St. John’s Wort Herbal Teas – Biological Potential and Chemometric Approach to Quality Control     

Kladar  Nebojša  Anačkov  Goran  Srđenović  Branislava  Gavarić  Neda  Hitl  Maja  Salaj  Nebojša  Jeremić  Katarina  Babović  Siniša  Božin  Biljana 《Plant foods for human nutrition (Dordrecht, Netherlands)》2020,75(3):390-395

Plant Foods for Human Nutrition - St. John’s wort has been used for centuries in traditional medicine of many cultures, and nowadays it is well-known as a clinically important antidepressant...  相似文献   

In vitro ruminal fermentation parameters and methane production of Marandu palisadegrass (Urochloa brizantha) in a silvopastoral system associated with levels of protein supplementation     

Alyce Raiana Monteiro dos Santos  Lívia Vieira de Barros  Matheus Lima Côrrea Abreu  Bruno C. Pedreira 《Grass and Forage Science》2020,75(3):339-350

The addition of protein supplementation in a silvopastoral system can contribute to improved forage intake and digestibility. Our objective was to evaluate in vitro ruminal parameters, digestibility and gas production of Marandu palisadegrass [Urochloa brizantha (Hochst. ex A. Rich.) R. D. Webster] in a silvopastoral system and compare this to parameters obtained from diets with protein supplementation. Forage was sampled during the growing season (November to April) in 2016/17 and 2017/18. In vitro incubation treatments consisted of four levels of protein supplement (20% of crude protein; CP) in the diet (0.1, 0.2, 0.3 and 0.4 g/kg of body weight). The neutral detergent fibre, acid detergent fibre and indigestible neutral detergent fibre concentrations were highest in the first year. In the second year, CP concentration was 21% greater than in the first year. There was a linear increase for digestion rate, a quadratic effect for lag time and a linear decrease for average digestion time as supplementation levels were increased. The least lag time and digestion time occurred in the second year. There was no supplementation effect on ruminal pH, acetate and butyrate concentrations. Second-year in vitro dry matter digestibility (IVDMD) was greater than in the first year. Increases in supplementation levels linearly enhanced IVDMD and reduced methane (CH₄) production. The inclusion of a protein supplement contributed to reduced CH₄ and increased volatile fatty acids production; therefore, we recommended the supplement inclusion of >0.28 g/kg of BW for animals grazing in well-managed palisadegrass pastures.  相似文献   

Diversity and genetic structure of the husk tomato (Physalis philadelphica Lam.) in Western Mexico     

Pilar Zamora-Tavares  Ofelia Vargas-Ponce  José Sánchez-Martínez  Dánae Cabrera-Toledo 《Genetic Resources and Crop Evolution》2015,62(1):141-153

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Genetic diversity and population structure of anciently introduced Cuban cacao Theobroma cacao plants     

Igor Bidot Martínez  Manuel Riera Nelson  Marie-Christine Flamand  Pierre Bertin 《Genetic Resources and Crop Evolution》2015,62(1):67-84

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The Humus Content and Soil Biological Properties as a Function of Organic and Mineral Fertilization     

Sándor Hoffmann  Gábor Csitári  Lívia Hegedüs 《Archives of Agronomy and Soil Science》2013,59(2):141-146

There is a growing recognition for the need to develop sensitive indicators of soil quality that reflect the effects of land management on soil and assist land users in promoting sustainability of agro-ecosystems. Three soil enzymes (dehydrogenase, phosphatase and invertase) microbial biomass as biological variables and soil organic matter content (SOM) were investigated relative to fertilization and soil fertility (estimated by crop yield) at a long-term fertilization trial (Keszthely, Hungary). 0-34.7-69.4-104.1t farmyard manure (FYM) ha m 1 5 yr m 1 and the corresponding amount of mineral fertilizers (NPK) were applied in two different crop rotation systems. There were four straw and/or stalk incorporating treatments in the second crop rotation 'B'. Enzyme activities, microbial biomass and the amount of SOM were generally higher in the fertilized soils than in the unfertilized soils. The type of amendments (mineral, FYM or mixed) had significant effects only on the amount of SOM. The correlations among the biological variables and the crop yield were generally low (r < 0.250. The differences in field management resulted only in the invertase activity.  相似文献   

Effect of hay type on cecal and fecal microbiome and fermentation parameters in horses     

