Meiotic resumption of porcine immature oocytes is prevented by ooplasmic Gsalpha functions     

Morikawa M  Seki M  Kume S  Endo T  Nishimura Y  Kano K  Naito K 《The Journal of reproduction and development》2007,53(6):1151-1157

A high cyclic adenosine monophosphate (cAMP) level in fully-grown immature oocytes prevents meiotic resumption. In Xenopus, inhibitory cAMP is synthesized within oocytes depending on a stimulatory alpha-subunit of G-protein (Gsalpha). In the present study, we examined whether ooplasmic Gsalpha is involved in meiotic arrest of porcine oocytes. First, we studied the presence of Gsalpha molecules in porcine oocytes by immunoblotting, and this suggested the presence of reported isoforms (45 and 48 kDa) not only in cumulus cells but also in porcine oocytes. Then we injected an anti-Gsalpha antibody into porcine immature oocytes and found that inhibition of ooplasmic Gsalpha functions significantly promoted germinal vesicle breakdown of the oocytes, whose spontaneous meiotic resumption was prevented by 3-isobutyl-l-methylxanthine (IBMX) treatment. Although cyclin B synthesis and M-phase promoting factor (MPF) activation were largely prevented until 30 h of culture in IBMX-treated oocytes, injection of anti-Gsalpha antibody into these oocytes partially recovered cyclin B synthesis and activated MPF activity at 30 h. These results suggest that meiotic resumption of porcine oocytes is prevented by ooplasmic Gsalpha, which may stimulate cAMP synthesis within porcine oocytes, and that synthesized cAMP prevents meiotic resumption of oocytes through the signaling pathways involved in MPF activation.  相似文献   

Extracellular cyclophilin A possesses chemotaxic activity in cattle     

Satoru Takanashi  Tomonori Nochi  Miku Abe  Nanami Itaya  Megumi Urakawa  Katsuyoshi Sato  Tao Zhuang  Saori Umemura  Tomohito Hayashi  Yoshio Kiku  Haruki Kitazawa  Michael T. Rose  Kouichi Watanabe  Hisashi Aso 《Veterinary research》2015,46(1)

Cyclophilin A (CyPA) was originally discovered in bovine thymocytes as a cytosolic binding protein of the immunosuppressive drug cyclosporine A. Recent studies have revealed that in mice and humans, CyPA is secreted from cells in injured or infected tissues and plays a role in recruiting inflammatory cells in those tissues. Here we found that in cattle abundant level of extracellular CyPA was observed in tissues with inflammation. To aid in investigating the role of extracellular CyPA in cattle, we generated recombinant bovine CyPA (rbCyPA) and tested its biological activity as an inflammatory mediator. When bovine peripheral blood cells were treated with rbCyPA in vitro, we observed that rbCyPA reacts with the membranous surface of granulocytes, monocytes and lymphocytes. Chemotaxis analysis showed that the granulocytes migrate toward rbCyPA and the migration is inhibited by pre-treatment with an anti-bovine CyPA antibody. These results indicate that, as for mice and humans, extracellular CyPA possesses chemotactic activity to recruit inflammatory cells (e.g., granulocytes) in cattle, and could thus be a potential therapeutic target for the treatment of inflammation.  相似文献   

Effect of light irradiation on the antibacterial activity of compounded papers containing wasted tea leaves   总被引：1，自引：0，他引：1  

Tetsuya Takahashi  Yuji Aso  Wakako Kasai  Tetsuo Kondo 《Journal of Wood Science》2010,56(4):299-306

The endurance of the antibacterial properties of compounded papers containing wasted green tea leaves needs to be examined before considering these papers for long-term use. Hence, compounded papers containing 60 wt% wasted green tea leaves were irradiated for 1–200 h using a xenon lamp to examine the effect of irradiation on antibacterial properties of the paper. Irradiation for 20 h (cumulative ultraviolet dose at 365 nm, 6.67 × 10⁶ J/m²) or longer was found to greatly increase the antibacterial activity of the paper to a level at which no bacterial cell was confirmed to be viable. The paper was also covered with various glass filters and irradiated for 1 h. Irradiation exclusively with visible rays did not significantly affect the antibacterial activity of the paper, whereas irradiation exclusively with ultraviolet rays, even for a short time, greatly increased the antibacterial activity.  相似文献   

Molecular and biochemical characterization of a Zeta-class glutathione S-transferase of the silkmoth     

Kohji Yamamoto  Yuichi Shigeoka  Yoichi Aso  Yutaka Banno  Makoto Kimura  Takashi Nakashima 《Pesticide biochemistry and physiology》2009,94(1):30-35

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Revisiting morphological identification of Japanese jack mackerel Trachurus japonicus eggs preserved in formalin     

Masato Nishiyama  Mami Saito  Yasuhiro Sanada  Shizumasa Onoue  Akinori Takasuka  Yoshioki Oozeki 《Fisheries Science》2014,80(3):517-529

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Comparative study on general properties of alginate lyases from some marine gastropod mollusks     

Mami Hata  Yuya Kumagai  Mohammad Matiur Rahman  Satoru Chiba  Hiroyuki Tanaka  Akira Inoue  Takao Ojima 《Fisheries Science》2009,75(3):755-763

