A note on the structure and iron‐binding properties of egg‐shell membranes     

H. S. Tranter  N. H. C. Sparks  R. G. Board 《British poultry science》1983,24(1):123-130

1. When a solution of ferric ammonium sulphate was added to shell membranes of the domestic fowl, iron infiltrated the mantle (cortex) surrounding the cores of the individual fibres of the membranes.

2. Contraction of warm eggs in ice‐cold colloidal iron caused flooding of pore canals and contamination of the underlying shell membranes with this element.

3. Appreciable contamination of the inner shell membrane with iron persisted for 25 d in infertile eggs stored at 37.5 °C.  相似文献   

Depression of polymorphonuclear leukocyte function associated with experimentally induced Escherichia coli mastitis in sows     

J L?fstedt  J A Roth  R F Ross  W C Wagner 《American journal of veterinary research》1983,44(7):1224-1228

In a study of susceptibilities of sows from 2 herds to experimentally induced Escherichia coli mastitis, a marked difference was seen. The "susceptible" sows were from a conventional herd and "resistant" sows were from a specific-pathogen-free herd. The purpose of the study was to determine whether deficient neutrophil function was associated with increased susceptibility to E coli-induced mastitis. Four in vitro procedures were used to evaluate polymorphonuclear leukocyte (PMN) function: (i) random migration under agarose, (ii) ingestion of 125I-iododeoxyuridine-labeled Staphylococcus aureus, (iii) quantitative nitroblue tetrazolium reduction, and (iv) iodination. After parturition and intramammary inoculation with E coli, sows from the susceptible herd were neutropenic and the neutrophils which were present in the peripheral blood had reduced function. Specifically, there were depressed random migration under agarose, S aureus ingestion, and iodination when compared with PMN function in resistant sows. These data indicate that susceptibility to E coli mastitis was associated with deficiencies in PMN numbers and function. Potential causes of the neutrophil dysfunction are discussed and include possible systemic hormonal aberrations or the presence of an inapparent viral or bacterial infection.  相似文献   

A 31P topical magnetic resonance study of embryonic development in hens' eggs     

P. S. Belton  R. E. Gordon  J. M. Jones  D. Shaw 《British poultry science》1983,24(3):429-433

1. A ³¹P topical magnetic resonance study of whole chicken's eggs in vivo is described.

2. Resonances from adenosine triphosphate, phosphocreatine, inorganic phosphate and phosphorous storage protein were observed. These changed in relative intensity as the embryo developed.

3. No ill effects due to exposure to radiofrequency radiation or magnetic fields were observed.

4. It is concluded that topical magnetic resonance can make a useful contribution to the study of embryonic development in avian eggs.  相似文献   

A note on changes in egg‐shell porosity with flock age and season during the first breeding cycle of domestic ducks     

S. G. Tullett  Sheila Smith 《British poultry science》1983,24(4):501-509

1. — Changes in egg weight and egg‐shell porosity with flock age and season are presented for domestic duck flocks in their first laying cycle.

2. — The first eggs laid are of low weight and shell porosity but generally by the fifth week of lay both characteristics have increased to the values found in the mature flock.

3. —For mature flocks porosity decreases in the summer and the consequences of this are considered in relation to the observed decrease in hatch‐ability at this time.  相似文献   

Effects of lauryl sulphate on egg‐shell quality in laying hens     

V. D. Purohit  I. C. Peoples 《British poultry science》1983,24(4):517-520

1. The effect of feeding the detergent, sodium lauryl sulphate at concentrations up to 10 g/kg diet on egg‐shell quality was examined in laying hens at 24, 36 and 48 weeks of age.

2. Lauryl sulphate at 2.5 g/kg or more in the diet significantly increased shell breaking strength, shell thickness and shell weight at 24 and 36 weeks. In 48‐week‐old hens only shell breaking strength was increased significantly. Egg production and egg weight were not affected significantly by lauryl sulphate.

3. Lauryl sulphate might increase these measures of quality by increasing shell deposition and/or shell elasticity.  相似文献   

Reproductive efficiency of heifers in the new guinea highlands     

R. C. Couchman 《Tropical animal health and production》1983,15(2):69-75

Summary Bos indicus × Bos taurus heifers were exposed to bulls at four different liveweights. The age and weight at first and second conception and first calf growth rates were studied to examine the effects of uncontrolled mating prior to or at a target mating weight of 295 kg. Initial conception rate was not affected although lighter conception weights were recorded for three groups of heifers that were served when below the target weight. However, at subsequent calving only the two heavier groups calved within the 36-month trial period. The results indicated a seasonal effect on conception, 94% of the heifers conceiving during the wet season. The effect of season also influenced post-partum body weight loss, calf growth rate to weaning and subsequent conception. It is therefore an advantage to have heifers conceiving during the latter part of the wet season so that they calve early in the following wet season, provided a minimum target weight of 285 kg is reached prior to initial conception.

