KAv-1 is Better Suited to Chick Fibroblast Culture than DMEM or 199 Media     

Masafumi Katayama  Manabu Onuma  Tomokazu Fukuda 《The Journal of Poultry Science》2021,58(4):270

Cultured cells are a useful resource for poultry scientists, since these cells allow scientists to evaluate biological responses to conditions such as infectious diseases in vitro while mimicking the whole-body response in birds. However avian cell culture requires an optimized basal medium, and there are currently relatively few options for this basal medium (medium 199 and KAv-1). This means that there is still room for the development of an optimal basal medium for avian cell culture. Here we compare KAv-1 medium, Dulbecco''s modified Eagle medium (DMEM) and medium 199 during the culture of chick fibroblasts and determine that KAv-1 remains the optimal medium for these assays. Our results show that DNA damage is reduced in fibroblasts cultured in the KAv-1 medium, when compared to both DMEM and Medium 199 and that these cells also display improved growth dynamics in KAv-1 medium when compared to both DMEM and medium 199. To the best of our knowledge, this is the first study to describe a comparative analysis of culture media for avian cells, which would provide useful information for poultry scientists.  相似文献   

Effects of dietary Nannochloropsis sp. powder and lipids on the growth performance and fatty acid composition of larval and postlarval kuruma shrimp,Marsupenaeus japonicus     

T. O. Oswald Adissin  Ishikawa Manabu  Koshio Shunsuke  Yokoyama Saichiro  Amina S. Moss  Serge Dossou 《Aquaculture Nutrition》2020,26(1):186-200

The current study evaluated the effects of fish oil replacement with eicosapentaenoic acid‐enriched single‐cell microalgae in the diets of larval and postlarval kuruma shrimp. Experimental diets containing different level of Nannochloropsis sp. powder (10, 40, 70 g/kg) and lipids (2, 8, 10, 14, 35, 58 g/kg) were evaluated. The substitution of fish oil with algal powder significantly improved shrimp growth compared to that in the control group, with the highest final body weight recorded at 70 g/kg in larvae and 40 g/kg in postlarvae. Larvae fed algal lipids with a dry weight percentage in the diet of greater than 10 g/kg showed significantly lower performance than those in the control group. The neutral lipids in the tissue of the postlarvae that had been fed algal lipids had an improved fatty acid profile, as the content of highly unsaturated fatty acids increased compared to that in the other groups. The content of docosahexaenoic acid increased in the tissue, indicating the possible occurrence of the metabolism and accumulation of Nannochloropsis sp. essential fatty acids. Algal powder could replace FO up to 140 g/kg in shrimp diets without compromising growth and FA profiles.  相似文献   

Induction of centrum defects in amberjack Seriola dumerili by exposure of embryos to hypoxia     

Yoshifumi Sawada  Manabu Hattori  Masahide Iteya  Yasuaki Takagi  Kazuhiro Ura  Manabu Seoka  Keitaro Kato  Michio Kurata  Hirofumi Mitatake  Shigekazu Katayama  Hidemi Kumai 《Fisheries Science》2006,72(2):364-372

ABSTRACT:   Artificially hatched Seriola species have the problem of malformation, mainly in their vertebrae, head, and mouth parts. To clarify the cause of vertebral malformation, the effects of hypoxia during embryogenesis on the induction of centrum defects was investigated in artificially hatched amberjack Seriola dumerili . Firstly, 7-somite stage embryos were exposed to waters of 0, 12.5, 25, 50, and 100% dissolved oxygen (DO) for 0.5, 1, 2, 3, 4, and 5 h to confirm the effective dose (DO concentration and duration of exposure) of hypoxia that induces somitic disturbances in newly hatched larvae. Exposure of embryos to 12.5% DO concentration for longer than 0.5 h induced somitic disturbances. Following this result, centrum defects in juveniles were investigated by an induction experiment with embryos exposed to 12.5% DO for 2 h at the gastrula, 1- or 2-somite, 10-somite, 15-somite, or heart beating stage. This experiment revealed that centrum defects were induced only during somitogenesis, and somitic disturbances were the premonitory symptom of centrum defects. These results indicate hypoxia during somitogenesis as a possible cause of centrum defects in amberjack.  相似文献   

