The full length hepatitis C virus polyprotein and interactions with the interferon-beta signalling pathways in vitro     

Ghannad MS  Zamani A 《Iranian Biomedical Journal》2008,12(1):23-34

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Fabrication and Characterization of Nanocomposite Hydrogel Based on Alginate/Nano-Hydroxyapatite Loaded with Linum usitatissimum Extract as a Bone Tissue Engineering Scaffold     

Mahnaz Mohammadpour  Hadi Samadian  Nader Moradi  Zhila Izadi  Mahdieh Eftekhari  Masoud Hamidi  Amin Shavandi  Anthony Quro  Emmanuel Petit  Cdric Delattre  Redouan Elboutachfaiti 《Marine drugs》2022,20(1)

In the current paper, we fabricated, characterized, and applied nanocomposite hydrogel based on alginate (Alg) and nano-hydroxyapatite (nHA) loaded with phenolic purified extracts from the aerial part of Linum usitatissimum (LOH) as the bone tissue engineering scaffold. nHA was synthesized based on the wet chemical technique/precipitation reaction and incorporated into Alg hydrogel as the filler via physical cross-linking. The characterizations (SEM, DLS, and Zeta potential) revealed that the synthesized nHA possess a plate-like shape with nanometric dimensions. The fabricated nanocomposite has a porous architecture with interconnected pores. The average pore size was in the range of 100–200 µm and the porosity range of 80–90%. The LOH release measurement showed that about 90% of the loaded drug was released within 12 h followed by a sustained release over 48 h. The in vitro assessments showed that the nanocomposite possesses significant antioxidant activity promoting bone regeneration. The hemolysis induction measurement showed that the nanocomposites were hemocompatible with negligible hemolysis induction. The cell viability/proliferation confirmed the biocompatibility of the nanocomposites, which induced proliferative effects in a dose-dependent manner. This study revealed the fabricated nanocomposites are bioactive and osteoactive applicable for bone tissue engineering applications.  相似文献   

Body growth,intestinal morphology and microflora of quail on diets supplemented with micronised wheat fibre     

M. Rezaei  M. A. Karimi Torshizi  H. Wall  E. Ivarsson 《British poultry science》2018,59(4):422-429

1. Particle size reductions of fibre-rich materials alter structure, functional and digestive properties. To determine the effects of using fibre as an additive in Japanese quail rations on performance and gut physiology, a trial using micronised wheat fibre (MWF) at levels of 0.0, 5, 10 and 15 g/kg in feed was conducted.

2. Growth rate and feed efficiency were significantly improved when diets contained MWF while feed intake was not affected by levels of the fibre. As MWF content increased, the relative weight of gizzard and gastrointestinal tract (GIT) significantly increased whereas liver relative weight significantly decreased.

3. MWF inclusion significantly increased relative length of gut segments, villi height, villus thickness, the villi height to crypt depth proportion in jejunum and ileum and the number of goblet cells in different parts of intestine.

4. Tibia weight, length and ash content were increased linearly with rising MWF inclusion. Litter moisture was affected by MWF inclusions in a quadratic manner. The colony forming unit (CFU/g) of Streptococci spp. in ileal digesta was decreased with increasing MWF inclusion levels in the diet.

5. In conclusion, MWF can be used as a feed additive in quail diets and its inclusion in feed resulted in better performance, beneficial changes in intestinal microbial counts and improvements in small intestine morphology.  相似文献   

Determination of antibiotic resistance pattern and virulence genes in Escherichia coli isolated from bovine with subclinical mastitis in southwest of Iran     

Marashifard  Masoud  Karimi Aliabad  Zahra  Malek Hosseini  Seyed Ali Asghar  Darban-Sarokhalil  Davood  Mirzaii  Mehdi  Khoramrooz  Seyed Sajjad 《Tropical animal health and production》2019,51(3):575-580

