Phylogeography of the Japanese eight-barbel loach <Emphasis Type="Italic">Lefua echigonia</Emphasis> from the Yamagata area of the Tohoku district,Japan     

Hiroshi Aiki  Katsuhiko Takayama  Takashi Tamaru  Nobuhiro Mano  Masafumi Shimada  Hiroshi Komaki  Hitomi Hirose 《Fisheries Science》2009,75(4):903-908

The Japanese eight-barbel loach Lefua echigonia, which is a freshwater fish native to Japan, is distributed from the Tohoku to Kinki districts and is divided into six regional populations according to mtDNA analysis. In this study, we investigated L. echigonia collected from several locations in Yamagata Prefecture and neighboring prefectures using mtDNA control region sequences and confirmed the spatial distribution pattern among the new regional population (Yamagata population). The new population was limited to the Mogami river system in the inland area of Yamagata Prefecture and is distinguished from other regional populations by high sequence divergences.  相似文献   

Studies on Endophytic Actinomycetes ( I ) <Emphasis Type="Italic">Streptomyces </Emphasis>sp. Isolated from Rhododendron and Its Antifungal Activity     

Masafumi SHIMIZU  Yoshiko NAKAGAWA  Yukio SATO  Tamotsu FURUMAI  Yasuhiro IGARASHI  Hiroyasu ONAKA  Ryuji YOSHIDA  Hitoshi KUNOH 《Journal of General Plant Pathology》2000,66(4):360-366

To survey endophytic actinomycetes as potential biocontrol agents against fungal diseases of rhododendron, young plants of rhododendron were surface-sterilized for use as an isolation source. Nine, six and two isolates, with distinguishing characteristics based on the macroscopic appearance of colonies, were obtained from roots, stems and leaves, respectively, suggesting that various species of actinomycetes grow in the respective organs of this plant as symbionts or parasites. On an agar medium, only isolate R-5 commonly formed a clear growth-inhibition zone against two major fungal pathogens of rhododendron, Phytophthora cinnamomi and Pestalotiopsis sydowiana, indicating that this isolate can produce antifungal material(s). Acetone extracts of a liquid culture of R-5 had a broad antimicrobial spectrum against Gram-positive bacteria, yeast and filamentous fungi. Isolate R-5 was identified as a Streptomyces sp. based on morphological, physiological and chemotaxonomical characteristics. The present results indicate that isolate R-5 is a suitable candidate for the biocontrol of diseases of rhododendron. Received 25 March 2000/ Accepted in revised form 18 May 2000  相似文献   

Interaction of orexin/hypocretin-like immunoreactive neurons with melanin-concentrating hormone and α-melanocyte-stimulating hormone neurons in brain of a pleuronectiform fish, barfin flounder   总被引：1，自引：0，他引：1  

Noriko Amiya  Masafumi Amano  Masayuki Iigo  Takeshi Yamanome  Akiyoshi Takahashi  Kunio Yamamori 《Fisheries Science》2008,74(5):1040-1046

Immunohistochemical localization of orexin/hypocretin in the brain of a pleuronectiform fish, the barfin flounder Verasper moseri was examined as the first step in unraveling the possible function of the hormone in the brain. Orexin-A-like immunoreactive (ir) cell bodies were found to be located in the nucleus posterioris periventricularis (NPPv) of the hypothalamus, and orexin-A-like-ir fibers were detected not only in the hypothalamus but also extensively throughout the brain. The orexin-A-like-ir cell bodies did not project their fibers to the pituitary gland. Since melaninconcentrating hormone (MCH) and α-melanocyte-stimulating hormone (α-MSH) are suggested to regulate food intake in addition to orexin/hypocretin in the teleost fish, it was examined whether neural connections exist between orexin neurons and the MCH and α-MSH neurons in the barfin flounder brain by using double-staining immunohistochemistry. Some orexin-A-like-ir fibers were in close contact with the MCH-ir and α-MSH-ir cell bodies in the hypothalamus. Moreover, a few MCH-ir and α-MSH-ir fibers were in close contact with the orexin-A-like-ir cell bodies in the hypothalamus. These results suggest that reciprocal connections exist between the orexin and MCH neurons and between the orexin and α-MSH neurons in the brain of the barfin flounder.  相似文献   

A single gene transfer of gibberellin biosynthesis gene cluster increases gibberellin production in a Fusarium fujikuroi strain with gibberellin low producibility     

WanXue Bao  Takuya Nagasaka  Shin Inagaki  Sho Tatebayashi  Iori Imazaki  Shin-ichi Fuji  Takashi Tsuge  Masafumi Shimizu  Koji Kageyama  Haruhisa Suga 《Plant pathology》2020,69(5):901-910

