西昆仑山2个海拔位点可培养细菌多样性分析     

玛丽帕·吐达洪  阿不都外力·阿布都热依木  孜来古丽·米吉提  卡依尔·玉素甫  马丽艳木·阿木东  艾尼江·尔斯曼  祖母拉提·阿布都热依木  艾尔肯·热合曼 《干旱区研究》2011,28(6):1071-1080

以西昆仑山2个海拔样点的土样为对象,利用（LB和TSA）2种培养基,基于16S rRNA基因序列的系统发育,采用纯培养法对其可培养细菌多样性进行研究。2样点中共分离到91株细菌,分为25个属,52个种。厚壁菌门（Firmicutes）占总菌数的48.75%,放线菌门（Actinobacteria）占36.25%,γ-变...  相似文献   

Isolation and identification of bubaline herpesvirus 1 (BuHV-1) from latently infected water buffalo (Bubalus bubalis) from Iran     

Hedayat  Negar  Haji Hajikolaei  Mohammad Rahim  Seyfi Abad Shapouri  Masoud Reza  Ghadrdan Mashhadi  Ali-Reza  Izadnia  Hamidreza  Daghari  Maryam 《Tropical animal health and production》2020,52(1):217-226

In order to isolate buffaloes herpesvirus 1 (BuHV-1) from latently infected water buffalo (Bubalus bubalis), 16 buffalo heifers were selected from a herd. At first, animals were bled and their sera were tested by virus neutralization (VN) test, using bovine herpesvirus 1 (BoHV-1). According to the results of VN test and dexamethasone injection (0.1 mg/kg BW) for 5 consecutive days, the examined buffaloes were divided into 4 groups. Vaginal and nasal swabs were daily collected from all buffaloes from day 0 to 10 days later. Based on the cytopathic effects in cell culture, a herpesvirus was isolated only from nasal swabs of three seropositive buffaloes which they had received dexamethasone. The nasal swabs of these three buffaloes were also positive in PCR, using primers specific for ruminant herpesviruses gD gene. The identity of the isolated viruses was determined according to partial amino acid sequences of gD, deduced from the nucleotide sequences of the PCR products. On the basis of sequence alignment, phylogenetic analysis, and genetic distances, the three buffalo virus isolates were more closely related to BuHV-1 and BoHV-5 than to BoHV-1.

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Phylogenetic relationships of Fusarium oxysporum f. sp. melonis in Iran     

Maryam Mirtalebi  Zia Banihashemi  Celeste C. Linde 《European journal of plant pathology / European Foundation for Plant Pathology》2013,136(4):749-762

Fusarium wilt of melon caused by Fusarium oxysporum f. sp. melonis is a destructive fungal disease in melon growing regions. Isolates of F. oxysporum obtained from six major melon producing provinces in Iran, from melons and other hosts, were characterized based on pathogenicity to melon, vegetative compatibility groups (VCGs) and nuclear ribosomal DNA intergenic spacer (IGS) sequencing. Thirty-four of 41 isolates from Iran in this study were identified as race 1,2 which belonged to either VCG 0134 or an unassigned VCG, which based on IGS sequencing grouped with the VCG 0135 tester isolate. The seven remaining isolates were identified as nonpathogenic to melon belonging to two undescribed VCGs. Based on sequence analyses of the IGS region of Iranian and foreign isolates, nine lineages were identified, each including one VCG. The separation of VCGs into distinct lineages based on IGS sequences is mostly consistent with Repetitive extragenic palindromic PCR (Rep-PCR) results. Exceptions are VCGs 0130 and 0131, which could be differentiated with IGS sequences, but not with Rep-PCR. Different races from the USA, France and Iran associated with VCG 0134 grouped into one IGS lineage but could be differentiated with Rep-PCR, suggesting that this VCG is more diverse than previously thought. Given the long history of melon cultivation in Iran and the Rep-PCR diversity of isolates belonging to this VCG, it could be speculated that VCG 0134 perhaps evolved in Iran.  相似文献   

Genetic and genomic dissection of Prolactin revealed potential association with milk production traits in riverine buffalo     

A. Nadeem  J. Maryam 《Tropical animal health and production》2016,48(6):1261-1268

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Effects of drought stress and rewatering on antioxidant systems and relative water content in different growth stages of maize (Zea mays L.) hybrids     

