Pancytopenia in a cat with visceral leishmaniasis     

Ricardo Marcos  Marta Santos  Fernanda Malhão  Rui Pereira  Ana Cristina Fernandes  Luís Montenegro  Paola Roccabianca 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2009,38(2):201-205

Abstract: A 4‐year‐old, domestic shorthair, female spayed cat was presented for decreased appetite and depression. Severe pancytopenia with erythrocyte autoagglutination was found. The cat was seronegative for feline immunodeficiency and leukemia viruses. Immune‐mediated hemolytic anemia was suspected but no response to treatment with a blood transfusion, enrofloxacin, and prednisone was observed. Blood and bone marrow smears obtained 11 days later contained Leishmania amastigotes in the cytoplasm of neutrophils and macrophages, respectively. Serologic and PCR testing of peripheral blood confirmed infection with Leishmania infantum. Despite treatment, the cat worsened clinically and was euthanized. At necropsy, visceral dissemination of the parasite was confirmed. The findings in this case indicate that visceral leishmaniasis should be considered as a differential diagnoses in cats with pancytopenia in areas endemic for Leishmania. In addition, amastigotes may be observed in peripheral blood neutrophils.  相似文献   

Comparison between the analgesic effects of morphine and tramadol delivered epidurally in cats receiving a standardized noxious stimulation     

Douglas S. Castro  Marta F.A. Silva  Andre C. Shih  Pedro P.A. Motta  Marcos V.M. Pires  Paulo O. Scherer 《Journal of Feline Medicine and Surgery》2009,11(12):948-953

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Cutaneous transmissible venereal tumor without genital involvement in a prepubertal female dog     

Marcos R  Santos M  Marrinhas C  Rocha E 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2006,35(1):106-109

An 11-month-old prepubertal crossbreed female dog was presented with multiple nodular lesions disseminated over the cervical, back, flank, and abdominal regions. The lesions were ulcerated and cauliflowerlike, or nodular and subcutaneous, measuring up to 13 cm in diameter. Cytologic preparations of one of the lesions revealed a uniform population of round to oval cells, with lightly basophilic cytoplasm that contained multiple distinct vacuoles. Frequent mitotic figures and occasional lymphocytes were also observed. The cytologic diagnosis was cutaneous transmissible venereal tumor (TVT) in a progressing growth phase. This was confirmed by histologic and immunohistochemical findings. Vaginal TVT was diagnosed later in the dog's mother. TVT is a contagious neoplasm of sexually mature dogs that usually is transmitted by coitus and affects the genital mucosa. To our knowledge, this is the first report of naturally occurring multicentric TVT in a prepubertal female dog and also is unique in its exclusively cutaneous (no mucosal) involvement. We speculate that transmission of neoplastic cells occurred during cohabitation and social/mothering behavior between the dogs. Despite the atypical clinical presentation, response to chemotherapy with vincristine was excellent, leading to complete regression of the neoplasm without relapse after 6 months.  相似文献   

Antiparasitic efficacy of dietary administration of trichlorfon (Masoten®) in the control of Neoechinorhynchus buttnerae (Neochinorhynchidae) in Colossoma macropomum (Serrasalmidae)     

Castro  Liliane Araújo  Andrade-Porto  Sanny Maria  Oliveira  Rebeca Guimarães  Batista  Yasmin Luzeiro  Silva  Felipe Moura Araújo  Oliveira  Christiane Patrícia Feitosa  Affonso  Elizabeth Gusmão  Cruz  Claudinei  Tavares-Dias  Marcos 《Aquaculture International》2021,29(6):2477-2488

Aquaculture International - This study investigated the efficacy of trichlorfon against Neoechinorhynchus buttnerae, as well as its palatability, residue analysis, and the blood parameters of...  相似文献   

Unravelling pest infestation and biological control in low-input orchards: the case of apple blossom weevil     

Marcos Miñarro  Daniel García 《Journal of pest science》2018,91(3):1047-1061

Low-input farming is an alternative production system that provides a great opportunity to disentangle the natural mechanisms regulating crop pests, since neither pests nor their natural enemies are disrupted by pesticides. Here, we use a key apple pest in Europe, the apple blossom weevil (Anthonomus pomorum), as a model case to unravel the factors driving pest infestation and its biological control in a low-input context, namely the cider apple orchards of NW Spain. We applied a holistic approach based on the complete life cycle of the pest and combined large-scale observation (23 orchards) with small-scale experimental assessment. Weevil attack (0.4–37.4% of flowers) increased with the proportion on apple trees in the immediate orchard neighbourhood and with semi-natural woody habitat in the surrounding landscape and decreased with tree distance to orchard edge and apple bloom level. Thus, the prevalence of the pest depended on the availability of the various resources required for foraging, egg-lying and overwintering. Three types of natural enemies supplied complementary pest control by preying on weevils at different stages in their life cycle: seven parasitoid species attacked immature weevils (6.4–81.5%), while the additive effects of birds and crawling arthropods were evident in terms of the removal of adult weevils (31–44%). We conclude that the effective biological control of A. pomorum can be achieved in low-input systems to maintain the pest at non-harmful levels, through combined management of the pest, its habitat and its natural enemies.  相似文献   

Partial least squares regression (PLSR) associated with spectral response to predict soil attributes in transitional lithologies     

Marcos Rafael Nanni  Everson Cezar  Guilherme Fernando Capristo Silva  Anderson Antonio da Silva Gualberto 《Archives of Agronomy and Soil Science》2018,64(5):682-695