Fusarium fujikuroi, the causative agent of bakanae disease in rice, produces many kinds of secondary metabolites. Recently, two phylogenetic subgroups (F and G groups) of Japanese F. fujikuroi have been identified and found to have differences in their gibberellin (GA) and fumonisin production. G-group F. fujikuroi produces large amounts of GA, but is a fumonisin nonproducer. F-group produces large amounts of fumonisin, but is a GA low or nonproducer. We investigated the cause of low GA production in the F-group. Genetic mapping suggests that low GA production in the F-group strain Gfc0825009 is due to a GA gene cluster for GA biosynthesis. Analysis of the nucleotide and amino acid sequences of the genes in the GA gene cluster showed >98.4% homology between the F-group strain Gfc0825009 and the G-group strain Gfc0801001. Following a 7-day culture under low nitrogen conditions, we found that expression of P450-1, P450-4, and P450-2 in the cluster increased in the G-group strain and not in the F-group strain. We hypothesized that complementation by GA genes in the G-group strain would be required to increase GA production in the F-group strain. However, we found that this occurred with a single gene complementation of DES, P450-1, P450-4, or P450-2. Simultaneous increase in the expression of P450-1, P450-4, and P450-2 were detected in the complementary transformants. Moreover, the same phenomenon was observed by reintegration of its own P450-1. Our results suggest the presence of unknown regulatory mechanisms of the GA gene cluster in F. fujikuroi.  相似文献   

Is yield enhancement by CO₂ enrichment greater in genotypes with a higher capacity for nitrogen fixation?     

Masafumi OteraMakie Kokubun  Hiroko TabeiToshinori Matsunami  Tomiya MaekawaMasumi Okada 《Agricultural and Forest Meteorology》2011,151(10):1385-1393

Yield responsiveness to elevated CO₂ concentration [CO₂] was previously found to be greater when nitrogen (N) was supplied in adequate amounts; however, it remains unclear whether genotypic differences in N₂-fixing capacity affect yield responsiveness in soybean. We tested the hypothesis that yield responsiveness to elevated [CO₂] in soybean is greater in a genotype with a higher capacity for N₂ fixation. We used three near-isogenic genotypes with contrasting nodulation capacities: super-nodulating, normally nodulating and non-nodulating genotypes. Plants were subjected to two levels of [CO₂] (ambient or elevated: ambient + 200 μmol mol⁻¹) and two temperature regimes (low or high: low + ca. 4-5 °C) using temperature gradient chambers. The super-nodulating genotype exhibited a higher N content in leaves, regardless of [CO₂] and temperature. Photosynthetic rates were enhanced by CO₂ enrichment at earlier growth stages, but not at later growth stages, regardless of genotype. This photosynthetic acclimation was reflected in biomass production in all the genotypes examined. Yield responsiveness to elevated [CO₂] was greater in the nodulating genotypes than in the non-nodulating genotype, but the genotypic differences were obscured between the normally nodulating and super-nodulating genotype, thus our hypothesis was not fully verified.  相似文献   

Development of new self-feeding system for mass rearing of ayu <Emphasis Type="Italic">Plecoglossus altivelis altivelis</Emphasis> under artificial and natural light-dark cycles     

Masafumi Amano  Masayuki IIgo  Toshikazu Sunuma  Mitsushi Yamashita  Kiyoshi Furukawa  Mitsuo Tabata  Kunio Yamamori 《Fisheries Science》2007,73(4):800-807

Self-feeding of grouped ayu Plecoglossus altivelis altivelis was examined under an artificial light-dark (LD) cycle and natural day length using a trigger with a photosensitive sensor. In Experiment 1, approximately 15 juvenile fish (mean body weight, 0.6 g) were kept in 60-L glass tanks (four tanks) under LD 12∶12 (lighting period 06∶00–18∶00 hours) and self-feeding and locomotor activities were simultaneously recorded. Self-feeding was detected within one day and feeding activities were strongly synchronized to the LD cycle, with almost strictly diurnal feeding (98.2%). Although locomotor activities in the upper part of the tank were also greatest during the light phase (76.8%), the activity profiles were different from those of self-feeding, suggesting the validity of the photosensitive sensor. In Experiment 2,25 fish (mean body weight, 15 g) were kept in outdoor 1000-L tanks (four tanks) under natural day length and water temperatures in May, and self-feeding was recorded from mid-August. Stable self-feeding was observed from early September in all the tanks. Nearly all food demands were diurnal, and usually crepuscular. Somatic growth was seen in all tanks. These results demonstrate that the self-feeding system using a photosensitive sensor is applicable for grouped juvenile ayu as small as 0.6 g and adult fish reared under natural conditions.  相似文献   

