The effect of electro-acupuncture stimulation on rhythm of autonomic nervous system in dogs     

Kimura Y  Hara S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(4):349-352

Effects of electro-acupuncture (EA) stimulation on the rhythm of the autonomic nervous system in dogs were studied. Six healthy beagles were used in this study. Each dog was separately kept in a cage, and repeatedly exposed to light for 12 hr and dark for 12 hr alternately. Fixed subject dogs were stimulated by use of 5-V, 250-musec, 2-Hz biphasic square pulses for 15 min at the Xuan Shu (GV-5) and Bai Hui (GV-20) points on the spine. After EA stimulation, electrocardiogram was recorded for 24 hr. From the electrocardiogram data, the heart rate (HR), coefficient of variation in the R-R intervals (CVRR; index of autonomic nervous activity), power of high frequency component (HF; index of vagal nervous activity), and ratio of powers of the low and high frequency components (LF/HF; index of sympathetic nervous activity) were obtained. Cosinor analysis demonstrated that these indices exhibited a significant rhythmicity (P<0.05), irrespective of EA stimulation. In LF/HF, EA stimulation advanced the acrophase (from 22:55 to 21:33, P=0.012), and elevated the midline-estimating statistic of rhythm (from 0.653 to 0.725, P=0.006). However, there was no significant difference in HR, CVRR, or HF. In conclusion, EA stimulation markedly influenced the rhythm of sympathetic nervous system in dogs.  相似文献   

Prevalence of antibodies to canine respiratory coronavirus in some dog populations in Japan     

Soma T  Ishii H  Miyata K  Hara M 《The Veterinary record》2008,163(12):368-369

  相似文献   

An enzyme-linked immunosorbent assay using nuclear antigen for detection of feline herpesvirus 1 antibody.     

Y Satoh  K Iizuka  M Fukuyama  S Kishikawa  Y Nishino  T Ikeda  A Kiuchi  M Hara  K Tabuchi 《Journal of veterinary diagnostic investigation》1999,11(4):334-340

To detect antibody against feline herpesvirus 1 (FHV-1) in the sera of cats, the sensitivity and specificity of an enzyme-linked immunosorbent assay (ELISA) using nuclear antigen was investigated. The standardized optical density readings (ODs) of the ELISA obtained by the 1-step serum dilution (1:80) method were compared with the serum neutralization test (SNT) results, with a correlation of 0.993, and with the hemagglutination inhibition (HI) test results, with a correlation of 0.851. The ODs for the ELISA titers were obtained using the serial serum dilution method and were compared with the SNT results, with a correlation of 0.933, and with the HI test results, with a correlation of 0.987. In the experimental infection of 4 specific-pathogen-free cats, the results of different serologic tests (SNT and HI) and the ELISA using the serial serum dilution method revealed rapid production of antibodies after inoculation, whereas the ELISA using the one-step serum dilution method indicated that titers increased more slowly. These results indicate that with the present ELISA using nuclear antigen, there are fewer demands on time and labor, making the method convenient for monitoring FHV-1 infection.  相似文献   

The effect of blood deposition on the degradation of the connective tissue of the yellowtail Seriola quinqueradiata during storage     

Jiang  Tong  Yuan  Pengxiang  Hirasaka  Katsuya  Hamada  Yuki  Hara  Kenji  Tachibana  Katsuyasu  Taniyama  Shigeto 《Fisheries Science》2019,85(6):1099-1107

Fisheries Science - Blood is deposited in fish muscle during storage, and this deposition accelerates the deterioration of flesh quality. We have examined the effect of blood deposition on the...  相似文献   

Identification of an aminopeptidase from the skeletal muscle of grass carp (<Emphasis Type="Italic">Ctenopharyngodon idellus</Emphasis>)     

Li-Gen Zhou  Bing-Xin Liu  Le-Chang Sun  Kenji Hara  Wen-Jin Su  Min-Jie Cao 《Fish physiology and biochemistry》2010,36(4):953-962

Aminopeptidases play important roles in turnover of proteins, metabolism of hormones and neurotransmission, cell maturation and immunological regulations. In the present study, an aminopeptidase was purified to homogeneity from the skeletal muscle of grass carp by ammonium sulfate fractionation and sequential chromatographic steps, including DEAE-Sephacel, Sephacryl S-200, hydroxyapatite and Phenyl-Sepharose. The purified enzyme revealed a molecular mass of approximately 105 kDa both on SDS–PAGE and on gel filtration of Superdex 200. The enzymatic activity toward synthetic substrates was optimal at 40°C and pH 7.0–7.5. Metal-chelating agents such as EDTA and EGTA effectively inhibited the enzyme activity while inhibitors to serine, asparatic and cysteine proteinases did not show much effect, suggesting its belonging to metalloproteinase family. A specific aminopeptidase inhibitor bestatin was most effective in suppressing the enzymatic activity and performed in a competitive fashion. The enzymatic activity was slightly enhanced by metal ions of Mg²⁺ and Mn²⁺ while inhibited to different extents by Co²⁺, Cu²⁺, Zn²⁺ and Ca²⁺. Sulfhydryl reagent was necessary to maintain its activity. Purified enzyme demonstrated amidolytic activity most effectively against synthetic aminopeptidase substrate Leu-methylcoumarylamide (MCA) while N-terminal-blocked substrates and myofibrillar proteins were not hydrolyzed. The enzyme purified in the present study was quite possibly a leucine aminopeptidase (LAP) and functions during muscular protein metabolism.  相似文献   

Monthly changes in the serum levels of two choriogenins and vitellogenin in male masu salmon <Emphasis Type="Italic">Oncorhynchus masou</Emphasis>     

Toshiaki Fujita  Haruhisa Fukada  Munetaka Shimizu  Naoshi Hiramatsu  Akihiko Hara 《Fisheries Science》2008,74(5):1198-1200

  相似文献   

Detection of aflatoxin B1 in imported maize kernel used as feed by enzyme-linked immunosorbent assay.     

