Relationship between Oxygen Consumption and Sex of Bovine In Vitro Fertilized Embryos   总被引：1，自引：0，他引：1  

B Agung  T Otoi  H Abe  H Hoshi  M Murakami  NWK Karja  MK Murakami  P Wongsrikeao  H Watari  T Suzuki 《Reproduction in domestic animals》2005,40(1):51-56

The present study was conducted to examine the relationship between the oxygen consumption rate and sex ratio of bovine in vitro fertilized embryos on each day of blastocyst formation. The quality of blastocysts collected on day 7, 8, and 9 after in vitro fertilization (IVF) were categorized as ranks A and B (excellent and good, respectively) based on microscopic observation of the morphology. The oxygen consumption rate and sex of individual blastocysts were evaluated using two novel techniques: scanning electrochemical microscopy (SECM) and loop-mediated isothermal amplification (LAMP), respectively. The oxygen consumption rates of embryos of rank A were significantly higher (p < 0.05) than those of rank B, irrespective of the day of blastocyst appearance after IVF. Neither did the proportion of male embryos of ranks A and B differ significantly from each other at any of the days examined, nor from the average proportion (53%). The oxygen consumption rate of embryos of rank B collected on day 8 was significantly higher (p < 0.05) in female embryos than in male embryos collected on the same day. However, there were no apparent differences of oxygen consumption rates at each day of blastocyst appearance between male and female embryos of rank A. These results indicate that the oxygen consumption rate of individual embryos reflects their quality but does not correlate with the sex ratio of embryos of excellent quality.  相似文献   

Effects of size and storage temperature on meiotic competence of domestic cat oocytes     

Otoi T  Murakami M  Ooka A  Karja NW  Suzuki T 《The Veterinary record》2001,148(4):116-118

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Comparison of various reproductive status in Sika deer (Cervus nippon) using fecal steroid analysis     

Hamasaki S  Yamauchi K  Ohki T  Murakami M  Takahara Y  Takeuchi Y  Mori Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(2):195-198

The feasibility of fecal steroid analysis for pregnancy diagnosis and sex determination were tested in sika deer (Cervus nippon). Feces were collected from captive sika deer in June (non-breeding season and late-pregnancy period) and October (breeding season), and also from the rectum of 24 female sika deer (19 pregnant and 5 non-pregnant females) shot as part of programs for population control in February and March (mid-pregnancy period). In mid- and late-pregnancy periods, fecal progesterone concentrations were significantly higher in pregnant female than in male and non-pregnant female deer. In October, fecal testosterone concentrations were higher in adult male deer, and no difference was found between young males and females. These results suggest that fecal steroid analysis would be a useful means for estimating pregnancy status and for detecting adult male among wild deer.  相似文献   

Immunization against intestinal bacterial endotoxin prevents alcoholic fatty liver in rats   总被引：4，自引：0，他引：4  

Nishimura G  Nakahara K  Misawa N  Muranaka M  Uchida K  Kuroda H  Murakami N 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(3):275-280

Accumulating evidences indicate that an endotoxin originating from intestinal gram-negative bacteria may be involved in alcohol-induced liver injury including fatty liver. Therefore, whether immunization against intestinal bacterial endotoxin blocked fatty liver induced by chronic alcohol and diet including much-unsaturated fatty acid was investigated in rats. The titer of antibody against the endotoxin increased significantly after 13 weeks of continuous immunization. Daily alcohol treatment was initiated at 12 weeks and continued for 4 weeks. Plasma glutamic pyruvic transaminase (GPT), glutamic oxaloacetic transaminase (GOT) and triglyceride (TG) levels increased significantly in non-immunized rats receiving alcohol, but not in immunized rats. Continuous alcohol treatment gradually decreased the survival rate to 60% from 13 days after beginning administration in non-immunized, but not immunized, rats. A histochemical study revealed that continuous treatment with alcohol and unsaturated fatty acids caused fatty liver in non-immunized, but not immunized, rats. This study strongly supports the hypothesis that alcohol-induced fatty liver is due to a circulating endotoxin, and suggests that immunization for endotoxin prevent the alcoholic fatty liver.  相似文献   

Immunohistochemical analysis of cyclins in canine normal testes and testicular tumors     

Murakami Y  Tateyama S  Uchida K  Yamaguchi R 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(8):909-912

