全文获取类型
收费全文 | 114篇 |
免费 | 10篇 |
专业分类
林业 | 7篇 |
农学 | 1篇 |
14篇 | |
综合类 | 4篇 |
农作物 | 1篇 |
水产渔业 | 7篇 |
畜牧兽医 | 77篇 |
园艺 | 2篇 |
植物保护 | 11篇 |
出版年
2023年 | 1篇 |
2022年 | 3篇 |
2021年 | 2篇 |
2020年 | 2篇 |
2019年 | 5篇 |
2018年 | 3篇 |
2017年 | 3篇 |
2016年 | 6篇 |
2015年 | 3篇 |
2014年 | 5篇 |
2013年 | 16篇 |
2012年 | 8篇 |
2011年 | 11篇 |
2010年 | 8篇 |
2009年 | 6篇 |
2008年 | 6篇 |
2007年 | 11篇 |
2006年 | 13篇 |
2005年 | 1篇 |
2004年 | 2篇 |
2003年 | 2篇 |
2002年 | 2篇 |
2001年 | 3篇 |
2000年 | 1篇 |
1982年 | 1篇 |
排序方式: 共有124条查询结果,搜索用时 15 毫秒
31.
Hirano M Watanabe K Murakami M Kano R Yanai T Yamazoe K Fukata T Kudo T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(3):283-284
We excised surgically a feline granulomatous lesion and performed histopathological, mycological and molecular examinations. As a result, it was diagnosed as sporotrichosis, which was the second recorded case of a cat so afflicted in Japan. After the operation, we recognized another nodule on the lymph node. Histopathological examination was therefore performed, but no fungi were detected. To prevent recurrence, the cat was administered a antimycotic drug, itraconazole. As a result, no recurrence was found. Excision of the lesion is the treatment of choice for feline sporotrichosis. 相似文献
32.
Tohno M Shimosato T Moue M Aso H Watanabe K Kawai Y Yamaguchi T Saito T Kitazawa H 《Veterinary research》2006,37(6):791-812
To clarify the crucial role of Toll-like receptor (TLR) 2 and TLR9 in immature gut-associated lymphoid tissues (GALT), we focused on the expression of TLR2 and TLR9 and the immune responses induced by their ligands in the GALT of presuckling newborn swine. Quantitative real-time PCR revealed that TLR2 and TLR9 mRNA were expressed at detectable levels in all tested tissues (heart, thymus, lung, spleen, liver, kidney, skeletal muscle, duodenum, jejunum, ileum, ileal Peyer patches (Pps), and mesenteric lymph nodes (MLN)). In particular, in immature intestinal tissues and GALT, TLR2 and TLR9 mRNA were expressed at higher levels in ileal Pps and MLN than in the duodenum, jejunum, and ileum. We confirmed that the TLR2 and TLR9 proteins were also highly expressed and that their ligands were preferentially recognized by TLR2- or TLR9-expressing cells in the MLN and ileal Pps. Zymosan, CpG2006, and lactic acid bacteria could promote mitogenesis and production of multiple cytokines by the MLN and ileal Pps. In addition, double immunostaining for cytokeratin 18 and either TLR2 or TLR9 revealed that both TLR2 and TLR9 are strongly expressed in the columnar membranous (M) cells. Interestingly, while the apical membrane of the columnar M cells strongly expressed TLR2 protein and preferentially recognized zymosan, both "TLR2 expression on the apical membrane" and "TLR2-mediated zymosan binding" were negligible in neighboring enterocytes. These results indicate that TLR2 and TLR9 allow MLN and ileal Pps to respond to a variety of bacterial components immediately after birth, thereby providing newborns with a host defense system. 相似文献
33.
