Biosystematics relationships among Marrubium L. (Lamiaceae) species in Iran     

Talebi  Seyed Mehdi  Sheidai  Masoud  Arianejad  Fatemeh  Mahdieh  Majid 《Genetic Resources and Crop Evolution》2022,69(8):2833-2847

Genetic Resources and Crop Evolution - Marrubium L., is a problematic genus of Lamiaceae family with approximately 40 taxa that some of its species grow in Iran. In the current study, we studied...  相似文献   

A Single Point Mutation within the Coding Sequence of Cholera Toxin B Subunit Will Increase Its Expression Yield     

Bita Bakhshi  Mina Boustanshenas  Masoud Ghorbani 《Iranian Biomedical Journal》2014,18(3):130-135

Background: Cholera toxin B subunit (CTB) has been extensively considered as an immunogenic and adjuvant protein, but its yield of expression is not satisfactory in many studies. The aim of this study was to compare the expression of native and mutant recombinant CTB (rCTB) in pQE vector. Methods: ctxB fragment from Vibrio cholerae O₁ ATCC14035 containing the substitution of mutant ctxB for amino acid S128T was amplified by PCR and cloned in pGETM-T easy vector. It was then transformed to E. coli Top 10F'' and cultured on LB agar plate containing ampicillin. Sequence analysis confirmed the mature ctxB gene sequence and the mutant one in both constructs which were further subcloned to pQE-30 vector. Both constructs were subsequently transformed to E. coli M15 (pREP4) for expression of mature and mutant rCTB. Results: SDS-PAGE analysis showed the maximum expression of rCTB in both systems at 5 hours after induction and Western-blot analysis confirmed the presence of rCTB in blotting membranes. The expression of mutant rCTB was much higher than mature rCTB, which may be the result of serine-to-threonine substitution at position 128 of mature rCTB amino acid sequence created by PCR mutagenesis. The mutant rCTB retained pentameric stability and its ability to bind to anti- cholera toxin IgG antibodies. Conclusion: Point mutation in ctxB sequence resulted in over-expression of rCTB, probably due to the increase of solubility of produced rCTB. Consequently, this expression system can be used to produce rCTB in high yield. Key Words: Escherichia coli, Point mutation, Cholera toxin B subunit (CTB), Protein expression  相似文献   

Growth and nutrient and nitrate accumulation of lettuce under different regimes of nitrogen fertilization     

Omid R. Zandvakili  Masoud Hashemi  Fatemeh Etemadi  Wesley R. Autio  Sarah Weis 《Journal of plant nutrition》2013,36(14):1575-1593

Abstract

For plant growth and composition, the effects of fertilizers including blood meal (BLO), cottonseed meal (CSM), dehydrated cow manure (COW), and urea (UREA) factored with lettuce (Lactuca sativa L.) of different morphological phenotypes including iceberg, romaine, loose head, and loose leaf were studied in a greenhouse. Lettuce growth increased with increasing nitrogen (N) applications from 0 to 800?mg N/pot (kg), but the top application of BLO, CSM, or UREA suppressed yields. Lettuce grown with BLO, CSM, or UREA had higher concentrations of N than with COW. Nitrate-N concentration in leaves of all varieties exceeded some standards at high application of organic fertilizer or urea except for COW. In general, increasing N application resulted in higher concentration of NH₄-N in lettuce with increases in applications of fertilizers. Organic fertilizers and urea were equally effective in supporting growth and affecting nutrient accumulation in lettuce if sufficient N was supplied.  相似文献   

Improving chilling tolerance of rice seedling by seed priming with salicylic acid     

Farzin Pouramir-Dashtmian  Mohammad Khajeh-Hosseini  Masoud Esfahani 《Archives of Agronomy and Soil Science》2013,59(9):1291-1302

This study was carried out in the Seed Research Laboratory of the Department of Crop Science, Ferdowsi University of Mashhad, Iran, in summer 2011. Rice (cv. Khazar) seeds were soaked in 0, 20, 50, and 100 mg l^?1 salicylic acid (SA) aerated solutions for 24 h and then dried back to original moisture content and were sown in three temperatures (28°C as normal and 12°C and 8°C as chilling temperature conditions). In the first step, both treated and untreated seeds were sown at the optimum temperature (28°C). Then, to apply chilling temperature, 15 days after sowing, one set of the seeds was exposed to 12°C and 8°C under controlled conditions. Results showed that all seedlings raised from seeds primed with SA solutions produced a higher root and shoot length, final emergence percentage, and relative water content and also lower mean emergence time and time to 50% emergence (E50) in comparison with untreated seeds. Antioxidant enzyme system efficiently responded to the stress conditions and the priming treatment. Also seedling raised from primed seeds showed higher antioxidant activity at each temperature compared with control.  相似文献   

