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101.
Ribeiro Guevara Sergio Massaferro Julieta Villarosa Gustavo Arribére María Rizzo Andrea 《Water, air, and soil pollution》2002,137(1-4):21-44
Trace metals (Sb, As, Br, Cs, Co, Cr, Ag and Hg), rare earth elements (Ce, Eu, La, Lu, Sm, Tb and Yb), and Sc. were analysed in suspended load and in two sediment cores from Lake Nahuel Huapi, Nahuel Huapi National Park, Patagonia, Argentina, by using Instrumental Neutron Activation Analysis. The core activity profiles of 210Pb and 137Cs were measured to estimate the age of the sediments. Silver was enriched in the upper layers of both cores, and correlated with the strong growthof the population of Bariloche City in the last 50 yr. Concentrations are higher in the core sampled near Bariloche, andindicate some transport in the water body. Silver concentrationsmeasured in the suspended load were 4.58±0.36 and 3.46±0.40 μg g-1 in the southern margin of the lake, near San Carlos de Bariloche City, whereas in the northern margin the concentrations were 1.68±0.18 and 1.88±0.23 μg g-1. Concentration ratio for silver in suspended load and upper core layers were equal in both sampling points. These facts suggest that Ag contents correspond to inputs associated with human activities. Bromine concentrations show a strong increase abovebase line values, similar to silver. In contrast, caesium, chromium, and cobalt concentrations do not differ from base linevalues. The metalloids antimony and arsenic show little variationwith regard to base line concentration. Noticeable, the mercurycontents found in the suspended load. 相似文献
102.
Supercritical fluid extraction of all-trans-lycopene from tomato 总被引:6,自引:0,他引:6
Gómez-Prieto MS Caja MM Herraiz M Santa-María G 《Journal of agricultural and food chemistry》2003,51(1):3-7
A procedure is proposed for the supercritical fluid extraction of all-trans-lycopene from tomato using carbon dioxide at 40 degrees C without modifier. The present method minimizes the risk of degradation via isomerization and oxidation of health-promoting ingredients, such as lycopene. The effect of different experimental variables on the solvating power of the supercritical fluid was evaluated in terms of both the selectivity achievable in the process and the yield of the extraction of all-trans-lycopene. Satisfactory separations of the all-trans-lycopene isomers from the cis counterparts were achieved using a C(30) column. The obtained extract contained 88% all-trans-lycopene and 12% cis-lycopene. 相似文献
103.
The Penman–Monteith equation for reference evapotranspiration (ETo) estimation cannot be applied in many situations, because climatic records are totally or partially not available or reliable.
In these cases, empirical equations that rely on few climatic variables are necessary. Nevertheless, the uncertainty associated
with empirical model estimations is often high. Thus, the improvement of methods relying on few climatic inputs as well as
the development of emergency estimation tools that demand no local climatic records turns into a task of great relevance.
The present study describes different approaches based on multiple linear regression, simple regression and artificial neural
networks (ANNs) to deal with ETo estimation exclusively from exogenous records from secondary stations. This cross-station approach is based on a continental
characterization of the study region, which enables the selection and hierarchization of the most suitable ancillary data
supplier stations. This procedure is compared with different traditional and cross-station approaches, including methodologies
that also consider local temperature inputs. The proposed methods are also evaluated as gap infilling procedures and compared
with a simple methodology, the window averaging. The artificial neural network and the multiple linear regression approaches
present very similar performance accuracies, considerably higher than simple regression and traditional temperature-based
approaches. The proposed input combinations allow similar performance accuracies as ANN models relying on exogenous ETo records and local temperature measurements. The cross-station multiple linear regression procedure is recommended due to
its higher simplicity. 相似文献
104.
CENH3 is a centromere-specific histone H3 variant essential for kinetochore assembly. Despite its central role in centromere function, there has been no conclusive evidence supporting CENH3 as sufficient to determine centromere identity. To address this question, we artificially targeted Drosophila CENH3 (CENP-A/CID) as a CID-GFP-LacI fusion protein to stably integrated lac operator (lacO) arrays. This ectopic CID focus assembles a functional kinetochore and directs incorporation of CID molecules without the LacI-anchor, providing evidence for the self-propagation of the epigenetic mark. CID-GFP-LacI-bound extrachromosomal lacO plasmids can assemble kinetochore proteins and bind microtubules, resulting in their stable transmission for several cell generations even after eliminating CID-GFP-LacI. We conclude that CID is both necessary and sufficient to serve as an epigenetic centromere mark and nucleate heritable centromere function. 相似文献
105.
