Clonality and phenotyping of canine lymphomas before chemotherapy and during remission using polymerase chain reaction (PCR) on lymph node cytologic smears and peripheral blood     

Dilini N. Thilakaratne  Monique N. Mayer  Valerie S. MacDonald  Marion L. Jackson  Brenda R. Trask  Beverly A. Kidney 《The Canadian veterinary journal. La revue veterinaire canadienne》2010,51(1):79-84

Polymerase chain reaction (PCR) assays for the immunoglobulin and T-cell receptor genes were utilized to determine phenotype and clonality from lymph node cytologic smears and peripheral blood lymphocytes from 10 dogs with lymphoma, before chemotherapy and during remission. Results were compared with those from 13 dogs with a cytologic diagnosis of lymph node hyperplasia. Clonality was identified in 7 of the lymphomas on the basis of either lymph node cytology or peripheral blood lymphocytes before treatment. No lymph node hyperplasia samples were clonal. In 6 of the dogs with lymphoma, clonality was demonstrated during clinical remission. Detection of PCR clonality during clinical remission is an effective means of identifying minimal residual disease in canine lymphoma and thus additional work is warranted to determine if molecular remission is prognostic or predictive for outcome in well-controlled and well-defined lymphoma subtypes.  相似文献   

Influence of seed- borne and soil- carried inocula of Dickeya spp. on potato plant transpiration and symptom expression     

Marion Ansermet  Santiago Schaerer  Isabelle Kellenberger  Maud Tallant  Brice Dupuis 《European journal of plant pathology / European Foundation for Plant Pathology》2016,145(2):459-467

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Fatty acid utilization during the early larval stages of Florida pompano (Trachinotus carolinus) and Common snook (Centropomus undecimalis)          下载免费PDF全文

Marion R Hauville  Kevan L Main  Hervé Migaud  J. Gordon Bell 《Aquaculture Research》2016,47(5):1443-1458

The pattern of conservation and loss of fatty acids from the yolk sac during the endogenous feeding period and subsequent starvation was studied in pompano and snook larvae. Fundamental information on the early fatty acid dynamic and mobilization of pompano and snook larvae was collected. In both species, fatty acids were utilized as an energy source after hatching. Mono‐unsaturated fatty acids were catabolized, while saturated and poly‐unsaturated fatty acids were conserved. High levels of arachidonic acid (ARA) in pompano and snook eggs, as well as selective retention in the unfed larvae suggest a high dietary requirement for this fatty acid during the early stages of larval development. The effect of an ARA supplementation was therefore investigated in snook larvae at the rotifer feeding stage. The fatty acid profile of the larvae was successfully influenced to match that of wild eggs; however, no significant improvement in growth or survival was observed. Future research should be carried out over a longer period of time and include factors related to stress resistance.  相似文献   

Isolation,partial identification and application of diazotrophic rhizobacteria from traditional Indian rice cultivars     

Bhavanath Jha  Mukund C. Thakur  Iti Gontia  Valerie Albrecht  Marion Stoffels  Michael Schmid  Anton Hartmann 《European Journal of Soil Biology》2009,45(1):62-72

A diversity of N₂-fixing (diazotrophic) bacteria was isolated from two traditional rice cultivars, Sataria and Kartiki, from the rice growing area of Mithila region of North Bihar, India, where low levels of nitrogen fertilizers are applied. Nitrogen-free semisolid media NFb, JMV and LGI with different carbon sources and pH-values were used for enrichment and isolation of root-associated diazotrophs. The colonization density of roots by diazotrophs, as estimated from positive pellicle formation at highest dilution in nitrogen-free enrichment media, was 10⁶–10⁸ diazotrophic bacteria per g fresh root weight. Roots of the cultivar Kartiki were found to be more densely colonized endophytically by diazotrophs as detected after chloramine T (1%) surface disinfection. To ascertain the phylogenetic affiliation of the isolates, phylogenetic oligonucleotide probes and the Fluorescent in situ Hybridization (FISH) technique were applied. Using group-specific rRNA directed oligonucleotide probes, the majority of the isolates could be identified as alpha-, beta-, or gamma-proteobacteria. Using 16S and 23S rRNA-directed genus- or species-specific probes, Herbaspirillum seropedicae, Azospirillum amazonense, Burkholderia cepacia/vietnamiensis, Rhizobia and Pseudomonas spp. were found to be the most prominent root associated culturable diazotrophs. Diazotrophic Gluconacetobacter spp. were also demonstrated as colonizers of rice roots. Burkholderia cenocepacia, Pseudomonas sp. and three diazotrophic PGPR reference strains were used for the inoculation of axenically grown rice seedlings to determine the plant growth promoting potential. Significant increases in the shoot length (up to 60%), shoot dry weight (up to 33%) and the grain yield (up to 26%) per plant were observed in non-axenic pot and field trials. Using semisolid enrichment media after surface sterilization of field grown inoculated rice roots and oligonucleotide probing of the diazotrophic enrichment cultures, a sustainable colonization with the inoculated bacteria could be demonstrated.  相似文献   

THE EFFECT OF CHOPPING, LACERATING AND WILTING OF HERBAGE ON THE CHEMICAL COMPOSITION OF SILAGE     

J. C. Murdoch    D. A. Balch    Muriel C.  Holdsworth Marion  Wood 《Grass and Forage Science》1955,10(2):181-188

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Risk based culling for highly infectious diseases of livestock     