The Effect of Irrigation Management and Nitrogen Fertilizer On Grain Yield and Water-use Efficiency of Rice Cultivars in Northern Iran     

Eisapour Nakhjiri  Sajad  Ashouri  Majid  Sadeghi  Seyyed Mostafa  Mohammadian Roshan  Naser  Rezaei  Mojtaba 《Gesunde Pflanzen》2021,73(3):359-366

Gesunde Pflanzen - Alternate wetting and drying irrigation (AWDI) is a water-conserving strategy in rice fields. An experiment, conducted as a split-split plot design and based on...  相似文献   

Phenotypic and genetic parameter estimates for reproductive traits in Zandi sheep     

Kourosh Mohammadi  Mohammad Taghi Beigi Nassiri  Enayat Rahmatnejad  Masoud Sheikh  Jamal Fayazi  Amin Karimi Manesh 《Tropical animal health and production》2013,45(2):671-677

This study reports on the phenotypic and genetic (co)variance components for reproductive traits in Zandi sheep, using between 1,859 and 2,588 records obtained from 577 ewes. The data were collected from the Khojir Breeding Station of Zandi sheep in Tehran, Iran from 1994 to 2008. The basic traits were litter size at birth (LSB), litter size at weaning (LSW), litter mean weight per lamb born (LMWLB), and litter mean weight per lamb weaned (LMWLW), and the composite traits were total litter weight at birth (TLWB) and total litter weight at weaning (TLWW). Genetic analyses were carried out using the restricted maximum likelihood method that was explored by fitting the additive direct genetic effects and permanent environmental effects of the ewes as random effects and the ewe age at lambing and lambing year as fixed effects for all of the investigated traits. Akaike’s information criterion was used to choose the most appropriate model. LSB, LSW, LMWLB, LMWLW, TLWB, and TLWW direct heritability estimates were 0.07, 0.05, 0.12, 0.10, 0.08, and 0.14, respectively. The estimated fractions of variance due to the permanent environmental effects of the ewe ranged from 0.03 for LMWLB to 0.08 for LMWLW and TLWW. Corresponding repeatability estimates ranged from 0.10 for LSW to 0.22 for TLWW. Direct genetic correlations varied from ?0.61 for LSB–LMWLB to 0.88 for LSB–LSW and LSB–TLWB. Results indicate that genetic change depends not only on the heritability of traits, but also on the observed phenotypic variation; therefore, improvement of non-genetic factors should be included in the breeding programs.  相似文献   

Biochemical and physiological bases for utilization of dietary amino acids by young Pigs     

Reza Rezaei  Weiwei Wang  Zhenlong Wu  Zhaolai Dai  Junjun Wang  Guoyao WU 《畜牧与生物技术杂志(英文版)》2013,4(2):90-101

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Pregnancy in the Caspian Miniature Horse Using Frozen Semen Cryopreserved with the EquiPRO CryoGuard Freeze Medium and Customized Freezing Protocols     

Hiva Alipour  Mina Sharbatoghli  Poopak Eftekhari Yazdi  Abdolhossein Shahverdi  Mohamad Taghi Daneshzadeh  Masoud Afshani  Seied Jalal Mirian  Hormoz Hamidi  Ahmad Reza Mohammadi  Mojtaba Rezazadeh Valojerdi 《Journal of Equine Veterinary Science》2013

The primary goal of this project was to establish a protocol to freeze the sperm of the Caspian miniature horse in an attempt to start an intensive artificial insemination program to effectively increase the population of this breed, which has been listed as “Critical Rare Breed” by the American Livestock Breed Conservancy and is in danger of extinction. Commercially available equine freezing medium (EquiPRO CyoGuard Complete egg-yolk extender) was used for the initial setup of two different freeze protocols: slow and fast. The fast-freeze protocol had slightly better postthaw results and was used for a fertility demonstration. Five mares of proven fertility, aged 3 to 12 years, were used in the fertility trials, two of which resulted in pregnancy. This is the first report of pregnancy in the Caspian miniature horse using frozen semen, and the results seem to be a promising start to an extensive program to help this endangered breed, although further research on freezing protocols and conditions for this process are necessary to further improve the survival of semen and pregnancy rate.  相似文献   

