Biochemical and histological changes in the liver tissue of rainbow trout (Oncorhynchus mykiss) exposed to sub-lethal concentrations of diazinon     

Mahdi Banaee  Antoni Sureda  Ali Reza Mirvaghefi  Kamal Ahmadi 《Fish physiology and biochemistry》2013,39(3):489-501

The organophosphate insecticide diazinon is widely used to control pest in Iran. The purpose of the present study was to investigate the antioxidant and histopathological changes in the liver tissue of rainbow trout (Oncorhynchus mykiss) exposed to 0.1 and 0.2 mg/L of a commercial formula of diazinon for a period of 28 days. Antioxidant enzyme activities—catalase, superoxide dismutase, glutathione peroxidase and glutathione reductase—were determined after 7, 14 and 28 days of exposure. Histopathological analyses were performed at the 28th day. All antioxidant enzymes were induced after 7 days of diazinon treatment in both concentrations of diazinon. Catalase and superoxide dismutase maintained elevated activities during all the treatment period. Glutathione peroxidase activity returned to the control values at the 14th day, decreasing to values below control at the 28th day in both diazinon concentrations. Glutathione reductase maintained increased activities at the 14th day in the 0.1 mg/L diazinon, decreasing to control values at the 28th day. In the 0.2 mg/L group, the activity returned to control values at the 14th and decreased below the control at the 28th day. Total antioxidant capacity of hepatocytes significantly decreased in fishes exposed to diazinon during all experimental periods. Hypertrophy of hepatocytes, vacuolization of cell cytoplasm and hepatocyte cloudy swelling were observed in the liver tissue of fish exposed to both concentrations of diazinon. The results showed that diazinon altered the activity of antioxidant enzymes and the cellular total antioxidant capacity inducing oxidative stress and cellular damage in hepatocytes evidenced by histopathological analysis.  相似文献   

Predicting Cationic Exchange Capacity in Calcareous Soils of East-Azerbaijan Province,Northwest Iran     

Farrokh Asadzadeh  Mahdi Maleki-Kakelar  Farzin Shabani 《Communications in Soil Science and Plant Analysis》2019,50(9):1106-1116

The aim of this research is to study the efficiency of pedotransfer functions (PTFs) and artificial neural networks (ANNs) for cationic exchange capacity (CEC) prediction using readily available soil properties. Here, 417 soil samples were collected from the calcareous soils located in East-Azerbaijan province, northwest Iran and readily available soil properties, such as particle size distribution (PSD), organic matter (OM) and calcium carbonate equivalent (CCE), were measured. The entire 417 soil samples were divided into two groups, a training data set (83 soil samples) and test data set (334 soil samples). The performances of several published and derived PTFs and developed neural network algorithms using multilayer perceptron were compared, using a test data set. Results showed that, based on statistics of RMSE and R², PTFs and ANNs had a similar performance, and there was no significant difference in the accuracy of the model results. The result of the sensitivity analysis showed that the ANN models were very sensitive to the clay variable (due to the high variability of the clay). Finally, the models tested in this study could account for 85% of the variations in cationic exchange capacity (CEC) of soils in the studied area.

Abbreviations: ANN: arti?cial neural networks; MLP: multilayer perceptron; MLR: multiple linear regression; PTFs: Pedotransfer Functions; RBF: Radial Basis Function; MAE: mean absolute error; MSE: mean square error; CEC: cationic exchange capacity  相似文献   

Genotype by environment interaction components underlying variations in root,sugar and white sugar yield in sugar beet (<Emphasis Type="Italic">Beta vulgaris</Emphasis> L.)     

Mahdi Hassani  Bahram Heidari  Ali Dadkhodaie  Piergiorgio Stevanato 《Euphytica》2018,214(4):79

The success of plant breeding programs depends on the ability to provide farmers with genotypes with guaranteed superior performance in terms of yield across a range of environmental conditions. We evaluated 49 sugar beet genotypes in four different geographical locations in 2 years aiming to identify stable genotypes with respect to root, sugar and white sugar yields, and to determine discriminating ability of environments for genotype selection and introduce representative environments for yield comparison trials. Combinations of year and location were considered as environment. Statistical analyses including additive main effects and multiplicative interactions (AMMI), genotype main effects and genotype?×?environment interaction effects (GGE) models and AMMI stability value (ASV) were used to dissect genotype by environment interactions (GEI). Based on raw data, root, sugar and white sugar yields varied from 0.95 to 104.86, 0.15 to 20.81, and 0.09 to 18.45 t/ha across environments, respectively. Based on F-Gollob validation test, three interaction principal components (IPC) were significant for each trait in the AMMI model whereas according to F ratio (F_R) test two significant IPCs were identified for root yield and sugar yield and three for white sugar yield. For model diagnosis, the actual root mean square predictive differences (RMS PD) were estimated based upon 1000 validations and the AMMI-1 model with the smallest RMS PD was identified as the most accurate model with highest predictive accuracy for the three traits. In the GGE biplot model, the first two IPCs accounted for 60.52, 62.9 and 64.69% of the GEI variation for root yield, sugar yield and white sugar yield, respectively. According to the AMMI-1 model, two mega-environments were delineated for root yield and three for sugar yield and white sugar yield. The mega-environments identified had an evident ecological gradient from long growing season to intermediate or short growing season. Environment-focused scaling GGE biplots indicated that two locations (Ekbatan and Zarghan) were the most representative testing environments with discriminating ability for the three traits tested. Environmentally stable genotypes (i.e. G21, G28 and G29) shared common parental lines in their pedigree having resistance to some sugar beet diseases (i.e. rhizomania and cyst nematodes). The results of the AMMI model were partly in accord with the results of GGE biplot analysis with respect to mega-environment delineation and winner genotypes. The outcome of this study may assist breeders to save time and costs to identify representative and discriminating environments for root and sugar yield test trials and creates a corner stone for an accelerated genotype selection to be used in sweet-based programs.  相似文献   

