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61.
Four genotypes of the psittacid herpesvirus (PsHV) cause Pacheco disease in parrots. Viruses that are serologically cross-reactive to the PsHVs have also been isolated from passerine species. DNA was amplified from a herpesvirus isolated from a superb starling (Lamprotornis superbus) with PsHV-specific primers and polymerase chain reaction. A comparison of the partial sequence of the UL 16 gene from this herpesvirus with sequences from viruses of known PsHV genotypes showed that the herpesvirus from the superb starling was a PsHV genotype 1 virus. This finding expands the range of birds that are known to be susceptible to PsHV genotype 1 infections and suggests that PsHVs should be considered as a differential in passerines with herpesvirus infections. 相似文献
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Brazzein is a small, potently sweet protein. Homology modeling has been used to construct a model of the ligand-binding domain of the sweet taste receptor, and low-resolution docking has been used to identify potential modes of brazzein-receptor binding. Published brazzein mutation-taste data were then used to select one of these as the most likely brazzein-receptor binding orientation. This orientation places brazzein in contact primarily with the T1R2 subunit of the receptor, and it accounts for 21 of the 23 mutation results examined. 相似文献
64.
Mellema SC Doherr MG Wechsler B Thueer S Steiner A 《Veterinary journal (London, England : 1997)》2006,172(2):274-283
To assess short- and long-term effects of bloodless castration methods with and without local anaesthesia, behavioural and cortisol responses of lambs were used as indicators of pain and distress. Seventy lambs, aged 2-7 days, were control-handled or castrated by Burdizzo or rubber ring methods with and without local anaesthesia. Either 5 mL of diluted lidocaine (4 mg/kg) or physiological sodium chloride solution was distributed in both spermatic cords and the scrotal neck. The serum cortisol response was monitored for 48 h, and behavioural and clinical traits were followed for three months. Local anaesthesia tended to reduce behavioural and cortisol responses after Burdizzo castration and provided a significant reduction after rubber ring castration. Prolonged pain after rubber ring castration with anaesthesia was not evident. If combined with local anaesthesia, both the rubber ring and the Burdizzo methods are acceptable methods for castration of lambs up to one week of age. 相似文献
65.
Tomek A Cizinauskas S Doherr M Gandini G Jaggy A 《Journal of Feline Medicine and Surgery》2006,8(4):243-253
The purpose of this study was to analyse retrospectively a feline population with intracranial neoplastic diseases, to document seizure patterns in these animals and to determine whether partial seizures were more frequently associated with structural brain lesions then generalised seizures. In addition, a comparison was made within the population with intracranial neoplasia between two groups of cats: one with and one without seizures. Special emphasis was given to the evaluation of tumour type, localisation and size of the lesion and its correlation with seizure prevalence. Sixty-one cats with histopathological diagnosis of intracranial tumour were identified. Fourteen cats (23%; group A) had a history of seizure(s). Forty-seven cats (77%; group B) had no history of seizure(s). Generalised tonic-clonic seizures were seen in eight cats (57%) and were the most common seizure pattern in our cats with intracranial neoplasia. Clusters of seizures were observed in six cats. Status epilepticus was observed in one patient. The mean age of the cats was 7.9 years within group A (median 8.5) and 9.3 years (median 10) within group B. The cats with lymphoma within both groups were significantly younger than cats with meningioma. In both groups meningioma and lymphoma were confirmed to be the most frequent tumour type, followed by glial cell tumours. The prevalence of the seizures in patients with glial cell tumours was 26.7%, 26.3% in patients with lymphomas and 15% in cases with meningiomas. In 33 cases (54.1%) the tumours were localised in the forebrain, 15 tumours (24.6%) were in the brainstem, four (6.6%) in the cerebellum and nine tumours (14.7%) had multifocal localisation. Parietal lobe and basal ganglia mostly affected group A. In group B tumours were most frequently located in the parietal and frontal lobes as well as in the diencephalon. A positive association was documented between the localisation of a tumour in the forebrain and seizure occurrence. 相似文献
66.
Rapamycin-induced insulin resistance is mediated by mTORC2 loss and uncoupled from longevity 总被引:2,自引:0,他引:2
Lamming DW Ye L Katajisto P Goncalves MD Saitoh M Stevens DM Davis JG Salmon AB Richardson A Ahima RS Guertin DA Sabatini DM Baur JA 《Science (New York, N.Y.)》2012,335(6076):1638-1643
Rapamycin, an inhibitor of mechanistic target of rapamycin complex 1 (mTORC1), extends the life spans of yeast, flies, and mice. Calorie restriction, which increases life span and insulin sensitivity, is proposed to function by inhibition of mTORC1, yet paradoxically, chronic administration of rapamycin substantially impairs glucose tolerance and insulin action. We demonstrate that rapamycin disrupted a second mTOR complex, mTORC2, in vivo and that mTORC2 was required for the insulin-mediated suppression of hepatic gluconeogenesis. Further, decreased mTORC1 signaling was sufficient to extend life span independently from changes in glucose homeostasis, as female mice heterozygous for both mTOR and mLST8 exhibited decreased mTORC1 activity and extended life span but had normal glucose tolerance and insulin sensitivity. Thus, mTORC2 disruption is an important mediator of the effects of rapamycin in vivo. 相似文献
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69.
Di Palma S Brunetti B Doherr MG Forster U Hilbe M Zurbriggen A Vandevelde M Oevermann A 《Comparative immunology, microbiology and infectious diseases》2012,35(5):429-441
This study examined the spatiotemporal immune response in listeric rhombencephalitis of ruminants in situ. Our data support the view that astrocytes facilitate the containment of infectious lesions. Results on the natural disease recapitulate observations in experimental rodent models and suggest that the mounted adaptive lymphocytic response of ruminants is effective in eliminating Listeria monocytogenes (LM). However, our data indicate earlier participation of the adaptive immune response, a stronger B lymphocyte contribution and a more protracted macrophage infiltration in the natural disease than it has been deduced from experimental models. Therefore, such models should be complemented by studies in natural host systems. Various macrophage and microglia subsets are involved in listeric rhombencephalitis and their differential contribution may account for species differences in clinical course and outcome of infection as might species differences in the B-cell response. Future functional ex vivo and in vitro studies are necessary to further investigate the findings obtained in the present study. 相似文献
70.
Protecting sheep against early season myiasis (strike) is a significant problem in northern temperate farming systems, where fully-fleeced ewes and early-season lambs approaching market weight, are at high initial risk. Here, the prevention of early-season blowfly strike in ewes was examined on matched commercial sheep farms in south west England, using a new formulation of dicyclanil (CLiKZiN(?) pour on) with a short withdrawal period of 7 days. The ewes on seven farms received a prophylactic treatment with dicyclanil (CLiKZiN 1.25% pour-on suspension) in April 2011; ewes on three control farms received no treatment. The lambs at all farms remained untreated. No adverse reactions to treatment were recorded. On the treatment farms no ewe strikes were recorded between April and June (10 weeks). On control farms, there were 16 strike cases in ewes over the same period, giving a cumulative incidence of 31 cases per 1000 ewes. On the treatment farms only one lamb was struck whereas at the control farms 10 lamb strikes were recorded. The data demonstrate that CLiKZiN was highly effective at protecting ewes against early season strike-challenge. 相似文献