Vesicular stomatitis is an economically important arboviral disease of livestock. Viremia is absent in infected mammalian hosts, and the mechanism by which insects become infected with the causative agents, vesicular stomatitis viruses, remains unknown. Because infected and noninfected insects potentially feed on the same host in nature, infected and noninfected black flies were allowed to feed on the same host. Viremia was not detected in the host after infection by a black fly bite, but because noninfected black flies acquired the virus while co-feeding on the same host with infected black flies, it is concluded that a viremic host is not necessary for an insect to be infected with the virus. Thus co-feeding is a mechanism of infection for an insect-transmitted virus. 相似文献
106.
Marta Crivos María Rosa Martínez María Lelia Pochettino Carolina Remorini Cynthia Saenz Anahí Sy 《Agriculture and Human Values》2004,21(2-3):111-125
Through the ethnographic record of the subsistence activities partially or completely performed in the domestic sphere in two Mbyá-Guaraní settlements in Misiones (Argentina), we outline factors important in describing the local natural environment. Data was collected through systematic observation and also through semi-structured interviews. Analysis indicates that the natural environment of the area is characterized by the indigenous community in several different ways. Thus, local people view the environment as made up of different “micro-environments,” and they consequently think of the elements that compose these “micro-environments” as having different, distinct characteristics. In the context of their daily activities, both these “micro-environments” and the elements that compose them are regarded as resources, in that they are viewed in terms of what is in them relevant to the subsistence of these groups in the rainforest. It is the intention of this study to initiate the systematic recording and processing of information on how these indigenous communities know and manage the natural resources available to them in their daily life. 相似文献
107.
Pertussis toxin S1 mutant with reduced enzyme activity and a conserved protective epitope 总被引:28,自引:0,他引:28
W N Burnette W Cieplak V L Mar K T Kaljot H Sato J M Keith 《Science (New York, N.Y.)》1988,242(4875):72-74
Pertussis toxin (PTX) is a major virulence factor in whooping cough and can elicit protective antibodies. Amino acid residues 8 to 15 of PTX subunit S1 are important for the adenosine diphosphate-ribosyltransferase activity associated with the pathobiological effects of PTX. Furthermore, this region contains at least a portion of an epitope that elicits both toxin-neutralizing and protective antibody responses in mice. The gene encoding the S1 subunit was subjected to site-specific mutagenesis in this critical region. A mutant containing a single amino acid substitution (Arg9----Lys) had reduced enzymatic activity (approximately 0.02% of control) while retaining the protective epitope. This analog S1 molecule may provide the basis for a genetically detoxified PTX with potential for use as a component of an acellular vaccine against whooping cough. 相似文献
108.
Myoglobin diffusion in bovine heart muscle 总被引:15,自引:0,他引:15
The rotational mobility of myoglobin in situ was determined by proton nuclear magnetic resonance line width measurements of a characteristic myoglobin resonance observed in bovine heart muscle spectra. The protein diffuses intracellularly at nearly half the rate observed in dilute solution. This high mobility allows the oxygenated form of myoglobin to contribute significantly to the overall diffusive flux of oxygen in respiring heart muscle. 相似文献
109.
Both impulsivity and novelty-seeking have been suggested to be behavioral markers of the propensity to take addictive drugs. However, their relevance for the vulnerability to compulsively seek and take drugs, which is a hallmark feature of addiction, is unknown. We report here that, whereas high reactivity to novelty predicts the propensity to initiate cocaine self-administration, high impulsivity predicts the development of addiction-like behavior in rats, including persistent or compulsive drug-taking in the face of aversive outcomes. This study shows experimental evidence that a shift from impulsivity to compulsivity occurs during the development of addictive behavior, which provides insights into the genesis and neural mechanisms of drug addiction. 相似文献
110.