The expression of cyclins A, D1, D2 and E were examined immunohistochemically in 5 canine normal testes and 31 testicular tumors, including 14 seminomas, 11 Sertoli cell tumors and 6 Leydig cell tumors. In canine normal testes, cyclin A expression was detected in spermatogonia and primary spermatocytes. This suggests that A-type cyclins may play some role in canine spermatogenesis. Cyclin A expression was also observed in 13/14 (92.9%) seminomas and 2/11 (18.2%) Sertoli cell tumors, but no positive reaction was observed in Leydig cell tumors. Parallel examinations for cyclins D1, D2 and E gave negative results in canine normal testes and testicular tumors. High levels of cyclin A expression in canine seminomas indicate that the neoplastic germ cells may be arrested at the spermatogonia and primary spermatocyte stages of differentiation.  相似文献   

Retracted: Isolation,characterization, and effect of administration in vivo,a novel probiotic strain from pig feces     

Yasuhiro Ihara  Hideki Hyodo  Shin Sukegawa  Hiroshi Murakami  Fumiki Morimatsu 《Animal Science Journal》2013,84(5):434-441

From porcine rectal swabs or feces, we isolated lactic acid bacteria and used porcine Peyer's patch cells to select them for inducibility of IgA production as an indicator of probiotic effects. The strain selected as a new probiotic was named ‘Enterococcus faecium NHRD IHARA‘. To verify the probiotic effects of this strain in vivo, 536 piglets at age 25 days were assigned to either the trial group, which administrated the strain, or the control group. An increase in IgA in the feces was observed at age 45 days (P < 0.05 compared with the control group); a significant increase in serum IgA was also observed at the end of the study (P < 0.01) in the trial group. In addition, significant differences between the groups in terms of body weight (P < 0.05) and average daily gain (P < 0.01) were observed. The rate of detection of swine‐pathogenic Escherichia coli gene in the feces tended to be lower in the trial group than in the controls. The novel probiotic strain; E. faecium NHRD IHARA may have beneficial effects on swine growth by inducing IgA production and reducing rates of colonization by pathogens in the body.  相似文献   

Structural basis of transcription initiation: an RNA polymerase holoenzyme-DNA complex     

Murakami KS  Masuda S  Campbell EA  Muzzin O  Darst SA 《Science (New York, N.Y.)》2002,296(5571):1285-1290

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Ultrasonographic features and prevalence of presumed gastric wall edema in dogs with hypoalbuminemia     

Masahiro Murakami  Hock Gan Heng  Chee Kin Lim  Nolie K. Parnell  Mario Sola 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2020,34(5):1867-1871

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Control of homeostasis of CD8+ memory T cells by opposing cytokines     

Ku CC  Murakami M  Sakamoto A  Kappler J  Marrack P 《Science (New York, N.Y.)》2000,288(5466):675-678

Memory T cells maintain their numbers for long periods after antigen exposure. Here we show that CD8+ T cells of memory phenotype divide slowly in animals. This division requires interleukin-15 and is markedly increased by inhibition of interleukin-2 (IL-2). Therefore, the numbers of CD8+ memory T cells in animals are controlled by a balance between IL-15 and IL-2.  相似文献   

Changes in mRNA of immune factors expressed by milk somatic cells of Holstein cows with hypocalcemia after calving     

Hiromichi Ohtsuka  Misa Ohsawa  Kenji Murakami  Ryo Murata  Toshihide Kato  Motoshi Tajima 《Canadian journal of veterinary research》2021,85(1):72

Changes in immune factors expressed by milk somatic cells from Holstein cows with hypocalcemia after calving were investigated in this study. Fourteen multiparous Holstein cows after their 3rd or 4th calving in one farm were used. The cows were divided into 2 groups: 7 cows needing treatment due to onset of hypocalcemia (hypocalcemia group; age = 5.53 ± 0.27 years, parity = 3.14 ± 0.14) and 7 cows without health problems (control group; age = 5.88 ± 0.31 years, parity = 3.57 ± 0.26). Milk samples were collected aseptically using a cannula and mRNA of immune factors expressed by milk somatic cells were analyzed. Milk samples (50 mL) were collected from the right rear mammary gland of cows before milking at day 1 and weeks 1, 2, 4, and 8 after calving. All milk samples showed a negative reaction to the California Mastitis Test. Levels of relative interleukin (IL)-6 and cathelicidin in the hypocalcemia group were lower than those in the control group in weeks 1 to 8. A significant difference in relative IL-6 levels was found in week 4 (P < 0.05). These results suggest that levels of IL-6 expressed by milk somatic cells may be affected by hypocalcemia in dairy cows.  相似文献