Fate of Graywater Constituents After Long-Term Application for Landscape Irrigation     

Masoud Negahban-Azar  Sybil E. Sharvelle  Mary E. Stromberger  Christopher Olson  Larry A. Roesner 《Water, air, and soil pollution》2012,223(8):4733-4749

While interest in and adoption of graywater reuse for irrigation has rapidly grown in recent years, little is known about the long-term effects of graywater irrigation. Concerns exist in relation to the presence of pathogenic organisms, fate of personal care products, and accumulation of salts. The purpose of this research was to evaluate the long-term effects of graywater irrigation to soil quality under real conditions where homeowners may not always apply graywater in a highly controlled manner. Four households from different climatic and geological conditions were selected for sampling (AZ, CA, CO, and TX) where graywater was applied for irrigation for a minimum of 5?years. Soil samples were collected in areas irrigated with graywater and areas irrigated with freshwater within the same yard. Soil cores were taken at depths of 0?C15, 15?C30, and 30?C100?cm and analyzed separately for surfactants, antimicrobials, sodium adsorption ratio (SAR), electrical conductivity (EC), extractable boron, fecal indicator organisms (E. coli, enterococci, and Clostridium perfringens), and soil dehydrogenase activity. In surface soil samples (0?C15?cm), the average total surfactant concentration (over all sites) was higher in graywater-irrigated soil (0.078?±?0.033) compared to freshwater-irrigated soil (0.030?±?0.025?mg?kg^?1). This difference was not found to be significant (P?>?0.05). Triclosan and triclocarban were detected in surface soil samples at some locations (3.8?C6.3 and 3.5?C9.1???g?kg^?1, respectively), but not in samples deeper than 15?cm. Among the sampling locations, the TX household appeared to be most impacted by graywater, as evidenced by elevated SAR, potentially toxic levels of B, and relatively high numbers of E. coli and enterococci due to 30?years of graywater application for irrigation.  相似文献   

Evaluation of the relationship between endometriosis and omega-3 and omega-6 polyunsaturated fatty acids     

Khanaki K  Nouri M  Ardekani AM  Ghassemzadeh A  Shahnazi V  Sadeghi MR  Darabi M  Mehdizadeh A  Dolatkhah H  Saremi A  Imani AR  Rahimipour A 《Iranian Biomedical Journal》2012,16(1):38-43

Background: Endometriosis is a common chronic inflammation causing major problems including infertility. The role of omega-3 and omega-6 fatty acids as their potential anti-inflammatory effects in endometriosis needs to be further explored. The objective of this study was to compare serum phospholipid fatty acid profile in endometriosis patients with controls, and to explore the correlation of this profile with the severity of the disease. Methods: Sixty-four endometriosis patients and 74 control women, in reproductive age, participated in this study. Among the endometriosis patients, 19 cases were in stage I, 27 cases in stage II, 8 cases in stage III, and 10 cases in stage IV. Each patient underwent laparoscopy. Before surgery, 5 ml of blood was obtained. After extraction of the total lipids, serum total phospholipid fraction was isolated by thin layer chromatography. Fatty acid composition of the phospholipid fraction was determined by gas chromatography and the resulted profile was compared in endometriosis patients and controls. The profile was also compared in the endometriosis group based on the severity of disease. Results: Stearic acid was significantly lower in the endometriosis group as compared to controls (P= 0.030). No other fatty acid compositions were significantly different between patients and controls. Serum ratio of eicosapentaenoic acid (EPA) to arachidonic acid (AA) was in reasonable correlation with the severity of endometriosis (r = 0.34, P = 0.006). Conclusion: According to these findings, levels of fatty acids in serum total phospholipids seem not to be a marker for endometriosis, but the EPA to AA ratio was a relevant factor indicating severity of illness.Key Words: Endometriosis, Phospholipids, Omega-3 fatty acids  相似文献