Rachel J Sorensen  James S Drouillard  Teresa L Douthit  Qinghong Ran  Douglas G Marthaler  Qing Kang  Christopher I Vahl  James M Lattimer 《Journal of animal science》2021,99(1)

The effect of hay type on the microbiome of the equine gastrointestinal tract is relatively unexplored. Our objective was to characterize the cecal and fecal microbiome of mature horses consuming alfalfa or Smooth Bromegrass (brome) hay. Six cecally cannulated horses were used in a split-plot design run as a crossover in two periods. The whole plot treatment was ad libitum access to brome or alfalfa hay fed over two 21-d acclimation periods with subplots of sampling location (cecum and rectum) and sampling hour. Each acclimation period was followed by a 24-h collection period where cecal and fecal samples were collected every 3 h for analysis of pH and volatile fatty acids (VFA). Fecal and cecal samples were pooled and sent to a commercial lab (MR DNA, Shallowater, TX) for the amplification of the V4 region of the 16S rRNA gene and sequenced using Illumina HiSeq. The main effects of hay on VFA, pH, and taxonomic abundances were analyzed using the MIXED procedure of SAS 9.4 with fixed effects of hay, hour, location, period, and all possible interactions and random effect of horse. Alpha and beta diversities were analyzed using the R Dame package. Horses fed alfalfa had greater fecal than cecal pH (P ≤ 0.05), whereas horses fed brome had greater cecal than fecal pH (P ≤ 0.05). Regardless of hay type, total VFA concentrations were greater (P ≤ 0.05) in the cecum than in feces, and alfalfa resulted in greater (P ≤ 0.05) VFA concentrations than brome in both sampling locations. Alpha diversity was greater (P ≤ 0.05) in fecal compared with cecal samples. Microbial community structure within each sampling location and hay type differed from one another (P ≤ 0.05). Bacteroidetes were greater (P ≤ 0.05) in the cecum compared with the rectum, regardless of hay type. Firmicutes and Firmicutes:Bacteroidetes were greater (P ≤ 0.05) in the feces compared with cecal samples of alfalfa-fed horses. In all, fermentation parameters and bacterial abundances were impacted by hay type and sampling location in the hindgut.  相似文献   

Comparative pharmacokinetics and pharmacokinetic/pharmacodynamic analysis by nonlinear mixed‐effects modeling of cefquinome in nonpregnant,pregnant, and lactating goats after intravenous and intramuscular administration     

Nicols Javier Litterio  Augusto Matías Lorenzutti  María del Pilar Zarazaga  Martín Alejandro Himelfarb  Manuel Ignacio San Andrs‐Larrea  Juan Manuel Serrano‐Rodríguez 《Journal of veterinary pharmacology and therapeutics》2021,44(1):68-78

Cefquinome is a fourth‐generation cephalosporin that is used empirically in goats. Different physiologic factors like pregnancy or lactation could determine the pharmacokinetic behavior of drugs in the organism. The objectives of this study are to (a) compare the pharmacokinetics of cefquinome after intravenous and intramuscular administration in adult nonpregnant (n = 6), pregnant (n = 6), and lactating goats (n = 6), at a dose of 2 mg/kg, with rich sampling by nonlinear mixed‐effects modeling, (b) conduct a pharmacokinetic/pharmacodynamic analysis to evaluate the efficacy of the recommended posology in goats with different physiological states, and (c) determine the optimal posology that achieve a PTA value ≥ 90%, taking into account a T > MIC ≥ 60% of a MIC value ≤ 0.25 µg/ml, in the different subpopulations of goats for both routes. Gestation significantly increased Ka and V1, while reduced F0, Cl, and Q. On the other hand, lactation significantly increased V1 and reduced Tk0. Cefquinome concentrations achieved in placental cotyledon, amniotic fluid, and fetal serum indicate a minimal penetration across the placental barrier. Moreover, milk penetration of cefquinome was minimal. The total body clearance of cefquinome for goats was 0.29 L kg^?1 hr^?1, that is apparently higher than the reported for cows (0.13 L kg^?1 hr^?1) and pigs (0.16 L kg^?1 hr^?1). So, the optimal dose regimen for cefquinome after intravenous and intramuscular administration required higher dose and frequency of administration compared with recommendations for cows or pigs. Therefore, 2 mg kg^?1 8 hr^?1 and 5 mg kg^?1 12 hr^?1 could be used for IV and IM routes, respectively, for the treatment of respiratory infections caused by P. multocida and M. haemolytica, but only 5 mg kg^?1 12 hr^?1 by both routes should be recommended for Escherichia coli infections.  相似文献   