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Eficiencia Reproductiva De Terneras En La Sierra De Nueva Guinea

Resumen Se cruzaron ternerasBos indicus ×Bos taurus de diferentes pesos con toros reproductores. Se estudiáron así los pesos y edades a la primera y segunda parición y las tasas de crecimiento de los recién nacidos, para medir los efectos de cruzamientos incontrolados versus controlados al alcanzar las hembras un peso de 295 kg. La tasa de concepción inicial no se afectó, aunque los recién nacidos de los grupos de hembras servidas cuando tenían pesos inferiores a 295 kg, pesaron menos. Sinembargo en pariciones sucesivas, solamente las hembras servidas con pesos de 295 kg, parieron en el lapso de 36 meses que duró el experimento. Los resultados indicaron también, que la estación del año afectó las pariciones, el 94% de las hembras concibieron durante la época lluviosa. La época del año también afectó el peso posparto de los recién nacidos, la tasa de crecimiento hasta el destete y las concepciones posteriores. Es por lo tanto ventajoso cruzar las hembras durante el final de la estación lluviosa para que paran en la siguiente estación lluviosa, procurando que éstas alcancen un peso mínimo de 285 kg, antes del apareamiento.

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Efficacite Reproductrice Des Genisses Des Hautes Terres De Nouvelle Guinee

Résumé Des génisses métissesBos indicus ×Bos taurus ont été mises en présence de taureaux à 4 poids vifs différents. L'age et le poids à la première et la seconde conception ainsi que les taux de croissance du premier veau ont été étudiés pour déterminer les effets l'accouplement incontrôlé avant ou à un poids cible d'accouplement de 295 kg. Le taux initial de conception n'a pas été affecté bien que des poids plus faibles à la conception aient été observés chez trois groupes de génisses qui étaient en dessous du poids cible au moment de la saillie. Cependant, lors du vêlàge suivant, seuls les deux groupes les plus lourds ont mis bas pendant la période d'observation de 36 mois. Les résultats montrent que la saison a une influence sur la conception. 94 p. 100 des génisses conçoivent pendant la saison des pluies. La saison a aussi influencé la perte de poids après la mise-bas, le taux de croissance des veaux jusqu'au sevrage et la conception suivante. Il est donc avantageux de faire féconder les génisses pendant la seconde moitié de la saison des pluies afin qu'elles mettent bas au début de la saison des pluies suivante pourvu qu'un poids cible minimum de 285 kg soit atteint avant la fécondation.

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Equine herpesvirus abortion in Australia 1977 to 1982   总被引：1，自引：0，他引：1  

M Sabine  C Feilen  L Herbert  R F Jones  S W Lomas  D N Love  J Wild 《Equine veterinary journal》1983,15(4):366-370

Until 1977 no case of abortion caused by equine herpesvirus 1 (EHV1) had been recorded in Australia although the virus, called equine rhinopneumonitis virus, had been known to have been present at least since 1962. Outbreaks of EHV1 abortion occurred in New South Wales in 1977 and in 1981. Sporadic cases of EHV1 abortion had been confirmed in some parts of Australia each year since 1975. It was concluded that an abortigenic subtype of EHV1 had been introduced to Australia in 1977 and that the previously endemic respiratory subtype occasionally caused abortion. Virus isolation in a variety of cell cultures and histopathological examination of tissue were shown to be satisfactory methods of diagnosis of EHV1 abortion. Lung proved to be the specimen of choice. Slight serological differences between "abortigenic" and "respiratory" subtypes of EHV1 were found in cross neutralisation tests. A serological survey of 219 Sydney horses of various ages revealed that most yearlings had already acquired neutralising antibody to both subtypes.  相似文献   

Further characterization of dandelion yellow mosaic virus from lettuce and dandelion     

L. Bos  N. Huijberts  H. Huttinga  D. Z. Maat 《European journal of plant pathology / European Foundation for Plant Pathology》1983,89(5):207-222

