFP基因的5’端，构建ISI-EGFP融合表达质粒pEGISI。酶切和测序鉴定表明，重组质粒pEGISI构建成功。将pEGISI转染293T细胞，16h后检测到绿色荧光，48h检测到强烈荧光，说明融合基因在293T细胞获得高表达；ELISA检测证实，表达产物ISI—EGFP融合蛋白具有INH的抗原抗体反应原性。质粒pEGISI的成功构建及表达为抑制素基因免疫的机理及安全性研究奠定了基础。  相似文献   

肉牛补饲糖蜜舔砖的短期育肥效果研究     

王安奎  杨国荣  黄必志  陈文洋  周世春  毛华明  张存根  李建平 《中国牛业科学》2006,33(3):33-34,41

通过对内牛饲喂与不喂糖蜜舔砖的效果进行对比分析。对照组头均增重103．3g，试验组头均增重321．1g，比对照组日均增重高出217．8g，提高210％。试验组每kg增重耗用糖蜜舔砖1．38kg，对照组头均获利65．08元，试验组头均获利142．2元，比对照组高出77．12元，提高118．5％。  相似文献   

甘肃省土地资源可持续利用定量评价   总被引：1，自引：0，他引：1  

毛笑文 《干旱地区农业研究》2006,24(3):115-120

从资源、环境、经济和社会4方面构建土地资源可持续利用评价指标模型,并选择23个参评因子,对甘肃省土地资源可持续利用空间差异进行定量分析,发现甘肃省土地资源可持续利用水平有明显的空间差异,认为各地在资源开发、经济发展、社会进步和环境保护方面有待进一步协调。  相似文献   

Evaluation of Thermal Environment of Cowshed and Correlation Analysis Between Thermal Parameter and Physiological Parameter of Dairy Cows in Central Plain of Hebei     

ZHAO Shoupei  LI Xuemei  GAO Yuhong  SUN Xinsheng  GUO Jianjun  HAO Zangen  MAO Sen 《中国畜牧兽医》2007,47(11):3510-3517

The purpose of this study was to evaluate thermal environment of cowshed in four seasons in central plain of Hebei,and analyze the correlation between the temperature parameters and physiological indexes of dairy cows.Three dairy cow houses with different building structures were selected,the thermal parameters (ambient temperature (Ta) and relative humidity (RH)) and physiological parameters (respiratory rate,rectal temperature and body surface temperature) were detected.The results showed that the Ta,RH and index of temperature and humidity (THI) changed significantly in four seasons (P<0.05),the average daily temperature in summer was the highest (28.59 ℃),and the average daily temperature in winter was the lowest (1.55 ℃).In summer,dairy cows were suffering from mild heat stress for 15.5 h every day,and from moderate heat stress for 6.0 h.In winter,dairy cows were under mild cold stress for an average of 12.0 h per day.The Ta and THI of three cowsheds in each season had no significant differece (P>0.05),except for that in winter.Compared with the cowshed with low wall or roller blind,the average Ta in the cowshed with only roof was 0.80-1.27 ℃ higher in summer and 1.36-1.84 ℃ lower in winter.In addition,the physiological parameters of dairy cows were extremely significantly higher in summer than those in other seasons (P<0.01).The respiration frequency or rectal temperature of cows among cowsheds in summer were significantly different (P<0.05),and the seasonal difference in body surface temperature was significant (P<0.05).Correlation analysis of thermal parameters and cow physiological parameters showed that the respiratory frequency,rectal temperature and body surface temperature were positively correlated with THI and Ta (P<0.05).However,there was no significant correlation between physiological parameters and RH (P>0.05).The results provided scientific basis for environment evaluation of cows,and physiological status of dairy cows were inferred according to environmental thermal parameters,which provided data for occurrence and early warning of stress.  相似文献   

微卫星标记与考力代羊体重体尺性状遗传关系研究     

毛凤显  皇甫江云  赵有璋  张明忠 《畜牧与兽医》2006,38(9):15-18

本试验用单标记法研究考力代绵羊1号染色体ILSTS004~CSSM004和BM6506~MCM130之间的12个微卫星位点ILSTS004、MCMA008、MNS0094、CSSM004、BM6505、BMS4008、CSRD0298、TGLA415、BM8246、RM0509、URB0038、MCM0130,与其绵羊初生重、断奶重、体重(8月龄)、体高、体长、胸围、管围的遗传关系,结果ILSTS004位点不同基因型对初生重影响极显著(P<0·01)、对体重影响显著(P<0·05),CSSM004位点对体长影响显著(P<0·05),其余位点对体重等性状无显著影响(P>0·05)。其中,ILSTS004位点:基因型101099个体的初生重显著高于基因型095093(P<0·05),基因型101099个体的体重显著高于基因型103103(P<0·05);CSSM004基因型196196个体的体长显著大于基因型202202(P<0·05)。提示在1号染色体微卫星位点ILSTS004附近可能存在影响绵羊初生重、体重的数量性状位点(QTL),ILSTS004可作为其遗传标记之一;在CSSM004附近可能存在影响体长的QTL,CSSM004可作为其遗传标记之一。  相似文献   

Effect of nitric oxide on urotensin-Ⅱ expression in pulmonary arterioles of rats chronically exposed to hypoxia-hypercapnia     

WU Xiao-mai  FAN Xiao-fang  HUANG Hong  LUO Jian-feng  MAO Sun-zhong  HU Liang-gang  GONG Yong-sheng 《园艺学报》2006,22(10):1905-1908