Alginate lyase (EC 4.2.2.3) is an enzyme that splits glycosyl linkages of alginate chain via β-elimination, producing unsaturated oligoalginates. This enzyme is widely distributed in herbivorous marine mollusks, brown algae, and marine and soil bacteria. In the present study, we determined the general properties and partial amino acid sequences of alginate lyases from three Archeogastropoda, i.e., Haliotis discus hannai, H. iris, and Omphalius rusticus, and one Mesogastropoda, i.e., Littorina brevicula, in order to enrich the information about functional and structural diversity in gastropod alginate lyases. The alginate lyases were extracted from hepatopancreas of these animals and purified by ammonium sulfate fractionation followed by conventional column chromatography. Single alginate lyases with molecular masses of approximately 28, 34, and 34 kDa were isolated from H. discus, H. iris, and O. rusticus, respectively. While three alginate lyases with molecular masses of 35, 32, and 28 kDa were isolated from L. brevicula. These enzymes were identified as poly(M) lyase (EC 4.2.2.3) since they preferably degraded poly(M)-rich substrate. Western blot analysis using an antiserum raised against H. discus enzyme suggested that H. iris, and O. rusticus enzymes shared similar primary/higher-order structure with H. discus enzyme, but the L. brevicula enzymes did not. H. discus, H. iris, and O. rusticus enzymes were classified to polysaccharide-lyase family-14 by the analysis of partial amino acid sequences, while the L. brevicula enzymes were not.  相似文献   

Yield improvement of Spinach (Spinacia oleracea  L.) in summer culture through use of a new kind of infrared-cutting net     

Nobuyuki Fukuoka  Kenji Murakami  Hiroki Ikoma  Sinya Morikawa  Toshiyuki Suzuki  Mami Suzuki 《The Journal of Horticultural Science and Biotechnology》2019,94(1):110-117

In this study, we examined the covering effect of a new kind of infrared ray (IR)-cutting net to improve the low yield of summer-sown spinach crops in greenhouse. This IR-cutting net gives about 61% transmittance of visible light and approx. 50–54% absorbance of IR from 700 to 2600 nm. The utilisation of IR-cutting net in summer could favour plant growth due to lowering greenhouse temperatures. The globe temperatures when shielded by the IR-cutting net were approx. 6–8ºC lower than without the net, an effect nearly equal to that of a 50% shade net. Although the plant growth was significantly reduced when plants were grown without a net, the crop productivity was intensified more by covering with the IR-cutting net than the 50% shade net. Mean fresh weight and yield for the IR-cutting net treatment was approx. 1.6 and 1.3 times larger, respectively, than for the 50% shade net. Although the photosynthetic capacity of plants grown without a net is suppressed by high temperature, this decreased photosynthetic capacity can be improved by covering with the IR-cutting net. We demonstrated that the IR-cutting net led to successful production of summer-sown spinach from the agrotechnical point of view.  相似文献   

Validation of the usefulness of 26S rDNA D1/D2, internal transcribed spacer, and intergenic spacer 1 for molecular epidemiological analysis of Macrorhabdus ornithogaster     

Atsushi KOJIMA  Nanako OSAWA  Mami OBA  Yukie KATAYAMA  Tsutomu OMATSU  Tetsuya MIZUTANI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(2):244

Macrorhabdus ornithogaster (MO) is an infectious fungus that causes gastric damage in birds. In this study, we established nested and seminested polymerase chain reaction (PCR) methods that specifically amplify the domain D1/D2 region (D1/D2) of 26S ribosomal DNA (rDNA), internal transcribed spacer (ITS) of rDNA, and intergenic spacer (IGS) 1 region from avian feces. Phylogenetic analysis of MO collected from Japanese pet birds showed little genetic variation; analysis based on these regions did not distinguish between host species order, differences in MO shape, or host gastrointestinal symptoms. These regions were found to be unsuitable for molecular epidemiological studies of MO and further investigation into other genetic regions is required.  相似文献   

Immunological characterization of peripheral blood leukocytes using vaccine for mycoplasmal pneumonia of swine (MPS) in swine line selected for resistance to MPS     

Tomoyuki Shimazu  Liushiqi Borjigin  Yuki Katayama  Meihua Li  Takumi Satoh  Kouichi Watanabe  Haruki Kitazawa  Sang‐gun Roh  Hisashi Aso  Kazuo Katoh  Yoshihito Suda  Akiko Sakuma  Mituru Nakajo  Keiichi Suzuki 《Animal Science Journal》2013,84(10):683-692

This study was conducted to evaluate immunological changes in peripheral blood leukocytes in pigs that were genetically selected for their improved resistance to mycoplasmal pneumonia of swine (MPS), using MPS vaccine as an antigen. Twelve castrated MPS‐selected Landrace pigs were compared with the same number of pigs from a nonselected line by using a time‐course analysis at the hematological level. After the second sensitization with MPS vaccine, the percentages of B cells, CD4⁺ T cells, and natural killer (NK) cells in total leukocytes were lower in the selected line than in the nonselected line, whereas the percentage of granulocytes in total leukocytes increased in the MPS‐selected line. We also assessed the proliferative ability of peripheral blood mononuclear cells (PBMCs) stimulated with Mycoplasma hyopneumoniae, lipopolysaccharide or concanavalin A, and found that although the proliferative ability of the PBMC was not different between the two lines at a steady state, the nonselected line showed a significantly higher proliferative ability after sensitization with MPS vaccine than the selected line regardless of antigens used. These results thus indicate that the selection of pigs on the basis of MPS resistance changes their immunophenotype, and would give us beneficial information for the prevention of MPS infection.  相似文献   

Investigations on the radioactive contamination of crop plants as a result of H-bomb detonation (Part 1) Radioactive contamination of crop plants and soils     

Shingo Mitsui  Sueo Aso  Kiyoshi Tensho  Kikuo Kumazawa  Kenzo Miyawaki 《Soil Science and Plant Nutrition》2013,59(1):15-16

Since radioactive rain-out covered Japan in March-May 1954, due to the H-bomb detonation at Bikini atoll, the authors started assaying the possibie radioactive contamination of crop plants grown at Tokyo district.  相似文献