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Porcine alpha-fetoprotein (AFP) was purified from the sera of four-day-old piglets using an immunoadsorbent column. The molecular weight of the AFP was 80,000 when determined by gel filtration on Sephadex G-200 and 75,000 when determined by sodium dodecylsulphate polyacrylamide gel electrophoresis. The isoelectric point of the AFP was pH 4.85 at 0 degree C. The maximum concentration of serum AFP was reached on the fourth day after birth (mean value 1.1 mg ml-1), and it then decreased to 10 micrograms ml-1 when the piglets were 35 days old. The concentration of serum albumin increased rapidly between birth and seven days old, reaching 16 to 17 mg ml-1 at seven days old. Between birth and seven days old, the serum concentrations of AFP and albumin were approximately inversely proportional. 相似文献
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Nikaidou S Ishizuka M Maeda Y Hara Y Kazusaka A Fujita S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(1):137-138
Green tea, one of the most popular beverages consumed in Asian countries, has been reported to possess anticarcinogenic and antimutagenic properties. The aim in this study is to test the radical scavenging effect of catechins and caffeine, which were major components of green tea, and if they really prevent oxygen radical-induced mutagenesis. We used TA102 strain of Salmonella typhimurium which is sensitive to hydroxyl radical in the Ames mutation assay. We found that caffeine did not show any effects on mutagenesis in this system, but catechin significantly reduced mutagenesis or genotoxicity caused by hydroxyl radical. This radical-scavenging action of catechins may indeed contribute to the anticarcinogenic activity of green tea as has been proposed. 相似文献
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Fujita Y Hara Y Nezu Y Yamaguchi S Schulz KS Tagawa M 《American journal of veterinary research》2005,66(12):2028-2033
OBJECTIVE: To compare activities of interleukin (IL)-1beta, IL-6, tumor necrosis factor (TNF)-alpha, and matrix metalloproteinase (MMP)-3 and contents of sulfated glycosaminoglycan (S-GAG) in joint fluid obtained from dogs with hip dysplasia (HD) and clinically normal dogs, evaluate correlations among these markers in joint fluid obtained from dogs with HD, and evaluate correlations between each marker and clinical and radiographic variables. Animals-26 dogs with HD (clinical group) and 43 clinically normal Beagles (control group). PROCEDURE: Joint fluid was aseptically collected from the hip joints of all dogs. For each dog in the clinical group, age, duration of lameness, radiographic osteoarthritis (OA) score, and Norberg angle in each affected joint were recorded. Activities of IL-1beta, IL-6, TNF-alpha, and MMP-3 and S-GAG contents were measured. Values were compared between groups by use of Mann-Whitney U tests, and the Spearman rank correlation test was used to evaluate correlations among markers and between each marker and clinical or radiographic variables. RESULTS: Values of all markers were significantly higher for the clinical group, compared with values for the control group. There was a moderate positive correlation between lameness duration and IL-6 activity and a strong negative correlation between the Norberg angle and IL-1beta activity. CONCLUSIONS AND CLINICAL RELEVANCE: Analysis of our results indicated that there was a significant increase in markers of OA in dogs with HD. Activities of IL-1beta and IL-6 in joint fluid of dogs with HD may be influenced by the severity of laxity in the hip joint and lameness duration, respectively. 相似文献
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Inamori K Yoshida-Moriguchi T Hara Y Anderson ME Yu L Campbell KP 《Science (New York, N.Y.)》2012,335(6064):93-96
Posttranslational modification of alpha-dystroglycan (α-DG) by the like-acetylglucosaminyltransferase (LARGE) is required for it to function as an extracellular matrix (ECM) receptor. Mutations in the LARGE gene have been identified in congenital muscular dystrophy patients with brain abnormalities. However, the precise function of LARGE remains unclear. Here we found that LARGE could act as a bifunctional glycosyltransferase, with both xylosyltransferase and glucuronyltransferase activities, which produced repeating units of [-3-xylose-α1,3-glucuronic acid-β1-]. This modification allowed α-DG to bind laminin-G domain-containing ECM ligands. 相似文献
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Determining the optimal rotation period was a crucial component of forest sustainable management strategies, especially under climate change. This paper had two objectives: (1) to determine the economic benefits and optimal rotation periods for timber production when coupled to carbon sequestration, as predicted by time series prediction models for Pinus tabulaeformis plantations in China; and (2) to evaluate how different carbon prices and interest rates affected optimal rotation periods using the forest land expectation value. The results suggested that time series prediction models were valuable for estimating timber volumes and carbon sequestrations based on surveys of different-aged stands. Importantly, since integrating carbon sequestrations into timber production benefits did not increase optimal rotation periods, this should promote P. tabulaeformis plantation management. In the sensitivity analysis, a higher carbon price increased the profitability of carbon sequestration and timber production, but not optimal rotation periods, though they were reduced under higher interest rates. In conclusion, incorporating both timber production and carbon sequestration benefits would sharply increase forest-based revenues, while realizing the carbon sequestration potential of P. tabulaeformis plantations. This approach was clearly useful to the development of reforestation/afforestation projects trying to mitigate climate change and also provided a theoretical basis for sustainable forest management. 相似文献
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Dey Daniel C. Knapp Benjamin O. Battaglia Mike A. Deal Robert L. Hart Justin L. O’Hara Kevin L. Schweitzer Callie J. Schuler Thomas M. 《New Forests》2019,50(1):11-40
New Forests - For millennia, natural disturbance regimes, including anthropogenic fire and hunting practices, have led to forest regeneration patterns that created a diversity of forest lands... 相似文献