Plasma concentrations of inhibin A in cattle with follicular cysts: relationships with turnover of follicular waves and plasma levels of gonadotropins and steroid hormones     

Todoroki J  Noguchi J  Kikuchi K  Ohnuma K  Ozawa M  Kaneko H 《Domestic animal endocrinology》2004,27(4):333-344

We investigated the profiles of circulating levels of inhibin A and total inhibin in beef cows with follicular cysts in relation to the patterns of follicular development and circulating gonadotropins and steroid hormones. Turnover of follicular waves was monitored in five cows every 2 days for 70 days from 10 days after detection of estrus without ovulation. The mean interwave intervals were 19.6 ± 1.0 days (n = 18 waves with cysts from the five cows). Circulating levels of inhibin A were approximately 170 pg/ml before emergence of follicular waves with cysts and increased (P < 0.05) concomitantly with follicle emergence. High concentrations of inhibin A (greater than 300 pg/ml) were noted for 7 days during the growth phase of cystic follicles, but inhibin A levels decreased gradually when development of the cysts reached a plateau. This profile of inhibin A was similar to those of total inhibin and estradiol, but was inversely related to the changes in plasma FSH concentrations. LH pulse frequency and mean concentrations of LH in cows with cysts were higher than those observed in the luteal phase of normal cyclic cows. These results indicate that the capacity to secrete inhibin, as well as estradiol, is maintained in cystic follicles, the growth of which is extended by LH secretion at levels greater than those seen in the normal luteal phase. Inhibin A plays an important role in the extension of interwave intervals by suppressing recruitment of a new cohort of follicles.  相似文献   

Magnetic resonance imaging of neuronal ceroid lipofuscinosis in a border collie     

Koie H  Shibuya H  Sato T  Sato A  Nawa K  Nawa Y  Kitagawa M  Sakai M  Takahashi T  Yamaya Y  Yamato O  Watari T  Tokuriki M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(11):1453-1456

A castrated male border collie 23 months of age weighing 19.4 kg was referred to the Animal Medical Center of Nihon University with complaints of visual disturbance and behavioral abnormality, hyperacusis and morbid fear. The MRI examination revealed the slight dilated cerebral sulci and cerebellar fissures and left ventricular enlargement. This is the first report of MRI findings of canine neuronal ceroid lipofuscinosis.  相似文献   

Dietary Soy Peptide Enhances Thermotolerance and Survival of Juvenile Japanese Flounder,Paralichthys olivaceus          下载免费PDF全文

Janice A. Ragaza  Roger E. P. Mamauag  Saichiro Yokoyama  Manabu Ishikawa  Shunsuke Koshio 《Journal of the World Aquaculture Society》2015,46(2):129-138

Soy peptide (SP), a soy protein enzymatic hydrolysate, contains bioactive substances that could be utilized as an immune‐stimulating feed ingredient. The experiment evaluated the efficacy of dietary SP on promoting growth, and enhancing tolerance and survival to heat stress in juvenile Japanese flounder, Paralichthys olivaceus. Four diets were incorporated with different levels of SP (0, 2, 5, and 10%) and a 6‐wk feeding trial ensued. Following the feeding trial, the experimental groups were subjected to heat stress to measure survival rate and heat shock protein 70s (HSP70s) in gill, liver, and skin. Fish fed diets with SP inclusion showed considerable decrease in percent weight gain. Significantly higher lethal time values to 50% mortality (LT₅₀) value were recorded for fish fed 10% SP. Moreover, LT₅₀ values of fish fed 2 and 5% SP were significantly higher compared with fish fed control diet. HSP70s produced in all the tissues were significantly highest in fish fed 10% SP. HSP70s values were significantly higher in fish fed 2 and 5% SP compared with fish fed control diet. A significant reduction in HSP70s among all groups during recovery period was also observed. These results suggest that SP can be used to enhance the immune response and survival of P. olivaceus under heat stress.  相似文献   