The aims of the present study were to investigate the prevalence of some virulence genes and also determine the antimicrobial resistance pattern of E. coli isolated from bovine with subclinical mastitis. The milk of 502 cows was collected from 8 dairy herds in the southwest of Iran. Conventional biochemical tests were used for identification of E. coli at the species level. Antimicrobial susceptibility patterns of E. coli isolates were determined by disc agar diffusion method and polymerase chain reaction (PCR) was used for detection of seven virulence genes including f17A, afaE-8, afaD-8, eaeA, cnf1, cnf2, and iucD. Seventy (13.94%) isolates of E. coli were identified in 502 milk samples. The highest rate of resistance was observed against tetracycline (18.6%), while none of the isolates were resistant to streptomycin. Eight (11.5%) out of 70 E. coli isolates carried at least one of the virulence genes. The afaD-8 was the most prevalent gene detected in 5 (7.1%) isolates. The afaE-8, iucD, and eaeA were detected in 3, 3, and 2 isolates respectively. Low prevalence of virulence factors may be indicating that most of the E. coli isolates originated from the commensal flora of cows and enter to the udders via environment contamination with feces.

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The assessment of feto-maternal hemorrhage in an artificial model using anti-D and anti-fetal hemoglobin antibody by flow cytometry     

Pourazar A  Homayouni V  Rezaei A  Andalib A  Oreizi F 《Iranian Biomedical Journal》2008,12(1):43-48

BACKGROUND: When fetal red cells enter the maternal circulation from placenta, an event would be happened that is described as feto-maternal hemorrhage (FMH). This life-threatening condition could be detected by using RBC antigens (surface antigens and intracellular antigens). Therefore, the measurement of fetal RBC in an artificial model would be useful to calculate FMH and consequently the dosage of Rhogam for prophylaxis. The aim of the present study was to evaluate FMH in an artificial mixture model. METHODS: A series of 40 artificial specimens were prepared consisting of Rh(D) negative adult blood (non-immunized) spiked with varying amounts of Rh(D) positive cord blood from mothers between 20-30 years old in Shahid Beheshti Hospital, Tehran, Iran. Monoclonal anti-D and anti-HbF (fetal hemoglobin) were used for detection of fetal RBC in artificial mixture sample modeling. RESULTS: This study showed that the percentage of fetal cells in artificial sample for anti-D antigen is in ranges of 0.28%-0.32% for a 0.25% dilution mixture, and 1.3%-2.05% for the mixture with dilution 2%. In addition, the ranges of data for anti-HbF staining was obtained 0.2%-0.34% for the 0.25% dilution sample, and the ranges of 1.04-1.8% for the 2% dilution. The regression analysis indicated that the correlation of anti-D assessment with expected standard method was r2 = 0.9672 and anti-HbF assessment was r2 = 0.8842. CONCLUSION: Although both molecule targets could be used for detection of fetal RBC, in this model, anti-D staining was more accurate than anti-HbF staining. However, since anti-D can not be utilized for low-density or weak phenotype and other incompatibility, the anti-HbF labeling could be used for all FMH.  相似文献   

Characterization of phytoplasmas related to peanut witches’-broom and stolbur groups associated with alfalfa diseases in Iran     

Hosseini  Somayeh  Bahar  Masoud  Zirak  Leila 《植物病害和植物保护杂志》2013,120(2):70-76

Journal of Plant Diseases and Protection - In the central and south eastern provinces of Iran, alfalfa plants showing phytoplasma diseases-like symptoms categorized into three major types, namely...  相似文献   

Genetic variation,vegetative compatibility,and aggressiveness diversity of Diplodia bulgarica isolates from apple orchards in West Azarbaijan province of Iran     

Roya Zeynali Bari  Masoud Abrinbana  Youbert Ghosta 《Plant pathology》2021,70(6):1326-1341

This study investigated inter-simple sequence repeat (ISSR), vegetative compatibility, and aggressiveness diversity in 101 isolates of Diplodia bulgarica recovered from apple trees displaying symptoms of canker and decline in West Azarbaijan province of Iran. Marker analyses revealed high within population diversity, low genetic differentiation, high gene flow, and sharing of multilocus genotypes (MLGs) among geographic populations. Moreover, clustering and multivariate analyses identified two highly differentiated genetic clusters with limited admixture between them. These findings may suggest that the pathogen has been introduced from two genetically divergent sources and has been moved within the region through infected materials. The large number of MLGs, low clonal fraction, and absence of a widely distributed dominant genotype may explain the occurrence of recombination in this pathogen. However, significant linkage disequilibrium in the populations and limited admixture between genetic clusters may indicate the rare occurrence of recombination in D. bulgarica populations in West Azarbaijan, and that the pathogen has not been in the province long enough to reach equilibrium. Vegetative compatibility analyses revealed the occurrence of anastomosis between nonself pairings and high vegetative compatibility group diversity within populations. All studied MLGs produced necrotic lesions on detached shoots of Red Delicious apple but differed in their aggressiveness levels. Our results provide new insights into genetic and phenotypic variation of D. bulgarica that can assist in developing management strategies. Our findings also highlight the vital need for quarantine measures and the production of healthy plant materials to prevent the introduction and spread of the pathogen in Iran.  相似文献   

