Testing of the protective effect of cell-free lyophilized vaccine against Marek's disease in field trials]     

A Kozusníková  V Benda  S Kalafa  K Pribán  J Zezulák 《Veterinární medicína》1975,20(7):355-362

The protective effect of a lyophilized vaccine against Marek's disease ("Keramvac"--Pfizer), prepared from a cell-free turkey's herpesvirus (strain FC 126), was compared in field trials with losses in the group of non-vaccinated chickens. Under the indicated conditions, the vaccine had only a 50.86% effectiveness. The possible causes of the reduced vaccination effect are discussed with regard to the pathomorphological and virological findings suggesting, among others, an increased incidence of the symptoms of the classical form of Marek's disease in the population investigated.  相似文献   

Development of puberty in the White Fulani (Bos indicus) bull calf.     

T A Aire  J U Akpokodje 《The British veterinary journal》1975,131(2):146-151

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Rapeseed Meal and egg taint     

A. Hobson‐Frohock  R. G. Fenwick  D. G. Land  R. F. Curtis  A. L. Gulliver 《British poultry science》1975,16(2):219-222

1. Certain rapeseed meals in the diet of hens laying brown eggs result in the production, from some birds, of eggs which have a “ fishy ” or “ crabby ” odour because of the presence of trimethylamine.

2. Such susceptible birds have been used to demonstrate that the activity can be extracted from rapeseed meal with appropriate solvents.  相似文献   

The role of histamine and fish meal in the incidence of gizzard erosion and pro‐ventricular abnormalities in the fowl     

E. G. Harry  J. F. Tucker  A. P. Laursen‐Jones 《British poultry science》1975,16(1):69-78

1. The role of certain fish meals in the production of localised gizzard erosion is confirmed.

2. Lesions typical of gizzard erosion could be produced by the addition of histamine to the diet.

3. The amount of histamine occurring naturally in fish meal depends on the species of fish and the extent and nature of bacterial spoilage.

4. These variables may explain why histamine has not been implicated previously and also why there have not been consistent associations between the condition and geographical source or common factors in bulk consignments of the meal.  相似文献   

Impact of environmental factors on infestation of tea leaves byHelopeltis theivora, and associated changes in flavonoid flavor components and enzyme activities     

U. Chakraborty  N. Chakraborty 《Phytoparasitica》2005,33(1):88-96

Attack of tea plant (Camellia sinensis (L.) O. Kuntze) leaves by the tea mosquito bugHelopeltis theivora (Hemiptera: Miridae) was positively correlated to temperature and rainfall, and partially to humidity, as determined in 12 varieties during the period 2000–2002. The insect attack was severe during the months of May–September, which had high temperature and rainfall, and led to severe loss of biomass due to curling and drying up of the leaves. The biochemical response of these 12 varieties of tea to attack by the insect was determined with special reference to oxidative enzymes and flavonoid flavor components. Insect attack led to an increase in the activities of the oxidative enzymes peroxidase, ascorbate peroxidase and polyphenol oxidase. Activities of phenyl alanine ammonia lyase generally decreased as a result of insect attack, which was significant in the United Planters Association of South India (UPASI) varieties. A significant decrease in polyphenols was also obtained in UPASI varieties. HPLC analysis of catechins revealed a decrease in some of the catechins in the infected leaves. Analysis of theaflavins from infusion of healthy andHelopeltis-infested tea leaves revealed no changes. http://www.phytoparasitica.org posting Dec. 19, 2004.  相似文献   

Effect of diet composition and slaughter weight on animal performance, carcass and meat quality, and fatty acid composition in veal calves     

C. Vieira  M.D. García  A.R. Mantecón 《Livestock Production Science》2005,93(3):263-275

The effect of true milk use in the diet of Spanish Brown Swiss male calves on animal performance, carcass and meat quality, and fatty acids composition was studied. In experiment 1, the effect of milk intake [ad libitum continuous (ADLIB) feed vs. restricted 0.7 during 75 days followed by ad libitum feed (RESTR)] and slaughter endpoint (225 kg vs. 5 month) were studied. In experiment 2, ad libitum concentrate feeding [grain-fed (GF)] was compared with milk supplementation until slaughter [milk-fed (MF)] in calves slaughtered at 345 kg. As regards to milk intake, carcass weight and degree of fatness were higher in the ADLIB group (P<0.05). The RESTR group revealed a higher percentage of saturated fatty acids (P<0.05). In experiment 2, the MF group exhibited a higher fat percentage (P<0.05), lower press and cooking losses (P<0.05), and higher scores for tenderness and juiciness (P<0.05) than the GF group. The percentage of saturated fatty acids was higher in the MF group (P<0.05). The results suggest that true milk use in veal production could be an advantageous alternative in terms on production costs, animal performance, and carcass and meat quality.  相似文献   

Pregnancy Rates in Repeat-breeder Heifers Following Multiple Artificial Inseminations during Spontaneous Oestrus     

Bhupender Singh  Fernando Saravia  Renée B?ge  Heriberto Rodríguez-Martínez 《Acta veterinaria Scandinavica》2005,46(1):1-12

