The GISVET’04 Special Edition     

Peter Durr  Wayne Martin   《Preventive veterinary medicine》2005,71(3-4):137-140

  相似文献   

Non-structural plum pox potyvirus proteins detected by immunogold labelling     

Martin  M.T.  G&#;lie  B. 《European journal of plant pathology / European Foundation for Plant Pathology》1997,103(5):427-431

Plum pox potyvirus (PPV) induces in infected Nicotiana clevelandii cells characteristic crystalline inclusions known as nuclear inclusions (NI) when located in the nucleus and as dense material (Dm) when located in the cytoplasm. Crystalline inclusions contain protease (NIa) and RNA-dependent RNA polymerase (NIb) proteins. It is now well established for all potyviruses that cylindrical inclusions contain CI helicase ATPase protein (Martin et al., 1992). The intracellular location of other non-structural PPV proteins remains unknown. Using Escherichia coli expression vectors, specific antibodies were obtained against P1, P3, 6K2 and NIb PPV proteins for which antibodies were not yet available. As expected, NIb antiserum labelled crystalline inclusions. P1, P3 and 6K2 proteins were present in both types of crystalline inclusions found in the nucleus and in the cytoplasm of PPV-infected leaves of N. clevelandii, suggesting that nuclear inclusions and dense material were composed of the same proteins. This composition is discussed.  相似文献   

Evaluating the measuring software package Metron-PX for morphometric description of equine hoof radiographs     

Jairo Vargas Rocha DVM  Christophorus J. Lischer DVM Habil  ECVS  Martin Kummer DVM  Michael Hssig DVM Habil  MPH  FVH  ECBHM  Jrg Andreas Auer DVM Prof  ACVS  ECVS 《Journal of Equine Veterinary Science》2004,24(8):347-354

  相似文献   

Immunohistochemical differentiation of reactive from malignant mesothelium as a diagnostic aid in canine pericardial disease          下载免费PDF全文

E. Milne  Y. Martinez Pereira  C. Muir  T. Scase  D. J. Shaw  G. McGregor  L. Oldroyd  E. Scurrell  M. Martin  C. Devine  H. Hodgkiss‐Geere 《The Journal of small animal practice》2018,59(5):261-271

Objectives

To develop a provisional immunohistochemistry panel for distinguishing reactive pericardium, atypical mesothelial proliferation and mesothelioma in dogs.

Materials and Methods

Archived pericardial biopsies were subject to haematoxylin and eosin staining, immunohistochemistry for cytokeratin, vimentin, insulin‐like growth factor II mRNA‐binding protein 3, glucose transporter 1 and desmin. Samples were scored for intensity and number of cells stained.

Results

Ten biopsies of reactive mesothelium, 17 of atypical mesothelial proliferation, 26 of mesothelioma and five of normal pericardium were identified on the basis of haematoxylin and eosin staining. Cytokeratin and vimentin were expressed in all biopsies, confirming mesothelial origin. Normal pericardial samples had the lowest scores for insulin‐like growth factor II mRNA‐binding protein 3, glucose transporter 1 and desmin. Mesothelioma and atypical proliferative samples were similar to each other, with higher scores for insulin‐like growth factor II mRNA‐binding protein 3 and glucose transporter 1 than the reactive samples. Desmin staining was variable. Insulin‐like growth factor II mRNA‐binding protein 3 was the best to distinguish between disease groups.

Clinical Significance

An immunohistochemistry panel of cytokeratin, vimentin, insulin‐like growth factor II mRNA‐binding protein 3 and glucose transporter 1 could provide superior information compared with haematoxylin and eosin staining alone in the diagnosis of cases of mesothelial proliferation in canine pericardium, but further validation is warranted.  相似文献   

Laryngeal mask airway and transient hypercapnic hyperpnea for video‐endoscopic assessment of unilateral laryngeal paralysis in dogs          下载免费PDF全文

Daniel M. Sakai MV  DACVAA  Manuel Martin‐Flores MV  DACVAA  Amanda K. Jones PhD  Galina M. Hayes BVSc  PhD  DACVECC  DACVS‐SA  Marina J. McConkey DVM  DACVS‐SA  Jonathan Cheetham VetMB  PhD  DACVS‐LA 《Veterinary surgery : VS》2018,47(4):543-548

  相似文献   

Knowledge,attitudes and practices towards <Emphasis Type="Italic">cystic echinococcosis</Emphasis> in livestock among selected pastoral and agro-pastoral communities in Uganda     

Leonard Omadang  Martin Chamai  Emmanuel Othieno  Andrew Okwi  Francis Olaki Inangolet  Francis Ejobi  Peter Oba  Michael Ocaido 《Tropical animal health and production》2018,50(1):11-17