Swine viscera preservation in hypersaturated salt solution after alcohol fixation as a preparation method for educational purposes     

Julia Belotto Guaraná  Alois Foltran Müller  Ricardo de Francisco Strefezzi  Fabrício Singaretti Oliveira  Luciana Cristina Machado  Carlos Eduardo Ambrósio  Renata Gebara Sampaio Dória  Silvio Henrique de Freitas 《Anatomia, histologia, embryologia》2021,50(6):996-1006

The use of live animals for educational purposes is an old practice that is still employed in teaching and research institutions. However, there are several objections to this practice, whether for ethical or humanitarian reasons. Surgical techniques teaching using anatomical pieces and/or preserved cadavers promotes greater learning efficiency, provides exercise repetition and increases the confidence and satisfaction of the students when compared to the use of live animals. The current work aimed to analyse the feasibility of using fresh swine urinary bladder and small intestines (jejunum), obtained from slaughterhouses, fixed in 99.8% ethyl alcohol (EA) and preserved in sodium chloride hypersaturated solution (SCHS) at 30%, for 7, 14 and 21 days, as an alternative method for surgical skills training (SST). Swine viscera, fixed in EA and preserved in SCHS, presented a realistic appearance, absence of odour and maintained the viable morphological characteristics during the performance of the operative techniques. Preservation solutions had low cost, were easy to acquire and did not offers risks to human health. Therefore, urinary bladders and small intestines fixed in 99.8% EA for 30 days and maintained in 30% SCHS at different periods were demonstrated as a good viable option as a preservation method for surgical skills training.  相似文献   

Effects of endurance racing on horse plasma extracellular particle miRNA     

Getúlio P. de Oliveira Jr  William F. Porto  Cintia C. Palu  Lydyane M. Pereira  Alessandra M. M. Reis  Tatiana G. Marçola  Antonio R. Teixeira-Neto  Octavio L. Franco  Rinaldo W. Pereira 《Equine veterinary journal》2021,53(3):618-627

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Black soldier fly and yellow mealworm live larvae for broiler chickens: Effects on bird performance and health status     

Sara Bellezza Oddon  Ilaria Biasato  Arianna Imarisio  Miha Pipan  Dominik Dekleva  Elena Colombino  Maria Teresa Capucchio  Marco Meneguz  Stefania Bergagna  Raffaella Barbero  Marta Gariglio  Sihem Dabbou  Edoardo Fiorilla  Laura Gasco  Achille Schiavone 《Journal of animal physiology and animal nutrition》2021,105(Z1):10-18

The commercial broiler chicken strains are the result of successful selection programmes. Most of the problems related to welfare arise from the high growth rate and body weight. The use of environmental enrichments in intensive farming could have a positive effect on birds by increasing animal welfare. The aim of the study was to evaluate the effects of Hermetia illucens (HI) and Tenebrio molitor (TM) live larvae in the diets of broiler chickens on growth performance, carcass yield and health status. A total of 180 four-day-old male broiler chickens (Ross 308) were randomly allotted to 18 pens. Each pen was assigned to one of the three dietary treatments (6 replicates/treatment, 10 birds/replicate) as follows: (i) control diet (C): commercial feed (two feeding phases: starter [4–11 days] and grower [12–38 days]), (ii) HI: C + 5% of the expected daily feed intake (DFI) HI live larvae (calculated on dry matter [DM]) and (iii) TM: C + 5% of DFI TM live larvae (DM). At 39 days of age, birds were slaughtered. Growth performance parameters were overall not affected by dietary treatments, except for the grower phase feed conversion ratio (FCR) and the overall FCR being better in the TM broilers than the others (p < 0.01). No differences were observed for slaughtering performance and haematological and serum parameters, except for the spleen relative weight being higher (p < 0.01) in the birds administered with larvae when compared to the C group. Gut morphometric indexes and histopathological alterations were not influenced by insect larvae administration. In conclusion, the administration in limited quantities of HI and TM live larvae as environmental enrichment has no negative effects on broiler chicken growth performance and health status. A behavioural study could confirm that live insect larvae represent a novel natural environmental enrichment in broiler farming.  相似文献