Projected impacts of global warming on cropping calendar and water requirement of maize in a humid climate     

Fatemeh Karandish  Mahdi Kalanaki  Seyyed Farhad Saberali 《Archives of Agronomy and Soil Science》2017,63(1):1-13

In this research, the influence of climate change on maize cultivation was investigated and then, the possible solutions for adopting this natural hazard in the coasts of Caspian Sea in Iran was assessed. Weather data were generated for the 2011–2100 period using a statistical downscaling model under different climatic scenarios. Reference evapotranspiration (ETo) was calculated using a Neuro-fuzzy inference system. Cop water requirement was calculated by multiplying ETo by crop coefficients. Increased cardinal temperatures during 2011–2100 led to shifting in the planting date backward by 10–26 days. In addition, the projected global warming has a considerable effect on the duration of the vegetative growth stage resulting in earlier harvesting. However, the duration of the reproductive stage is less affected. Despite the obvious reduction in the length of the growing season, crop water requirement will increase by 10.6–15.3% in the future due to 1.64–28.4% increase in ETo. However, changing the cultivation time may lead to 11.2–264.5 m³ ha^?1 water saving during the whole cropping cycle through affecting both ETo and the crop growth cycle. This result demonstrates that management of the maize cropping calendar can be an effective way to achieve sustainable agriculture under future climate conditions in the study area.  相似文献   

Generation means analysis of traits related to lodging using two crosses of durum × emmer wheat     

Mohammadi  Majid  Mirlohi  Aghafakhr  Majidi  Mohammad Mahdi  Khedri  Zahra  Rezaei  Vahid 《Genetic Resources and Crop Evolution》2022,69(3):1107-1119

Genetic Resources and Crop Evolution - Lodging is one of the most important factors that affect wheat final yield. Emmer [Triticum turgidum subsp. dicoccum (Schrank ex Schübl.) Thell.] is a...  相似文献   

Effect of Glutathione-Supplemented INRA82 Extender on Miniature Caspian Stallion Sperm Quality during Storage at 5°C     

Mahdi Zhandi  Vahid Ghadimi  MSc 《Journal of Equine Veterinary Science》2014

This study was conducted to investigate the effect of glutathione-supplemented INRA82 extender on miniature Caspian stallion sperm quality during storage at 5°C. A total of 12 ejaculates from three stallions (four ejaculates from each stallion) were collected and diluted with INRA82 extender that included different concentrations of glutathione (0 [INRA-G0], 5 [INRA-G5], and 10 mM [INRA-G10]) and stored for 48 hours at 5°C. Sperm motility (computer-assisted sperm analysis), plasma membrane integrity (eosin–nigrosin staining) and functionality (hypo-osmotic swelling test), and malondialdehyde (MDA) level were determined during storage at 5°C. The results showed that the sperm total and progressive motility and plasma membrane integrity and functionality in all extenders were significantly decreased with increasing storage time. However, the MDA level in all extenders was significantly increased with increasing storage time. Also, the results showed that most of the evaluated sperm quality parameters in the present study, with the exception of MDA, were significantly greater in INRA-G5 than in INRA-G0 and INRA-G10 after 24 and 48 hours of storage at 5°C. We have concluded that supplementation of INRA82 with 5 mM glutathione can improve miniature Caspian stallion sperm quality during storage at 5°C by increasing total and progressive motility, plasma membrane integrity and functionality, and decreasing the MDA level compared with INRA-G0 and INRA-G10. More advanced in vitro evaluations and artificial insemination are required to reveal the exact effects of INRA-G5 on miniature Caspian stallion sperm quality and its fertilizing ability.  相似文献   

Root and physiological characteristics associated with drought tolerance in Iranian tall fescue     

Fatemeh Pirnajmedin  Mohammad Mahdi Majidi  Mahdi Gheysari 《Euphytica》2015,202(1):141-155

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Proteome modifications of Pacific white shrimp (Litopenaeus vannamei) muscle under biofloc system     

Akbar Abbaszadeh  Saeed Keyvanshokooh  Vahid Yavari  Mahdi Naderi 《Aquaculture Nutrition》2019,25(2):358-366

The objective of the study was to examine the effects of biofloc technology on the muscle proteome of Litopenaeus vannamei. Two biofloc treatments and one control were compared: biofloc‐based tanks under zero‐water exchange fed with 150 g/kg crude protein (BF15), or with 250 g/kg crude protein (BF25) diets, and clear water tanks with 50% of daily water exchange stocked with shrimp fed with similar amount of a 250 g/kg crude protein diet, referred to as control. The shrimp (5.28 ± 0.42 g) were divided into the 300‐L fibreglass tanks (water volume of 200 L) at a density of 35 shrimp per tank and were cultured for 35 days. The biofloc groups displayed better growth and survival compared to the control. The muscle tissue from the control and BF25 groups was subjected to proteomic analysis. Lactate dehydrogenase, enolase, arginine kinase, mitochondrial ATP synthase subunit alpha, mitochondrial ATPase inhibitor factor 1 precursor, serpin 3 and myeloid differentiation factor 88 had an increased abundance in the BF25 group, while myosin heavy chain type 1 and myosin heavy chain type 2 showed a decreased abundance. The results indicate that biofloc technology could alter the expression of proteins involved in structure, metabolism and immune status of cultured shrimp.  相似文献