Hormonal asynchronies during oestrus, related to the presence of suprabasal plasma-progesterone (P4) concentrations and a delayed ovulation, interfere with the fertility of repeat-breeder heifers (RBH). Since tubal dysfunction can occur in connection with hormonal asynchronies and constrained availability of fertile spermatozoa at the time of ovulation, the present study tested the hypothesis that frequent sperm deposition from onset of oestrus to ovulation may improve pregnancy rates in RBH. Five RBH and five virgin heifers (VH; controls) were repeatedly artificially inseminated (AI) at 6 h intervals from onset of oestrus to spontaneous ovulation. Hormone analyses revealed suprabasal P₄ concentrations and a delay in the occurrence of the luteinising hormone (LH) surge, but a normal cortisol profile in RBH. Compared with controls, RBH presented longer interval from onset of oestrus to ovulation, and therefore, received more AIs. Pregnancy rates in RBH reached control levels (60%; NS), indicating that the hypothesis might be correct. Pregnancy rates in VH were below the expected range, presumably attributed to a deleterious influence of the frequent handling. The study suggests that pregnancy rates can be improved in RBH by frequent AI in relation to spontaneous ovulation. However, this practice of repeated manipulations, while seeming not to show adverse effects, lacks practicality for routine use.  相似文献   

C-reactive protein measurement in canine saliva.     

María Dolores Parra  Fernando Tecles  Silvia Martínez-Subiela  José Joaquín Cerón 《Journal of veterinary diagnostic investigation》2005,17(2):139-144

An established time-resolved immunofluorometric assay designed for measurement of C-reactive protein (CRP) in canine blood was evaluated and validated for use in canine saliva. C-reactive protein was measured in saliva specimens from 5 healthy dogs before and after the injection of casein and in 37 dogs with different disease conditions. The analytical and functional limits of detection were 0.000053 microg/ml and 0.0091 microg/ml, respectively, and intra- and interassay coefficients of variation ranged between 6.7-9.9% and 8.5-16.5%, respectively. A recovery experiment showed no significant disagreement between detected values and expected ones, and saliva CRP concentration was measured in a linear and proportional manner. A positive correlation was found between CRP levels obtained in saliva and serum samples in the experimental (R2 = 0.76) and clinical studies (R2 = 0.70). The assay was able to detect significant differences between salivary CRP levels in healthy dogs and dogs with inflammatory processes. These results suggest that saliva can be used for CRP measurement in dogs. The use of saliva presents the advantage of an easier and less stressful sampling method for the animals, which might be performed outside of hospital environments.  相似文献   

Culture of Differentiated Adult Rabbit Auricular Chondrocytes     

V. Villar  B. Colao  I. Calles‐Venal  J. G. Fernndez‐lvarez  M. Fernndez‐Caso  J. M. Villar 《Anatomia, histologia, embryologia》2005,34(Z1):54-54

Chondrocytes dedifferentiate to a fibroblast‐like phenotype on plastic surfaces. Dedifferentiation is reversible if these cells are then cultured embedded in gels as alginate, agarose or collagen. Chondrocytes cultured in suspension on a non‐adherent surface are also known to form aggregates of differentiated cells. The knowledge of chondrocyte behavior in culture is relevant for tissue engineering purposes. In this report we describe a simple method to culture differentiated or redifferentiated rabbit auricular chondrocytes on plastic surfaces with a stable phenotype. When chondrocyte aggregates formed in suspension are next seeded on plastic surfaces, most of them attach to the plastic as round or polygonal cells, and this morphological differentiation, confirmed by the presence of type II collagen, is stable for long culture periods. We also report that the addition of aggregates to monolayer cultures of dedifferentiated chondrocytes results in their redifferentiation, as is shown by their morphological changes and the synthesis of type II collagen. Therefore, this simple method can be useful for the study of chondrocyte behavior on plastic surfaces and for redifferentiating previously proliferated chondrocytes in tissue engineering techniques. Furthermore, these results demonstrate that, in addition to culture conditions such as cell isolation method or cell‐density, chondrocyte behavior on plastic depends on the presence or absence of aggregates resulting from the dissociation process.  相似文献   

Serodiagnosis of Burkholderia mallei Infections in Horses: State‐of‐the‐art and Perspectives     

H. Neubauer  L. D. Sprague  R. Zacharia  H. Tomaso  S. Al Dahouk  R. Wernery  U. Wernery  H. C. Scholz 《Zoonoses and public health》2005,52(5):201-205

Burkholderia mallei causes glanders or farcy in solipeds, a disease that must be reported to the OIE (Office International des Epizooties, Paris, France). The number of reported outbreaks has increased steadily during the last decade. Serodiagnosis is hampered by the considerable number of false‐positives and ‐negatives of the internationally prescribed tests. The major problem leading to low sensitivity and specificity of complement fixation test (CFT) and enzyme‐linked immunosorbent assay (ELISA) has been linked to the test antigens currently used, i.e. crude preparations of whole cells. Future perspectives for the development and evaluation of serological test kits using well‐characterized single antigens are discussed in the light of recent molecular research on B. mallei and the closely related saprozoonotic agent B. pseudomallei.  相似文献