Michimae Y Okimoto K Toyosawa K Matsumoto I Kouchi M Tochitani T Koujitani T Funabashi H Seki T 《Journal of toxicologic pathology》2012,25(1):4141-4444
A female congenic rat produced by repeated backcrossing of Nihon rats, a model for hereditary renal cell carcinoma, to Brown Norway rats was necropsied at 24 months of age. At necropsy, a white mass about 1 centimeter in size was observed in the thoracic cavity, and the mass partly adhered to the esophagus and the diaphragm. Histologically, the mass was clearly circumscribed by connective tissue, and consisted of neoplastic cuboidal epithelial cells that showed cystic tubular proliferation. Some islands of well-differentiated hepatocytes and some vessels were observed in the mass. Immunohistochemically, the tumor cells were strongly positive for cytokeratin and partly positive for vimentin but were negative for mesothelin and Von Willebrand Factor. The positive rate for Ki-67 was 2.4%. Based on these histological and immunohistochemical evidences, we diagnosed this tumor as a cystic cholangioma that might have arisen from the ectopic hepatic tissue in the thoracic cavity. 相似文献
34.
35.
Teruo Nonomura Hiromi Tajima Yuko Kitagawa Naoko Sekiya Kayoko Shitomi Mami Tanaka Kazuhiko Maeda Yoshinori Matsuda Hideyoshi Toyoda 《Journal of General Plant Pathology》2003,69(1):45-48
Green fluorescent protein (GFP)-marked Fusarium oxysporum f. sp. melonis and nonmarked F. oxysporum f. sp. fragariae were stained with neutral red. The neutral red stained vacuoles of the fungi without disturbing GFP fluorescence in the cytoplasm.
GFP-marked fungi showed fluorescent hyphae with dark-stained vacuoles, whereas nonmarked fungi were detected as nonfluorescent
hyphae with dark-dotted vacuoles. Root colonization by these two fungi was monitored using this method. Microconidia attached
similarly to the root surface and elongated vegetative hyphae. Only the pathogenic fungi invaded, causing necrosis at the
inoculation site. Thus, the present method enabled us to track simultaneously the various formae speciales of F. oxysporum colonizing the root surface.
Received: March 25, 2002 / Accepted: September 27, 2002 相似文献
36.
Mami ADACHI Hirotaka IGARASHI Minoru OKAMOTO Takashi TAMAMOTO Yasutomo HORI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(2):296
A 10-year-old female Cavalier King Charles Spaniel presented with hematuria, pollakiuria and skin rash. Based on the histopathological and cytological examination of the skin and bladder mucosa, the dog was diagnosed with large granular lymphocytic (LGL) lymphoma of the bladder and skin. The dog responded well to the initial chemotherapy with nimustine for 3 months. Since recurrence of skin erosion and bladder wall thickening were observed, the dog was subsequently administered chemotherapy with other anticancer drugs, including chlorambucil, vincristine, doxorubicin, L-asparaginase, cytosine arabinoside, and cyclophosphamide. The dog survived for 11 months and died due to tumor-related disseminated intravascular coagulation. This is the first report of a canine case of LGL lymphoma in the skin and bladder. 相似文献
37.
The TLR Expression Pattern on Monocyte-Derived Macrophages for Lipopolysaccharid Stimulation of Calves 总被引:1,自引:0,他引:1
GUO Yi-jie ZHAO Guo-Qi HUO Yong-jiu Sachi Tana-ka Hisashi Aso Takahiro Yamaguchi 《中国农业科学(英文版)》2009,8(7):864-871
In this paper, toll-like receptor expression pattern in monocytes-derived macrophages by lipopolysaccharid (LPS) stimulation was examined. Jugular venous blood samples from 4 Japanese calves were obtained and the peripheral blood mononuclear cells (PBMC) were isolated. The PBMC were cultured for 7 d so as to collect monocytes-derived macrophages in Repcell. The PBMC were stimulated by LPS for 24 h and the mRNA expression pattern of TLR and cytokines in monocytes-derived macrophages (Mod-Mφ) was analyzed. Results showed that LPS stimulation of Mod-Mφ could increase the mRNA levels of the genes of TNF-α, IL-6, and IL-8. In addition, the mRNA levels of the genes of TNF-α and IL-6 in the group of LPS stimulation were most significantly (P 〈 0.01) higher than those in control group and the mRNA levels of TLR1, 3, 5, 8, and 10 were significantly (P 〈 0.05) decreased after LPS stimulation. There was no difference in the mRNA expressions of TLR2, 4, 6, and 7 between the groups of the control and LPS stimulation. Besides, expression of TLR9 was not found. It suggested that monocytes-derived macrophages could respond to LPS and they might take an important role in the innate immunity. The important function of the cells might contribute to better disease treatment. 相似文献
38.