Maryam Goodarzian Ghahfarokhi  Syrus Mansurifar  Ruhollah Taghizadeh-Mehrjardi  Mohsen Saeidi  Amir Mohammad Jamshidi  Elahe Ghasemi 《Archives of Agronomy and Soil Science》2013,59(4):493-506

Drought is a problem of the expanding universe which seriously influences crop production and quality. Approximately one-third of the cultivated area of the world suffers from constantly inadequate supplies of water. The present study aimed to determine the effects of drought and rewatering on activities of antioxidant enzymes, chlorophyll, proline, and relative water contents (RWC). In this experiment, six maize hybrids (Sc260, Sc370, Sc500, Sc647, Sc700, and Sc704) were examined in a pot study during the maize-growing season of 2011. Results indicated that the growth of hybrids was retarded under drought stress conditions and regained growth speed during rewatering. RWC, chlorophyll, and carotenoid contents were markedly decreased by the water deficit and reached normal values during rewatering in Sc647, Sc704, and Sc700. Our findings also indicated significantly higher activity levels of peroxidase and catalase and proline content in water-stressed plants than in well-watered plants, which decreased when the plants were rewatered, showing an inverse relationship to gluthatione reductase activity. According to the results, the better upregulation of the protective mechanism in Sc704 and Sc647 probably induced higher drought resistance. We concluded that antioxidant enzyme activity could provide a useful tool for depicting drought tolerance in maize hybrids in arid and semiarid regions.  相似文献   

Heavy metal uptake and translocation by Justicia gendarussa Burm F. from textile sludge contaminated soil     

Nik Muhamad Majid  M. M. Islam  Melina E. Nap  Maryam Ghafoori  Arifin Abdu 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2013,63(2):101-108

Abstract

Heavy metals are dangerous environmental pollutants that can be transferred and accumulated in human and animal bodies causing deoxyribonucleic acid (DNA) damage and carcinogenic effects. A glass house experiment was conducted to evaluate the potential of Justicia gendarussa Burm F. to absorb heavy metals from textile industry sludge. Justicia gendarussa seedlings were planted on six different growth media (soil+sludge) comprising: 100% soil, 100% sludge, 80% sludge+20% soil, 60% sludge+40% soil, 40% sludge+60% soil and 20% sludge+80% soil. The maximum height increment and number of leaves were found in 20% sludge+80% soil while the highest basal diameter increment was recorded in the 100% sludge. Copper and iron were highly concentrated in the roots, zinc in the leaves, while aluminium was concentrated in both leaves and stems. Justicia gendarussa seems to have a high potential to absorb high amounts of Al and Fe in the leaves and roots. This species showed high translocation (TF) and low bioconcentration factor (BCF) in the contaminated soil. Justicia gendarussa was able to tolerate and accumulate a high concentration of heavy metals. Therefore, this species can be considered as a potential phytoremediator.  相似文献   

Amendment of hydroponic nutrient solution with humic acid and glutamic acid in tomato (Lycopersicon esculentum Mill.) culture     

Maryam Haghighi  Jaime A. Teixeira Da Silva 《Soil Science and Plant Nutrition》2013,59(4):642-648

Abstract

Can humic acid (HA) and glutamic acid (GA), when added to tomato (Lycopersicon esculentum Mill. cv. ‘Hongyangli’) nutrient solution in a hydroponic system, improve growth? Tomato seedlings were grown in six nutrient solutions: (1) control (C), (2) C + 25 mg L^?1 HA (HA1); (3) C + 50 mg L^?1 HA (HA2); (4) C + 100 mg L^?1 GA; (5) HA1 + GA; (6) HA2 + GA. Various biochemical and physiological parameters were measured. HA increased photosynthesis rate and mesophyll conductance. HA did not significantly affect transpiration, stomatal conductance, titratable acidity, or antioxidant activity. In addition, GA improved protein and sugar content, mesophyll conductance and yield. The combination of HA and GA was more effective, especially with 50 mg L^?1 HA. The activity of superoxide dismutase (SOD) and peroxidases (POD) did not change in the presence of HA or GA. Malondialdehyde (MDA) content increased by 30% in HA2 together with GA. HA has a positive effect on tomato hydroponic growth when applied with GA. This expands the use of HA and GA for horticultural commodities in hydroponic systems.  相似文献   

Role of Penconazole in salt stress amelioration in Sesamum indicum L.     