Gene Expression of Growth Factors and Growth Factor Receptors for Potential Targeted Therapy of Canine Hepatocellular Carcinoma     

Gentoku IIDA  Kazushi ASANO  Mamiko SEKI  Manabu SAKAI  Kenji KUTARA  Kumiko ISHIGAKI  Yumiko KAGAWA  Orie YOSHIDA  Kenji TESHIMA  Kazuya EDAMURA  Toshihiro WATARI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(2):301-306

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Activated Vitamin D3 and Pro-activated Vitamin D3 Attenuate Induction of Permanent Changes Caused by Neonatal Estrogen Exposure in the Mouse Vagina     

Manabu MATSUDA  Keiko KUROSAKI  Naomichi OKAMURA 《The Journal of reproduction and development》2014,60(4):274-279

Exposure of mice to a high dose of estrogens including diethylstilbestrol (DES) during the neonatal period modifies the developmental plan of the genital tract, which leads to various permanent changes in physiology, morphology and gene expression. These changes include development of an abnormal vaginal epithelium lined with hyperplastic mucinous cells accompanied by Tff1 gene expression in mice. Here, the influence of vitamin D on the direct effect of estrogen on the developing mouse vagina was examined. The mid-vagina of neonatal mice was cultured in a serum-free medium containing estradiol-17β (E₂) and various concentrations of 1,25-dihydroxyvitamin D₃ (1,25(OH)₂D) ex vivo and then was transplanted under the renal capsule of ovariectomized host mice for 35 days. Exposure to E₂ alone caused the vaginal tissue to develop estrogen-independent epithelial hyperplasia and to express TFF1 mRNA, while addition of a low nanomolar amount of 1,25(OH)₂D added at the same time as E₂ to the culture medium attenuated the effects of estrogen. Expression of vitamin D receptor was also evident in the neonatal mouse vagina. Interestingly, addition of 25-hydroxyvitamin D₃, a pro-activated form of vitamin D, at the micromolar level was found to be potent in disrupting the developmental effects of E₂, while cholecalciferol was not at least at the dose examined. Correspondingly, expression of Cyp27B1, a kidney-specific 25-hydroxyvitamin D hydroxylase, was evident in the neonatal mouse vagina when examined by RT-PCR. In addition, simultaneous administration of 1,25(OH)₂D successfully attenuated DES-induced ovary-independent hyperplasia in the vagina in neonatal mice in vivo. Thus, manipulation of vitamin D influenced the harmful effects of estrogens on mouse vaginal development.  相似文献   

Transcription-coupled events associating with immunoglobulin switch region chromatin     

Nambu Y  Sugai M  Gonda H  Lee CG  Katakai T  Agata Y  Yokota Y  Shimizu A 《Science (New York, N.Y.)》2003,302(5653):2137-2140

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Dominant Factors of Major and Minor Components and Formation of Hydroxyalkanesulfonate in Urban Dew-Water     

Masaki Takeuchi  Hiroshi Okochi  Manabu Igawa 《Water, air, and soil pollution》2001,130(1-4):613-618

Dew-water was collected from January 1999 to February 2000 in Yokohama, Japan and analyzed for not only soluble major components but also insoluble or trace components. The pH of dew-water ranged from 5.36 to 6.71 and volume-weighted average pH was 5.81. In dew-water, Si, Al, Fe, Cl, and Na were abundant and they were confirmed to be transported from natural sources, sea and soil dust. The concentrations of Si, Al, Fe, and Ti contained in coarse mode aerosol were several times higher in dew-water than those in rain-water, while the concentrations of V and Pb contained in fine mode aerosol were about the same level to each other. Besides these elements derived from aerosol, the elements derived from gaseous species are also major components in dew-water. The high concentration of weak acid is one of the significant characteristics of dew-water. Bisulfite ion in dew-water is the important species as the precursor of the sulfate ion but it is stable by forming hydroxyalkanesulfonate (HASA). The HASA concentrations were evaluated by using concentrations of total S(IV) and aldehydes and hydroxymethanesulfonate (HMSA) was shown to be the dominant species in S(IV).  相似文献