Tomoyuki Shimazu Liushiqi Borjigin Yuki Katayama Meihua Li Takumi Satoh Kouichi Watanabe Haruki Kitazawa Sang‐gun Roh Hisashi Aso Katoh Kazuo Yoshihito Suda Akiko Sakuma Mituru Nakajo Keiichi Suzuki 《Animal Science Journal》2014,85(4):365-373
We recently developed a Landrace line that is resistant to mycoplasmal pneumonia of swine (MPS) infection by genetic selection for five generations, and we reported that the immunophenotype of this line is different from that of the non‐selected line in terms of changes in peripheral blood leukocyte population after MPS vaccination. This study followed up previous findings demonstrating changes in soluble factors in blood, namely, hormones, Mycoplasma hyopneumoniae‐specific immunoglobulin G (IgG), and cytokines. These two lines were injected with MPS vaccine on days ?7 and 0 after blood sampling on those days, and blood samples were collected on days ?14, ?7, 0, 2, 7 and 14. We found changes in the levels of many hormones and cytokines in both lines. However, we found that only growth hormone (GH) and interferon (IFN)‐γ levels were statistically different between these two lines. GH concentration was reduced (day 0) and IFN‐γ concentration was increased (day 14) in the MPS‐selected line compared with the non‐selected line, despite unchanged IFN‐γ messenger RNA expression in blood cells. Although detailed mechanisms underlying these phenotypes remain unsolved, these traits would be useful to improve MPS resistance in pig production and provide an insight into MPS infection. 相似文献
39.
Suda I Oki T Masuda M Nishiba Y Furuta S Matsugano K Sugita K Terahara N 《Journal of agricultural and food chemistry》2002,50(6):1672-1676
Absorption of acylated anthocyanins in purple-fleshed sweet potato (Ipomoea batatas cv. Ayamurasaki) in rats was studied to obtain evidence that the acylated anthocyanins themselves could exert a physiological function in vivo. Peonidin 3-caffeoylsophoroside-5-glucoside (Pn 3-Caf*sop-5-glc) in purple-fleshed sweet potato was directly absorbed into rat and present as an intact acylated form in plasma. After oral administration of the purple-fleshed sweet potato anthocyanin (PSA) concentrate containing 38.9 micromol of Pn 3-Caf*sop-5-glc/kg of body weight, Pn 3-Caf*sop-5-glc was detected in the plasma, and the C(max) value and t(max) were estimated as 50.0 +/- 6.8 nmol/Lof plasma and 30 min, respectively. Furthermore, the plasma antioxidant capacity was significantly elevated from 58.0 +/- 12.0 to 89.2 +/- 6.8 micromol of Trolox equivalent/L of plasma 30 min after the administration of the PSA concentrate. 相似文献
40.
Mami Harada Tadashi Tokai Michiyo Kimura Fuxiang Hu Takamichi Shimizu 《Fisheries Science》2007,73(3):477-488
ABSTRACT: In Tokyo Bay, conger tube-trap fishers voluntarily deployed escape holes of at least 13 mm diameter to reduce the by-catch of young conger smaller than the marketable size of 35 cm total length. Comparative fishing experiments in Tokyo Bay were carried out using tube traps with seven hole diameters (3, 9, 13.5, 14, 15, 17, and 19 mm), to obtain the size-selectivity of the escape-hole size for inshore hagfish Eptatretus burgeri and white-spotted conger Conger myriaster . On the assumption of the geometrical similarity in the combination of body g and hole perimeter m at a given retention probability, a single master curve s ( R ) = exp(−13.52 + 11.31 R )/(1 + exp [−13.52 + 11.31 R ]) was estimated for the two species in terms of relative R = g / m using the Share Each LEngth's Catch Total (SELECT) process based on a multinomial distribution. Almost all conger and hagfish with a girth equivalent to the hole perimeter were able to pass through the hole. Thus, the two species have a high ability to escape through a narrow space by squeezing the body through the hole in the rigid PVC tube. Optimum escape-hole size is discussed from several objectives for conger tube-trap fishery management, e.g. reduction of hagfish by-catch. This study also specifies the conditions for pooling data when fishing experiments using more than two fishing gears are replicated. 相似文献