Hamideh Heydari  Maryam Rezayian  Hassan Ebrahimzadeh 《Soil Science and Plant Nutrition》2013,59(3):243-250

ABSTRACT

Sesame (Sesamum indicum L.) is one of the world’s important oil crops. The aim of the present study was to investigate the effects of salt stress on physiological and biochemical parameters of sesame and assess the improvement of salt tolerance by Penconazole (PEN). Plants were treated with various NaCl concentrations (0, 50, 100, and 200 mM) without or with PEN (15 mg L^?1). Salt stress inhibited the growth in sesame. Our results showed sesame plant under salinity used of the enzymatic and non-enzymatic system and compatible osmolytes to overcome on oxidative and osmotic stress, respectively. PEN improved the growth and reduced the negative effects of salinity in sesame. In fact, PEN increased the resistance of this plant under salt stress by improving the antioxidant system and osmotic regulation. Thus, this compound can be used as stress-ameliorating agents in this crop plant.  相似文献   

Phenotypic diversity and relationships of fruit quality traits in inter-specific almond × peach backcrosses breeding progenies     

Hamid Yaghini  Maryam Shirani  Azin Archangi  Karim Sorkheh  Sajad Badfar Chaleshtori  Seyed Ehsan Sangi  Mahmood Khodambashi  Farahnaz Tavakoli 《Euphytica》2013,194(3):305-324

Nineteen agrochemical traits of 20 almond inter-specific backcrosses progenies were evaluated and compared for three consequence years to find out their phenotypic diversity and determine the relationships of fruit quality traits in almond × peach backcrosses breeding progenies. The variation was observed for traits of phenology parameters (blooming time, ripening time), Physical parameters (fruit weight, width, height, shape, thickness, skin pubescences, colour and flower type), chemical parameters (total sugar content, soluble solids content and acidity) and sensory parameters (attractiveness, taste, and flavor) and yield. Many fruit characteristics that are important to breeders are present in this collection. A high variability was found in the evaluated almond progenies and significant differences were found among them in all studied quality attributes. Year-by-year variations were observed for majority of traits. A significant correlation was found among the fruit height, fruit width, skin pubescences and yield. Fruit height showed a significant positive correlation with fruit weight and fruit thickness and some other traits and a negative correlation with the titratable acidity, skin pubescences and fruit flavour. A high negative correlation was found between the fruit weight and titratable acidity (?0.8). Low coefficients were got between the flower colour and skin pubescences. In addition, principal component analysis it possible to established similar groups of genotypes depending on their quality characteristics and to study relationships among pomological traits in almond progenies evaluated.  相似文献   

Expression analysis of the genes involved in osmotic adjustment in bread wheat (Triticum aestivum L.) cultivars under terminal drought stress conditions     

Hamid Hoseinian Khoshro  Alireza Taleei  Moahmmad Reza Bihamta  Maryam Shahbazi  Alireza Abbasi 《Journal of Crop Science and Biotechnology》2013,16(3):173-181

Osmotic adjustment is the main component for physiological machinery of wheat drought tolerance. Some of parameters implicated in osmotic adjustment in 15 bread wheat cultivars were evaluated at soil water deficits (50% FC) and FC as the control in the greenhouse. For the physiological traits, analysis of variance showed that there are highly significant differences between treatments, i.e. water stress levels and wheat cultivars. Shahpasand and Marvdasht as sensitive wheat cultivars had significantly lower osmotic adjustment, relative water content, K+ content, soluble sugar, proline, and glycine betaine levels than the rest of the cultivars. The results of cluster analysis revealed that all cultivars were grouped into three distinct clusters. Dez, Kavir, Pishtaz, and Maron cultivars which have the highest osmoregulation activity were in cluster I, whereas clusters II (Pishgam, Aflak, Hirmand, Zagros, and Vee/Nac) and III (Ws-89-2, Sardari, Azar2, Shapasand, and Marvdasht) had intermediate activity and the lowest capacities for osmoregulation, respectively. In addition, in wheat flag leaf during the reproductive stage under drought conditions, the changes in gene expression of two key genes namely P5CS (D-Pyrroline-5-carboxylate synthetase) and BADH (Betaine Aldehyde Dehydrogenase) in two selected cultivars including Dez and Marvdasht revealed that water stress can increase the expression level of the genes P5CS and BADH in the resistant cultivar, Dez, compared with Marvdasht, the sensitive one. In general, it seemed that application of the all cultivars in cluster I would enable breeders to acquire more reliable achievements under drought conditions.  相似文献