A damaging virus isolated in the Netherlands from lettuce was studied and compared with a virus isolated from dandelion orginating from Czechoslovakia. It was found to biologically resemble dandelion yellow mosaic virus incompletely described from dandelion and lettuce in Great Britain (Kassanis, 1944, 1947) and from dandelion in Germany (Hein, 1963). Mechanical transmission was greatly improved by buffer solution and transmission byMyzus persicae seemed to be in the non-persistent manner. Longevity in vitro of the virus hardly exceeded one day. Thermal inactivation was between 60 and 65 °C and the dilution end-point was between 10 000 and 100 000. It was still infectious in leaf material dried and stored over CaCl₂ at 4 °C for 6 1/2 years. The virus was isolated and purified with difficulty and was found to consist of one type of spherical particle of ca 30 nm diameter, with a sedimentation coefficient of 159 S, a buoyant density of 1.42 g.cm^?3 and an A₂₆₀/A₂₈₀ ratio of 1.67. An antiserum was prepared with a titre of 256 in the agar double-diffusion test. The virus could be identified in crude extracts from lettuce andChenopodium amaranticolor by enzyme-linked immunosorbent assay (ELISA), but not by agar double diffusion. It could only be visualized in crude sap in the electron microscope after trapping of virus particles on antiserum-coated grids. The virus cannot yet be assigned to any known virus group. It is of potential economic importance to lettuce because of its occurrence in widely differing regions in Europe, its aggressiveness and virulence on 22 out of 23 lettuce cultivars tested (and on endive) and its pathogenicity toLactuca genotypes which are resistant to lettuce mosaic virus and other important pathogens of lettuce. ‘Laibacher Eis’ was the only cultivar showing some tolerance.  相似文献   

Synthetic pyrethroids and avermectin for controlling the grapevine pestsLobesia botrana,Cryptoblabes gnidiella andDrosophila melanogaster     

I. Ishaaya  E. Gurevitz  K. R. S. Ascher 《Phytoparasitica》1983,11(3-4):161-166

Field treatments in a vineyard with 0.015 or 0.01% a.i. of cypermethrin, fenvalerate, fenpropathrin or AC-222,705 were more efficient in controlling the grape-berry moth (Lobesia botrana Schiff.) and the honeydew moth (Cryptoblabes gnidiella Mill.) than four standard treatments consisting of two with 0.05% a.i. fenitrothion and two with 0.075% a.i. diazinon. In pyrethroid-treated plots, infestation at the end of the trials ranged between 2.5 and 12%, compared with 21% in the standard treatment plots and 34% in the untreated plots. Cypermethrin, fenpropathrin and AC-222,705 exhibited similar field activity, while that of fenvalerate was somewhat lower. Under laboratory conditions, cypermethrin at 0.005 and 0.01% a.i. was significantly more potent than fenvalerate, fenpropathrin and AC-222,705; at a higher concentration, 0.015% a.i., all pyrethroids were highly active, with mortality ranging between 75 and 95%. Under laboratory conditions the vinegar fly (Drosophila melanogaster Meig.) was in general more susceptible to pyrethroids than was the grape-berry moth. Cypermethrin and AC-222,705 at 0.005% a.i. and avermectin at 0.0035% a.i. were potent compounds against the vinegar fly and more active than fenvalerate and fenpropathrin.  相似文献   

Biochemical properties of the pathogenesis-related proteins from tobacco     

J. F. Antoniw  R. F. White 《European journal of plant pathology / European Foundation for Plant Pathology》1983,89(6):255-264

This review describes the discovery and identification of the pathogenesis-related proteins (PRs) from tobacco. In crude leaf extracts the PRs are distinguished from the proteins in uninfected plants by their solubility at pH 3, resistance to a range of proteases, and mobility in polyacrylamide gels upon electrophoresis (PAGE) in non-denaturing conditions. PAGE has been used as a qualitative and semi-quantitative assay for PRs, and their migration in gels made from different acrylamide concentrations has been used to identify charge and size isomers and electrophoretically identical PRs in different tobacco cultivars. The subunit composition and molecular weight (mol. wt) of the four PRs identified first in Xanthi-nc were determined by SDS-PAGE; staining the gels has shown that these same four proteins in Samsun NN did not contain carbohydrate, lipid or nucleic acid, nor were they isozymic forms of twenty five enzymes known to increase in activity following infection with TMV. Evidence suggests that most of the PRs in Xanthi-nc and Samsun NN are extracellular.The purification of several PRs from Xanthi-nc, Samsun NN and other tobaccos is described, as well as their mol. wt, subunit and amino acid composition. PRs 1a, b and c consist of a single polypeptide and have similar mol. wt and amino acid compositions. Antisera prepared against purified Xanthi-nc b₁ protein have been used to determine serological relationships between PRs and form the basis of a very sensitive quantitative assay using ELISA. The regulation of synthesis of some PRs has been shown to involve translational control.  相似文献