K Hirano  Y Adachi  M Hara  S Ishibashi  M Sueyoshi  I Ueno  K Egawa  N H Kumazawa 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1991,53(4):767-768

  相似文献   

Antibody Testing Against Canine Coronavirus by Immunoperoxidase Plaque Staining     

Soma  T.  Hara  M.  Ishii  H.  Yamamoto  S. 《Veterinary research communications》2001,25(4):327-336

The application of the immunoperoxidase (IP) plaque staining procedure (IP test) to the diagnosis of canine coronavirus (CCV) infection was investigated. The IP test did not react with sera from either 15 specific pathogen-free (SPF) dogs or 7 SPF dogs immunized with a multivalent vaccine, including canine parvovirus type 2, canine distemper virus, canine adenovirus type 2, and canine parainfluenza virus. To compare the IP test with the neutralizing test (NT), sera from 240 healthy dogs and from 3 experimentally CCV-infected dogs were examined. All 60 sera positive for NT antibody were positive for IP antibody, and all 180 sera negative for NT antibody were negative for IP antibody in the healthy dogs. The IP titres showed similar changes with time after CCV inoculation to those of the NT titres in the experimentally infected dogs. These findings indicate that the IP test specifically detected anti-CCV antibodies. When the IP test and NT were compared in dogs with diarrhoeic signs. 2.1% of 48 sera and 20.3% of 74 sera, which were all negative for NT antibody, were positive for IP antibody in the dogs of under one year of age and at least one year of age, respectively. The difference between the IP and NT titres (log₁₀ [reciprocal of IP titre] – log₁₀ [reciprocal of NT titre]) for the diarrhoeic dogs of under one year of age (2.350±0.931) was significantly larger than that for the healthy dogs (0.982±0.447) (p<0.0001), the NT titre being negative or very low, despite a high IP titre in many diarrhoeic dogs. Hence, the IP test is more able to detect anti-CCV antibodies, especially in dogs showing clinical signs. The IP-positivity rate was significantly higher in the diarrhoeic dogs of under one year of age (48.7%) than in the healthy dogs (25.0%) (² = 19.844, p<0.0001), suggesting that CCV may contribute to diarrhoea in many juvenile dogs.  相似文献   

Properties of a calicivirus isolated from cats dying in an agitated state     

Sato Y  Ohe K  Fukuyama M  Furuhata K  Kishikawa S  Sakai S  Kiuchi A  Hara M  Watanabe T  Ishikawa Y  Taneno A 《The Veterinary record》2004,155(25):800-805

In June 1993, two of five pet cats kept in Yokohama city in Japan suddenly became agitated and died. Feline calicivirus (FCV) was isolated from them. One strain (FCV-S) was isolated from the spinal cord, lung and tonsil of cat 1, another (FCV-B) from the ileum, medulla oblongata and cervical spinal cord of cat 2, and a third (FCV-SAKURA) from the oral cavity of one of the three surviving cats which showed no clinical signs. These three strains were equally resistant to pH 3.0 and serologically similar to each other, but distinct from strain F9. A genetic analysis, using a 208 base pair fragment from region E of the capsid, showed that FCV-Ari had a 70.4 per cent nucleotide and 77.3 per cent amino acid homology and FCV-F9 had a 68.6 per cent nucleotide and 73.9 per cent amino acid homology with the three strains, indicating that these two strains were genetically distinct from the three new isolates. Unvaccinated cats and cats which had been vaccinated against FCV-F9 developed watery diarrhoea but did not become agitated after the administration of FCV-S. The FCV-S strain did not induce signs of excitability after it was administered orally to specific pathogen-free cats.  相似文献   

Complete cDNA sequence and mRNA expression of dog preproendothelin-3     

Fujimori Y  Uchide T  Temma K  Sasaki T  Kizaki K  Hara Y  Saida K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(10):1251-1254

The full-length cDNA of dog preproendothelin-3 (PPET3) was cloned from lung tissue using RT-PCR and rapid amplification of cDNA ends. Aside from the poly (A) tail, the full-length cDNA was 1976 bp. A polyadenylation signal sequence and one copy of a consensus sequence, ATTTA, which is related to mRNA turnover, was found in the 3' noncoding region. The cDNA had a 594-bp open reading frame encoding a 198-amino acid polypeptide. Regions corresponding to a bioactive mature ET3 peptide, an intermediate form known as big-ET3, and an ET3-like peptide were observed in dog PPET3. Expression of PPET3 mRNA was detected throughout the organs examined, which included heart, lung, liver, kidney, spleen, stomach, pancreas, duodenum, colon, uterus, ovary and testis.  相似文献