Changes of extracted bioactive compounds from brown algae (Cystoseira indica) after conversion to mill and tablet using a quantitative metabolomics approach     

Fasakhodi  Maliheh Taheri  Abed-Elmdoust  Amirreza  Mirvaghefi  Alireza  Hosseini  Seyed Vali  Tavabe  Kamran Rezaei 《Aquaculture International》2021,29(6):2793-2804

Aquaculture International - There is developing importance in the biomedical use of seaweeds such as brown algae, mostly due to their contents of bioactive substances. In the present study,...  相似文献   

Resistance spectra of wheat genotypes and virulence patterns of <Emphasis Type="Italic">Mycosphaerella graminicola</Emphasis> isolates in Iran     

Masoud Abrinbana  Javad Mozafari  Masoud Shams-bakhsh  Rahim Mehrabi 《Euphytica》2012,186(1):75-90

Co-evolution of wheat and its devastating pathogen Mycosphaerella graminicola (anamorph Septoria tritici), the causal agent of septoria tritici blotch, a foliar disease of wheat, is suggested to occur in Fertile Crescent as their center of origin and, thus, interaction between pathogen virulence and host resistance is important subject to be addressed. We have investigated resistance spectra of 54 wheat genotypes including a set of differentials carrying known resistance genes and virulence patterns of 14 M. graminicola isolates at seedling stage under controlled environmental conditions. The isolates were collected in Iran from five provinces. Diversity in virulence and aggressiveness was observed among the isolates from four provinces. Isolates collected from Golestan province were virulent to all wheat genotypes from germplasm of Iran, while specific resistances were identified to the isolates from other provinces. Among wheat genotypes, cvs. Chamran, Morvarid and Hirmand had the greatest number of specific resistances as well as partial resistance. Wheat genotypes of the differential set also differed in their reactions to the isolates. Arina, Flame and TE 9111 were specifically resistant to the greatest number of isolates from different provinces. Most isolates were virulent to the other differentials such as cvs. Shafir, Estanzuela federal and Courtot indicating that extensive adaption of virulence to most of the known resistance genes (Stb) has occurred in these regions. The new sources of resistance to highly virulent isolates from Iran may also be utilized in wheat breeding programs to develop resistant cultivars against pathogen populations in other countries.  相似文献   

Effect of body condition score on the testicular biometrical measures,semen characteristics and testosterone level in jackass     

Shimaa R. Masoud  Afaf A. Kishta  Omaima M. Kandil  Said I. Fathalla  Sherif M. Shawky  Ahmed Sabry S. Abdoon 《Reproduction in domestic animals》2021,56(12):1506-1510

This study was undertaken to evaluate the effect of body condition score (BCS) on testicle and epididymis biometrics, semen characteristics and testosterone level in Egyptian Jack. This study was conducted on 50 mature Jacks divided according to their body condition score into four groups: Poor (G1), moderate (G2), good (G3) and fat (G4). The complete testis was collected immediately after execution in the Giza Zoo abattoir; then, the epididymis was carefully dissected at the testicular junction. Biometrical measures including length, weight and volume were determined for the right and left testis and epididymis. Also, epididymal sperm was collected from all examined animas and evaluated for sperm concentration, progressive motility, viability and sperm abnormalities. Serum samples were collected for determination of total testosterone level. Results showed that the body condition score of the examined animal affects their biometrical measure of testicles and epididymis. There is a significant decrease (p < .05) in biometrical measures for the testicles and epididymis, sperm concentration, motility, viability and testosterone level in poor BCS animals (G1). The highest values were recorded in Good BCS (G3) Jacks. Conclusion: Jacks with good BCS (G3) should be selected for breeding activity in donkey.  相似文献