A cross-sectional study was done from March 2013 to May 2014 to assess knowledge, attitudes, and practices towards cystic echinococcosis (CE) or hydatidosis among selected pastoral and agro-pastoral communities in Uganda. A structured questionnaire was administered to 381 respondents. Multivariate logistic regression analysis was done to find the relationship between knowledge about CE and factors such as age, sex, and level of education across all regions. The odds ratio and confidence interval were used to determine the difference in responses across regions. It was shown that age above 36 years was significantly (p < 0.001) associated with awareness about CE in livestock. Likewise, uneducated (p < 0.0001) and agro-pastoralists (p = 0.01) were significantly less knowledgeable than the educated and pastoralists across all regions. The overall knowledge towards CE in livestock was low 17.8% (95% CI = 14.0–21.6). Dog ownership was high and they never dewormed their freely roaming dogs. Dogs shared water with livestock. In conclusion, knowledge about CE in livestock was low across all regions. Therefore, public health education and formulation of policies towards its control by the relevant stakeholders should be done. Also, the true prevalence of CE in livestock needs to be done so that the magnitude and its public health significance are elucidated.  相似文献   

Changes in the density and growth of brown trout (Salmo trutta) after intensive removal of sympatric Arctic charr (Salvelinus alpinus) in the sub‐Arctic lake Møkkelandsvatn,Norway          下载免费PDF全文

Martin A. Svenning  Øyvind K. Hanssen  J. Malcolm Elliott 《Ecology of Freshwater Fish》2015,24(4):591-599

This study tests the basic hypothesis that the removal of charr, Salvelinus alpinus (L.), would cause an increase in both the growth and density of a sympatric trout population, Salmo trutta L. The charr population was characterised by slow‐growing individuals, with a high proportion of mature fish, that is typical for so‐called overpopulated populations. A total of 31,000 charr was removed from the lake in the period 1990–1992, and the density of younger trout (1+, 2+), but not older trout (3+, 4+), increased. The growth of older trout (3+, 4+) increased, but the evidence for similar growth increases of younger trout (1+, 2+) was limited. From 1989 to 1990, the proportion of trout increased from 30 to only 40% of the total catch, but from 1991 to 1994, it was significantly higher (60–80%) than that of charr. Total trout biomass increased to a maximum in 1992 and then decreased so that the biomass of 1994 was nearly similar to that of 1989, that is before the start of the charr removal. Back‐calculated lengths of trout from otoliths showed that 2+ and 3+ trout caught in the pelagic were growing consistently faster over previous years than those caught in the littoral, while this was not the case for the 4+ fish. Therefore, the hypothesis was partially supported; the growth rate of trout increased (age groups 1+ to 4+), while the density of juvenile trout (1+, 2+), but not the older trout (3+, 4+), increased after the removal of charr.  相似文献   

Associations of fish with various types of littoral habitats in reservoirs     

Marek Šmejkal  Marie Prchalová  Martin Čech  Mojmír Vašek  Milan Říha  Tomáš Jůza  Petr Blabolil  Jan Kubečka 《Ecology of Freshwater Fish》2014,23(3):405-413

Fish associations with different types of littoral habitats were studied in four canyon‐shaped reservoirs in the Czech Republic in years 2010 and 2011 by gillnets. Two to three habitats per reservoir–beaches (former meadows), stump fields (former forest) and rubble slopes–were defined and sampled along the longitudinal axis of reservoirs. Effects of reservoir, habitat and locality (position along longitudinal axis) on fish biomass, abundance and species structure were tested for juvenile and adult fish separately. Hierarchical analysis of variance revealed that habitats differed significantly in fish biomass and abundance. Redundancy analysis showed that analysed environmental variables had significant influence on fish community structure. Most variability in community structure was explained by reservoir and then by combination of habitat and slope steepness. Locality position had the smallest influence on community structure. For both adult and juvenile fish total abundance and biomass, the most inhabited habitat was beaches; rubble slopes were the least inhabited. Habitat associations differed among species. Among adults, bream Abramis brama, white bream Blicca bjoerkna and roach Rutilus rutilus were associated with beaches and stump fields, whereas perch Perca fluviatilis, ruffe Gymnocephalus cernuus, asp Aspius aspius and pike Esox lucius were associated with rubble slopes. Bream, white bream, bleak Alburnus alburnus, roach, ruffe and pikeperch Sander lucioperca were associated with beaches among juveniles, whereas the only juvenile associated with rubble slopes was perch. We showed that most common species are associated with distinct habitats and also that utilisation of various littoral habitats differs in general.  相似文献   

Dauerdüngungsversuche als grundlage für nachhaltige landnutzung und quantifizierung von Stoffkreisläufen: Vorwort     

Wolfgang Merbach  Martin Körschens 《Archives of Agronomy and Soil Science》2013,59(3-4):175-176

  相似文献   

Comparison of bupivacaine and dexmedetomidine femoral and sciatic nerve blocks with bupivacaine and buprenorphine epidural injection for stifle arthroplasty in dogs          下载免费PDF全文

Annatasha KG Bartel  Luis Campoy  Manuel Martin‐Flores  Robin D Gleed  Kyle J Walker  Courtney E Scanapico  Alison B Reichard 《Veterinary anaesthesia and analgesia》2016,43(4):435-443

  相似文献