Te Beest DE  Hagenaars TJ  Stegeman JA  Koopmans MP  van Boven M 《Veterinary research》2011,42(1):81

ABSTRACT: The control of highly infectious diseases of livestock such as classical swine fever, foot-and-mouth disease, and avian influenza is fraught with ethical, economic, and public health dilemmas. Attempts to control outbreaks of these pathogens rely on massive culling of infected farms, and farms deemed to be at risk of infection. Conventional approaches usually involve the preventive culling of all farms within a certain radius of an infected farm. Here we propose a novel culling strategy that is based on the idea that farms that have the highest expected number of secondary infections should be culled first. We show that, in comparison with conventional approaches (ring culling), our new method of risk based culling can reduce the total number of farms that need to be culled, the number of culled infected farms (and thus the expected number of human infections in case of a zoonosis), and the duration of the epidemic. Our novel risk based culling strategy requires three pieces of information, viz. the location of all farms in the area at risk, the moments when infected farms are detected, and an estimate of the distance-dependent probability of transmission.  相似文献   

Analysis of bacterial load and prevalence of mixed infections with Lawsonia intracellularis, Brachyspira hyodysenteriae and/or Brachyspira pilosicoli in German pigs with diarrhoea     

Reiner G  Hillen S  von Berg S  Kixmöller M  Willems H 《Berliner und Münchener tier?rztliche Wochenschrift》2011,124(5-6):236-241

Lawsonia (L.) intracellularis, Brachyspira (B.) hyodysenteriae and B. pilosicoli are important pathogens in domestic pig production world-wide, responsible for porcine intestinal adenomatosis, swine dysentery, and porcine intestinal spirochetosis, respectively. Conventional PCR is the major diagnostic tool in the detection of the three pathogens, but the sole detection of bacterial DNA might lead to misinterpretations of results with respect to their clinical relevance, especially with mixed infections. Thus, the present study targeted the detection and quantification of the three pathogens in samples from herds with a case history of diarrhoea. Herds and samples were selected by the practitioners on a voluntary basis. Results were based on 1176 individual samples from 95 herds from Southern Germany. The pathogens were detected simultaneously by multiplex real-time PCR. The overall prevalence for L. intracellularis, B. hyodysenteriae and B. pilosicoli was 12.6%, 8.4% and 3.2% in faecal samples and 48.4%, 24.2% and 31.6% in herds, respectively. Sixty one percent, 82.6%, and 73.4% of herds positive for L. intracellularis, B. hyodysenteriae, and B. pilosicoli, respectively, had mixed infections. Median log values of DNA equivalents/g of faeces for L. intracellularis, B. hyodysenteriae and B. pilosicoli were 3.3, 5.9 and 3.2, with maxima of 8.3, 8.0 and 6.3, respectively. Within herd prevalence of B. hyodysenteriae and B. pilosicoli as well as the load of B. hyodysenteriae were significantly associated with the severity of diarrhoea.  相似文献   

Feline leukemia virus and feline immunodeficiency virus article - a comment     

Ravi M  Wobeser G  Taylor S  Jackson M 《The Canadian veterinary journal. La revue veterinaire canadienne》2011,52(11):1161; author reply 1161

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Isoproturon mineralization in an agricultural soil     

Talaat El Sebaï  Marion Devers-Lamrani  Bernard Lagacherie  Nadine Rouard  Guy Soulas  Fabrice Martin-Laurent 《Biology and Fertility of Soils》2011,47(4):427-435

The impact of soil moisture content and temperature on isoproturon (3-(4-isopropylphenyl)-1,1-dimethyl-urea [IPU]) mineralization activity was assessed on an agricultural soil regularly exposed to this herbicide. Mineralization of ¹⁴C-IPU was monitored on soil microcosms incubated at different temperatures (10°C, 20°C, 28°C) and soil moisture contents (9%, 12%, 15, 18%, 21%, 24%). An increase in temperature and/or soil moisture significantly enhanced the maximum rate and percentage of IPU mineralization while it decreased the lag time before mineralization. The maximum rate and percentage of IPU mineralization respectively ranged from 0.18% day⁻¹ and 9% for the lowest temperature and soil moisture content pair (10°C–9%) to 1.51% day⁻¹ and 27.1% for the highest pair (28°C–24%). Statistics revealed a cross interaction of temperature and soil moisture content on the maximum rate of IPU mineralization. The optimum conditions for IPU mineralization, estimated from the double Gaussian model, were 25.8°C and 24% soil moisture content. The influence of fluctuations in soil moisture content on IPU-mineralization was investigated by subjecting the soil microcosms to drought stress. When IPU was added at the end of the drought stress, it had no statistical effect on IPU mineralization. However, when it was added before the drought stress, two mineralization phases were observed: (1) one corresponding to the drought stress for which mineralization was low and (2) another one observed after restoration of soil moisture content characterized by higher mineralization rate. It can be concluded that climatic fluctuations affect the activity of IPU mineralizing microbial community, and may lead to an increase in IPU persistence.  相似文献   

Phenolic composition and antioxidant properties of poplar bud (Populus nigra) extract: individual antioxidant contribution of phenolics and transcriptional effect on skin aging     

Dudonné S  Poupard P  Coutière P  Woillez M  Richard T  Mérillon JM  Vitrac X 《Journal of agricultural and food chemistry》2011,59(9):4527-4536

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