AIM: To investigate the roles of nitric oxide/L-arginine (NO/L-Arg) pathway and urotensin-Ⅱ (UⅡ) in the development of pulmonary hypertension induced by chronic hypoxia-hypercapnia in rats.METHODS: Forty male Sprague-Dawley rats were randomly divided into four groups (n=10): normal control group (A), hypoxia-hypercapnia+saline group (B), hypoxia-hypercapnia+L-Arg liposome group (C) and hypoxia-hypercapnia+N-nitro-L-arginine methyl ester (L-NAME) group (D).Contents of UⅡ, UⅡ mRNA and receptor of UⅡ (UT) mRNA in pulmonary arterioles were measured with immunohistochemistry analysis and in situ hybridization, respectively.Change of small pulmonary vascular microstructure was also investigated.RESULTS: (1) The mean pulmonary artery pressure (mPAP) and the weight ratio of right ventricle to left ventricle plus septum ［RV/(LV+S)］ in B and D groups were all higher than those in A group (respectively, P<0.05), with C group significantly lower than those in B group (respectively, P<0.01).(2) Light microscopy showed that the ratio of vessel wall area to total area (WA/TA) and the media thickness of pulmonary arterioles (PAMT) in B group were higher than those in A group (P<0.05), with C group significantly lower than those in B group.(3) The contents of UⅡ, UⅡ mRNA and UT mRNA in pulmonary arterioles in B and D groups were all higher than those in A group (respectively, P<0.01), while the expression of UⅡ and UⅡ mRNA in C group were lower than those in B group (P<0.01).CONCLUSION: The pathological process of pulmonary hypertension induced by chronic hypoxia-hypercapnia might be related to upregulation of UⅡ located in pulmonary arterioles, which might be partially inhibited by exogenous NO in rats.  相似文献   

Correlation between the signal pathway IKK/NF-κB and the anti-oxidant activity in asthma rats and the modulation of Ginkgo biloba extract     

LIN Yi-ping  LI Chang-chong  HU Ye  LI Meng-rong  MAO Yu-fei  CHEN Xiao-fang 《园艺学报》2006,22(7):1388-1391

AIM:To investigate the correlation between the signal pathway of IKK/NF-κB and the anti-oxidant activity in asthmatic rats and the modulation of Ginkgo biloba extract (Egb). METHODS:Thirty-six male SD rats were randomly divided into three groups:control group (group C), asthmatic group (group A) and Egb group(group E). Asthma in rats was established by ovalbumin (OVA) challenge methods. The mRNA of IKKβ and the protein of NF-κB P65 in lung tissue were assessed by using in situ hybridization with oligonucleotide probe and immunohistochemisty, respectively. RESULTS:The expression of IKKβ mRNA and NF-κB P65 protein in group A were significantly increased when compared with group C (P＜0.01, respectively), but those data in group E were significantly decreased when compared with group A (P＜0.01, respectively). The concentrations of MDA in serum and BLAF in group A were significantly higher than those in control group (P＜0.01，respectively), but the concentrations of MDA in group E were significantly lower than those in group A (P＜0.01, respectively). The concentrations of GSH in group A were significantly lower than those in group C (P＜0.01, respectively), but the concentrations of GSH in group E were significantly higher than those in group A (P＜0.01, respectively). CONCLUSION:The expression of IKKβ mRNA and NF-κB P65 protein were increased significantly in asthmatic rats. This was possibly attributed to the decreased anti-oxidant activity. Egb increased the anti-oxidant activity and inhibited the activity of IKK/NF-κB.  相似文献   

条斑型玉米圆斑病病原鉴定及其生物学特性研究   总被引：5，自引：0，他引：5  

孙广宇  王琴  张荣  毛琦 《植物病理学报》2006,36(6):494-500

 本研究通过致病性测定、形态学和分子生物学鉴定方法,首次明确陕西省发生的玉米条斑型叶斑病是玉米圆斑病的一种症状类型,由玉米生离蠕孢(Bipolaris zeicola)3号生理小种引起。供试品种豫玉22、农大108、农大3138、东单7号、沈单10号、沈单16号、户单4号、新陕资1号、高农1号和陕单9号都对3号生理小种表现感病,品种间抗病性差异不显著。此菌在PDA、PSA、OMA、CMA、WA和Czapek上均可生长,最适生长培养基为PDA。菌落生长温度为5~35℃,最适菌落生长温度为25℃。分生孢子在5~40℃均可萌发,低于5℃高于40℃萌发率极低,萌发的最适温度为28℃。孢子在pH 3~11均可萌发,萌发最适pH为5。在自来水中分生孢子可以萌发,蔗糖和葡萄糖溶液可以明显促进孢子萌发。  相似文献   

陈皮对肉鸡生产性能及回盲肠微生物菌群的影响     

张耀  毛胜勇  朱伟云 《畜牧与兽医》2007,39(6):21-22

选用健康、体重一致的1日龄肉仔鸡450羽,随机分为5组,对照1和2为对照组,分别饲喂不添加抗生素和添加抗生素的基础日粮;A、B、C为试验组,饲喂无抗基础日粮,并分别在日粮中添加0.1%,0.2%和0.4%的陈皮,研究了陈皮对肉鸡生产性能和回盲肠乳酸菌及大肠杆菌菌落数的影响。结果表明,日粮中添加不同比例陈皮对肉鸡生产性能和回盲肠的乳酸菌及大肠杆菌菌落数产生的影响不同,这种差异在饲养的前期尤为明显,其中以添加0.2%陈皮组效果最好。  相似文献