New techniques and improvements are required to quantify soil’s chemical and physical properties on production environment, reducing environmental impacts and minimizing soil analysis time. The aim of this study is to evaluate the possibility to estimate the content of silt, sand, clay, total iron and organic matter in soils formed by different lithologies in Parana State, Brazil, using VIS-NIR spectrum associated with Partial Least Square Regression (PLSR). 200 soil samples were collected in an area formed by Lixisols, Cambisols, Ferralsols, Arenosols and Nitisols in a depths of 0–0.2 and 0.2–0.8 m. Spectral readings were obtained in laboratory by FieldSpec 3 JR sensor. The spectral curves of the samples were correlated to the attributes through PLSR. The results obtained for sand in prediction were better when compared to the other attributes, presenting R² = 0.90, r = 0.95 and RPD = 2.3. Clay and total iron presented satisfactory results, mainly for RPD values, which were above 2.4. Based on the results, it can be concluded that the PLSR technique associated with the spectral response of the soils, was able to estimate sand, clay and total iron with accuracy in a region formed by reworked materials, derived from several lithologies.  相似文献   

Effect of increasing monensin sodium levels in diets with virginiamycin on the finishing of Nellore cattle          下载免费PDF全文

João Marcos B. Benatti  João Alexandrino Alves Neto  Ivanna M. de Oliveira  Flávio D. de Resende  Gustavo R. Siqueira 《Animal Science Journal》2017,88(11):1709-1714

This study evaluated the effect of increasing levels of monensin sodium (MON) in diets with virginiamycin (VM) on the finishing of feedlot cattle. Two hundred and eighty intact male Nellore cattle (348 ± 32 kg body weight, 22 months) received one of the following five diets: control diet (without additives); diet containing VM (25 mg per kg dry matter) combined with 0 (MON0), 10 (MON10), 20 (MON20) or 30 (MON30) mg MON per kg dry matter. During adaptation (28 days), the MON0 diet increased dietary net energy for maintenance and gain compared to the control diet (P = 0.04). The combination of additives linearly reduced dry matter intake, body weight and average daily gain (P < 0.01). Considering the total study period (110 days), there was a trend of greater net energy intake for maintenance (P = 0.09) and hot carcass weight (P = 0.06) for animals fed MON0 compared to the control diet. The combination of additives linearly reduced dry matter intake (P = 0.04) and linearly increased gain : feed and dietary net energy for maintenance and gain (P < 0.01). The combination of VM with MON at a dose of 30 mg/kg dry matter is recommended for Nellore feedlot cattle because it improves the efficiency of energy utilization.  相似文献   

Pulse pressure variation as a guide for volume expansion in dogs undergoing orthopedic surgery     

Denise T. Fantoni  Keila K. Ida  André M. Gimenes  Matheus M. Mantovani  Jacqueline R. Castro  Geni C.F. Patrício  Aline M. Ambrósio  Denise A. Otsuki 《Veterinary anaesthesia and analgesia》2017,44(4):710-718

Objective

To investigate whether pulse pressure variation (PPV) can predict fluid responsiveness in healthy dogs during clinical surgery.

Study design

Prospective clinical study.

Animals

Thirty-three isoflurane-anesthetized dogs with arterial hypotension during orthopedic surgery.

Methods

Fluid challenge with lactated Ringer's solution (15 mL kg^?1 in 15 minutes) was administered in mechanically ventilated dogs (tidal volume 10 mL kg^?1) with hypotension [mean arterial pressure (MAP) < 65 mmHg]. The volume expansion was considered effective if cardiac output (CO; transesophageal Doppler) increased by ≥ 15%. Cardiopulmonary data were analyzed using two-way ANOVA, receiver operating characteristics (ROC) curves and Spearman coefficient; p < 0.05 was considered significant.

Results

Effective volume expansion, mean ± standard deviation 42 ± 4% increase in CO (p < 0.0001) was observed in 76% of the dogs, resulting in a decrease in PPV (p < 0.0001) and increase in MAP (p < 0.0001), central venous pressure (CVP; p = 0.02) and ejection fraction (p < 0.0001) compared with before the fluid challenge. None of these changes occurred when volume expansion resulted in a nonsignificant CO increase of 4 ± 5%. No significant differences were observed in blood gas analysis between responsive and nonresponsive dogs. The increase in CO was correlated with the decrease in PPV (r = ?0.65; p < 0.0001) but absolute values of CO and PPV were not correlated. The PPV performance (ROC curve area: 0.89 ± 0.06, p = 0.0011) was better than that of CVP (ROC curve area: 0.54 ± 0.12) and MAP (ROC curve area: 0.59 ± 0.13) to predict fluid responsiveness. The best cut-off for PPV to distinguish responders and nonresponders was 15% (50% sensitivity and 96% specificity).

Conclusions and clinical relevance

In mechanically ventilated, healthy, isoflurane-anesthetized dogs, PPV predicted fluid responsiveness to volume expansion, and MAP and CVP did not show such applicability.  相似文献   

Biofilms of Candida spp. from the ocular conjunctiva of horses with reduced azole susceptibility: a complicating factor for the treatment of keratomycosis?          下载免费PDF全文

Raimunda Sâmia Nogueira Brilhante  Paula Vago Bittencourt  Débora de Souza Collares Castelo‐Branco  Glaucia Morgana de Melo Guedes  Jonathas Sales de Oliveira  Lucas Pereira Alencar  Rossana de Aguiar Cordeiro  Mariana Pinheiro  Evilázio Fernandes Nogueira‐Filho  Waldemiro de Aquino Pereira‐Neto  José Júlio Costa Sidrim  Marcos Fábio Gadelha Rocha 《Veterinary ophthalmology》2017,20(6):539-546

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Cell tube block: a new technique to produce cell blocks from fluid cytology samples          下载免费PDF全文

Ricardo Marcos  Marta Santos  Carla Marrinhas  Mario Caniatti 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2017,46(1):195-201

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