Effects of feeding time on approaching behavior to food odor in goldfish     

Masafumi Amano  Masayuki IiGo  Kunio Yamamori 《Fisheries Science》2005,71(1):183-186

ABSTRACT:   Effects of feeding time on approaching behavior to food odor in goldfish Carassius auratus were examined using the Y-maze flow-through system to test whether the behavior differed according to feeding time and experimental time. Fish were fed at 08.00, 12.00 or 16.00 hours once a day or three times (08.00, 12.00 and 16.00 hours) a day for at least 3 weeks under a natural photoperiod, and at 22 ± 2°C, preference reactions to food odor were tested at 08.00, 10.00, 12.00, 14.00 and 16.00 hours. The fish fed once a day showed significant positive approaching behavior only at the time when they were normally fed. The fish fed three times a day showed no significant positive approaching behavior. These results suggest that goldfish can remember when food is available and/or that there exists a mechanism to inhibit approaching behavior when food is not available.  相似文献   

Effects of hypoxia on principal prey and growth of flathead flounder Hippoglossoides dubius in Funka Bay, Japan     

Masafumi  KIMURA  Toyomi  TAKAHASHI  Tetsuya  TAKATSU  Toshikuni  NAKATANI  Tatsuaki  MAEDA 《Fisheries Science》2004,70(4):537-545

ABSTRACT:   Effects of hypoxia on the principal prey and growth of flathead flounder Hippoglossoides dubius were studied in Funka Bay. Of the three dominant year-classes that occurred in recent years, the 1995 year-class was small in total length at age ≥ 3 and low in condition factor at age ≥ 2. Ophiuroids (almost Ophiura sarsi ), which were the dominant prey in the 1980s, were of little importance and instead, crustaceans such as mysids, natant decapods and pelagic amphipods, bivalves and fish were important prey items for H. dubius in 2000–2001. In addition, the feeding intensity of H. dubius in 2000–2001 was lower than that in the 1980s. These facts are closely related to a reduction of prey abundance, particularly ophiuroids. It seems that the hypoxia that occurred in the central part of the bay during the summer and autumn of 1995–1997 caused the poor food supply and low growth rate at ages 2–4 of the 1995 year-class.  相似文献   

Measurement of diurnal changes in stem and branch diameters using strain gauges     

Masafumi Ueda  Ken Yoshikawa  Jiro Okitu 《Journal of Forest Research》1996,1(3):139-142

The applicability of a strain gauge for monitoring the diurnal changes of tree stem diameters and the differences in diurnal shrinkages between a stem and branches were investigated. Estimates of stem diameter changes obtained by a strain gauge were compared with changes obtained by a differential transformer. Patterns of strain changes obtained by a strain gauge were remarkably similar to the changes in the tree trunk diameter. This results demonstrated that a strain gauge is an effective technique for examining the behavior of changes in tree trunk diameter. Diurnal changes of diameter at various parts of the a tree were also measured with the help of several strain gauges. Diurnal strain changes of a stem and branches showed similar patterns of shrinkage during the day and swelling at night. However, the precise times at which the branches had their maximum and minimum strains differed from those observed in the stem, and the maximum rates of the strain changes lagged behind the stems in a day. These results lead us to believe that there is a time lag in the supply of water, which is required for compensating the water deficit between the stem and its branches. A part of this paper was presented at the 105th Annual Meeting of Japanese Forestry Society (1994).  相似文献   

Water removal of wet veneer by roller pressing   总被引：2，自引：0，他引：2  

Koji?AdachiEmail author  Masafumi?Inoue  Kozo?Kanayama  Roger M.?Rowell  Shuichi?Kawai 《Journal of Wood Science》2004,50(6):479-483

High moisture content, flat sawn Japanese cedar (Cryptomeria japonica D. Don) veneer was compressed using a roller press to mechanically remove water. The amount of water removed depended on the amount of compression applied. At 60% compression, 400kg/m³ of water was removed. The process was not dependent on the size of the wood, the degree of compression, or the feed speed of the specimen. After compression, the remaining water contents were evenly distributed throughout the veneer regardless of the length of the specimen. The specimens did not completely recover to original thickness. High compression ratio and low temperature intensified the reduction of thickness. The bending strength after compression decreased in an inversely proportional manner with the thickness of the specimen and the compression degree.Part of this study was presented at the 50th Annual Meeting of the Japan Wood Research Society, Kyoto, April 2000  相似文献